Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0007032

Physical Location & Seq

Genomic Coordinates Forward (+)

17108836 .. 17117098

Gene Structure 8263 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g25580.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3054 bp

ATGGCGGCCGGCGCGTACACGGATCAGCTCGAAGCTTACTTCAGGCGAGCGGATTTGGACGGAGACGGCAGGATCAGCGGTGCTGAGGCCGTCGCTTTCTTTCAGGGCGCCAATTTGCCCAAGCCAGTGCTTGCTCAGATATGGATGCATGCGGATCAGAACAAAACTGGTTTCCTTGGTCGGCCAGAATTTTATAATGCTCTTAGACTTGTGACTGTGGCTCAAAGCAAGAGAGATTTAACGCCGGATATTGTCAAGGCGGCATTATACGGGCCAGCTGCTGCAAAGATTCCTCCCCCACAAATTAATCTTTCAGCCCTAGCTGCACCTCAAGCAAATCCCATGGCTGGTGCACCTGCTCCACAGATGGGTATAGGGACACCATCAACATCTCAAAGTTTCGGGTTTAGAGGATCTGGAGCTCCCAATGCAGGTATGAACCAGAATTATTTCCAGCCACAGCAAAACCAGTCCATGAGACCTCCTCAAGGCATGCCCCCTGGGATGCCTAATACTATCCATTCCCGACCACAACAGGGATTTGGAGGTGGTGTGGGGGGTCCCAATGTAATGAACTCAAATAATTGGCTTAGTGGAAGCACTGGTGCACCTCCTCCTGGTCCCAGAGGAATTAGTTCCTCCATGCCCTCATCTACAACACAACCACAACCTCCGGTGTCATCGTCTTCCCTGCCTACTGTTAATGACAGTAGATCATTAGTTCCTTCTGGAAATGGGTTTGCTTCGAATTCGGGTTTCTCAGGTGGTGTATTTTCGGCAACCCCTCAATCAAAGCCAGGAGCTTCTGGGTCAACATATTCTGCTAGTAGTGCACCTATGCCATCAGCCATTGTTCCAGTTTCAAGTGGGTCTCAATCTTCTAGTAAGCTCAGTGCACTTGATTCACTGAGTGCATTCACAATGCAACCTTCTGGTGGCCAGTTTCAGCAGCCCCATGCACCATCAAACCCAAGCCAGCAAGTTTCAGCTGCGGTTACTACCTCTTTTTCATCACCTTCAATTTCAGTTGGAGTTGGAAACTCTAATTCTGAGAATTCACAGCCTCCTTGGCCAAAGATGAAGCCATCTGATGTTCAGAAATACACAAAAGTCTTCATGGAAGTAGACAGTGACAGAGATGGTAAAGTCACTGGAGAGCAGGCACGAAATTTATTTCTCAGTTGGAGGTTGCCAAGAGAGGTTCTAAAGCAGGTGTGGGACTTATCTGATCAGGACAATGACAGCATGCTTTCCTTAAGAGAGTTCTGCTTTTCTCTCTATTTGATGGAGCGTTACCGGGAAGGTCGGCCTCTTCCAGCTACACTTCCAAGCGATGTTATGTTGGATGAGACACTGATATCGATGACAGGTCAACCAAAAGTGGGTTATGGAAATGCAGCTTGGAGTCCCCACCCAGGCTTTGGACAACATCAAGGAATGCAAGGTTCCCAGATGATGCCACCTGGTACTGGTTTGAAGCCACCAATTCAGGGAAATGCCCCTCAGGGTGATCGTGCAATGCAACCGAATCAGCAAAATTTGAGAGTGCGAGGAATGGTGGCTCCAAACCAGCTTGACAATGGGAAGCAGGATTCAGCGAACTCAAAACCTCAAGACCCTAGCGAGGCAGAGAAAAAGGTTGAAGAAATTGAGAATGTGATTCTGGACTCAAGAGAGAAGATTGAGTTCTATCGCACAAAAATGCAGGAACTTGTGCTGTATAAGAGCAGATGTGATAACAGATTAAATGAGATCACGGAAAGGGCACTGGCTGATAAGCGTGAGGCTGAACTACTGGCTAAGAAATATGAAGAGAAATATAAACAAGTGGCAGAAATAGCATCTAAGTTGACCATTGAAGAGGCCATGTTTCGCGAAGTTCAGGAGAGGAAGACTGAATTGCATCAAGCAATTGTTAAAATGGAGCAAGGAGGTAGTGCTGATGGTATCCTTCAGGTCCGTGCTGATCGTATACAATATGATCTTGAGGAACTAATAAAGGCTCTTACTGAACGTTGCAAGAAACATGGGATAGAGATGAAGTCAGCTGCAATAATCGAGCTTCCAACTGGCTGGCAACCTGGAATTCAAGACGGAGCAGCTGTTTGGGATGAAGAATGGGACAAGTTCGAAGACGAAGGATTTGGCAATGATCTCAAAATTGATTCCTCAACAAAACCTGACTCTGGATCTGTCCAGAGAGAAAAGGCTTCCCCAGATCGTAGTTCAACTCCTGATTCATCATTTGTTGCTAATGGAAAGTCAGGAATTTCTTCCAGCAATGGTGATCATGCACATGAAAGTGATTCTGTTTTCACCCACAGTGAAGATGAACATGTAAGAAGTCCCAATGGCAGTCTAGCTGGAAGGACTGCTGTAGACAGCCCGTCTCGAGACTTCTCAGATATCCACTATGGTAAAAATTCCGAAGCAGATGGAGAAACACATGGAAGTTTTGATGAATCAACCTGGGGTGCCTTTGACAATAATGATGATATTGACTCTGTTTGGGGTTTCAATGCTGACAAGGGCAAGGACTCAGATTCTGAGAAGCACCGAGATTTCTTTGGATCTGATGACTTCGGTGTCAACCCAGTAAGAACTGGATTCCCGAATGCAGATACCGCCTTTCAGAAAAAGAGCATTTTCTTTGAGGAGTCTGTTCCTAGCACACCAGCCTCGAGGTTTGCCAACTCGCCAAGATACAGTGAGGCTGGGGATCAGTACTTTGACAGTGGTTTCTCAAGGTTCGATTCATTCAGTAGCAGGCAAGACAGTGGTTTTTCTTCTCAACCTGAGAAGTTCTCAAGGTTCGATTCCATAAATAGCACTAGAGATTTTGGCCACTCAAGGTTTGATTCCATAAGCAGCAGCAGAGACTTTGGCCAAAGTCATGGCCTCACAAGGTTTGACTCCATAAACAGCACAAAAGATTTTGGCCAGGGTACATACTCTTTCGATGACTCAGATCCATTTGGCTCTTCTGGCCCCTTCAAGGTCTCATCAGAGAGTCAAAATGCAAAGAAGGGTTCTGATAATTGGAATGCTTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000147 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005938 GO:0006810 GO:0006886 GO:0006897 GO:0006996 GO:0007010 GO:0008104 GO:0008150 GO:0009987 GO:0015031 GO:0015629 GO:0015833 GO:0016043 GO:0016192 GO:0022607 GO:0030029 GO:0030036 GO:0030479 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0033036 GO:0034613 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044396 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045184 GO:0046872 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061645 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0098657 GO:0099568

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

EF-hand_4 EF-hand_5

Protein Analysis

1018

Amino Acids

110.2

Weight (kDa)

5.22

Isoelectric Point (pI)

51.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EH	PF12763	21 - 76	2.8e-07	EH domain
EH	PF12763	361 - 445	5.6e-12	EH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007032)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20760
fragaria_vesca	FvH4_5g25580 FvH4_5g25580
malus_domestica	MD06G1007400.v1.1
prunus_persica	Prupe.5G009300_v2.0.a1 Prupe.5G009300_v2.0.a1
pyrus_communis	pycom06g00650 pycom06g00660
rosa_chinensis	RchiOBHm_Chr7g0215891 RchiOBHm_Chr7g0219801 RchiOBHm_Chr7g0219941
rosa_laevigata	RLG00000002612
rosa_multiflora	Rmu_co8383945.1_g000001 Rmu_sc0006240.1_g000011
rosa_roxburghii	Rroxscaffold_3G00243830
rosa_rugosa	Rorug07G0156400 Rorug07G0156500
rosa_samantha	Rh7AG294500 Rh7AG298200 Rh7BG286300 Rh7CG314500 Rh7CG318200 Rh7CG318300 Rh7DG297400
rosa_wichuraiana	Rw7G025020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	195
AarI	CACCTGC	2 cut(s)	364, 1201
AasI	GACNNNNNNGTC	1 cut(s)	2585
AbsI	CCTCGAGG	1 cut(s)	2680
Acc36I	ACCTGC	3 cut(s)	364, 422, 1201
AccB1I	GGYRCC	2 cut(s)	107, 2474
AccB7I	CCANNNNNTGG	1 cut(s)	1421
AccBSI	CCGCTC	1 cut(s)	50
AccI	GTMKAC	3 cut(s)	1125, 1972, 2379
AccII	CGCG	2 cut(s)	14, 1875
AciI	CCGC	7 cut(s)	5, 50, 78, 152, 260, 992, 2625
AclI	AACGTT	1 cut(s)	2014
AclWI	GGATC	8 cut(s)	30, 80, 162, 421, 2197, 2578, 2727, 2963
AcoI	YGGCCR	7 cut(s)	6, 182, 937, 1070, 2842, 2884, 2938
AcsI	RAATTY	8 cut(s)	188, 748, 1054, 1168, 1537, 2085, 2268, 2422
AcuI	CTGAAG	2 cut(s)	25, 1937
AcyI	GRCGYC	1 cut(s)	108
AdeI	CACNNNGTG	1 cut(s)	911
AfaI	GTAC	4 cut(s)	17, 1468, 2726, 2947
AflII	CTTAAG	1 cut(s)	1255
AflIII	ACRYGT	1 cut(s)	2335
AgsI	TTSAA	9 cut(s)	864, 1020, 1477, 1643, 1859, 2090, 2229, 2518, 2995
AhdI	GACNNNNNGTC	1 cut(s)	2386
AjnI	CCWGG	8 cut(s)	499, 616, 796, 1414, 1462, 2080, 2468, 2940
AjuI	GAANNNNNNNTTGG	2 cut(s)	1384, 1416
Alw21I	GWGCWC	5 cut(s)	355, 424, 610, 835, 898
Alw26I	GTCTC	8 cut(s)	57, 472, 876, 1343, 2388, 2394, 2871, 3004
Alw44I	GTGCAC	4 cut(s)	351, 606, 831, 894
AlwI	GGATC	8 cut(s)	30, 80, 162, 421, 2197, 2578, 2727, 2963
AlwNI	CAGNNNCTG	2 cut(s)	281, 2546
Ama87I	CYCGRG	2 cut(s)	2391, 2680
ApaLI	GTGCAC	4 cut(s)	351, 606, 831, 894
ApoI	RAATTY	8 cut(s)	188, 748, 1054, 1168, 1537, 2085, 2268, 2422
ArsI	GACNNNNNNTTYG	2 cut(s)	2126, 2158
AseI	ATTAAT	1 cut(s)	306
Asp700I	GAANNNNTTC	4 cut(s)	449, 634, 2272, 3047
AspLEI	GCGC	2 cut(s)	14, 110
AspS9I	GGNCC	5 cut(s)	272, 560, 620, 1957, 2987
AsuC2I	CCSGG	1 cut(s)	1298
AsuHPI	GGTGA	4 cut(s)	1005, 1520, 2297, 2308
AsuII	TTCGAA	2 cut(s)	746, 2130
AvaI	CYCGRG	2 cut(s)	2391, 2680
AvaII	GGWCC	3 cut(s)	560, 620, 1957
AxyI	CCTNAGG	1 cut(s)	1503
BaeGI	GKGCMC	5 cut(s)	355, 610, 835, 898, 1768
BalI	TGGCCA	5 cut(s)	939, 1072, 2844, 2886, 2940
BanI	GGYRCC	2 cut(s)	107, 2474
BanII	GRGCYC	1 cut(s)	424
BbsI	GAAGAC	4 cut(s)	678, 1105, 1898, 2139
Bbv12I	GWGCWC	5 cut(s)	355, 424, 610, 835, 898
BbvCI	CCTCAGC	1 cut(s)	84
BccI	CCATC	9 cut(s)	361, 391, 850, 970, 1093, 1133, 1279, 1937, 2429
BceAI	ACGGC	2 cut(s)	74, 82
BciT130I	CCWGG	8 cut(s)	501, 618, 798, 1416, 1464, 2082, 2470, 2942
BciVI	GTATCC	1 cut(s)	1958
BclI	TGATCA	2 cut(s)	1228, 2287
BcnI	CCSGG	1 cut(s)	1298
BcoDI	GTCTC	8 cut(s)	57, 472, 876, 1343, 2388, 2394, 2871, 3004
BfaI	CTAG	8 cut(s)	320, 825, 882, 1620, 2360, 2667, 2832, 3052
BfmI	CTRYAG	1 cut(s)	2376
BfoI	RGCGCY	1 cut(s)	111
BfrI	CTTAAG	1 cut(s)	1255
BfuAI	ACCTGC	3 cut(s)	364, 422, 1201
BfuI	GTATCC	1 cut(s)	1958
BglI	GCCNNNNNGGC	1 cut(s)	1069
BmcAI	AGTACT	1 cut(s)	2726
Bme1390I	CCNGG	9 cut(s)	501, 618, 798, 1298, 1416, 1464, 2082, 2470, 2942
Bme18I	GGWCC	3 cut(s)	560, 620, 1957
BmeRI	GACNNNNNGTC	1 cut(s)	2386
BmeT110I	CYCGRG	2 cut(s)	2391, 2680
BmgT120I	GGNCC	5 cut(s)	272, 560, 620, 1957, 2987
BmiI	GGNNCC	8 cut(s)	109, 561, 562, 622, 1447, 1563, 2476, 2989
BmrFI	CCNGG	9 cut(s)	501, 618, 798, 1298, 1416, 1464, 2082, 2470, 2942
BmrI	ACTGGG	1 cut(s)	2588
BmsI	GCATC	5 cut(s)	135, 495, 1446, 1850, 1912
BmuI	ACTGGG	1 cut(s)	2588
BpiI	GAAGAC	4 cut(s)	678, 1105, 1898, 2139
BplI	GAGNNNNNCTC	4 cut(s)	469, 501, 2789, 2821
BpmI	CTGGAG	2 cut(s)	438, 1173
Bpu10I	CCTNAGC	1 cut(s)	84
Bpu14I	TTCGAA	2 cut(s)	746, 2130
BpuEI	CTTGAG	8 cut(s)	315, 471, 1596, 1654, 2006, 2728, 2791, 2833
BpuMI	CCSGG	1 cut(s)	1298
Bsa29I	ATCGAT	1 cut(s)	1361
BsaHI	GRCGYC	1 cut(s)	108
BsaI	GGTCTC	3 cut(s)	472, 876, 3004
BsaJI	CCNNGG	8 cut(s)	175, 342, 499, 500, 1067, 1414, 2469, 2941
BsaWI	WCCGGW	1 cut(s)	673
BsaXI	ACNNNNNCTCC	4 cut(s)	537, 567, 2088, 2118
Bse118I	RCCGGY	1 cut(s)	8
Bse21I	CCTNAGG	1 cut(s)	1503
Bse3DI	GCAATG	3 cut(s)	1524, 2155, 2287
BseBI	CCWGG	8 cut(s)	501, 618, 798, 1416, 1464, 2082, 2470, 2942
BseCI	ATCGAT	1 cut(s)	1361
BseDI	CCNNGG	8 cut(s)	175, 342, 499, 500, 1067, 1414, 2469, 2941
BseGI	GGATG	4 cut(s)	150, 510, 1351, 2116
BseMI	GCAATG	3 cut(s)	1524, 2155, 2287
BseRI	GAGGAG	3 cut(s)	474, 603, 2669
BseSI	GKGCMC	5 cut(s)	355, 610, 835, 898, 1768
BseX3I	CGGCCG	1 cut(s)	6
BseYI	CCCAGC	1 cut(s)	2714
BsgI	GTGCAG	1 cut(s)	309
Bsh1236I	CGCG	2 cut(s)	14, 1875
Bsh1285I	CGRYCG	1 cut(s)	9
BshNI	GGYRCC	2 cut(s)	107, 2474
BshVI	ATCGAT	1 cut(s)	1361
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	5 cut(s)	355, 424, 610, 835, 898
BsiHKCI	CYCGRG	2 cut(s)	2391, 2680
BsiSI	CCGG	4 cut(s)	9, 245, 674, 1297
BslFI	GGGAC	7 cut(s)	391, 546, 606, 1232, 1392, 2135, 2331
BsmAI	GTCTC	8 cut(s)	57, 472, 876, 1343, 2388, 2394, 2871, 3004
BsmBI	CGTCTC	2 cut(s)	57, 2394
BsmFI	GGGAC	7 cut(s)	391, 546, 606, 1232, 1392, 2135, 2331
BsmI	GAATGC	4 cut(s)	914, 1443, 2620, 3049
Bso31I	GGTCTC	3 cut(s)	472, 876, 3004
BsoBI	CYCGRG	2 cut(s)	2391, 2680
Bsp119I	TTCGAA	2 cut(s)	746, 2130
Bsp1286I	GDGCHC	6 cut(s)	355, 424, 610, 835, 898, 1768
Bsp19I	CCATGG	1 cut(s)	342
Bsp68I	TCGCGA	1 cut(s)	1875
BspACI	CCGC	7 cut(s)	5, 50, 78, 152, 260, 992, 2625
BspDI	ATCGAT	1 cut(s)	1361
BspFNI	CGCG	2 cut(s)	14, 1875
BspLI	GGNNCC	8 cut(s)	109, 561, 562, 622, 1447, 1563, 2476, 2989
BspMI	ACCTGC	3 cut(s)	364, 422, 1201
BspPI	GGATC	8 cut(s)	30, 80, 162, 421, 2197, 2578, 2727, 2963
BspQI	GCTCTTC	1 cut(s)	2986
BspT104I	TTCGAA	2 cut(s)	746, 2130
BspT107I	GGYRCC	2 cut(s)	107, 2474
BspTI	CTTAAG	1 cut(s)	1255
BspTNI	GGTCTC	3 cut(s)	472, 876, 3004
BsrBI	CCGCTC	1 cut(s)	50
BsrDI	GCAATG	3 cut(s)	1524, 2155, 2287
BsrFI	RCCGGY	1 cut(s)	8
BssAI	RCCGGY	1 cut(s)	8
BssECI	CCNNGG	8 cut(s)	175, 342, 499, 500, 1067, 1414, 2469, 2941
BssNAI	GTATAC	1 cut(s)	1973
BssNI	GRCGYC	1 cut(s)	108
BssT1I	CCWWGG	3 cut(s)	175, 342, 1067
Bst1107I	GTATAC	1 cut(s)	1973
Bst2UI	CCWGG	8 cut(s)	501, 618, 798, 1416, 1464, 2082, 2470, 2942
Bst4CI	ACNGT	8 cut(s)	217, 700, 710, 1130, 2324, 2708, 2735, 2777
Bst6I	CTCTTC	4 cut(s)	1317, 1806, 1854, 2986
BstACI	GRCGYC	1 cut(s)	108
BstAFI	CTTAAG	1 cut(s)	1255
BstBI	TTCGAA	2 cut(s)	746, 2130
BstDSI	CCRYGG	1 cut(s)	342
BstENI	CCTNNNNNAGG	1 cut(s)	486
BstF5I	GGATG	4 cut(s)	150, 510, 1351, 2116
BstFNI	CGCG	2 cut(s)	14, 1875
BstH2I	RGCGCY	1 cut(s)	111
BstHHI	GCGC	2 cut(s)	14, 110
BstMAI	GTCTC	8 cut(s)	57, 472, 876, 1343, 2388, 2394, 2871, 3004
BstMCI	CGRYCG	1 cut(s)	9
BstNI	CCWGG	8 cut(s)	501, 618, 798, 1416, 1464, 2082, 2470, 2942
BstNSI	RCATGY	4 cut(s)	152, 496, 1249, 2339
BstSCI	CCNGG	9 cut(s)	499, 616, 796, 1296, 1414, 1462, 2080, 2468, 2940
BstSFI	CTRYAG	1 cut(s)	2376
BstSLI	GKGCMC	5 cut(s)	355, 610, 835, 898, 1768
BstUI	CGCG	2 cut(s)	14, 1875
BstV2I	GAAGAC	4 cut(s)	678, 1105, 1898, 2139
BstX2I	RGATCY	4 cut(s)	413, 2189, 2570, 2968
BstXI	CCANNNNNNTGG	2 cut(s)	2073, 2894
BstYI	RGATCY	4 cut(s)	413, 2189, 2570, 2968
BstZ17I	GTATAC	1 cut(s)	1973
BstZI	CGGCCG	1 cut(s)	6
Bsu15I	ATCGAT	1 cut(s)	1361
Bsu36I	CCTNAGG	1 cut(s)	1503
BsuI	GTATCC	1 cut(s)	1958
BsuTUI	ATCGAT	1 cut(s)	1361
BtgI	CCRYGG	1 cut(s)	342
BtgZI	GCGATG	1 cut(s)	1347
BtsCI	GGATG	4 cut(s)	150, 510, 1351, 2116
BtuMI	TCGCGA	1 cut(s)	1875
BveI	ACCTGC	3 cut(s)	364, 422, 1201
CaiI	CAGNNNCTG	2 cut(s)	281, 2546
CfoI	GCGC	2 cut(s)	14, 110
Cfr10I	RCCGGY	1 cut(s)	8
Cfr13I	GGNCC	5 cut(s)	272, 560, 620, 1957, 2987
ClaI	ATCGAT	1 cut(s)	1361
CsiI	ACCWGGT	1 cut(s)	1462
Csp6I	GTAC	4 cut(s)	16, 1467, 2725, 2946
CviQI	GTAC	4 cut(s)	16, 1467, 2725, 2946
DinI	GGCGCC	1 cut(s)	109
DraIII	CACNNNGTG	1 cut(s)	911
DrdI	GACNNNNNNGTC	1 cut(s)	2585
DriI	GACNNNNNGTC	1 cut(s)	2386
DseDI	GACNNNNNNGTC	1 cut(s)	2585
EaeI	YGGCCR	7 cut(s)	6, 182, 937, 1070, 2842, 2884, 2938
EagI	CGGCCG	1 cut(s)	6
Eam1104I	CTCTTC	4 cut(s)	1317, 1806, 1854, 2986
Eam1105I	GACNNNNNGTC	1 cut(s)	2386
EarI	CTCTTC	4 cut(s)	1317, 1806, 1854, 2986
Ecl136II	GAGCTC	1 cut(s)	422
EclXI	CGGCCG	1 cut(s)	6
Eco130I	CCWWGG	3 cut(s)	175, 342, 1067
Eco24I	GRGCYC	1 cut(s)	424
Eco31I	GGTCTC	3 cut(s)	472, 876, 3004
Eco32I	GATATC	2 cut(s)	1359, 2407
Eco47I	GGWCC	3 cut(s)	560, 620, 1957
Eco52I	CGGCCG	1 cut(s)	6
Eco53kI	GAGCTC	1 cut(s)	422
Eco57I	CTGAAG	2 cut(s)	25, 1937
Eco81I	CCTNAGG	1 cut(s)	1503
Eco88I	CYCGRG	2 cut(s)	2391, 2680
EcoICRI	GAGCTC	1 cut(s)	422
EcoNI	CCTNNNNNAGG	1 cut(s)	486
EcoO109I	RGGNCCY	1 cut(s)	560
EcoRI	GAATTC	3 cut(s)	748, 1054, 2085
EcoRII	CCWGG	8 cut(s)	499, 616, 796, 1414, 1462, 2080, 2468, 2940
EcoRV	GATATC	2 cut(s)	1359, 2407
EcoT14I	CCWWGG	3 cut(s)	175, 342, 1067
EcoT22I	ATGCAT	1 cut(s)	150
EcoT38I	GRGCYC	1 cut(s)	424
EgeI	GGCGCC	1 cut(s)	109
EheI	GGCGCC	1 cut(s)	109
ErhI	CCWWGG	3 cut(s)	175, 342, 1067
Esp3I	CGTCTC	2 cut(s)	57, 2394
FaqI	GGGAC	7 cut(s)	391, 546, 606, 1232, 1392, 2135, 2331
FbaI	TGATCA	2 cut(s)	1228, 2287
FblI	GTMKAC	3 cut(s)	1125, 1972, 2379
FokI	GGATG	4 cut(s)	157, 517, 1358, 2123
FriOI	GRGCYC	1 cut(s)	424
FspBI	CTAG	8 cut(s)	320, 825, 882, 1620, 2360, 2667, 2832, 3052
GlaI	GCGC	2 cut(s)	13, 109
GsaI	CCCAGC	1 cut(s)	2718
GsuI	CTGGAG	2 cut(s)	438, 1173
HaeII	RGCGCY	1 cut(s)	111
HapII	CCGG	4 cut(s)	9, 245, 674, 1297
HhaI	GCGC	2 cut(s)	14, 110
Hin1I	GRCGYC	1 cut(s)	108
Hin6I	GCGC	2 cut(s)	12, 108
HinP1I	GCGC	2 cut(s)	12, 108
HincII	GTYRAC	4 cut(s)	813, 1373, 1851, 2590
HindII	GTYRAC	4 cut(s)	813, 1373, 1851, 2590
HindIII	AAGCTT	1 cut(s)	33
HpaII	CCGG	4 cut(s)	9, 245, 674, 1297
HphI	GGTGA	4 cut(s)	1005, 1520, 2297, 2308
Hpy99I	CGWCG	1 cut(s)	95
HpyAV	CCTTC	9 cut(s)	735, 939, 1026, 1295, 1961, 2132, 2361, 3001, 3019
HpyCH4III	ACNGT	8 cut(s)	217, 700, 710, 1130, 2324, 2708, 2735, 2777
HpyCH4IV	ACGT	1 cut(s)	2014
HpySE526I	ACGT	1 cut(s)	2014
Hsp92I	GRCGYC	1 cut(s)	108
HspAI	GCGC	2 cut(s)	12, 108
KasI	GGCGCC	1 cut(s)	107
KflI	GGGWCCC	1 cut(s)	560
KroI	GCCGGC	1 cut(s)	8
KroNI	GCCGGC	1 cut(s)	10
Ksp22I	TGATCA	2 cut(s)	1228, 2287
LguI	GCTCTTC	1 cut(s)	2986
LmnI	GCTCC	8 cut(s)	364, 419, 427, 800, 1288, 1567, 1924, 2096
LweI	GCATC	5 cut(s)	135, 495, 1446, 1850, 1912
MabI	ACCWGGT	1 cut(s)	1462
MaeI	CTAG	8 cut(s)	320, 825, 882, 1620, 2360, 2667, 2832, 3052
MaeII	ACGT	1 cut(s)	2014
MaeIII	GTNAC	5 cut(s)	211, 994, 1130, 1147, 1292
MbiI	CCGCTC	1 cut(s)	50
MfeI	CAATTG	1 cut(s)	1911
MflI	RGATCY	4 cut(s)	413, 2189, 2570, 2968
MhlI	GDGCHC	6 cut(s)	355, 424, 610, 835, 898, 1768
MlsI	TGGCCA	5 cut(s)	939, 1072, 2844, 2886, 2940
MluNI	TGGCCA	5 cut(s)	939, 1072, 2844, 2886, 2940
Mly113I	GGCGCC	1 cut(s)	108
MlyI	GAGTC	9 cut(s)	1414, 1661, 2177, 2495, 2531, 2666, 2906, 2957, 3019
MmeI	TCCRAC	5 cut(s)	1009, 1015, 1163, 1323, 2090
Mox20I	TGGCCA	5 cut(s)	939, 1072, 2844, 2886, 2940
Mph1103I	ATGCAT	1 cut(s)	150
MroNI	GCCGGC	1 cut(s)	8
MroXI	GAANNNNTTC	4 cut(s)	449, 634, 2272, 3047
MscI	TGGCCA	5 cut(s)	939, 1072, 2844, 2886, 2940
MseI	TTAA	6 cut(s)	239, 306, 702, 1256, 1745, 1917
MslI	CAYNNNNRTG	3 cut(s)	1700, 2295, 2301
Msp20I	TGGCCA	5 cut(s)	939, 1072, 2844, 2886, 2940
MspA1I	CMGCKG	5 cut(s)	78, 278, 989, 2048, 2102
MspCI	CTTAAG	1 cut(s)	1255
MspI	CCGG	4 cut(s)	9, 245, 674, 1297
MspR9I	CCNGG	9 cut(s)	501, 618, 798, 1298, 1416, 1464, 2082, 2470, 2942
MunI	CAATTG	1 cut(s)	1911
Mva1269I	GAATGC	4 cut(s)	914, 1443, 2620, 3049
MvaI	CCWGG	8 cut(s)	501, 618, 798, 1416, 1464, 2082, 2470, 2942
MvnI	CGCG	2 cut(s)	14, 1875
NaeI	GCCGGC	1 cut(s)	10
NarI	GGCGCC	1 cut(s)	108
NciI	CCSGG	1 cut(s)	1298
NcoI	CCATGG	1 cut(s)	342
NgoMIV	GCCGGC	1 cut(s)	8
NlaIV	GGNNCC	8 cut(s)	109, 561, 562, 622, 1447, 1563, 2476, 2989
NmuCI	GTSAC	3 cut(s)	211, 1130, 1147
NruI	TCGCGA	1 cut(s)	1875
NsiI	ATGCAT	1 cut(s)	150
NspI	RCATGY	4 cut(s)	152, 496, 1249, 2339
NspV	TTCGAA	2 cut(s)	746, 2130
PaeI	GCATGC	3 cut(s)	152, 496, 1249
PaeR7I	CTCGAG	2 cut(s)	2391, 2680
PaqCI	CACCTGC	2 cut(s)	364, 1201
PasI	CCCWGGG	1 cut(s)	500
PciI	ACATGT	1 cut(s)	2335
PciSI	GCTCTTC	1 cut(s)	2986
PctI	GAATGC	4 cut(s)	914, 1443, 2620, 3049
PdiI	GCCGGC	1 cut(s)	10
PdmI	GAANNNNTTC	4 cut(s)	449, 634, 2272, 3047
PflMI	CCANNNNNTGG	1 cut(s)	1421
PleI	GAGTC	9 cut(s)	1413, 1661, 2177, 2495, 2531, 2665, 2906, 2957, 3018
PluTI	GGCGCC	1 cut(s)	111
PpsI	GAGTC	9 cut(s)	1413, 1661, 2177, 2495, 2531, 2665, 2906, 2957, 3018
PpuMI	RGGWCCY	1 cut(s)	560
PscI	ACATGT	1 cut(s)	2335
PshBI	ATTAAT	1 cut(s)	306
PsiI	TTATAA	1 cut(s)	195
Psp124BI	GAGCTC	1 cut(s)	424
Psp1406I	AACGTT	1 cut(s)	2014
Psp5II	RGGWCCY	1 cut(s)	560
Psp6I	CCWGG	8 cut(s)	499, 616, 796, 1414, 1462, 2080, 2468, 2940
PspFI	CCCAGC	1 cut(s)	2714
PspGI	CCWGG	8 cut(s)	499, 616, 796, 1414, 1462, 2080, 2468, 2940
PspN4I	GGNNCC	8 cut(s)	109, 561, 562, 622, 1447, 1563, 2476, 2989
PspPI	GGNCC	5 cut(s)	272, 560, 620, 1957, 2987
PspPPI	RGGWCCY	1 cut(s)	560
PspXI	VCTCGAGB	1 cut(s)	2680
PstNI	CAGNNNCTG	2 cut(s)	281, 2546
PsuI	RGATCY	4 cut(s)	413, 2189, 2570, 2968
PvuII	CAGCTG	4 cut(s)	278, 989, 2048, 2102
RruI	TCGCGA	1 cut(s)	1875
RsaI	GTAC	4 cut(s)	17, 1468, 2726, 2947
RsaNI	GTAC	4 cut(s)	16, 1467, 2725, 2946
RseI	CAYNNNNRTG	3 cut(s)	1700, 2295, 2301
SacI	GAGCTC	1 cut(s)	424
SapI	GCTCTTC	1 cut(s)	2986
SaqAI	TTAA	6 cut(s)	239, 306, 702, 1256, 1745, 1917
Sau96I	GGNCC	5 cut(s)	272, 560, 620, 1957, 2987
ScaI	AGTACT	1 cut(s)	2726
SchI	GAGTC	9 cut(s)	1414, 1661, 2177, 2495, 2531, 2666, 2906, 2957, 3019
ScrFI	CCNGG	9 cut(s)	501, 618, 798, 1298, 1416, 1464, 2082, 2470, 2942
SduI	GDGCHC	6 cut(s)	355, 424, 610, 835, 898, 1768
SexAI	ACCWGGT	1 cut(s)	1462
SfaNI	GCATC	5 cut(s)	135, 495, 1446, 1850, 1912
SfcI	CTRYAG	1 cut(s)	2376
SfoI	GGCGCC	1 cut(s)	109
Sfr274I	CTCGAG	2 cut(s)	2391, 2680
SfuI	TTCGAA	2 cut(s)	746, 2130
SinI	GGWCC	3 cut(s)	560, 620, 1957
SlaI	CTCGAG	2 cut(s)	2391, 2680
SmiMI	CAYNNNNRTG	3 cut(s)	1700, 2295, 2301
SphI	GCATGC	3 cut(s)	152, 496, 1249
SsiI	CCGC	7 cut(s)	5, 50, 78, 152, 260, 992, 2625
SspDI	GGCGCC	1 cut(s)	107
SspMI	CTAG	8 cut(s)	320, 825, 882, 1620, 2360, 2667, 2832, 3052
SstI	GAGCTC	1 cut(s)	424
StyD4I	CCNGG	9 cut(s)	499, 616, 796, 1296, 1414, 1462, 2080, 2468, 2940
StyI	CCWWGG	3 cut(s)	175, 342, 1067
TaaI	ACNGT	8 cut(s)	217, 700, 710, 1130, 2324, 2708, 2735, 2777
TaiI	ACGT	1 cut(s)	2017
TatI	WGTACW	1 cut(s)	2724
TauI	GCSGC	2 cut(s)	8, 263
Tru1I	TTAA	6 cut(s)	239, 306, 702, 1256, 1745, 1917
Tru9I	TTAA	6 cut(s)	239, 306, 702, 1256, 1745, 1917
TseFI	GTSAC	3 cut(s)	211, 1130, 1147
Tsp45I	GTSAC	3 cut(s)	211, 1130, 1147
TspGWI	ACGGA	5 cut(s)	35, 75, 1772, 1949, 2109
Van91I	CCANNNNNTGG	1 cut(s)	1421
Vha464I	CTTAAG	1 cut(s)	1255
VneI	GTGCAC	4 cut(s)	351, 606, 831, 894
VpaK11BI	GGWCC	3 cut(s)	560, 620, 1957
VspI	ATTAAT	1 cut(s)	306
XagI	CCTNNNNNAGG	1 cut(s)	486
XapI	RAATTY	8 cut(s)	188, 748, 1054, 1168, 1537, 2085, 2268, 2422
XceI	RCATGY	4 cut(s)	152, 496, 1249, 2339
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1418, 1577
XhoI	CTCGAG	2 cut(s)	2391, 2680
XmiI	GTMKAC	3 cut(s)	1125, 1972, 2379
XmnI	GAANNNNTTC	4 cut(s)	449, 634, 2272, 3047
XspI	CTAG	8 cut(s)	320, 825, 882, 1620, 2360, 2667, 2832, 3052
ZrmI	AGTACT	1 cut(s)	2726
Zsp2I	ATGCAT	1 cut(s)	150

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.