Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0007032

Physical Location & Seq

Genomic Coordinates Forward (+)

31265131 .. 31273555

Gene Structure 8425 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G025020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3114 bp

ATGGCGGCCGGTGCGTACACGGATCAGCTGGAAGCTCACTTTCGGAGAGCGGATTTGGACGGAGATGGCAGGATCAGCGGAGCTGAGGCCGTCGCTTTCTTTCAGGGCGCCAATTTGCCCAAACCAGTTCTTGCTCAGATATGGATGCATGCGGATCAGAACAAAACTGGTTTCCTTGGTCGGCCAGAATTTTATAATGCTCTTAGACTTGTGACTGTGGCTCAAAGCAAGAGAGATTTAACGCCTGATATAGTCAAGGCCGCATTATACGGGCCAGCTGCTGCAAAAATTCCTGCTCCACAAATCAATCTTTCAGCCATATCTGCACCTCAAGCAAATCCTATGGCTGGTGCACCTGCTCCACAGATGGGTATGGGGACGCCACCAACATCTCAAAGTTTTGGGTTTAGAGGACCAGGAGCTCCTAATGCAGGTATGAACCAGAATTATTTTCAGCCACAGCAAAACCAGTCCATGAGACCTCCTCAAGGCATGACCCCTGGGATGCCTACTAGTGGATATTCCCGTCCACAACAGGGTGTGGGAGGTGGTATGGGGGGTCCCAATGTAATGAACTCAAATAATTGGCTTAGTGGAAGCACTGGTGCACCTCCTTCTGGTCCAAGAGGGATTAGTCCTTCCATGCCCTCATCTACAACACAACCACAACCTCCAGTGTCAACATCTTCCCTGGCAACAGTGAATGACTCTAAATCATTAGTTCCTTCTGGAAATGGGTTTGCATCCAATTCGGGTTTCTCAGGTGATTTATTTTCTGCAGCCCCTGCTCAATCAAAGCCAGGAGCTCCTGGGTCAACATATTCTGCTAGTAGTGCACCTACGCCATCAGCCATTGTTCCAGTTTCAAGTGGGTCCCAATCTTCTAGTAAGCTCAATGCACTTGATTCACTGAGTGCATTCACAATGCAGCCTTCTGGTGGCCAATTTCAGCGGCCCCAGGCACCGTCAAACTCAAGCCCGCAAGTTTCAGCTGCGGGTCCTTCCTCTTTTGCATCACCTGCAATTTCAGTTGGAGTCGGAAACTCTACTTCTGAAAATTCACAGCCTCCTTGGCCAAAGATGAAGCCATCTGATGTTCAGAAATATACAAAAGTCTTTATGGAAGTAGACACTGACAGAGATGGGAAAATCACTGGAGAGCAGGCACGAAATCTATTTCTGAGCTGGAGGTTACCAAGAGAGGTTCTAAAGCAGGTGTGGGACTTATCTGATCAGGATAATGACAGCATGCTTTCCTTGAGAGAGTTCTGCTTTTCTCTCTATTTGATGGAACGTTACAGGGAAGGTCGGCCTCTTCCAGCTACACTTCCAAGCAATGTTATGTTGGATGAGACACTACTGTCAATGACAGGTCAACCAAAAGTGGGTTATGGAAATGCAGCTTGGAGTCCCCACCCTGGCTTTGGACAACATCAAGGGATGCAAGGTTCCCAGATGATGCCACCTGGCACTGGTTTGAGGCCACCAATTCAGGGAAACGCCCCTCAGGCTGATCGTACAGTGCAACCGAATCAGCAAAATTTGAGGGTGCCAGGGATGGAGGGCTTGGCTCAAAACCAGCTTGACAATGGGAAGCAGGATTCAGCCAACTCAAGACCTCAAGACCCTAACAGTGCAGGGAAAGAGGCAAGTTCCTTTGTTGAAGAAACTGAGAATGTGATTTTGGACTCGAGAGAGAAGATTGAGTTCTATCGCACGAAAATGCAGGAACTTGTTCTGTATAAAAGCAGATGTGATAACAGATTAAATGAGATCACAGAAAGGGCACTGGCTGACAAGCGTGAGGCTGAACTACTGGGTAAGAAGTATGAAGAGAAATATAAACAAGTGGCAGAAATAGCATCTAAGTTAACCATTGAGGAGGCCACGTTTCGTGAAGTTCAGGAGAGGAAGATGGAATTGCATCAAGCAATTGTTAAAATGGAGCAAGGAGGTAGTGCTGATGGTATCCTTCAGGTCCGAGCTGATCGTATACAATATGATCTTGAGGAGCTAATAAAGGCTCTTACTGAACGTTGCAAGAAACATGGGCTAGACATGAAGTCAGCTGCAATAATTGAGCTTCCAACTGGTTGGCAACCTGGAATTCAAGACGGAGCAGCTGTTTGGGATGAAGATTGGGACAAGTTCGAAGATGAAGGATTTGGCAATGATCTCATAATTGATTCCTCAACAAAATCTGTCCAGAGAGAAAAGGCTTCCCCAGATCGTAGTTCAACTGCTGATTCATCATCGGTTACTGATGGAAAGTCAGGAATTTCTTACAGCAATGGTGATCATGCACATGAAAGTGAATCTGTTTTCACCCACGGTGAAGATGAACATGTAAGAAGTCCCAATGGCAGTCTAGCTGGAAGGACTGCTGTAGACAGCCCGTCTCGAGACTTCTCAGACATCCACTATGGTAAAAATTCCGAAGCAGATGGAGAAACACACGGAAGTTTTGATGAATCGACCTGGGGTGCCTTTGACAATAATGATGATATTGATTCTGTTTGGGGATTTAATACTGACAAGGGCAAGGGCACAGATTCTGAGAAGCACAGAGATTTCTTTGGATCTGATGACTATGGTGTCAACCCAGTAAGAACTGGATCCCCCAATGCAGATACCGCCTTTCAGAAGAAGAGCATTTTCTTTGAAGACTCTGTTCCTAGCACACCAGTCTCGAGGTTTGCCAACTCGCCAAGATATAGTGAGGCCGGGGATCACTACTTTGACAATTTCTCAAGGTTTGATTCATTCAGCAGCAGGCAAGACAGTGGTTTTTCTTCTCAACCGGAGAGGTTCACAAGGTTTGATTCCATGAATAGCACTAGAGATTTCGGCCACTCAAGGTTTGATTCCATAAGCAGCAGCAAAGACTTTGGCCAAAGTCATGGCCACACAAGGTTTGATTCCATAAGCAGCAGCAAAGACTTTGGCCAAAGTCATGGCCACACAAGGTTCGATTCCATAAGCAGCACAAAAGATTTTGGCCAGAGTACATACTCTTTCGATGACACAGATCCATTTGGCTCTTCTGGCCCCTTTAAGGTCACATCAGAGAGTCAAAATCCAAAGAAGGGATCTGATAATTGGAATGCTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000147 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005938 GO:0006810 GO:0006886 GO:0006897 GO:0006996 GO:0007010 GO:0008104 GO:0008150 GO:0009987 GO:0015031 GO:0015629 GO:0015833 GO:0016043 GO:0016192 GO:0022607 GO:0030029 GO:0030036 GO:0030479 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0033036 GO:0034613 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044396 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045184 GO:0046872 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061645 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0098657 GO:0099568

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

EF-hand_4 EF-hand_5

Protein Analysis

1037

Amino Acids

112.26

Weight (kDa)

5.27

Isoelectric Point (pI)

49.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EH	PF12763	21 - 76	2.9e-07	EH domain
EH	PF12763	362 - 444	4.1e-12	EH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007032)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20760
fragaria_vesca	FvH4_5g25580 FvH4_5g25580
malus_domestica	MD06G1007400.v1.1
prunus_persica	Prupe.5G009300_v2.0.a1 Prupe.5G009300_v2.0.a1
pyrus_communis	pycom06g00650 pycom06g00660
rosa_chinensis	RchiOBHm_Chr7g0215891 RchiOBHm_Chr7g0219801 RchiOBHm_Chr7g0219941
rosa_laevigata	RLG00000002612
rosa_multiflora	Rmu_co8383945.1_g000001 Rmu_sc0006240.1_g000011
rosa_roxburghii	Rroxscaffold_3G00243830
rosa_rugosa	Rorug07G0156400 Rorug07G0156500
rosa_samantha	Rh7AG294500 Rh7AG298200 Rh7BG286300 Rh7CG314500 Rh7CG318200 Rh7CG318300 Rh7DG297400
rosa_wichuraiana	Rw7G025020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	195
AarI	CACCTGC	3 cut(s)	364, 1027, 1204
AasI	GACNNNNNNGTC	2 cut(s)	1360, 2594
Acc36I	ACCTGC	4 cut(s)	364, 422, 1027, 1204
AccB1I	GGYRCC	4 cut(s)	107, 961, 1549, 2483
AccBSI	CCGCTC	1 cut(s)	50
AccI	GTMKAC	3 cut(s)	1128, 1993, 2388
AciI	CCGC	9 cut(s)	5, 50, 78, 152, 261, 952, 980, 995, 2634
AclI	AACGTT	2 cut(s)	1294, 2035
AclWI	GGATC	9 cut(s)	30, 80, 162, 2587, 2610, 2623, 2736, 3023, 3097
AcsI	RAATTY	7 cut(s)	188, 288, 1057, 1540, 2106, 2277, 2431
AcuI	CTGAAG	1 cut(s)	1958
AcyI	GRCGYC	2 cut(s)	108, 380
AdeI	CACNNNGTG	1 cut(s)	914
AfaI	GTAC	3 cut(s)	17, 1519, 3007
AflIII	ACRYGT	1 cut(s)	2344
AgsI	TTSAA	5 cut(s)	867, 1664, 2111, 2238, 2663
AhdI	GACNNNNNGTC	2 cut(s)	2062, 2395
AhlI	ACTAGT	1 cut(s)	512
AjuI	GAANNNNNNNTTGG	6 cut(s)	740, 772, 1387, 1419, 1667, 1699
AloI	GAACNNNNNNTCC	2 cut(s)	1432, 1464
Alw21I	GWGCWC	5 cut(s)	355, 424, 610, 808, 838
Alw26I	GTCTC	5 cut(s)	472, 1346, 2397, 2403, 2692
Alw44I	GTGCAC	3 cut(s)	351, 606, 834
AlwI	GGATC	9 cut(s)	30, 80, 162, 2587, 2610, 2623, 2736, 3023, 3097
AlwNI	CAGNNNCTG	3 cut(s)	281, 785, 2555
Ama87I	CYCGRG	3 cut(s)	1690, 2400, 2689
ApaLI	GTGCAC	3 cut(s)	351, 606, 834
ApoI	RAATTY	7 cut(s)	188, 288, 1057, 1540, 2106, 2277, 2431
Asp700I	GAANNNNTTC	2 cut(s)	1734, 3107
AspLEI	GCGC	1 cut(s)	110
AspS9I	GGNCC	9 cut(s)	272, 413, 560, 620, 873, 954, 998, 1978, 3047
AsuC2I	CCSGG	1 cut(s)	2725
AsuHPI	GGTGA	5 cut(s)	776, 1008, 2306, 2317, 2345
AsuII	TTCGAA	1 cut(s)	2151
AvaI	CYCGRG	3 cut(s)	1690, 2400, 2689
AvaII	GGWCC	6 cut(s)	413, 560, 620, 873, 998, 1978
AxyI	CCTNAGG	1 cut(s)	1506
BaeGI	GKGCMC	5 cut(s)	355, 610, 838, 1789, 2549
BalI	TGGCCA	7 cut(s)	942, 1075, 2892, 2904, 2946, 2958, 3000
BamHI	GGATCC	1 cut(s)	2615
BanI	GGYRCC	4 cut(s)	107, 961, 1549, 2483
BanII	GRGCYC	2 cut(s)	424, 808
BbsI	GAAGAC	1 cut(s)	2670
Bbv12I	GWGCWC	5 cut(s)	355, 424, 610, 808, 838
BbvCI	CCTCAGC	1 cut(s)	84
BceAI	ACGGC	1 cut(s)	74
BcgI	CGANNNNNNTGC	2 cut(s)	732, 766
BciVI	GTATCC	1 cut(s)	1979
BclI	TGATCA	2 cut(s)	1231, 2296
BcnI	CCSGG	1 cut(s)	2725
BcoDI	GTCTC	5 cut(s)	472, 1346, 2397, 2403, 2692
BcuI	ACTAGT	1 cut(s)	512
BfaI	CTAG	7 cut(s)	513, 828, 885, 2054, 2369, 2676, 2838
BfmI	CTRYAG	2 cut(s)	777, 2385
BfoI	RGCGCY	1 cut(s)	111
BfuAI	ACCTGC	4 cut(s)	364, 422, 1027, 1204
BfuI	GTATCC	1 cut(s)	1979
BglI	GCCNNNNNGGC	2 cut(s)	1072, 1508
Bme18I	GGWCC	6 cut(s)	413, 560, 620, 873, 998, 1978
BmeRI	GACNNNNNGTC	2 cut(s)	2062, 2395
BmeT110I	CYCGRG	3 cut(s)	1690, 2400, 2689
BmgT120I	GGNCC	9 cut(s)	272, 413, 560, 620, 873, 954, 998, 1978, 3047
BmrI	ACTGGG	2 cut(s)	1826, 2597
BmsI	GCATC	8 cut(s)	135, 495, 752, 1022, 1431, 1449, 1871, 1933
BmuI	ACTGGG	2 cut(s)	1826, 2597
BpiI	GAAGAC	1 cut(s)	2670
BplI	GAGNNNNNCTC	2 cut(s)	469, 501
BpmI	CTGGAG	3 cut(s)	657, 1176, 1207
Bpu10I	CCTNAGC	1 cut(s)	84
Bpu14I	TTCGAA	1 cut(s)	2151
BpuEI	CTTGAG	9 cut(s)	315, 471, 958, 1279, 1597, 1605, 2027, 2734, 2839
BpuMI	CCSGG	1 cut(s)	2725
BsaHI	GRCGYC	2 cut(s)	108, 380
BsaI	GGTCTC	1 cut(s)	472
BsaWI	WCCGGW	1 cut(s)	2800
BsaXI	ACNNNNNCTCC	4 cut(s)	1874, 1904, 2109, 2139
Bse118I	RCCGGY	1 cut(s)	8
Bse21I	CCTNAGG	1 cut(s)	1506
Bse3DI	GCAATG	3 cut(s)	1342, 2176, 2296
BseGI	GGATG	8 cut(s)	150, 510, 743, 1354, 1446, 1563, 2137, 2415
BseMI	GCAATG	3 cut(s)	1342, 2176, 2296
BseMII	CTCAG	9 cut(s)	75, 149, 774, 902, 1172, 1520, 1662, 2424, 2547
BseRI	GAGGAG	3 cut(s)	474, 1895, 2024
BseSI	GKGCMC	5 cut(s)	355, 610, 838, 1789, 2549
BseX3I	CGGCCG	1 cut(s)	6
BsgI	GTGCAG	2 cut(s)	309, 1656
Bsh1285I	CGRYCG	1 cut(s)	9
BshNI	GGYRCC	4 cut(s)	107, 961, 1549, 2483
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	5 cut(s)	355, 424, 610, 808, 838
BsiHKCI	CYCGRG	3 cut(s)	1690, 2400, 2689
BsiSI	CCGG	3 cut(s)	9, 2724, 2801
BslFI	GGGAC	7 cut(s)	391, 546, 859, 1235, 1395, 2156, 2340
BsmAI	GTCTC	5 cut(s)	472, 1346, 2397, 2403, 2692
BsmBI	CGTCTC	1 cut(s)	2403
BsmFI	GGGAC	7 cut(s)	391, 546, 859, 1235, 1395, 2156, 2340
BsmI	GAATGC	2 cut(s)	917, 3109
Bso31I	GGTCTC	1 cut(s)	472
BsoBI	CYCGRG	3 cut(s)	1690, 2400, 2689
Bsp119I	TTCGAA	1 cut(s)	2151
Bsp1286I	GDGCHC	7 cut(s)	355, 424, 610, 808, 838, 1789, 2549
BspACI	CCGC	9 cut(s)	5, 50, 78, 152, 261, 952, 980, 995, 2634
BspCNI	CTCAG	9 cut(s)	76, 148, 773, 903, 1173, 1519, 1663, 2423, 2548
BspMAI	CTGCAG	1 cut(s)	781
BspMI	ACCTGC	4 cut(s)	364, 422, 1027, 1204
BspPI	GGATC	9 cut(s)	30, 80, 162, 2587, 2610, 2623, 2736, 3023, 3097
BspQI	GCTCTTC	2 cut(s)	2642, 3046
BspT104I	TTCGAA	1 cut(s)	2151
BspT107I	GGYRCC	4 cut(s)	107, 961, 1549, 2483
BspTNI	GGTCTC	1 cut(s)	472
BsrBI	CCGCTC	1 cut(s)	50
BsrDI	GCAATG	3 cut(s)	1342, 2176, 2296
BsrFI	RCCGGY	1 cut(s)	8
BssAI	RCCGGY	1 cut(s)	8
BssNAI	GTATAC	1 cut(s)	1994
BssNI	GRCGYC	2 cut(s)	108, 380
BssT1I	CCWWGG	2 cut(s)	175, 1070
Bst1107I	GTATAC	1 cut(s)	1994
Bst4CI	ACNGT	8 cut(s)	217, 700, 966, 1362, 1522, 1634, 2333, 2783
Bst6I	CTCTTC	4 cut(s)	1320, 1827, 2642, 3046
BstACI	GRCGYC	2 cut(s)	108, 380
BstAPI	GCANNNNNTGC	2 cut(s)	785, 1019
BstBI	TTCGAA	1 cut(s)	2151
BstC8I	GCNNGC	6 cut(s)	150, 276, 980, 1164, 1250, 2774
BstDSI	CCRYGG	1 cut(s)	2329
BstEII	GGTNACC	1 cut(s)	1191
BstENI	CCTNNNNNAGG	2 cut(s)	429, 486
BstF5I	GGATG	8 cut(s)	150, 510, 743, 1354, 1446, 1563, 2137, 2415
BstH2I	RGCGCY	1 cut(s)	111
BstHHI	GCGC	1 cut(s)	110
BstMAI	GTCTC	5 cut(s)	472, 1346, 2397, 2403, 2692
BstMCI	CGRYCG	1 cut(s)	9
BstNSI	RCATGY	3 cut(s)	152, 1252, 2348
BstPI	GGTNACC	1 cut(s)	1191
BstSFI	CTRYAG	2 cut(s)	777, 2385
BstSLI	GKGCMC	5 cut(s)	355, 610, 838, 1789, 2549
BstV2I	GAAGAC	1 cut(s)	2670
BstX2I	RGATCY	4 cut(s)	2579, 2615, 3028, 3089
BstXI	CCANNNNNNTGG	3 cut(s)	2094, 2900, 2954
BstYI	RGATCY	4 cut(s)	2579, 2615, 3028, 3089
BstZ17I	GTATAC	1 cut(s)	1994
BstZI	CGGCCG	1 cut(s)	6
Bsu36I	CCTNAGG	1 cut(s)	1506
BsuI	GTATCC	1 cut(s)	1979
BtgI	CCRYGG	1 cut(s)	2329
BtsCI	GGATG	8 cut(s)	150, 510, 743, 1354, 1446, 1563, 2137, 2415
BveI	ACCTGC	4 cut(s)	364, 422, 1027, 1204
Cac8I	GCNNGC	6 cut(s)	150, 276, 980, 1164, 1250, 2774
CaiI	CAGNNNCTG	3 cut(s)	281, 785, 2555
CfoI	GCGC	1 cut(s)	110
Cfr10I	RCCGGY	1 cut(s)	8
Cfr13I	GGNCC	9 cut(s)	272, 413, 560, 620, 873, 954, 998, 1978, 3047
CseI	GACGC	1 cut(s)	388
Csp6I	GTAC	3 cut(s)	16, 1518, 3006
CviQI	GTAC	3 cut(s)	16, 1518, 3006
DinI	GGCGCC	1 cut(s)	109
DraIII	CACNNNGTG	1 cut(s)	914
DrdI	GACNNNNNNGTC	2 cut(s)	1360, 2594
DriI	GACNNNNNGTC	2 cut(s)	2062, 2395
DseDI	GACNNNNNNGTC	2 cut(s)	1360, 2594
EagI	CGGCCG	1 cut(s)	6
Eam1104I	CTCTTC	4 cut(s)	1320, 1827, 2642, 3046
Eam1105I	GACNNNNNGTC	2 cut(s)	2062, 2395
EarI	CTCTTC	4 cut(s)	1320, 1827, 2642, 3046
Ecl136II	GAGCTC	2 cut(s)	422, 806
EclXI	CGGCCG	1 cut(s)	6
Eco130I	CCWWGG	2 cut(s)	175, 1070
Eco24I	GRGCYC	2 cut(s)	424, 808
Eco31I	GGTCTC	1 cut(s)	472
Eco47I	GGWCC	6 cut(s)	413, 560, 620, 873, 998, 1978
Eco52I	CGGCCG	1 cut(s)	6
Eco53kI	GAGCTC	2 cut(s)	422, 806
Eco57I	CTGAAG	1 cut(s)	1958
Eco81I	CCTNAGG	1 cut(s)	1506
Eco88I	CYCGRG	3 cut(s)	1690, 2400, 2689
Eco91I	GGTNACC	1 cut(s)	1191
EcoICRI	GAGCTC	2 cut(s)	422, 806
EcoNI	CCTNNNNNAGG	2 cut(s)	429, 486
EcoO109I	RGGNCCY	3 cut(s)	560, 873, 998
EcoO65I	GGTNACC	1 cut(s)	1191
EcoRI	GAATTC	1 cut(s)	2106
EcoT14I	CCWWGG	2 cut(s)	175, 1070
EcoT22I	ATGCAT	1 cut(s)	150
EcoT38I	GRGCYC	2 cut(s)	424, 808
EgeI	GGCGCC	1 cut(s)	109
EheI	GGCGCC	1 cut(s)	109
ErhI	CCWWGG	2 cut(s)	175, 1070
Esp3I	CGTCTC	1 cut(s)	2403
FaqI	GGGAC	7 cut(s)	391, 546, 859, 1235, 1395, 2156, 2340
FauI	CCCGC	2 cut(s)	987, 988
FbaI	TGATCA	2 cut(s)	1231, 2296
FblI	GTMKAC	3 cut(s)	1128, 1993, 2388
FokI	GGATG	8 cut(s)	157, 517, 730, 1361, 1453, 1570, 2144, 2402
FriOI	GRGCYC	2 cut(s)	424, 808
FspBI	CTAG	7 cut(s)	513, 828, 885, 2054, 2369, 2676, 2838
GlaI	GCGC	1 cut(s)	109
GsuI	CTGGAG	3 cut(s)	657, 1176, 1207
HaeII	RGCGCY	1 cut(s)	111
HapII	CCGG	3 cut(s)	9, 2724, 2801
HgaI	GACGC	1 cut(s)	388
HhaI	GCGC	1 cut(s)	110
Hin1I	GRCGYC	2 cut(s)	108, 380
Hin6I	GCGC	1 cut(s)	108
HinP1I	GCGC	1 cut(s)	108
HincII	GTYRAC	5 cut(s)	681, 816, 1376, 1872, 2599
HindII	GTYRAC	5 cut(s)	681, 816, 1376, 1872, 2599
HpaI	GTTAAC	1 cut(s)	1872
HpaII	CCGG	3 cut(s)	9, 2724, 2801
HphI	GGTGA	5 cut(s)	776, 1008, 2306, 2317, 2345
Hpy99I	CGWCG	1 cut(s)	95
HpyCH4III	ACNGT	8 cut(s)	217, 700, 966, 1362, 1522, 1634, 2333, 2783
HpyCH4IV	ACGT	3 cut(s)	1294, 1889, 2035
HpySE526I	ACGT	3 cut(s)	1294, 1889, 2035
Hsp92I	GRCGYC	2 cut(s)	108, 380
HspAI	GCGC	1 cut(s)	108
KasI	GGCGCC	1 cut(s)	107
KflI	GGGWCCC	2 cut(s)	560, 873
Ksp22I	TGATCA	2 cut(s)	1231, 2296
KspAI	GTTAAC	1 cut(s)	1872
LguI	GCTCTTC	2 cut(s)	2642, 3046
LweI	GCATC	8 cut(s)	135, 495, 752, 1022, 1431, 1449, 1871, 1933
MaeI	CTAG	7 cut(s)	513, 828, 885, 2054, 2369, 2676, 2838
MaeII	ACGT	3 cut(s)	1294, 1889, 2035
MaeIII	GTNAC	5 cut(s)	211, 1191, 1295, 2257, 3058
MbiI	CCGCTC	1 cut(s)	50
MfeI	CAATTG	1 cut(s)	1932
MflI	RGATCY	4 cut(s)	2579, 2615, 3028, 3089
MhlI	GDGCHC	7 cut(s)	355, 424, 610, 808, 838, 1789, 2549
MlsI	TGGCCA	7 cut(s)	942, 1075, 2892, 2904, 2946, 2958, 3000
MluNI	TGGCCA	7 cut(s)	942, 1075, 2892, 2904, 2946, 2958, 3000
Mly113I	GGCGCC	1 cut(s)	108
MlyI	GAGTC	6 cut(s)	701, 1044, 1417, 1682, 2660, 3079
MmeI	TCCRAC	4 cut(s)	1012, 1018, 1326, 2111
Mox20I	TGGCCA	7 cut(s)	942, 1075, 2892, 2904, 2946, 2958, 3000
Mph1103I	ATGCAT	1 cut(s)	150
MroXI	GAANNNNTTC	2 cut(s)	1734, 3107
MscI	TGGCCA	7 cut(s)	942, 1075, 2892, 2904, 2946, 2958, 3000
MseI	TTAA	6 cut(s)	239, 1766, 1871, 1938, 2526, 3054
MslI	CAYNNNNRTG	3 cut(s)	1721, 2304, 2310
Msp20I	TGGCCA	7 cut(s)	942, 1075, 2892, 2904, 2946, 2958, 3000
MspA1I	CMGCKG	7 cut(s)	28, 78, 278, 952, 992, 2069, 2123
MspI	CCGG	3 cut(s)	9, 2724, 2801
MunI	CAATTG	1 cut(s)	1932
Mva1269I	GAATGC	2 cut(s)	917, 3109
NarI	GGCGCC	1 cut(s)	108
NciI	CCSGG	1 cut(s)	2725
NmuCI	GTSAC	2 cut(s)	211, 3058
NsiI	ATGCAT	1 cut(s)	150
NspI	RCATGY	3 cut(s)	152, 1252, 2348
NspV	TTCGAA	1 cut(s)	2151
PaeI	GCATGC	2 cut(s)	152, 1252
PaeR7I	CTCGAG	3 cut(s)	1690, 2400, 2689
PaqCI	CACCTGC	3 cut(s)	364, 1027, 1204
PasI	CCCWGGG	1 cut(s)	500
PciI	ACATGT	1 cut(s)	2344
PciSI	GCTCTTC	2 cut(s)	2642, 3046
PctI	GAATGC	2 cut(s)	917, 3109
PdmI	GAANNNNTTC	2 cut(s)	1734, 3107
PleI	GAGTC	6 cut(s)	701, 1043, 1416, 1682, 2660, 3078
PluTI	GGCGCC	1 cut(s)	111
PpsI	GAGTC	6 cut(s)	701, 1043, 1416, 1682, 2660, 3078
PpuMI	RGGWCCY	3 cut(s)	560, 873, 998
PscI	ACATGT	1 cut(s)	2344
PsiI	TTATAA	1 cut(s)	195
Psp124BI	GAGCTC	2 cut(s)	424, 808
Psp1406I	AACGTT	2 cut(s)	1294, 2035
Psp5II	RGGWCCY	3 cut(s)	560, 873, 998
PspEI	GGTNACC	1 cut(s)	1191
PspPI	GGNCC	9 cut(s)	272, 413, 560, 620, 873, 954, 998, 1978, 3047
PspPPI	RGGWCCY	3 cut(s)	560, 873, 998
PstI	CTGCAG	1 cut(s)	781
PstNI	CAGNNNCTG	3 cut(s)	281, 785, 2555
PsuI	RGATCY	4 cut(s)	2579, 2615, 3028, 3089
PvuII	CAGCTG	5 cut(s)	28, 278, 992, 2069, 2123
RsaI	GTAC	3 cut(s)	17, 1519, 3007
RsaNI	GTAC	3 cut(s)	16, 1518, 3006
RseI	CAYNNNNRTG	3 cut(s)	1721, 2304, 2310
SacI	GAGCTC	2 cut(s)	424, 808
SapI	GCTCTTC	2 cut(s)	2642, 3046
SaqAI	TTAA	6 cut(s)	239, 1766, 1871, 1938, 2526, 3054
Sau96I	GGNCC	9 cut(s)	272, 413, 560, 620, 873, 954, 998, 1978, 3047
SchI	GAGTC	6 cut(s)	701, 1044, 1417, 1682, 2660, 3079
SduI	GDGCHC	7 cut(s)	355, 424, 610, 808, 838, 1789, 2549
SfaNI	GCATC	8 cut(s)	135, 495, 752, 1022, 1431, 1449, 1871, 1933
SfcI	CTRYAG	2 cut(s)	777, 2385
SfoI	GGCGCC	1 cut(s)	109
Sfr274I	CTCGAG	3 cut(s)	1690, 2400, 2689
SfuI	TTCGAA	1 cut(s)	2151
SinI	GGWCC	6 cut(s)	413, 560, 620, 873, 998, 1978
SlaI	CTCGAG	3 cut(s)	1690, 2400, 2689
SmiMI	CAYNNNNRTG	3 cut(s)	1721, 2304, 2310
SpeI	ACTAGT	1 cut(s)	512
SphI	GCATGC	2 cut(s)	152, 1252
SsiI	CCGC	9 cut(s)	5, 50, 78, 152, 261, 952, 980, 995, 2634
SspDI	GGCGCC	1 cut(s)	107
SspMI	CTAG	7 cut(s)	513, 828, 885, 2054, 2369, 2676, 2838
SstI	GAGCTC	2 cut(s)	424, 808
StyI	CCWWGG	2 cut(s)	175, 1070
TaaI	ACNGT	8 cut(s)	217, 700, 966, 1362, 1522, 1634, 2333, 2783
TaiI	ACGT	3 cut(s)	1297, 1892, 2038
TaqI	TCGA	7 cut(s)	1691, 2151, 2401, 2474, 2690, 2970, 3018
TatI	WGTACW	1 cut(s)	3005
TauI	GCSGC	3 cut(s)	8, 263, 955
Tru1I	TTAA	6 cut(s)	239, 1766, 1871, 1938, 2526, 3054
Tru9I	TTAA	6 cut(s)	239, 1766, 1871, 1938, 2526, 3054
TseFI	GTSAC	2 cut(s)	211, 3058
Tsp45I	GTSAC	2 cut(s)	211, 3058
TspGWI	ACGGA	4 cut(s)	35, 75, 2130, 2472
VneI	GTGCAC	3 cut(s)	351, 606, 834
VpaK11BI	GGWCC	6 cut(s)	413, 560, 620, 873, 998, 1978
XagI	CCTNNNNNAGG	2 cut(s)	429, 486
XapI	RAATTY	7 cut(s)	188, 288, 1057, 1540, 2106, 2277, 2431
XceI	RCATGY	3 cut(s)	152, 1252, 2348
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1421, 1586
XhoI	CTCGAG	3 cut(s)	1690, 2400, 2689
XmiI	GTMKAC	3 cut(s)	1128, 1993, 2388
XmnI	GAANNNNTTC	2 cut(s)	1734, 3107
XspI	CTAG	7 cut(s)	513, 828, 885, 2054, 2369, 2676, 2838
Zsp2I	ATGCAT	1 cut(s)	150

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.