Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0007032

Physical Location & Seq

Genomic Coordinates Reverse (-)

741935 .. 749888

Gene Structure 7954 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1007400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3087 bp

ATGGCGGGTCCGTATGCGGATCAGCTTGAAGCCTACTTCAGGAGAGCAGATTTGGATGGAGATGGCAGGATCAGCGGAGCTGAAGCCGTCGCTTTCTTCCAGGGTTCCAATTTGCCCAAACCAGTTCTTGCTCAGATATGGATGCATGCTGATCAGAACAAAACTGGTTTTCTTGGTCGGCCAGAATTTTATAATGCACTCAGACTTGTGACCGTTGCACAGAGTAAGAGAGATTTAACTCCGGATATTGTCAAGGCAGCATTATACGGTCCAGCTGCTGCAAAAATTCCTGCTCCACAGATTAATCTTCCACCCACATCTGCACCTCAATCAAATCCAGTGGCTGCTGCATCTAGGCCACAGATGGGTATGGGAACCCTGCCAACATCTCAAAATTTTGGATTTAGAGGACAAGGAGTTCCTAATGCATCCACGAACCAGAATTATTTTCCACCCCAGCAAAATCAGTCCATGAGACCTCCTCAAGCTCTGCCGACTGGAATGCCTACTGGCGTTAATTCTCGTCCACCCCAACAAGGTCTTGGAGGTGGTATGGGGCCTCCCAATGTTCCAAACTCAAACATCTCAAATAATTGGCTTGGTGGAAGCGCTGGTGCATCTCCTGCTGGGCCCAGAGGGGTTAGTCCTTCCATGCCCTCATCTACACCAAACTCACAGCCTCCAGTTTCCATGTCTTCCCTGCCAACAACTGGCGACTCCAAACCATTAGTTGTTTCTGGCAATGGATTTGCATCCAGTTCGGCTTTATCAGGTGATTTATTTTCTGCAACTCCCAGTCAACCGAAACAGGAATCTTCTGGGTCTATATATTCTGCTAGAAGCATACCTACCCCATCAGCCACTCTTCCAGTTTCCAGTGGGTCCCAATCTTCCAGTAAGCTCAATGCGCTTGATTCATTGAGTGCATTCACCATGCAGCCTTCTGGCAGTCAGTTTCAGCGGCCGCAGGGACCTTCGAACCCAAGCCAGCAGGTTTCAGCTCCAGCTTCTTCCTCTTTTCCATCATCTGGGAGTTCAGTGGGAGCAGGAAACTCTACGTCCGGAAATTCGCAGATTCCTTGGCCAAAGATGAAGCCATCTGATGTCCAGAAATATACAAAAGTGTTTATGGAAGTAGACACTGACAGAGATGGGAGAATCACTGGTGAGCAGGCACGGAATCTGTTTCTGAGTTGGAGGTTACCAAGAGAGGTTTTAAAGCAGGTGTGGGACTTATCTGATCAAGATAATGACAGCATGCTTTCCTTGAGGGAGTTCTGCTTTTCTCTCTATTTGATGGAACGTTACAGGGAAGGTCGTCCTCTTCCAGACACACTTCCACATAATGTGATGCATGATGAGACTCTACTCTCAATGACCGGTCAACCCAAAGTGGCTTATGGAAATGCAGCTTGGAGTCCCAACCCTGGTTTTGGACAACATCAAGGGATGCAGGGTGTGGCACCTGCTGCGGGTTTGAGGCCACCAATGCAACGAAGTCTTCCCCAGGCTGACAGTGCATTGCAACCGAATCAGCAGAATTTGAGAGTGCGAGGGATGGAGGGTTTGAATACAACCCAACATGATAATGGGAAGCATGATTCAGCTAACTCAAATCCTGAAGAGCCTAAAGATGCAGGGAAAAAGGTTGAAGAAACTGAGAATGTGATTTTGGACTCAAGAGAGAAGATGGAGTTCTATCGCACAAAAATGCAGGAACTCGTTCTGTATAAAAGCAGATGTGATAACAGATTAAATGAGATCACAGAAAGGGCAATTGCTGACAAGCGTGAGGCTGAGTTACTGGCCAAAAAATATGAGGAGAAGTATAAACAAGTGGCAGAAATAGCATCTAAGTTAACTATTGAAGAGGCCACGTTTCGCGAAGTTCAGGAGAGGAAAATGGAATTGCATCAAGCAATTGTTAAAATGGAGCAAGGAGGTAGTGCAGATGGTATTCTTCAGGTCCGTGCTGATCGTATACAATATGATCTTGAGGAGCTAGTAAAGGCTCTTACAGAACGTTGCAAGAAACATGGATTAAACATGAAGTCAAGTGCAATAATTGAGCTTCCAACTGGCTGGCAACCTGGAATTCAAGAGGGAGCCGCTGTTTGGGATGAAGATTGGGATAAGTTTGAAGATGAAGGATTTGGTAATGATCTGACAATTGATGCCTCGGCAAAAGCACAATCTGCATCTGTTCAGAGGGAACATACTTCCCCTGATCCTAGTTCCACTCCTGATACATCATCATTTGCTGATCGCAAGTCAAGGAATGGGGAACATGCCTTTGGAAGTGAATCTGTTTTCGCCCATGGTGAAGATGAATATGGAAGAAGCTCAAATGGCAGTCCAGCTGCCAGGAATGCTCCAGAAAGCCCGTCAGGAGAGTTCTCTGACATCCATTATGGTAAAAGTTCTGAAGCAGATGCAGAAACACATGGTTTTGATGAATCGACCTGGGGTGGCGCCTTTGACAATAATGATGATACTGACTCCGTCTGGGGTTTTAACACCAAGGGCTCAGATGCTGAGAAGCATAAAGATTTCTTTGGATCGGACGACTTTGGTCTCAACCCAATAAGAACTGGTTCCCCCCACGCGGAAACCAGCTTTCAGAAAAAGAGCCTGTTCTTTGAGGACTCTGTTCCCAGCACTCCGCTCTCCAAATTTCAGAACTCTCCAAGATACAGCGAGGCTGGGGACTACCACTTTGACAATTTATCAAGGTTTGATTCCTTCAGCAGCAACAGGCATGACGTTGGCTTTTCTTCCCAACCTGAGAGGTTCTCGAGGTTCGATTCCATAAATAGCACTAGAGATTTTGGGGGCCACACAAGGTTTGACTCCATAAGCAGCAGCAAGGACTTTGGTCGGTTTGACTCCATGAGCAGCAGCAGGGACTTTGGTCAGGGTCGCGAGCCGCAGCTCACAAGGTTCGACTCCATAAACAGCACCAAAGATTTCGGCCAGGGTGCATATTCTTTCGACGAGACTGATCCATTCGGCTCTTCTGGGCCATTTAAGGTCTCATCAGAGAGTCAAACATCGAAGAAAGGATCTGATAGCTGGAGCGCTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000147 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005938 GO:0006810 GO:0006886 GO:0006897 GO:0006996 GO:0007010 GO:0008104 GO:0008150 GO:0009987 GO:0015031 GO:0015629 GO:0015833 GO:0016043 GO:0016192 GO:0022607 GO:0030029 GO:0030036 GO:0030479 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0033036 GO:0034613 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044396 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045184 GO:0046872 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061645 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0098657 GO:0099568

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

EF-hand_4 EF-hand_5

Protein Analysis

1029

Amino Acids

111.72

Weight (kDa)

5.36

Isoelectric Point (pI)

52.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EH	PF12763	20 - 75	4.1e-08	EH domain
EH	PF12763	365 - 449	1.2e-11	EH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007032)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20760
fragaria_vesca	FvH4_5g25580 FvH4_5g25580
malus_domestica	MD06G1007400.v1.1
prunus_persica	Prupe.5G009300_v2.0.a1 Prupe.5G009300_v2.0.a1
pyrus_communis	pycom06g00650 pycom06g00660
rosa_chinensis	RchiOBHm_Chr7g0215891 RchiOBHm_Chr7g0219801 RchiOBHm_Chr7g0219941
rosa_laevigata	RLG00000002612
rosa_multiflora	Rmu_co8383945.1_g000001 Rmu_sc0006240.1_g000011
rosa_roxburghii	Rroxscaffold_3G00243830
rosa_rugosa	Rorug07G0156400 Rorug07G0156500
rosa_samantha	Rh7AG294500 Rh7AG298200 Rh7BG286300 Rh7CG314500 Rh7CG318200 Rh7CG318300 Rh7DG297400
rosa_wichuraiana	Rw7G025020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	192
AarI	CACCTGC	2 cut(s)	1213, 1474
Acc36I	ACCTGC	3 cut(s)	982, 1213, 1474
AccB1I	GGYRCC	2 cut(s)	1462, 2471
AccB7I	CCANNNNNTGG	2 cut(s)	710, 1028
AccBSI	CCGCTC	1 cut(s)	2665
AccI	GTMKAC	2 cut(s)	1137, 1981
AccII	CGCG	3 cut(s)	1884, 2606, 2922
AccIII	TCCGGA	2 cut(s)	241, 1061
AclI	AACGTT	2 cut(s)	1303, 2023
AclWI	GGATC	6 cut(s)	27, 77, 2222, 2566, 2996, 3070
AcoI	YGGCCR	5 cut(s)	179, 962, 1082, 1806, 2971
AcsI	RAATTY	7 cut(s)	185, 285, 394, 1066, 1540, 2094, 2672
AcuI	CTGAAG	6 cut(s)	22, 102, 1641, 1946, 2445, 2728
AcyI	GRCGYC	1 cut(s)	2472
AfeI	AGCGCT	2 cut(s)	610, 3079
AgeI	ACCGGT	1 cut(s)	1379
AgsI	TTSAA	6 cut(s)	29, 1570, 1652, 1868, 2099, 2141
AjnI	CCWGG	7 cut(s)	99, 1426, 1506, 2089, 2363, 2462, 2973
AjuI	GAANNNNNNNTTGG	6 cut(s)	1396, 1428, 1655, 1687, 2277, 2309
AloI	GAACNNNNNNTCC	2 cut(s)	402, 434
Alw26I	GTCTC	5 cut(s)	469, 1355, 2579, 2990, 3037
AlwI	GGATC	6 cut(s)	27, 77, 2222, 2566, 2996, 3070
AlwNI	CAGNNNCTG	3 cut(s)	278, 344, 2534
Ama87I	CYCGRG	1 cut(s)	2794
Aor13HI	TCCGGA	2 cut(s)	241, 1061
Aor51HI	AGCGCT	2 cut(s)	610, 3079
ApaI	GGGCCC	1 cut(s)	633
ApoI	RAATTY	7 cut(s)	185, 285, 394, 1066, 1540, 2094, 2672
ArsI	GACNNNNNNTTYG	2 cut(s)	2699, 2731
AseI	ATTAAT	1 cut(s)	303
AsiGI	ACCGGT	1 cut(s)	1379
Asp700I	GAANNNNTTC	2 cut(s)	1722, 2593
AspLEI	GCGC	4 cut(s)	611, 910, 2474, 3080
AsuHPI	GGTGA	4 cut(s)	785, 922, 1178, 2333
AsuII	TTCGAA	1 cut(s)	977
AvaI	CYCGRG	1 cut(s)	2794
AvaII	GGWCC	5 cut(s)	8, 269, 882, 971, 1966
BaeGI	GKGCMC	1 cut(s)	633
BalI	TGGCCA	2 cut(s)	1084, 1808
BanI	GGYRCC	2 cut(s)	1462, 2471
BanII	GRGCYC	2 cut(s)	633, 2528
BarI	GAAGNNNNNNTAC	2 cut(s)	832, 864
BbsI	GAAGAC	2 cut(s)	687, 1493
BceAI	ACGGC	1 cut(s)	71
BcgI	CGANNNNNNTGC	4 cut(s)	484, 518, 741, 775
BciT130I	CCWGG	7 cut(s)	101, 1428, 1508, 2091, 2365, 2464, 2975
BclI	TGATCA	2 cut(s)	151, 1240
BcoDI	GTCTC	5 cut(s)	469, 1355, 2579, 2990, 3037
BfaI	CTAG	5 cut(s)	354, 837, 2003, 2232, 2820
BfoI	RGCGCY	3 cut(s)	612, 2475, 3081
BfuAI	ACCTGC	3 cut(s)	982, 1213, 1474
Bme1390I	CCNGG	7 cut(s)	101, 1428, 1508, 2091, 2365, 2464, 2975
Bme18I	GGWCC	5 cut(s)	8, 269, 882, 971, 1966
BmeT110I	CYCGRG	1 cut(s)	2794
BmrFI	CCNGG	7 cut(s)	101, 1428, 1508, 2091, 2365, 2464, 2975
BmrI	ACTGGG	1 cut(s)	789
BmuI	ACTGGG	1 cut(s)	789
BoxI	GACNNNNGTC	3 cut(s)	2571, 2874, 2910
BpiI	GAAGAC	2 cut(s)	687, 1493
BplI	GAGNNNNNCTC	4 cut(s)	466, 498, 2777, 2809
BpmI	CTGGAG	3 cut(s)	666, 987, 2358
Bpu14I	TTCGAA	1 cut(s)	977
BpuEI	CTTGAG	4 cut(s)	468, 1288, 1663, 2015
BsaBI	GATNNNNATC	1 cut(s)	751
BsaHI	GRCGYC	1 cut(s)	2472
BsaI	GGTCTC	3 cut(s)	469, 2579, 3037
BsaJI	CCNNGG	9 cut(s)	100, 1079, 1426, 1506, 2178, 2317, 2463, 2520, 2974
BsaWI	WCCGGW	3 cut(s)	241, 1061, 1379
BsaXI	ACNNNNNCTCC	2 cut(s)	2097, 2127
Bse118I	RCCGGY	1 cut(s)	1379
Bse3DI	GCAATG	2 cut(s)	748, 1520
Bse8I	GATNNNNATC	1 cut(s)	751
BseAI	TCCGGA	2 cut(s)	241, 1061
BseBI	CCWGG	7 cut(s)	101, 1428, 1508, 2091, 2365, 2464, 2975
BseDI	CCNNGG	9 cut(s)	100, 1079, 1426, 1506, 2178, 2317, 2463, 2520, 2974
BseGI	GGATG	8 cut(s)	61, 147, 428, 752, 1455, 1563, 2125, 2403
BseJI	GATNNNNATC	1 cut(s)	751
BseMI	GCAATG	2 cut(s)	748, 1520
BseMII	CTCAG	8 cut(s)	146, 214, 1181, 1650, 1788, 2526, 2541, 2775
BseRI	GAGGAG	3 cut(s)	471, 1835, 2012
BseSI	GKGCMC	1 cut(s)	633
BseX3I	CGGCCG	1 cut(s)	962
BseYI	CCCAGC	4 cut(s)	456, 626, 2654, 2702
BsgI	GTGCAG	2 cut(s)	306, 1968
Bsh1236I	CGCG	3 cut(s)	1884, 2606, 2922
Bsh1285I	CGRYCG	1 cut(s)	965
BshNI	GGYRCC	2 cut(s)	1462, 2471
BshTI	ACCGGT	1 cut(s)	1379
BsiEI	CGRYCG	1 cut(s)	965
BsiHKCI	CYCGRG	1 cut(s)	2794
BsiSI	CCGG	3 cut(s)	242, 1062, 1380
BslFI	GGGAC	6 cut(s)	868, 984, 1244, 1404, 2720, 2918
BsmAI	GTCTC	5 cut(s)	469, 1355, 2579, 2990, 3037
BsmFI	GGGAC	6 cut(s)	868, 984, 1244, 1404, 2720, 2918
BsmI	GAATGC	3 cut(s)	507, 926, 2374
Bso31I	GGTCTC	3 cut(s)	469, 2579, 3037
BsoBI	CYCGRG	1 cut(s)	2794
Bsp119I	TTCGAA	1 cut(s)	977
Bsp120I	GGGCCC	1 cut(s)	629
Bsp1286I	GDGCHC	2 cut(s)	633, 2528
Bsp13I	TCCGGA	2 cut(s)	241, 1061
Bsp19I	CCATGG	1 cut(s)	2317
Bsp68I	TCGCGA	2 cut(s)	1884, 2922
BspCNI	CTCAG	8 cut(s)	145, 213, 1182, 1651, 1789, 2527, 2540, 2776
BspEI	TCCGGA	2 cut(s)	241, 1061
BspFNI	CGCG	3 cut(s)	1884, 2606, 2922
BspMI	ACCTGC	3 cut(s)	982, 1213, 1474
BspPI	GGATC	6 cut(s)	27, 77, 2222, 2566, 2996, 3070
BspQI	GCTCTTC	2 cut(s)	1617, 3019
BspT104I	TTCGAA	1 cut(s)	977
BspT107I	GGYRCC	2 cut(s)	1462, 2471
BspTNI	GGTCTC	3 cut(s)	469, 2579, 3037
BsrBI	CCGCTC	1 cut(s)	2665
BsrDI	GCAATG	2 cut(s)	748, 1520
BsrFI	RCCGGY	1 cut(s)	1379
BssAI	RCCGGY	1 cut(s)	1379
BssECI	CCNNGG	9 cut(s)	100, 1079, 1426, 1506, 2178, 2317, 2463, 2520, 2974
BssNAI	GTATAC	1 cut(s)	1982
BssNI	GRCGYC	1 cut(s)	2472
BssT1I	CCWWGG	3 cut(s)	1079, 2317, 2520
Bst1107I	GTATAC	1 cut(s)	1982
Bst2UI	CCWGG	7 cut(s)	101, 1428, 1508, 2091, 2365, 2464, 2975
Bst4CI	ACNGT	3 cut(s)	214, 269, 1517
Bst6I	CTCTTC	5 cut(s)	870, 1329, 1617, 1863, 3019
BstACI	GRCGYC	1 cut(s)	2472
BstAPI	GCANNNNNTGC	3 cut(s)	623, 1469, 2195
BstBI	TTCGAA	1 cut(s)	977
BstC8I	GCNNGC	6 cut(s)	147, 989, 1173, 1259, 2084, 2924
BstDEI	CTNAG	9 cut(s)	132, 200, 1190, 1659, 1797, 1854, 2527, 2535, 2784
BstDSI	CCRYGG	1 cut(s)	2317
BstEII	GGTNACC	1 cut(s)	1200
BstENI	CCTNNNNNAGG	1 cut(s)	37
BstF5I	GGATG	8 cut(s)	61, 147, 428, 752, 1455, 1563, 2125, 2403
BstFNI	CGCG	3 cut(s)	1884, 2606, 2922
BstH2I	RGCGCY	3 cut(s)	612, 2475, 3081
BstHHI	GCGC	4 cut(s)	611, 910, 2474, 3080
BstMAI	GTCTC	5 cut(s)	469, 1355, 2579, 2990, 3037
BstMCI	CGRYCG	1 cut(s)	965
BstNI	CCWGG	7 cut(s)	101, 1428, 1508, 2091, 2365, 2464, 2975
BstNSI	RCATGY	3 cut(s)	149, 1261, 2291
BstPAI	GACNNNNGTC	3 cut(s)	2571, 2874, 2910
BstPI	GGTNACC	1 cut(s)	1200
BstSCI	CCNGG	7 cut(s)	99, 1426, 1506, 2089, 2363, 2462, 2973
BstSLI	GKGCMC	1 cut(s)	633
BstUI	CGCG	3 cut(s)	1884, 2606, 2922
BstV2I	GAAGAC	2 cut(s)	687, 1493
BstX2I	RGATCY	1 cut(s)	3062
BstXI	CCANNNNNNTGG	1 cut(s)	2082
BstYI	RGATCY	1 cut(s)	3062
BstZ17I	GTATAC	1 cut(s)	1982
BstZI	CGGCCG	1 cut(s)	962
BtgI	CCRYGG	1 cut(s)	2317
BtsCI	GGATG	8 cut(s)	61, 147, 428, 752, 1455, 1563, 2125, 2403
BtsIMutI	CAGTG	6 cut(s)	345, 883, 1044, 1140, 1161, 1522
BtuMI	TCGCGA	2 cut(s)	1884, 2922
BveI	ACCTGC	3 cut(s)	982, 1213, 1474
Cac8I	GCNNGC	6 cut(s)	147, 989, 1173, 1259, 2084, 2924
CaiI	CAGNNNCTG	3 cut(s)	278, 344, 2534
CciNI	GCGGCCGC	1 cut(s)	962
CfoI	GCGC	4 cut(s)	611, 910, 2474, 3080
Cfr10I	RCCGGY	1 cut(s)	1379
CspAI	ACCGGT	1 cut(s)	1379
CspCI	CAANNNNNGTGG	2 cut(s)	321, 356
DdeI	CTNAG	9 cut(s)	132, 200, 1190, 1659, 1797, 1854, 2527, 2535, 2784
DinI	GGCGCC	1 cut(s)	2473
DraI	TTTAAA	1 cut(s)	1218
EaeI	YGGCCR	5 cut(s)	179, 962, 1082, 1806, 2971
EagI	CGGCCG	1 cut(s)	962
Eam1104I	CTCTTC	5 cut(s)	870, 1329, 1617, 1863, 3019
EarI	CTCTTC	5 cut(s)	870, 1329, 1617, 1863, 3019
EclXI	CGGCCG	1 cut(s)	962
Eco130I	CCWWGG	3 cut(s)	1079, 2317, 2520
Eco24I	GRGCYC	2 cut(s)	633, 2528
Eco31I	GGTCTC	3 cut(s)	469, 2579, 3037
Eco47I	GGWCC	5 cut(s)	8, 269, 882, 971, 1966
Eco47III	AGCGCT	2 cut(s)	610, 3079
Eco52I	CGGCCG	1 cut(s)	962
Eco57I	CTGAAG	6 cut(s)	22, 102, 1641, 1946, 2445, 2728
Eco88I	CYCGRG	1 cut(s)	2794
Eco91I	GGTNACC	1 cut(s)	1200
EcoNI	CCTNNNNNAGG	1 cut(s)	37
EcoO109I	RGGNCCY	3 cut(s)	557, 882, 971
EcoO65I	GGTNACC	1 cut(s)	1200
EcoRI	GAATTC	1 cut(s)	2094
EcoRII	CCWGG	7 cut(s)	99, 1426, 1506, 2089, 2363, 2462, 2973
EcoT14I	CCWWGG	3 cut(s)	1079, 2317, 2520
EcoT22I	ATGCAT	3 cut(s)	147, 430, 1356
EcoT38I	GRGCYC	2 cut(s)	633, 2528
EgeI	GGCGCC	1 cut(s)	2473
EheI	GGCGCC	1 cut(s)	2473
ErhI	CCWWGG	3 cut(s)	1079, 2317, 2520
FaqI	GGGAC	6 cut(s)	868, 984, 1244, 1404, 2720, 2918
FauI	CCCGC	1 cut(s)	1465
FbaI	TGATCA	2 cut(s)	151, 1240
FblI	GTMKAC	2 cut(s)	1137, 1981
FokI	GGATG	8 cut(s)	68, 154, 415, 739, 1462, 1570, 2132, 2390
FriOI	GRGCYC	2 cut(s)	633, 2528
FspBI	CTAG	5 cut(s)	354, 837, 2003, 2232, 2820
GlaI	GCGC	4 cut(s)	610, 909, 2473, 3079
GsaI	CCCAGC	4 cut(s)	460, 630, 2658, 2706
GsuI	CTGGAG	3 cut(s)	666, 987, 2358
HaeII	RGCGCY	3 cut(s)	612, 2475, 3081
HapII	CCGG	3 cut(s)	242, 1062, 1380
HhaI	GCGC	4 cut(s)	611, 910, 2474, 3080
Hin1I	GRCGYC	1 cut(s)	2472
Hin6I	GCGC	4 cut(s)	609, 908, 2472, 3078
HinP1I	GCGC	4 cut(s)	609, 908, 2472, 3078
HincII	GTYRAC	3 cut(s)	800, 1385, 1860
HindII	GTYRAC	3 cut(s)	800, 1385, 1860
HpaI	GTTAAC	1 cut(s)	1860
HpaII	CCGG	3 cut(s)	242, 1062, 1380
HphI	GGTGA	4 cut(s)	785, 922, 1178, 2333
Hpy166II	GTNNAC	6 cut(s)	527, 800, 1138, 1385, 1860, 1982
Hpy8I	GTNNAC	6 cut(s)	527, 800, 1138, 1385, 1860, 1982
Hpy99I	CGWCG	2 cut(s)	92, 2996
HpyAV	CCTTC	6 cut(s)	657, 951, 984, 1307, 2141, 2752
HpyCH4III	ACNGT	3 cut(s)	214, 269, 1517
HpyCH4IV	ACGT	5 cut(s)	1058, 1303, 1877, 2023, 2763
HpyF3I	CTNAG	9 cut(s)	132, 200, 1190, 1659, 1797, 1854, 2527, 2535, 2784
HpySE526I	ACGT	5 cut(s)	1058, 1303, 1877, 2023, 2763
Hsp92I	GRCGYC	1 cut(s)	2472
HspAI	GCGC	4 cut(s)	609, 908, 2472, 3078
KasI	GGCGCC	1 cut(s)	2471
KflI	GGGWCCC	1 cut(s)	882
Kpn2I	TCCGGA	2 cut(s)	241, 1061
Ksp22I	TGATCA	2 cut(s)	151, 1240
KspAI	GTTAAC	1 cut(s)	1860
LguI	GCTCTTC	2 cut(s)	1617, 3019
LmnI	GCTCC	9 cut(s)	77, 298, 1006, 1043, 1933, 1999, 2105, 2377, 3075
MaeI	CTAG	5 cut(s)	354, 837, 2003, 2232, 2820
MaeII	ACGT	5 cut(s)	1058, 1303, 1877, 2023, 2763
MaeIII	GTNAC	4 cut(s)	208, 1200, 1304, 1800
MbiI	CCGCTC	1 cut(s)	2665
MfeI	CAATTG	3 cut(s)	1776, 1920, 2169
MflI	RGATCY	1 cut(s)	3062
MhlI	GDGCHC	2 cut(s)	633, 2528
MlsI	TGGCCA	2 cut(s)	1084, 1808
MluNI	TGGCCA	2 cut(s)	1084, 1808
Mly113I	GGCGCC	1 cut(s)	2472
MmeI	TCCRAC	2 cut(s)	1175, 2099
Mox20I	TGGCCA	2 cut(s)	1084, 1808
Mph1103I	ATGCAT	3 cut(s)	147, 430, 1356
MroI	TCCGGA	2 cut(s)	241, 1061
MroXI	GAANNNNTTC	2 cut(s)	1722, 2593
MscI	TGGCCA	2 cut(s)	1084, 1808
MslI	CAYNNNNRTG	2 cut(s)	1587, 1709
Msp20I	TGGCCA	2 cut(s)	1084, 1808
MspA1I	CMGCKG	5 cut(s)	75, 275, 961, 2111, 2360
MspI	CCGG	3 cut(s)	242, 1062, 1380
MspR9I	CCNGG	7 cut(s)	101, 1428, 1508, 2091, 2365, 2464, 2975
MunI	CAATTG	3 cut(s)	1776, 1920, 2169
Mva1269I	GAATGC	3 cut(s)	507, 926, 2374
MvaI	CCWGG	7 cut(s)	101, 1428, 1508, 2091, 2365, 2464, 2975
MvnI	CGCG	3 cut(s)	1884, 2606, 2922
NarI	GGCGCC	1 cut(s)	2472
NcoI	CCATGG	1 cut(s)	2317
NmeAIII	GCCGAG	1 cut(s)	2159
NmuCI	GTSAC	1 cut(s)	208
NotI	GCGGCCGC	1 cut(s)	962
NruI	TCGCGA	2 cut(s)	1884, 2922
NsiI	ATGCAT	3 cut(s)	147, 430, 1356
NspI	RCATGY	3 cut(s)	149, 1261, 2291
NspV	TTCGAA	1 cut(s)	977
PaeI	GCATGC	2 cut(s)	149, 1261
PaeR7I	CTCGAG	1 cut(s)	2794
PaqCI	CACCTGC	2 cut(s)	1213, 1474
PciSI	GCTCTTC	2 cut(s)	1617, 3019
PctI	GAATGC	3 cut(s)	507, 926, 2374
PdmI	GAANNNNTTC	2 cut(s)	1722, 2593
PflFI	GACNNNGTC	1 cut(s)	2501
PflMI	CCANNNNNTGG	2 cut(s)	710, 1028
PinAI	ACCGGT	1 cut(s)	1379
PluTI	GGCGCC	1 cut(s)	2475
PpuMI	RGGWCCY	2 cut(s)	882, 971
PshAI	GACNNNNGTC	3 cut(s)	2571, 2874, 2910
PshBI	ATTAAT	1 cut(s)	303
PsiI	TTATAA	1 cut(s)	192
Psp1406I	AACGTT	2 cut(s)	1303, 2023
Psp5II	RGGWCCY	2 cut(s)	882, 971
Psp6I	CCWGG	7 cut(s)	99, 1426, 1506, 2089, 2363, 2462, 2973
PspEI	GGTNACC	1 cut(s)	1200
PspFI	CCCAGC	4 cut(s)	456, 626, 2654, 2702
PspGI	CCWGG	7 cut(s)	99, 1426, 1506, 2089, 2363, 2462, 2973
PspOMI	GGGCCC	1 cut(s)	629
PspPPI	RGGWCCY	2 cut(s)	882, 971
PstNI	CAGNNNCTG	3 cut(s)	278, 344, 2534
PsuI	RGATCY	1 cut(s)	3062
PsyI	GACNNNGTC	1 cut(s)	2501
PvuII	CAGCTG	2 cut(s)	275, 2360
RruI	TCGCGA	2 cut(s)	1884, 2922
RseI	CAYNNNNRTG	2 cut(s)	1587, 1709
SapI	GCTCTTC	2 cut(s)	1617, 3019
ScrFI	CCNGG	7 cut(s)	101, 1428, 1508, 2091, 2365, 2464, 2975
SduI	GDGCHC	2 cut(s)	633, 2528
SfoI	GGCGCC	1 cut(s)	2473
Sfr274I	CTCGAG	1 cut(s)	2794
SfuI	TTCGAA	1 cut(s)	977
SinI	GGWCC	5 cut(s)	8, 269, 882, 971, 1966
SlaI	CTCGAG	1 cut(s)	2794
SmiMI	CAYNNNNRTG	2 cut(s)	1587, 1709
SmlI	CTYRAG	5 cut(s)	483, 1267, 1678, 1994, 2794
SmoI	CTYRAG	5 cut(s)	483, 1267, 1678, 1994, 2794
SphI	GCATGC	2 cut(s)	149, 1261
SspDI	GGCGCC	1 cut(s)	2471
SspMI	CTAG	5 cut(s)	354, 837, 2003, 2232, 2820
StyD4I	CCNGG	7 cut(s)	99, 1426, 1506, 2089, 2363, 2462, 2973
StyI	CCWWGG	3 cut(s)	1079, 2317, 2520
TaaI	ACNGT	3 cut(s)	214, 269, 1517
TaiI	ACGT	5 cut(s)	1061, 1306, 1880, 2026, 2766
TaqI	TCGA	7 cut(s)	977, 2459, 2795, 2802, 2943, 2991, 3053
TauI	GCSGC	4 cut(s)	964, 967, 2111, 2929
TscAI	CASTG	6 cut(s)	345, 883, 1044, 1147, 1168, 1522
TseFI	GTSAC	1 cut(s)	208
Tsp45I	GTSAC	1 cut(s)	208
TspDTI	ATGAA	7 cut(s)	906, 1106, 2063, 2136, 2160, 2343, 2469
TspGWI	ACGGA	3 cut(s)	1192, 1958, 2491
TspRI	CASTG	6 cut(s)	345, 883, 1044, 1147, 1168, 1522
Tth111I	GACNNNGTC	1 cut(s)	2501
Van91I	CCANNNNNTGG	2 cut(s)	710, 1028
VpaK11BI	GGWCC	5 cut(s)	8, 269, 882, 971, 1966
VspI	ATTAAT	1 cut(s)	303
XagI	CCTNNNNNAGG	1 cut(s)	37
XapI	RAATTY	7 cut(s)	185, 285, 394, 1066, 1540, 2094, 2672
XceI	RCATGY	3 cut(s)	149, 1261, 2291
XcmI	CCANNNNNNNNNTGG	2 cut(s)	539, 1586
XhoI	CTCGAG	1 cut(s)	2794
XmiI	GTMKAC	2 cut(s)	1137, 1981
XmnI	GAANNNNTTC	2 cut(s)	1722, 2593
XspI	CTAG	5 cut(s)	354, 837, 2003, 2232, 2820
Zsp2I	ATGCAT	3 cut(s)	147, 430, 1356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.