Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7A

Orthogroup ID

OG0007032

Physical Location & Seq

Genomic Coordinates Forward (+)

32223948 .. 32232935

Gene Structure 8988 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7AG294500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2451 bp

ATGGCGGCCGGTGCGTATACGGATCAGCTGGAAGCTTACTTTCGGAGAGCGGATTTAGACGGAGATGGCAGGATCAGCGGAGCTGAGGCCGTCGCTTTCTTTCAGGGCGCCAATTTGCCCAAACCAGTTCTTGCTCAGATATGGATGCATGCGGATCAGAACAAAACTGGTTTCCTTGGTCGGCCAGAATTTTATAATGCTCTTAGACTTGTGACTGTGGCTCAAAGCAAGAGAGATTTAACGCCTGATATAGTCAAGGCCGCATTATACGGGCCAGCTGCTGCAAAAATTCCTGCTCCACAAATCAATCTTTCAGCCATATCTGCACCTCAAGCAAATCCTATGGCTGGTGCACCTGCTCCACAGATGGGTATGGGGACGCCACCAACATCTCAAAGTTTTGGGTTTAGAGGACCAGGAGCTCCTAATGCAGGTATGAACCAGAATTATTTTCAGCCACAGCAAAACCAGTCCATGAGACCTCCTCAAGGCATGACCCCTGGGATGCCTACTAGTGGATATTCCCGTCCACAACAGGGTGTGGGAGGTGGTATGGGGGGTCCCAATGTAATGAACTCAAATAATTGGCTTAGTGGAAGCGCTGGTGCACCTCCTTCTGGTCCAAGAGGGATTAGTCCTTCCATGCCCTCATCTACAACACAACCACAACCTTCAGTGTCAACATCTTCCCTGGCAACAGTGAATGACTCTAAATCATTAGTTCCTTCTGGAAATGGGTTTGCATCCAATTCGGGTTTCTCAGGTGATTTATTTTCTGCAGCCCCTGCTCAATCAAAGCCAGGAGCTCCTGGGTCAACATATTCTGCTAGTAGTGCACCTACGCCATCAGCCATTGTTCCAGTTTCAAGTGGGTCCCAATCTTCTAGTAAGCTCAATGCACTTGATTCACTGAGTGCATTCACAATGCAGCCTTCTGGTGGCCAATTTCAGCGGCCCCAGGCACCGTCAAACTCAAGCCCGCAAGTTTCAGCTGCGGGTCCTTCCTCTTTTGCATCACCTGCAATTTCAGTTGGAGTCGGAAACTCTACTTCTGAAAATTCACAGCCTCCTTGGCCAAAGATGAAGCCATCTGATGTTCAGAAATATACAAAAGTCTTTATGGAAGTAGACACTGACAGAGATGGGAAAATAACTGGAGAGCAGGCACGAAATCTATTTCTGAGCTGGAGGTTACCAAGAGAGGTTCTAAAGCAGGTGTGGGACTTATCTGATCAGGATAATGACAGCATGCTTTCCTTGAGAGAGTTCTGCTTTTCTCTCTATTTGATGGAACGTTACAGGGAAGGTCGGCCTCTTCCAGCTACACTTCCAAGCAATGTTATGTTGGATGAGACACTACTGTCAATGACAGGTCAACCAAAAGTGGGTTATGGAAATGCAGCTTGGAGTCCCCACCCTGGCTTTGGACAACATCAAGGGATGCAAGGTTCCCAGATGATGCCACCTGGCACTGGTTTGAGGCCACCAATTCAGGGAAACGCCCCTCAGGCTGATCGTACAGTGCAACCGAATCAGCAAAATTTGAGGGTGCCAGGGATGGAGGGCTTGGCTCAAAACCAGCTTGACAATGGGAAGCAGGATTCAGCCAACTCAAGACCTCAAGACCCTAACAGTGCACGGAAAGAGGTTGAAGAAACTGAGAATGTGATTTTGGACTCGAGAGAGAAGATTGAGTTCTATCGCACGAAAATGCAGGAACTTGTTCTGTATAAAAGCAGATGTGATAACAGATTAAATGAGATCACAGAAAGGGCACTGGCAGACAAGCGTGAGGCTGAACTACTGGGTAAGAAGTATGAAGAGAAATATAAACAAGTGGCAGAAATAGCATCTAAGTTAACCATTGAGGAGGCCACGTTTCGTGAAGTTCAGGAGAGGAAGATGGAATTGCATCAAGCAATTGTTAAAATGGAGCAAGGAGGTAGTGCTGATGGTATCCTTCAGGTCCGAGCTGATCGTATACAATATGATCTTGAGGAGCTAATAAAGGCTCTTACTGAACGTTGCAAGAAACATGGGCTAGACATGAAGTCAGCTGCAATAATTGAGCTTCCAACTGGTTGGCAACCTGGAATTCAAGACGGAGCAGCTGTTTGGGATGAAGATTGGGACAAGTTCGAAGATGAAGGATTTGGCAATGATCTCATAATTGATTCCTCAACAAAATCTGTCCAGAGAGAAAAGGCTTCCCCAGATCGTAGTTCAACTGCTGATTCATCATCGGTTACTGATGGAAAGTCAGGAATTTCTTACAGCAATGGTGATCATGCACATGAAAGTGAATCTGTTTTCACCCACGGTGAAGATGAACATGTAAGAAGTCCCAATGGCAGTCTAGCTGGAAGGACTGCTGTAGACAGCCCGTCTCGAGACTTCTCAGACATCCACTATGGTAAAAATTCCGAAGCAGATGGAGAAACACACGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000147 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005938 GO:0006810 GO:0006886 GO:0006897 GO:0006996 GO:0007010 GO:0008104 GO:0008150 GO:0009987 GO:0015031 GO:0015629 GO:0015833 GO:0016043 GO:0016192 GO:0022607 GO:0030029 GO:0030036 GO:0030479 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0033036 GO:0034613 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044396 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045184 GO:0046872 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061645 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0098657 GO:0099568

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

EF-hand_4 EF-hand_5

Protein Analysis

816

Amino Acids

87.68

Weight (kDa)

5.42

Isoelectric Point (pI)

49.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EH	PF12763	21 - 76	2e-07	EH domain
EH	PF12763	362 - 444	3e-12	EH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007032)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20760
fragaria_vesca	FvH4_5g25580 FvH4_5g25580
malus_domestica	MD06G1007400.v1.1
prunus_persica	Prupe.5G009300_v2.0.a1 Prupe.5G009300_v2.0.a1
pyrus_communis	pycom06g00650 pycom06g00660
rosa_chinensis	RchiOBHm_Chr7g0215891 RchiOBHm_Chr7g0219801 RchiOBHm_Chr7g0219941
rosa_laevigata	RLG00000002612
rosa_multiflora	Rmu_co8383945.1_g000001 Rmu_sc0006240.1_g000011
rosa_roxburghii	Rroxscaffold_3G00243830
rosa_rugosa	Rorug07G0156400 Rorug07G0156500
rosa_samantha	Rh7AG294500 Rh7AG298200 Rh7BG286300 Rh7CG314500 Rh7CG318200 Rh7CG318300 Rh7DG297400
rosa_wichuraiana	Rw7G025020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	195
AarI	CACCTGC	3 cut(s)	364, 1027, 1204
AasI	GACNNNNNNGTC	1 cut(s)	1360
Acc36I	ACCTGC	4 cut(s)	364, 422, 1027, 1204
AccB1I	GGYRCC	3 cut(s)	107, 961, 1549
AccBSI	CCGCTC	1 cut(s)	50
AccI	GTMKAC	4 cut(s)	17, 1128, 1981, 2376
AciI	CCGC	8 cut(s)	5, 50, 78, 152, 261, 952, 980, 995
AclI	AACGTT	2 cut(s)	1294, 2023
AclWI	GGATC	3 cut(s)	30, 80, 162
AcoI	YGGCCR	4 cut(s)	6, 182, 940, 1073
AcsI	RAATTY	7 cut(s)	188, 288, 1057, 1540, 2094, 2265, 2419
AcuI	CTGAAG	2 cut(s)	657, 1946
AcyI	GRCGYC	2 cut(s)	108, 380
AdeI	CACNNNGTG	1 cut(s)	914
AfaI	GTAC	1 cut(s)	1519
AfeI	AGCGCT	1 cut(s)	601
AflIII	ACRYGT	1 cut(s)	2332
AgsI	TTSAA	4 cut(s)	867, 1652, 2099, 2226
AhdI	GACNNNNNGTC	2 cut(s)	2050, 2383
AhlI	ACTAGT	1 cut(s)	512
AjuI	GAANNNNNNNTTGG	6 cut(s)	740, 772, 1387, 1419, 1655, 1687
AloI	GAACNNNNNNTCC	2 cut(s)	1432, 1464
Alw21I	GWGCWC	6 cut(s)	355, 424, 610, 808, 838, 1639
Alw26I	GTCTC	4 cut(s)	472, 1346, 2385, 2391
Alw44I	GTGCAC	4 cut(s)	351, 606, 834, 1635
AlwI	GGATC	3 cut(s)	30, 80, 162
AlwNI	CAGNNNCTG	2 cut(s)	281, 785
Ama87I	CYCGRG	2 cut(s)	1678, 2388
Aor51HI	AGCGCT	1 cut(s)	601
ApaLI	GTGCAC	4 cut(s)	351, 606, 834, 1635
ApeKI	GCWGC	8 cut(s)	278, 281, 779, 928, 992, 1400, 2057, 2108
ApoI	RAATTY	7 cut(s)	188, 288, 1057, 1540, 2094, 2265, 2419
Asp700I	GAANNNNTTC	1 cut(s)	1722
AspLEI	GCGC	2 cut(s)	110, 602
AspS9I	GGNCC	8 cut(s)	272, 413, 560, 620, 873, 954, 998, 1966
AsuHPI	GGTGA	5 cut(s)	776, 1008, 2294, 2305, 2333
AsuII	TTCGAA	1 cut(s)	2139
AvaI	CYCGRG	2 cut(s)	1678, 2388
AvaII	GGWCC	6 cut(s)	413, 560, 620, 873, 998, 1966
AxyI	CCTNAGG	1 cut(s)	1506
BaeGI	GKGCMC	5 cut(s)	355, 610, 838, 1639, 1777
BalI	TGGCCA	2 cut(s)	942, 1075
BanI	GGYRCC	3 cut(s)	107, 961, 1549
BanII	GRGCYC	2 cut(s)	424, 808
Bbv12I	GWGCWC	6 cut(s)	355, 424, 610, 808, 838, 1639
BbvCI	CCTCAGC	1 cut(s)	84
BbvI	GCAGC	8 cut(s)	265, 268, 791, 940, 979, 1412, 2044, 2120
BceAI	ACGGC	1 cut(s)	74
BcgI	CGANNNNNNTGC	2 cut(s)	732, 766
BciVI	GTATCC	1 cut(s)	1967
BclI	TGATCA	2 cut(s)	1231, 2284
BcoDI	GTCTC	4 cut(s)	472, 1346, 2385, 2391
BcuI	ACTAGT	1 cut(s)	512
BfaI	CTAG	5 cut(s)	513, 828, 885, 2042, 2357
BfmI	CTRYAG	2 cut(s)	777, 2373
BfoI	RGCGCY	2 cut(s)	111, 603
BfuAI	ACCTGC	4 cut(s)	364, 422, 1027, 1204
BfuI	GTATCC	1 cut(s)	1967
BglI	GCCNNNNNGGC	2 cut(s)	1072, 1508
Bme18I	GGWCC	6 cut(s)	413, 560, 620, 873, 998, 1966
BmeRI	GACNNNNNGTC	2 cut(s)	2050, 2383
BmeT110I	CYCGRG	2 cut(s)	1678, 2388
BmgT120I	GGNCC	8 cut(s)	272, 413, 560, 620, 873, 954, 998, 1966
BmrI	ACTGGG	1 cut(s)	1814
BmsI	GCATC	8 cut(s)	135, 495, 752, 1022, 1431, 1449, 1859, 1921
BmuI	ACTGGG	1 cut(s)	1814
BplI	GAGNNNNNCTC	2 cut(s)	469, 501
BpmI	CTGGAG	2 cut(s)	1176, 1207
Bpu10I	CCTNAGC	1 cut(s)	84
Bpu14I	TTCGAA	1 cut(s)	2139
BpuEI	CTTGAG	7 cut(s)	315, 471, 958, 1279, 1597, 1605, 2015
BsaHI	GRCGYC	2 cut(s)	108, 380
BsaI	GGTCTC	1 cut(s)	472
BsaXI	ACNNNNNCTCC	4 cut(s)	1862, 1892, 2097, 2127
Bse118I	RCCGGY	1 cut(s)	8
Bse1I	ACTGG	9 cut(s)	125, 172, 469, 860, 1159, 1477, 1782, 1809, 2083
Bse21I	CCTNAGG	1 cut(s)	1506
Bse3DI	GCAATG	3 cut(s)	1342, 2164, 2284
BseGI	GGATG	8 cut(s)	150, 510, 743, 1354, 1446, 1563, 2125, 2403
BseMI	GCAATG	3 cut(s)	1342, 2164, 2284
BseMII	CTCAG	8 cut(s)	75, 149, 774, 902, 1172, 1520, 1650, 2412
BseNI	ACTGG	9 cut(s)	125, 172, 469, 860, 1159, 1477, 1782, 1809, 2083
BseRI	GAGGAG	3 cut(s)	474, 1883, 2012
BseSI	GKGCMC	5 cut(s)	355, 610, 838, 1639, 1777
BseX3I	CGGCCG	1 cut(s)	6
BseXI	GCAGC	8 cut(s)	265, 268, 791, 940, 979, 1412, 2044, 2120
BsgI	GTGCAG	1 cut(s)	309
Bsh1285I	CGRYCG	1 cut(s)	9
BshNI	GGYRCC	3 cut(s)	107, 961, 1549
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	6 cut(s)	355, 424, 610, 808, 838, 1639
BsiHKCI	CYCGRG	2 cut(s)	1678, 2388
BsiSI	CCGG	1 cut(s)	9
BslFI	GGGAC	7 cut(s)	391, 546, 859, 1235, 1395, 2144, 2328
BsmAI	GTCTC	4 cut(s)	472, 1346, 2385, 2391
BsmBI	CGTCTC	1 cut(s)	2391
BsmFI	GGGAC	7 cut(s)	391, 546, 859, 1235, 1395, 2144, 2328
BsmI	GAATGC	1 cut(s)	917
Bso31I	GGTCTC	1 cut(s)	472
BsoBI	CYCGRG	2 cut(s)	1678, 2388
Bsp119I	TTCGAA	1 cut(s)	2139
Bsp1286I	GDGCHC	7 cut(s)	355, 424, 610, 808, 838, 1639, 1777
BspACI	CCGC	8 cut(s)	5, 50, 78, 152, 261, 952, 980, 995
BspCNI	CTCAG	8 cut(s)	76, 148, 773, 903, 1173, 1519, 1651, 2411
BspMAI	CTGCAG	1 cut(s)	781
BspMI	ACCTGC	4 cut(s)	364, 422, 1027, 1204
BspPI	GGATC	3 cut(s)	30, 80, 162
BspT104I	TTCGAA	1 cut(s)	2139
BspT107I	GGYRCC	3 cut(s)	107, 961, 1549
BspTNI	GGTCTC	1 cut(s)	472
BsrBI	CCGCTC	1 cut(s)	50
BsrDI	GCAATG	3 cut(s)	1342, 2164, 2284
BsrFI	RCCGGY	1 cut(s)	8
BsrI	ACTGG	9 cut(s)	125, 172, 469, 860, 1159, 1477, 1782, 1809, 2083
BssAI	RCCGGY	1 cut(s)	8
BssNAI	GTATAC	2 cut(s)	18, 1982
BssNI	GRCGYC	2 cut(s)	108, 380
BssT1I	CCWWGG	2 cut(s)	175, 1070
Bst1107I	GTATAC	2 cut(s)	18, 1982
Bst4CI	ACNGT	7 cut(s)	217, 700, 966, 1362, 1522, 1634, 2321
Bst6I	CTCTTC	2 cut(s)	1320, 1815
BstACI	GRCGYC	2 cut(s)	108, 380
BstAPI	GCANNNNNTGC	2 cut(s)	785, 1019
BstBI	TTCGAA	1 cut(s)	2139
BstC8I	GCNNGC	5 cut(s)	150, 276, 980, 1164, 1250
BstDSI	CCRYGG	1 cut(s)	2317
BstEII	GGTNACC	1 cut(s)	1191
BstENI	CCTNNNNNAGG	2 cut(s)	429, 486
BstF5I	GGATG	8 cut(s)	150, 510, 743, 1354, 1446, 1563, 2125, 2403
BstH2I	RGCGCY	2 cut(s)	111, 603
BstHHI	GCGC	2 cut(s)	110, 602
BstMAI	GTCTC	4 cut(s)	472, 1346, 2385, 2391
BstMCI	CGRYCG	1 cut(s)	9
BstNSI	RCATGY	3 cut(s)	152, 1252, 2336
BstPI	GGTNACC	1 cut(s)	1191
BstSFI	CTRYAG	2 cut(s)	777, 2373
BstSLI	GKGCMC	5 cut(s)	355, 610, 838, 1639, 1777
BstV1I	GCAGC	8 cut(s)	265, 268, 791, 940, 979, 1412, 2044, 2120
BstXI	CCANNNNNNTGG	1 cut(s)	2082
BstZ17I	GTATAC	2 cut(s)	18, 1982
BstZI	CGGCCG	1 cut(s)	6
Bsu36I	CCTNAGG	1 cut(s)	1506
BsuI	GTATCC	1 cut(s)	1967
BtgI	CCRYGG	1 cut(s)	2317
BtsCI	GGATG	8 cut(s)	150, 510, 743, 1354, 1446, 1563, 2125, 2403
BtsIMutI	CAGTG	8 cut(s)	681, 705, 908, 1131, 1470, 1527, 1639, 1775
BveI	ACCTGC	4 cut(s)	364, 422, 1027, 1204
Cac8I	GCNNGC	5 cut(s)	150, 276, 980, 1164, 1250
CaiI	CAGNNNCTG	2 cut(s)	281, 785
CfoI	GCGC	2 cut(s)	110, 602
Cfr10I	RCCGGY	1 cut(s)	8
Cfr13I	GGNCC	8 cut(s)	272, 413, 560, 620, 873, 954, 998, 1966
CseI	GACGC	1 cut(s)	388
Csp6I	GTAC	1 cut(s)	1518
CviQI	GTAC	1 cut(s)	1518
DinI	GGCGCC	1 cut(s)	109
DraIII	CACNNNGTG	1 cut(s)	914
DrdI	GACNNNNNNGTC	1 cut(s)	1360
DriI	GACNNNNNGTC	2 cut(s)	2050, 2383
DseDI	GACNNNNNNGTC	1 cut(s)	1360
EaeI	YGGCCR	4 cut(s)	6, 182, 940, 1073
EagI	CGGCCG	1 cut(s)	6
Eam1104I	CTCTTC	2 cut(s)	1320, 1815
Eam1105I	GACNNNNNGTC	2 cut(s)	2050, 2383
EarI	CTCTTC	2 cut(s)	1320, 1815
Ecl136II	GAGCTC	2 cut(s)	422, 806
EclXI	CGGCCG	1 cut(s)	6
Eco130I	CCWWGG	2 cut(s)	175, 1070
Eco24I	GRGCYC	2 cut(s)	424, 808
Eco31I	GGTCTC	1 cut(s)	472
Eco47I	GGWCC	6 cut(s)	413, 560, 620, 873, 998, 1966
Eco47III	AGCGCT	1 cut(s)	601
Eco52I	CGGCCG	1 cut(s)	6
Eco53kI	GAGCTC	2 cut(s)	422, 806
Eco57I	CTGAAG	2 cut(s)	657, 1946
Eco81I	CCTNAGG	1 cut(s)	1506
Eco88I	CYCGRG	2 cut(s)	1678, 2388
Eco91I	GGTNACC	1 cut(s)	1191
EcoICRI	GAGCTC	2 cut(s)	422, 806
EcoNI	CCTNNNNNAGG	2 cut(s)	429, 486
EcoO109I	RGGNCCY	3 cut(s)	560, 873, 998
EcoO65I	GGTNACC	1 cut(s)	1191
EcoRI	GAATTC	1 cut(s)	2094
EcoT14I	CCWWGG	2 cut(s)	175, 1070
EcoT22I	ATGCAT	1 cut(s)	150
EcoT38I	GRGCYC	2 cut(s)	424, 808
EgeI	GGCGCC	1 cut(s)	109
EheI	GGCGCC	1 cut(s)	109
ErhI	CCWWGG	2 cut(s)	175, 1070
Esp3I	CGTCTC	1 cut(s)	2391
FaqI	GGGAC	7 cut(s)	391, 546, 859, 1235, 1395, 2144, 2328
FauI	CCCGC	2 cut(s)	987, 988
FbaI	TGATCA	2 cut(s)	1231, 2284
FblI	GTMKAC	4 cut(s)	17, 1128, 1981, 2376
FokI	GGATG	8 cut(s)	157, 517, 730, 1361, 1453, 1570, 2132, 2390
FriOI	GRGCYC	2 cut(s)	424, 808
FspBI	CTAG	5 cut(s)	513, 828, 885, 2042, 2357
GlaI	GCGC	2 cut(s)	109, 601
GsuI	CTGGAG	2 cut(s)	1176, 1207
HaeII	RGCGCY	2 cut(s)	111, 603
HapII	CCGG	1 cut(s)	9
HgaI	GACGC	1 cut(s)	388
HhaI	GCGC	2 cut(s)	110, 602
Hin1I	GRCGYC	2 cut(s)	108, 380
Hin6I	GCGC	2 cut(s)	108, 600
HinP1I	GCGC	2 cut(s)	108, 600
HincII	GTYRAC	4 cut(s)	681, 816, 1376, 1860
HindII	GTYRAC	4 cut(s)	681, 816, 1376, 1860
HindIII	AAGCTT	1 cut(s)	33
HpaI	GTTAAC	1 cut(s)	1860
HpaII	CCGG	1 cut(s)	9
HphI	GGTGA	5 cut(s)	776, 1008, 2294, 2305, 2333
Hpy99I	CGWCG	1 cut(s)	95
HpyCH4III	ACNGT	7 cut(s)	217, 700, 966, 1362, 1522, 1634, 2321
HpyCH4IV	ACGT	3 cut(s)	1294, 1877, 2023
HpySE526I	ACGT	3 cut(s)	1294, 1877, 2023
Hsp92I	GRCGYC	2 cut(s)	108, 380
HspAI	GCGC	2 cut(s)	108, 600
KasI	GGCGCC	1 cut(s)	107
KflI	GGGWCCC	2 cut(s)	560, 873
Ksp22I	TGATCA	2 cut(s)	1231, 2284
KspAI	GTTAAC	1 cut(s)	1860
Lsp1109I	GCAGC	8 cut(s)	265, 268, 791, 940, 979, 1412, 2044, 2120
LweI	GCATC	8 cut(s)	135, 495, 752, 1022, 1431, 1449, 1859, 1921
MaeI	CTAG	5 cut(s)	513, 828, 885, 2042, 2357
MaeII	ACGT	3 cut(s)	1294, 1877, 2023
MaeIII	GTNAC	4 cut(s)	211, 1191, 1295, 2245
MbiI	CCGCTC	1 cut(s)	50
MfeI	CAATTG	1 cut(s)	1920
MhlI	GDGCHC	7 cut(s)	355, 424, 610, 808, 838, 1639, 1777
MlsI	TGGCCA	2 cut(s)	942, 1075
MluNI	TGGCCA	2 cut(s)	942, 1075
Mly113I	GGCGCC	1 cut(s)	108
MlyI	GAGTC	4 cut(s)	701, 1044, 1417, 1670
MmeI	TCCRAC	4 cut(s)	1012, 1018, 1326, 2099
Mox20I	TGGCCA	2 cut(s)	942, 1075
Mph1103I	ATGCAT	1 cut(s)	150
MroXI	GAANNNNTTC	1 cut(s)	1722
MscI	TGGCCA	2 cut(s)	942, 1075
MseI	TTAA	4 cut(s)	239, 1754, 1859, 1926
MslI	CAYNNNNRTG	3 cut(s)	1709, 2292, 2298
Msp20I	TGGCCA	2 cut(s)	942, 1075
MspA1I	CMGCKG	7 cut(s)	28, 78, 278, 952, 992, 2057, 2111
MspI	CCGG	1 cut(s)	9
MunI	CAATTG	1 cut(s)	1920
Mva1269I	GAATGC	1 cut(s)	917
NarI	GGCGCC	1 cut(s)	108
NmuCI	GTSAC	1 cut(s)	211
NsiI	ATGCAT	1 cut(s)	150
NspI	RCATGY	3 cut(s)	152, 1252, 2336
NspV	TTCGAA	1 cut(s)	2139
PaeI	GCATGC	2 cut(s)	152, 1252
PaeR7I	CTCGAG	2 cut(s)	1678, 2388
PaqCI	CACCTGC	3 cut(s)	364, 1027, 1204
PasI	CCCWGGG	1 cut(s)	500
PciI	ACATGT	1 cut(s)	2332
PctI	GAATGC	1 cut(s)	917
PdmI	GAANNNNTTC	1 cut(s)	1722
PfeI	GAWTC	6 cut(s)	905, 1531, 1601, 2174, 2234, 2303
PleI	GAGTC	4 cut(s)	701, 1043, 1416, 1670
PluTI	GGCGCC	1 cut(s)	111
PpsI	GAGTC	4 cut(s)	701, 1043, 1416, 1670
PpuMI	RGGWCCY	3 cut(s)	560, 873, 998
PscI	ACATGT	1 cut(s)	2332
PsiI	TTATAA	1 cut(s)	195
Psp124BI	GAGCTC	2 cut(s)	424, 808
Psp1406I	AACGTT	2 cut(s)	1294, 2023
Psp5II	RGGWCCY	3 cut(s)	560, 873, 998
PspEI	GGTNACC	1 cut(s)	1191
PspPI	GGNCC	8 cut(s)	272, 413, 560, 620, 873, 954, 998, 1966
PspPPI	RGGWCCY	3 cut(s)	560, 873, 998
PstI	CTGCAG	1 cut(s)	781
PstNI	CAGNNNCTG	2 cut(s)	281, 785
PvuII	CAGCTG	5 cut(s)	28, 278, 992, 2057, 2111
RsaI	GTAC	1 cut(s)	1519
RsaNI	GTAC	1 cut(s)	1518
RseI	CAYNNNNRTG	3 cut(s)	1709, 2292, 2298
SacI	GAGCTC	2 cut(s)	424, 808
SaqAI	TTAA	4 cut(s)	239, 1754, 1859, 1926
Sau96I	GGNCC	8 cut(s)	272, 413, 560, 620, 873, 954, 998, 1966
SchI	GAGTC	4 cut(s)	701, 1044, 1417, 1670
SduI	GDGCHC	7 cut(s)	355, 424, 610, 808, 838, 1639, 1777
SfaNI	GCATC	8 cut(s)	135, 495, 752, 1022, 1431, 1449, 1859, 1921
SfcI	CTRYAG	2 cut(s)	777, 2373
SfoI	GGCGCC	1 cut(s)	109
Sfr274I	CTCGAG	2 cut(s)	1678, 2388
SfuI	TTCGAA	1 cut(s)	2139
SinI	GGWCC	6 cut(s)	413, 560, 620, 873, 998, 1966
SlaI	CTCGAG	2 cut(s)	1678, 2388
SmiMI	CAYNNNNRTG	3 cut(s)	1709, 2292, 2298
SmlI	CTYRAG	9 cut(s)	330, 486, 973, 1258, 1612, 1620, 1678, 1994, 2388
SmoI	CTYRAG	9 cut(s)	330, 486, 973, 1258, 1612, 1620, 1678, 1994, 2388
SpeI	ACTAGT	1 cut(s)	512
SphI	GCATGC	2 cut(s)	152, 1252
SsiI	CCGC	8 cut(s)	5, 50, 78, 152, 261, 952, 980, 995
SspDI	GGCGCC	1 cut(s)	107
SspMI	CTAG	5 cut(s)	513, 828, 885, 2042, 2357
SstI	GAGCTC	2 cut(s)	424, 808
StyI	CCWWGG	2 cut(s)	175, 1070
TaaI	ACNGT	7 cut(s)	217, 700, 966, 1362, 1522, 1634, 2321
TaiI	ACGT	3 cut(s)	1297, 1880, 2026
TaqI	TCGA	3 cut(s)	1679, 2139, 2389
TauI	GCSGC	3 cut(s)	8, 263, 955
TfiI	GAWTC	6 cut(s)	905, 1531, 1601, 2174, 2234, 2303
Tru1I	TTAA	4 cut(s)	239, 1754, 1859, 1926
Tru9I	TTAA	4 cut(s)	239, 1754, 1859, 1926
TscAI	CASTG	8 cut(s)	681, 705, 915, 1138, 1477, 1527, 1639, 1782
TseFI	GTSAC	1 cut(s)	211
TseI	GCWGC	8 cut(s)	278, 281, 779, 928, 992, 1400, 2057, 2108
Tsp45I	GTSAC	1 cut(s)	211
TspGWI	ACGGA	4 cut(s)	35, 75, 1654, 2118
TspRI	CASTG	8 cut(s)	681, 705, 915, 1138, 1477, 1527, 1639, 1782
VneI	GTGCAC	4 cut(s)	351, 606, 834, 1635
VpaK11BI	GGWCC	6 cut(s)	413, 560, 620, 873, 998, 1966
XagI	CCTNNNNNAGG	2 cut(s)	429, 486
XapI	RAATTY	7 cut(s)	188, 288, 1057, 1540, 2094, 2265, 2419
XceI	RCATGY	3 cut(s)	152, 1252, 2336
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1421, 1586
XhoI	CTCGAG	2 cut(s)	1678, 2388
XmiI	GTMKAC	4 cut(s)	17, 1128, 1981, 2376
XmnI	GAANNNNTTC	1 cut(s)	1722
XspI	CTAG	5 cut(s)	513, 828, 885, 2042, 2357
Zsp2I	ATGCAT	1 cut(s)	150

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.