Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0007032

Physical Location & Seq

Genomic Coordinates Reverse (-)

36948794 .. 36957280

Gene Structure 8487 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002612

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3048 bp

ATGGCGGCCAGTGCGTATACGGATCAGCTGGAAGCTTACTTTCGGAGAGCGGATTTAGACGGAGATGGCAGGATCAGCGGAGCTGAGGCCGTTGCTTTCTTTCAGGGCGCCAATTTGCCCAAACCAGTTCTTGCTCAGATATGGATGCATGCAGATCAGAACAAAACTGGTTTCCTTGGTCGGCCAGAATTTTATAATGCTCTTAGACTTGTGACTGTGGCTCAAAGCAAGAGAGATTTAACGCCTGATATAGTCAAGGCCGCATTATATGGGCCAGCTGCTGCAAAAATTCCTGCTCCACAAATCAATCTTTCAGCCATATCTGCACCTCAAGCAAATCCTATGGCTGGTGCACCTGCTCCACAGATGGGTATAGGGACACCACCAACATCTCAAAGTTTTGGGTTTAGAGGACCAGGAGCTCCTAATGCAGGTATGAACCAAAATTATTTTCAGCCACAGCAAAACCAGTCCATGAGACCTCCTCAAGGCATGACCCCTGGGATGCCTACTAGTGGATATCCCCGTCCGCAACAGGGTGTGGGAGGTGGTATGGGGGGTCCCAATGTAATGAACTCAAATAATTGGCTTAGTGGAAGCACTGGTGCACCTCCTTCTGGTCCCAGAGGGATTAGTCCTTCCATGCCTTCATCAACAACACAACCACAACCTCCAGTGTCAACATCTTCCCTGCCGACAGTTAATGACTCTAAATCATTAGTTCCTTCTGGAAATGGGTTTGCATCCAATTCGGGTTTCTCAGGTGATTTATTTTCTGCAATCCCTGCTCAATCAAAGCCAGGAGCTCTTGGGTCAATATATTCTGCTAGTAGTGCACCTACGCCATCAGCCACTGTTCCAGTTTCAAGTGGGTCTCAATCTTCTAGTAAGCTCAATGCACTTGATTCACTGAGTGCATTCACAATGCAGCCTTCTGGTGGCCAATTTCAGCGGCCCCAGGCACCGTCAAACTCAAGCCCGCAAGTTTCAGCTGCGGGTCCTTCCTCTTTTGCATCACCTGCAATTTCAGTTGGAGTTGGAAACTCTACTTCTGAAAATTCACAGCCTCCTTGGCCAAAGATGAAGCCGTCTGATGTTCAGAAATATACGAAAGTCTTTATGGAAGTAGACAGTGACAGAGATGGGAAAATCACTGGAGAGCAGGCACGAAATCTATTTCTGAGCTGGAGGTTACCAAGAGAGGTTCTAAAGCAGGTGTGGGACTTATCTGATCAGGATAATGACAGCATGCTTTCCTTGAGAGAGTTCTGCTTTTCTCTCTATTTGATGGAACGTTACAGGGAAGGTCGGCCTCTTCCAGCTACACTTCCAAGCAATGTTATGTTGGATGAGACACTGCTGTCGATGACAGGTCAACCAAAAGTGGGTTATGGAAATGCAGCTTGGAGTCCCCACTCTGGCTTTGGACAACATCAAGGGATGCAAGGTTCCCAGATGATGCCACCTGGCACTGGTTTGAGGCCACCAATTCAGGGAAACGCCCCTCAGGCCGATCGTACAGTGCAACCGAATCAGCAAAATTTGAGGGTGCCAGGGATGGAGGGCTTGGCTCAAAACCAGCTTGACAATGGGAAGCAGGATTCAGCCAACTCAAGACATCAAGACCCTAACGGTGCTGGGAAAGAGGTTGAAGAAACTAAGAATGTGATTTTGGACTCAAGAGAGAAGATTGAGTTCTATCGCACGAAAATGCAGGAACTTGTTCTGTATAAAAGCAGATGTGATAACAGATTAAATGAGATCACAGAAAGGGCACTGGCAGACAAGCGTGAGGCTGAACTACTGGGTAAGAAGTATGAAGAGAAATATAAACAAGTGGCAGAAATAGCATCTAAGTTAACCATTGAAGAGGCAACGTTTCGTGAAGTTCAGGAGAGGAAGATGGAATTGCATCAAGCAATTGTTAAAATGGAGCAAGGAGGTAGTGCTGACGGTATCCTTCAGGTCCGAGCTGATCGTATACAATATGATCTTGAGGAGCTAATAAAGGCTCTTAATGAACGTTGCAAGAAACATGGGCTAGACATGAAGTCAGCTGCAATAATTGAGCTTCCAACTGGTTGGCAACCTGGAATTCAAGACGGAGCAGCTGTTTGGGACGAAGATTGGGACAAGTTCGAAGATGAAGGATTTGGCAATGATCTCATAATTGATTCCTCAACAAAATCTGTCCAGAGAGAAAAGGCTTCCCCAGATCGTAGTTCAACTGCTGATTCATCATCGGTTACTGATGGAAAGTCAGGAATTTCTTACAGCAATGGTGATCATGCACATGAAAGTGAATCTGTTTTCACCCACGGGGAAGATGAACATGTAAGAAGTCCCAATGGCAGTCTAGCTGGAAGAACTGCTGGAGACAGCCCGTCTCGAGACTTCTCAGACATCCACTATGGTAAAAATTCCGAAGCAGATGGAGAAACACACGGAAGTTTTGATGAATCGACCTGGGGTGCCTTTGACAATAATGATGATATTGATTCCGTTTGGGGATTTAATACTGACAAGGGCAAGGGCACAGATTCTGGGAAGCACAGAGATTTCTTTGGATCTGATGACTATGGTGCCAACCCAGTAAGAACTGGATCCCCCAATGCAGATACCGCGTTTCAGAAGAAGAGCATTTTCTTTGAAGACTCTGTTCCTAGCACACCAGTCTCAAGGTTTGCCAACTCGCCAAGATATAGTGAGGCCGGGGATCACTACTTTGACAATTTCTCAAGGTTTGATTCATTCAGCAGCAGGCAAGACAGTGGTTTTTCTTCTCAACCTGAGAGGTTCACAAGGTTTGATTCCATAAATAGCACTAGAGATTTTGGCCACACAAGGTTTGATTCCATAAGCAGCAGCAACGACTTTGGCCAAAGTCATGGCTTCACAAGGTTTGATTCCATAAACAGCACAAAAGATTCTGGCCAGAGTACATACTCTTTCGATGACACAGATCCATTTGGCTCTTCTGGCCCCTTTAAGGTCACATCAGAGAGTCAAAATCCAAAGAAGGGATCTGATAATTGGAATGCTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000147 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005938 GO:0006810 GO:0006886 GO:0006897 GO:0006996 GO:0007010 GO:0008104 GO:0008150 GO:0009987 GO:0015031 GO:0015629 GO:0015833 GO:0016043 GO:0016192 GO:0022607 GO:0030029 GO:0030036 GO:0030479 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0033036 GO:0034613 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044396 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045184 GO:0046872 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061645 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0098657 GO:0099568

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

EF-hand_4 EF-hand_5

Protein Analysis

1016

Amino Acids

109.84

Weight (kDa)

5.24

Isoelectric Point (pI)

48.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EH	PF12763	21 - 76	2.7e-07	EH domain
EH	PF12763	362 - 444	3.5e-12	EH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007032)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20760
fragaria_vesca	FvH4_5g25580 FvH4_5g25580
malus_domestica	MD06G1007400.v1.1
prunus_persica	Prupe.5G009300_v2.0.a1 Prupe.5G009300_v2.0.a1
pyrus_communis	pycom06g00650 pycom06g00660
rosa_chinensis	RchiOBHm_Chr7g0215891 RchiOBHm_Chr7g0219801 RchiOBHm_Chr7g0219941
rosa_laevigata	RLG00000002612
rosa_multiflora	Rmu_co8383945.1_g000001 Rmu_sc0006240.1_g000011
rosa_roxburghii	Rroxscaffold_3G00243830
rosa_rugosa	Rorug07G0156400 Rorug07G0156500
rosa_samantha	Rh7AG294500 Rh7AG298200 Rh7BG286300 Rh7CG314500 Rh7CG318200 Rh7CG318300 Rh7DG297400
rosa_wichuraiana	Rw7G025020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	195
AarI	CACCTGC	3 cut(s)	364, 1027, 1204
AasI	GACNNNNNNGTC	1 cut(s)	1360
Acc36I	ACCTGC	4 cut(s)	364, 422, 1027, 1204
AccB1I	GGYRCC	5 cut(s)	107, 961, 1549, 2471, 2582
AccBSI	CCGCTC	1 cut(s)	50
AccI	GTMKAC	3 cut(s)	17, 1128, 1981
AccII	CGCG	1 cut(s)	2624
AciI	CCGC	9 cut(s)	5, 50, 78, 261, 530, 952, 980, 995, 2622
AclI	AACGTT	3 cut(s)	1294, 1877, 2023
AclWI	GGATC	8 cut(s)	30, 80, 2575, 2598, 2611, 2724, 2957, 3031
AcoI	YGGCCR	7 cut(s)	6, 182, 940, 1073, 2836, 2878, 2932
AcsI	RAATTY	7 cut(s)	188, 288, 1057, 1540, 2094, 2265, 2419
AcuI	CTGAAG	1 cut(s)	1946
AcyI	GRCGYC	1 cut(s)	108
AdeI	CACNNNGTG	1 cut(s)	914
AfaI	GTAC	2 cut(s)	1519, 2941
AflIII	ACRYGT	1 cut(s)	2332
AgsI	TTSAA	6 cut(s)	867, 1652, 1868, 2099, 2226, 2651
AhdI	GACNNNNNGTC	2 cut(s)	2050, 2383
AhlI	ACTAGT	1 cut(s)	512
AjnI	CCWGG	8 cut(s)	415, 499, 799, 957, 1465, 1552, 2089, 2465
AjuI	GAANNNNNNNTTGG	8 cut(s)	435, 467, 740, 772, 1387, 1419, 1655, 1687
AloI	GAACNNNNNNTCC	2 cut(s)	1432, 1464
Alw21I	GWGCWC	5 cut(s)	355, 424, 610, 808, 838
Alw26I	GTCTC	7 cut(s)	472, 879, 1346, 2370, 2385, 2391, 2680
Alw44I	GTGCAC	3 cut(s)	351, 606, 834
AlwI	GGATC	8 cut(s)	30, 80, 2575, 2598, 2611, 2724, 2957, 3031
AlwNI	CAGNNNCTG	3 cut(s)	281, 854, 2543
Ama87I	CYCGRG	1 cut(s)	2388
ApaLI	GTGCAC	3 cut(s)	351, 606, 834
ApoI	RAATTY	7 cut(s)	188, 288, 1057, 1540, 2094, 2265, 2419
Asp700I	GAANNNNTTC	2 cut(s)	1722, 3041
AspLEI	GCGC	1 cut(s)	110
AspS9I	GGNCC	8 cut(s)	272, 413, 560, 620, 954, 998, 1966, 2981
AsuC2I	CCSGG	1 cut(s)	2713
AsuHPI	GGTGA	4 cut(s)	776, 1008, 2294, 2305
AsuII	TTCGAA	1 cut(s)	2139
AvaI	CYCGRG	1 cut(s)	2388
AvaII	GGWCC	5 cut(s)	413, 560, 620, 998, 1966
AxyI	CCTNAGG	1 cut(s)	1506
BaeGI	GKGCMC	5 cut(s)	355, 610, 838, 1777, 2537
BalI	TGGCCA	5 cut(s)	942, 1075, 2838, 2880, 2934
BamHI	GGATCC	1 cut(s)	2603
BanI	GGYRCC	5 cut(s)	107, 961, 1549, 2471, 2582
BanII	GRGCYC	2 cut(s)	424, 808
BbsI	GAAGAC	1 cut(s)	2658
Bbv12I	GWGCWC	5 cut(s)	355, 424, 610, 808, 838
BbvCI	CCTCAGC	1 cut(s)	84
BccI	CCATC	9 cut(s)	59, 361, 853, 1136, 1282, 1552, 1897, 2246, 2426
BceAI	ACGGC	2 cut(s)	74, 1072
BcgI	CGANNNNNNTGC	2 cut(s)	732, 766
BciT130I	CCWGG	8 cut(s)	417, 501, 801, 959, 1467, 1554, 2091, 2467
BciVI	GTATCC	1 cut(s)	1967
BclI	TGATCA	2 cut(s)	1231, 2284
BcnI	CCSGG	1 cut(s)	2713
BcoDI	GTCTC	7 cut(s)	472, 879, 1346, 2370, 2385, 2391, 2680
BcuI	ACTAGT	1 cut(s)	512
BfaI	CTAG	7 cut(s)	513, 828, 885, 2042, 2357, 2664, 2826
BfoI	RGCGCY	1 cut(s)	111
BfuAI	ACCTGC	4 cut(s)	364, 422, 1027, 1204
BfuI	GTATCC	1 cut(s)	1967
BglI	GCCNNNNNGGC	2 cut(s)	1072, 1508
Bme1390I	CCNGG	9 cut(s)	417, 501, 801, 959, 1467, 1554, 2091, 2467, 2713
Bme18I	GGWCC	5 cut(s)	413, 560, 620, 998, 1966
BmeRI	GACNNNNNGTC	2 cut(s)	2050, 2383
BmeT110I	CYCGRG	1 cut(s)	2388
BmgT120I	GGNCC	8 cut(s)	272, 413, 560, 620, 954, 998, 1966, 2981
BmrFI	CCNGG	9 cut(s)	417, 501, 801, 959, 1467, 1554, 2091, 2467, 2713
BmrI	ACTGGG	2 cut(s)	1814, 2585
BmsI	GCATC	8 cut(s)	135, 495, 752, 1022, 1431, 1449, 1859, 1921
BmuI	ACTGGG	2 cut(s)	1814, 2585
BpiI	GAAGAC	1 cut(s)	2658
BplI	GAGNNNNNCTC	2 cut(s)	469, 501
BpmI	CTGGAG	4 cut(s)	657, 1176, 1207, 2394
Bpu10I	CCTNAGC	1 cut(s)	84
Bpu14I	TTCGAA	1 cut(s)	2139
BpuEI	CTTGAG	9 cut(s)	315, 471, 958, 1279, 1597, 1663, 2015, 2662, 2722
BpuMI	CCSGG	1 cut(s)	2713
BsaHI	GRCGYC	1 cut(s)	108
BsaI	GGTCTC	2 cut(s)	472, 879
BsaJI	CCNNGG	9 cut(s)	175, 499, 500, 957, 1070, 1553, 2317, 2466, 2712
BsaXI	ACNNNNNCTCC	2 cut(s)	2097, 2127
Bse21I	CCTNAGG	1 cut(s)	1506
Bse3DI	GCAATG	3 cut(s)	1342, 2164, 2284
BseBI	CCWGG	8 cut(s)	417, 501, 801, 959, 1467, 1554, 2091, 2467
BseDI	CCNNGG	9 cut(s)	175, 499, 500, 957, 1070, 1553, 2317, 2466, 2712
BseGI	GGATG	7 cut(s)	150, 510, 743, 1354, 1446, 1563, 2403
BseMI	GCAATG	3 cut(s)	1342, 2164, 2284
BseMII	CTCAG	8 cut(s)	75, 149, 774, 902, 1172, 1520, 2412, 2781
BseRI	GAGGAG	2 cut(s)	474, 2012
BseSI	GKGCMC	5 cut(s)	355, 610, 838, 1777, 2537
BseYI	CCCAGC	1 cut(s)	1637
BsgI	GTGCAG	1 cut(s)	309
Bsh1236I	CGCG	1 cut(s)	2624
Bsh1285I	CGRYCG	1 cut(s)	1516
BshNI	GGYRCC	5 cut(s)	107, 961, 1549, 2471, 2582
BsiEI	CGRYCG	1 cut(s)	1516
BsiHKAI	GWGCWC	5 cut(s)	355, 424, 610, 808, 838
BsiHKCI	CYCGRG	1 cut(s)	2388
BsiSI	CCGG	1 cut(s)	2712
BslFI	GGGAC	8 cut(s)	391, 546, 606, 1235, 1395, 2132, 2144, 2328
BsmAI	GTCTC	7 cut(s)	472, 879, 1346, 2370, 2385, 2391, 2680
BsmBI	CGTCTC	1 cut(s)	2391
BsmFI	GGGAC	8 cut(s)	391, 546, 606, 1235, 1395, 2132, 2144, 2328
BsmI	GAATGC	2 cut(s)	917, 3043
Bso31I	GGTCTC	2 cut(s)	472, 879
BsoBI	CYCGRG	1 cut(s)	2388
Bsp119I	TTCGAA	1 cut(s)	2139
Bsp1286I	GDGCHC	7 cut(s)	355, 424, 610, 808, 838, 1777, 2537
BspACI	CCGC	9 cut(s)	5, 50, 78, 261, 530, 952, 980, 995, 2622
BspCNI	CTCAG	8 cut(s)	76, 148, 773, 903, 1173, 1519, 2411, 2782
BspFNI	CGCG	1 cut(s)	2624
BspMI	ACCTGC	4 cut(s)	364, 422, 1027, 1204
BspPI	GGATC	8 cut(s)	30, 80, 2575, 2598, 2611, 2724, 2957, 3031
BspQI	GCTCTTC	2 cut(s)	2630, 2980
BspT104I	TTCGAA	1 cut(s)	2139
BspT107I	GGYRCC	5 cut(s)	107, 961, 1549, 2471, 2582
BspTNI	GGTCTC	2 cut(s)	472, 879
BsrBI	CCGCTC	1 cut(s)	50
BsrDI	GCAATG	3 cut(s)	1342, 2164, 2284
BssECI	CCNNGG	9 cut(s)	175, 499, 500, 957, 1070, 1553, 2317, 2466, 2712
BssNAI	GTATAC	2 cut(s)	18, 1982
BssNI	GRCGYC	1 cut(s)	108
BssT1I	CCWWGG	2 cut(s)	175, 1070
Bst1107I	GTATAC	2 cut(s)	18, 1982
Bst2UI	CCWGG	8 cut(s)	417, 501, 801, 959, 1467, 1554, 2091, 2467
Bst4CI	ACNGT	9 cut(s)	217, 700, 856, 966, 1133, 1522, 1634, 1955, 2771
Bst6I	CTCTTC	5 cut(s)	1320, 1815, 1863, 2630, 2980
BstACI	GRCGYC	1 cut(s)	108
BstAPI	GCANNNNNTGC	2 cut(s)	785, 1019
BstBI	TTCGAA	1 cut(s)	2139
BstC8I	GCNNGC	6 cut(s)	150, 276, 980, 1164, 1250, 2762
BstDSI	CCRYGG	1 cut(s)	2317
BstEII	GGTNACC	1 cut(s)	1191
BstENI	CCTNNNNNAGG	2 cut(s)	429, 486
BstF5I	GGATG	7 cut(s)	150, 510, 743, 1354, 1446, 1563, 2403
BstFNI	CGCG	1 cut(s)	2624
BstH2I	RGCGCY	1 cut(s)	111
BstHHI	GCGC	1 cut(s)	110
BstMAI	GTCTC	7 cut(s)	472, 879, 1346, 2370, 2385, 2391, 2680
BstMCI	CGRYCG	1 cut(s)	1516
BstNI	CCWGG	8 cut(s)	417, 501, 801, 959, 1467, 1554, 2091, 2467
BstNSI	RCATGY	3 cut(s)	152, 1252, 2336
BstPI	GGTNACC	1 cut(s)	1191
BstSCI	CCNGG	9 cut(s)	415, 499, 799, 957, 1465, 1552, 2089, 2465, 2711
BstSLI	GKGCMC	5 cut(s)	355, 610, 838, 1777, 2537
BstUI	CGCG	1 cut(s)	2624
BstV2I	GAAGAC	1 cut(s)	2658
BstX2I	RGATCY	4 cut(s)	2567, 2603, 2962, 3023
BstXI	CCANNNNNNTGG	2 cut(s)	2082, 2888
BstYI	RGATCY	4 cut(s)	2567, 2603, 2962, 3023
BstZ17I	GTATAC	2 cut(s)	18, 1982
Bsu36I	CCTNAGG	1 cut(s)	1506
BsuI	GTATCC	1 cut(s)	1967
BtgI	CCRYGG	1 cut(s)	2317
BtsCI	GGATG	7 cut(s)	150, 510, 743, 1354, 1446, 1563, 2403
BtsI	GCAGTG	1 cut(s)	1355
BveI	ACCTGC	4 cut(s)	364, 422, 1027, 1204
Cac8I	GCNNGC	6 cut(s)	150, 276, 980, 1164, 1250, 2762
CaiI	CAGNNNCTG	3 cut(s)	281, 854, 2543
CfoI	GCGC	1 cut(s)	110
Cfr13I	GGNCC	8 cut(s)	272, 413, 560, 620, 954, 998, 1966, 2981
Csp6I	GTAC	2 cut(s)	1518, 2940
CviQI	GTAC	2 cut(s)	1518, 2940
DinI	GGCGCC	1 cut(s)	109
DraIII	CACNNNGTG	1 cut(s)	914
DrdI	GACNNNNNNGTC	1 cut(s)	1360
DriI	GACNNNNNGTC	2 cut(s)	2050, 2383
DseDI	GACNNNNNNGTC	1 cut(s)	1360
EaeI	YGGCCR	7 cut(s)	6, 182, 940, 1073, 2836, 2878, 2932
Eam1104I	CTCTTC	5 cut(s)	1320, 1815, 1863, 2630, 2980
Eam1105I	GACNNNNNGTC	2 cut(s)	2050, 2383
EarI	CTCTTC	5 cut(s)	1320, 1815, 1863, 2630, 2980
Ecl136II	GAGCTC	2 cut(s)	422, 806
Eco130I	CCWWGG	2 cut(s)	175, 1070
Eco24I	GRGCYC	2 cut(s)	424, 808
Eco31I	GGTCTC	2 cut(s)	472, 879
Eco32I	GATATC	1 cut(s)	521
Eco47I	GGWCC	5 cut(s)	413, 560, 620, 998, 1966
Eco53kI	GAGCTC	2 cut(s)	422, 806
Eco57I	CTGAAG	1 cut(s)	1946
Eco81I	CCTNAGG	1 cut(s)	1506
Eco88I	CYCGRG	1 cut(s)	2388
Eco91I	GGTNACC	1 cut(s)	1191
EcoICRI	GAGCTC	2 cut(s)	422, 806
EcoNI	CCTNNNNNAGG	2 cut(s)	429, 486
EcoO109I	RGGNCCY	2 cut(s)	560, 998
EcoO65I	GGTNACC	1 cut(s)	1191
EcoRI	GAATTC	1 cut(s)	2094
EcoRII	CCWGG	8 cut(s)	415, 499, 799, 957, 1465, 1552, 2089, 2465
EcoRV	GATATC	1 cut(s)	521
EcoT14I	CCWWGG	2 cut(s)	175, 1070
EcoT22I	ATGCAT	1 cut(s)	150
EcoT38I	GRGCYC	2 cut(s)	424, 808
EgeI	GGCGCC	1 cut(s)	109
EheI	GGCGCC	1 cut(s)	109
ErhI	CCWWGG	2 cut(s)	175, 1070
Esp3I	CGTCTC	1 cut(s)	2391
FaqI	GGGAC	8 cut(s)	391, 546, 606, 1235, 1395, 2132, 2144, 2328
FauI	CCCGC	2 cut(s)	987, 988
FbaI	TGATCA	2 cut(s)	1231, 2284
FblI	GTMKAC	3 cut(s)	17, 1128, 1981
FokI	GGATG	7 cut(s)	157, 517, 730, 1361, 1453, 1570, 2390
FriOI	GRGCYC	2 cut(s)	424, 808
FspBI	CTAG	7 cut(s)	513, 828, 885, 2042, 2357, 2664, 2826
GlaI	GCGC	1 cut(s)	109
GsaI	CCCAGC	1 cut(s)	1641
GsuI	CTGGAG	4 cut(s)	657, 1176, 1207, 2394
HaeII	RGCGCY	1 cut(s)	111
HapII	CCGG	1 cut(s)	2712
HhaI	GCGC	1 cut(s)	110
Hin1I	GRCGYC	1 cut(s)	108
Hin6I	GCGC	1 cut(s)	108
HinP1I	GCGC	1 cut(s)	108
HincII	GTYRAC	3 cut(s)	681, 1376, 1860
HindII	GTYRAC	3 cut(s)	681, 1376, 1860
HindIII	AAGCTT	1 cut(s)	33
HpaI	GTTAAC	1 cut(s)	1860
HpaII	CCGG	1 cut(s)	2712
HphI	GGTGA	4 cut(s)	776, 1008, 2294, 2305
HpyCH4III	ACNGT	9 cut(s)	217, 700, 856, 966, 1133, 1522, 1634, 1955, 2771
HpyCH4IV	ACGT	3 cut(s)	1294, 1877, 2023
HpySE526I	ACGT	3 cut(s)	1294, 1877, 2023
Hsp92I	GRCGYC	1 cut(s)	108
HspAI	GCGC	1 cut(s)	108
KasI	GGCGCC	1 cut(s)	107
KflI	GGGWCCC	1 cut(s)	560
Ksp22I	TGATCA	2 cut(s)	1231, 2284
KspAI	GTTAAC	1 cut(s)	1860
LguI	GCTCTTC	2 cut(s)	2630, 2980
LmnI	GCTCC	9 cut(s)	80, 301, 364, 419, 427, 803, 1933, 1999, 2105
LweI	GCATC	8 cut(s)	135, 495, 752, 1022, 1431, 1449, 1859, 1921
MaeI	CTAG	7 cut(s)	513, 828, 885, 2042, 2357, 2664, 2826
MaeII	ACGT	3 cut(s)	1294, 1877, 2023
MaeIII	GTNAC	6 cut(s)	211, 1133, 1191, 1295, 2245, 2992
MbiI	CCGCTC	1 cut(s)	50
MfeI	CAATTG	1 cut(s)	1920
MflI	RGATCY	4 cut(s)	2567, 2603, 2962, 3023
MhlI	GDGCHC	7 cut(s)	355, 424, 610, 808, 838, 1777, 2537
MlsI	TGGCCA	5 cut(s)	942, 1075, 2838, 2880, 2934
MluNI	TGGCCA	5 cut(s)	942, 1075, 2838, 2880, 2934
Mly113I	GGCGCC	1 cut(s)	108
MlyI	GAGTC	5 cut(s)	701, 1417, 1670, 2648, 3013
MmeI	TCCRAC	4 cut(s)	1012, 1018, 1326, 2099
Mox20I	TGGCCA	5 cut(s)	942, 1075, 2838, 2880, 2934
Mph1103I	ATGCAT	1 cut(s)	150
MroXI	GAANNNNTTC	2 cut(s)	1722, 3041
MscI	TGGCCA	5 cut(s)	942, 1075, 2838, 2880, 2934
MseI	TTAA	8 cut(s)	239, 702, 1754, 1859, 1926, 2016, 2514, 2988
MslI	CAYNNNNRTG	3 cut(s)	1709, 2292, 2298
Msp20I	TGGCCA	5 cut(s)	942, 1075, 2838, 2880, 2934
MspA1I	CMGCKG	7 cut(s)	28, 78, 278, 952, 992, 2057, 2111
MspI	CCGG	1 cut(s)	2712
MspR9I	CCNGG	9 cut(s)	417, 501, 801, 959, 1467, 1554, 2091, 2467, 2713
MunI	CAATTG	1 cut(s)	1920
Mva1269I	GAATGC	2 cut(s)	917, 3043
MvaI	CCWGG	8 cut(s)	417, 501, 801, 959, 1467, 1554, 2091, 2467
MvnI	CGCG	1 cut(s)	2624
NarI	GGCGCC	1 cut(s)	108
NciI	CCSGG	1 cut(s)	2713
NmuCI	GTSAC	3 cut(s)	211, 1133, 2992
NsiI	ATGCAT	1 cut(s)	150
NspI	RCATGY	3 cut(s)	152, 1252, 2336
NspV	TTCGAA	1 cut(s)	2139
PaeI	GCATGC	2 cut(s)	152, 1252
PaeR7I	CTCGAG	1 cut(s)	2388
PaqCI	CACCTGC	3 cut(s)	364, 1027, 1204
PasI	CCCWGGG	1 cut(s)	500
PciI	ACATGT	1 cut(s)	2332
PciSI	GCTCTTC	2 cut(s)	2630, 2980
PctI	GAATGC	2 cut(s)	917, 3043
PdmI	GAANNNNTTC	2 cut(s)	1722, 3041
Ple19I	CGATCG	1 cut(s)	1516
PleI	GAGTC	5 cut(s)	701, 1416, 1670, 2648, 3012
PluTI	GGCGCC	1 cut(s)	111
PpsI	GAGTC	5 cut(s)	701, 1416, 1670, 2648, 3012
PpuMI	RGGWCCY	2 cut(s)	560, 998
PscI	ACATGT	1 cut(s)	2332
PsiI	TTATAA	1 cut(s)	195
Psp124BI	GAGCTC	2 cut(s)	424, 808
Psp1406I	AACGTT	3 cut(s)	1294, 1877, 2023
Psp5II	RGGWCCY	2 cut(s)	560, 998
Psp6I	CCWGG	8 cut(s)	415, 499, 799, 957, 1465, 1552, 2089, 2465
PspEI	GGTNACC	1 cut(s)	1191
PspFI	CCCAGC	1 cut(s)	1637
PspGI	CCWGG	8 cut(s)	415, 499, 799, 957, 1465, 1552, 2089, 2465
PspPI	GGNCC	8 cut(s)	272, 413, 560, 620, 954, 998, 1966, 2981
PspPPI	RGGWCCY	2 cut(s)	560, 998
PstNI	CAGNNNCTG	3 cut(s)	281, 854, 2543
PsuI	RGATCY	4 cut(s)	2567, 2603, 2962, 3023
PvuI	CGATCG	1 cut(s)	1516
PvuII	CAGCTG	5 cut(s)	28, 278, 992, 2057, 2111
RsaI	GTAC	2 cut(s)	1519, 2941
RsaNI	GTAC	2 cut(s)	1518, 2940
RseI	CAYNNNNRTG	3 cut(s)	1709, 2292, 2298
SacI	GAGCTC	2 cut(s)	424, 808
SapI	GCTCTTC	2 cut(s)	2630, 2980
SaqAI	TTAA	8 cut(s)	239, 702, 1754, 1859, 1926, 2016, 2514, 2988
Sau96I	GGNCC	8 cut(s)	272, 413, 560, 620, 954, 998, 1966, 2981
SchI	GAGTC	5 cut(s)	701, 1417, 1670, 2648, 3013
ScrFI	CCNGG	9 cut(s)	417, 501, 801, 959, 1467, 1554, 2091, 2467, 2713
SduI	GDGCHC	7 cut(s)	355, 424, 610, 808, 838, 1777, 2537
SfaNI	GCATC	8 cut(s)	135, 495, 752, 1022, 1431, 1449, 1859, 1921
SfoI	GGCGCC	1 cut(s)	109
Sfr274I	CTCGAG	1 cut(s)	2388
SfuI	TTCGAA	1 cut(s)	2139
SinI	GGWCC	5 cut(s)	413, 560, 620, 998, 1966
SlaI	CTCGAG	1 cut(s)	2388
SmiMI	CAYNNNNRTG	3 cut(s)	1709, 2292, 2298
SpeI	ACTAGT	1 cut(s)	512
SphI	GCATGC	2 cut(s)	152, 1252
SsiI	CCGC	9 cut(s)	5, 50, 78, 261, 530, 952, 980, 995, 2622
SspDI	GGCGCC	1 cut(s)	107
SspMI	CTAG	7 cut(s)	513, 828, 885, 2042, 2357, 2664, 2826
SstI	GAGCTC	2 cut(s)	424, 808
StyD4I	CCNGG	9 cut(s)	415, 499, 799, 957, 1465, 1552, 2089, 2465, 2711
StyI	CCWWGG	2 cut(s)	175, 1070
TaaI	ACNGT	9 cut(s)	217, 700, 856, 966, 1133, 1522, 1634, 1955, 2771
TaiI	ACGT	3 cut(s)	1297, 1880, 2026
TaqI	TCGA	5 cut(s)	1364, 2139, 2389, 2462, 2952
TatI	WGTACW	1 cut(s)	2939
TauI	GCSGC	3 cut(s)	8, 263, 955
Tru1I	TTAA	8 cut(s)	239, 702, 1754, 1859, 1926, 2016, 2514, 2988
Tru9I	TTAA	8 cut(s)	239, 702, 1754, 1859, 1926, 2016, 2514, 2988
TseFI	GTSAC	3 cut(s)	211, 1133, 2992
Tsp45I	GTSAC	3 cut(s)	211, 1133, 2992
TspGWI	ACGGA	5 cut(s)	35, 75, 2118, 2460, 2491
VneI	GTGCAC	3 cut(s)	351, 606, 834
VpaK11BI	GGWCC	5 cut(s)	413, 560, 620, 998, 1966
XagI	CCTNNNNNAGG	2 cut(s)	429, 486
XapI	RAATTY	7 cut(s)	188, 288, 1057, 1540, 2094, 2265, 2419
XceI	RCATGY	3 cut(s)	152, 1252, 2336
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1421, 1586
XhoI	CTCGAG	1 cut(s)	2388
XmiI	GTMKAC	3 cut(s)	17, 1128, 1981
XmnI	GAANNNNTTC	2 cut(s)	1722, 3041
XspI	CTAG	7 cut(s)	513, 828, 885, 2042, 2357, 2664, 2826
Zsp2I	ATGCAT	1 cut(s)	150

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.