AT1G22310

Methyl-CpG binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006195
Physical Location & Seq
Reverse (-)
7881503 .. 7883824
2322 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G22310.1

Sequence Viewer

Length: 1278 bp
ATGAGTCCGCTGGTAGCCGGAAACAGACATTTCTCCGTCTCCGCCTTGCGCGCCACCCTCAACCACCGGAGGAACCTCCTTCGCCGCAACGTCAGAGAGACGATTCTTCCCGTGAAGAACAAACGCAAGTCGCGTCGCTTCTCAGATCTCTGTTCAATGTCGATTCGAATCAGAGTAAAGAGGAAGAAGACGAAGGAGAGGAAGAGCTCGAAGATAACGAAGGGAACCTCCGGGAATAAAATAAAGAGGAAGCGTGGACGGCCGAGGAAGATTCGGAACCCTAGCGAAGAGAATGAGGTTTTGGATTTAACTGGTGAAGCATCTACGTATGTGTTTGTAGATAAAACTAGTAGCAATTTGGGAATGGTGAGTAGAGTTGGTAGTTCAGGAATCTCCCTGGATTCAAATTCAGTGAAGAGAAAGAGAGGTCGTCCTCCAAAGAATAAGGAAGAGATAATGAACCTAGAGAAGAGAGACAGTGCAATTGTGAATATTTCTGCGTTTGATAAGGAAGAGTTAGTAGTGAACTTGGAGAACAGAGAAGGTACAATTGTGGATCTCTCTGCATTGGCTAGTGTTTCAGAGGATCCATACGAAGAGGAGCTGAGGAGGATCACCGTTGGGTTGAAGACAAAGGAAGAGATCTTAGGTTTCTTGGAACAATTGAATGGTGAATGGGTGAATATAGGGAAGAAGAAGAAAGTTGTGAATGCTTGTGATTATGGAGGATACTTGCCTAGAGGATGGAGACTTATGCTCTACATCAAAAGGAAAGGTAGCAATTTGTTGCTGGCTTGTCGCAGATACATAAGCCCTGATGGGCAACAGTTTGAAACCTGCAAGGAAGTCTCCACATATCTACGATCACTTCTTGAATCCCCAAGCAAGAACCAACATTATTACTTGCAATCTGACAACAAGACTCTAGGACAACAACCAGTGATAGCTAACGAATCTTTACTAGGTAACTCTGATTCCATGGACTCTGAGACAATGCAGTACCTTGAGAGTGGAAGAACCAGCAGTGAGGTGTTTGAGGAAGCAAAAGCCGTAGAGAATGGCAATGAAGCAGATCGAGTGAAAACCAGTCTGATGCAGAAAGATGATAATGCAGATTTTCTGAATGGAGTTGAGGACAACGACGATGATATGAAGAAAAGAGATGGTAACATGGAGAATTTGGCGACTCTATCGAATTCAGAAATGACGAAAAGCCTACCGACAACGACTAATGAGCTTCAACAGTATTTCAGCTCACAAATCAACCGAGTTCAGTGA

Protein Analysis

425

Amino Acids

48.26

Weight (kDa)

9.55

Isoelectric Point (pI)

56.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBD PF01429 245 - 289 2.8e-07 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 845
AccII CGCG 2 cut(s) 51, 133
AciI CCGC 3 cut(s) 8, 42, 85
AclWI GGATC 4 cut(s) 564, 581, 594, 620
AcoI YGGCCR 1 cut(s) 260
AcsI RAATTY 3 cut(s) 406, 1177, 1195
AfaI GTAC 2 cut(s) 547, 1001
AgsI TTSAA 7 cut(s) 156, 405, 628, 667, 833, 875, 1241
AhlI ACTAGT 1 cut(s) 347
AjnI CCWGG 1 cut(s) 396
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 5 cut(s) 207, 604, 947, 1237, 1254
AluI AGCT 5 cut(s) 207, 604, 947, 1237, 1254
Alw21I GWGCWC 1 cut(s) 209
Alw26I GTCTC 6 cut(s) 43, 92, 468, 742, 853, 983
AlwI GGATC 4 cut(s) 564, 581, 594, 620
AoxI GGCC 1 cut(s) 260
ApoI RAATTY 3 cut(s) 406, 1177, 1195
AspLEI GCGC 2 cut(s) 51, 53
AsuC2I CCSGG 1 cut(s) 232
AsuHPI GGTGA 5 cut(s) 326, 379, 607, 683, 691
AsuII TTCGAA 1 cut(s) 166
BaeI ACNNNNGTAYC 2 cut(s) 537, 570
BamHI GGATCC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 209
BbsI GAAGAC 2 cut(s) 194, 635
Bbv12I GWGCWC 1 cut(s) 209
BbvCI CCTCAGC 1 cut(s) 605
BccI CCATC 3 cut(s) 738, 812, 1157
BceAI ACGGC 2 cut(s) 275, 1034
BciT130I CCWGG 1 cut(s) 398
BciVI GTATCC 1 cut(s) 722
BcnI CCSGG 1 cut(s) 232
BcoDI GTCTC 6 cut(s) 43, 92, 468, 742, 853, 983
BcuI ACTAGT 1 cut(s) 347
BfaI CTAG 7 cut(s) 282, 348, 464, 573, 738, 926, 962
BfuAI ACCTGC 1 cut(s) 845
BfuI GTATCC 1 cut(s) 722
BglII AGATCT 2 cut(s) 145, 642
BisI GCNGC 1 cut(s) 85
BlsI GCNGC 1 cut(s) 86
Bme1390I CCNGG 2 cut(s) 232, 398
BmiI GGNNCC 4 cut(s) 74, 226, 278, 588
BmrFI CCNGG 2 cut(s) 232, 398
BmsI GCATC 2 cut(s) 329, 1083
BpiI GAAGAC 2 cut(s) 194, 635
Bpu10I CCTNAGC 1 cut(s) 605
Bpu14I TTCGAA 1 cut(s) 166
BpuEI CTTGAG 1 cut(s) 1025
BpuMI CCSGG 1 cut(s) 232
BsaAI YACGTR 1 cut(s) 327
BsaBI GATNNNNATC 1 cut(s) 167
BsaJI CCNNGG 3 cut(s) 263, 396, 978
BsaWI WCCGGW 1 cut(s) 66
Bse1I ACTGG 3 cut(s) 316, 938, 1086
Bse3DI GCAATG 1 cut(s) 1069
Bse8I GATNNNNATC 1 cut(s) 167
BseBI CCWGG 1 cut(s) 398
BseDI CCNNGG 3 cut(s) 263, 396, 978
BseGI GGATG 1 cut(s) 749
BseJI GATNNNNATC 1 cut(s) 167
BseMI GCAATG 1 cut(s) 1069
BseMII CTCAG 3 cut(s) 156, 596, 978
BseNI ACTGG 3 cut(s) 316, 938, 1086
BsePI GCGCGC 1 cut(s) 49
BseRI GAGGAG 2 cut(s) 614, 622
BseX3I CGGCCG 1 cut(s) 260
Bsh1236I CGCG 2 cut(s) 51, 133
Bsh1285I CGRYCG 1 cut(s) 263
BshFI GGCC 1 cut(s) 262
BsiEI CGRYCG 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 209
BsiSI CCGG 3 cut(s) 18, 67, 231
BsmAI GTCTC 6 cut(s) 43, 92, 468, 742, 853, 983
BsmBI CGTCTC 2 cut(s) 43, 92
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 1 cut(s) 262
Bsp119I TTCGAA 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 7 cut(s) 145, 556, 586, 612, 642, 863, 1072
Bsp19I CCATGG 1 cut(s) 978
BspACI CCGC 3 cut(s) 8, 42, 85
BspANI GGCC 1 cut(s) 262
BspCNI CTCAG 3 cut(s) 155, 597, 979
BspFNI CGCG 2 cut(s) 51, 133
BspLI GGNNCC 4 cut(s) 74, 226, 278, 588
BspMI ACCTGC 1 cut(s) 845
BspPI GGATC 4 cut(s) 564, 581, 594, 620
BspQI GCTCTTC 1 cut(s) 197
BspT104I TTCGAA 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 1069
BsrI ACTGG 3 cut(s) 316, 938, 1086
BssECI CCNNGG 3 cut(s) 263, 396, 978
BssHII GCGCGC 1 cut(s) 49
BssMI GATC 7 cut(s) 145, 556, 586, 612, 642, 863, 1072
BssT1I CCWWGG 1 cut(s) 978
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 4 cut(s) 479, 619, 828, 1245
Bst6I CTCTTC 8 cut(s) 197, 282, 410, 444, 464, 507, 591, 633
BstBAI YACGTR 1 cut(s) 327
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 2 cut(s) 51, 792
BstDEI CTNAG 4 cut(s) 142, 605, 646, 987
BstDSI CCRYGG 1 cut(s) 978
BstF5I GGATG 1 cut(s) 749
BstFNI CGCG 2 cut(s) 51, 133
BstHHI GCGC 2 cut(s) 51, 53
BstKTI GATC 7 cut(s) 148, 559, 589, 615, 645, 866, 1075
BstMAI GTCTC 6 cut(s) 43, 92, 468, 742, 853, 983
BstMBI GATC 7 cut(s) 145, 556, 586, 612, 642, 863, 1072
BstMCI CGRYCG 1 cut(s) 263
BstMWI GCNNNNNNNGC 2 cut(s) 50, 259
BstNI CCWGG 1 cut(s) 398
BstSCI CCNGG 2 cut(s) 230, 396
BstSNI TACGTA 1 cut(s) 327
BstUI CGCG 2 cut(s) 51, 133
BstV2I GAAGAC 2 cut(s) 194, 635
BstX2I RGATCY 4 cut(s) 145, 556, 586, 642
BstYI RGATCY 4 cut(s) 145, 556, 586, 642
BstZI CGGCCG 1 cut(s) 260
BsuI GTATCC 1 cut(s) 722
BsuRI GGCC 1 cut(s) 262
BtgI CCRYGG 1 cut(s) 978
BtsCI GGATG 1 cut(s) 749
BtsI GCAGTG 1 cut(s) 1030
BtsIMutI CAGTG 4 cut(s) 417, 484, 945, 1030
BveI ACCTGC 1 cut(s) 845
Cac8I GCNNGC 2 cut(s) 51, 792
CfoI GCGC 2 cut(s) 51, 53
CseI GACGC 1 cut(s) 122
Csp6I GTAC 2 cut(s) 546, 1000
CviAII CATG 2 cut(s) 979, 1171
CviQI GTAC 2 cut(s) 546, 1000
DdeI CTNAG 4 cut(s) 142, 605, 646, 987
DpnI GATC 7 cut(s) 147, 558, 588, 614, 644, 865, 1074
DpnII GATC 7 cut(s) 145, 556, 586, 612, 642, 863, 1072
EaeI YGGCCR 1 cut(s) 260
EagI CGGCCG 1 cut(s) 260
Eam1104I CTCTTC 8 cut(s) 197, 282, 410, 444, 464, 507, 591, 633
EarI CTCTTC 8 cut(s) 197, 282, 410, 444, 464, 507, 591, 633
EciI GGCGGA 1 cut(s) 31
Ecl136II GAGCTC 1 cut(s) 207
EclXI CGGCCG 1 cut(s) 260
Eco105I TACGTA 1 cut(s) 327
Eco130I CCWWGG 1 cut(s) 978
Eco24I GRGCYC 1 cut(s) 209
Eco52I CGGCCG 1 cut(s) 260
Eco53kI GAGCTC 1 cut(s) 207
EcoICRI GAGCTC 1 cut(s) 207
EcoRI GAATTC 1 cut(s) 1195
EcoRII CCWGG 1 cut(s) 396
EcoT14I CCWWGG 1 cut(s) 978
EcoT38I GRGCYC 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 978
Esp3I CGTCTC 2 cut(s) 43, 92
FaeI CATG 2 cut(s) 982, 1174
FatI CATG 2 cut(s) 978, 1170
Fnu4HI GCNGC 1 cut(s) 85
FokI GGATG 1 cut(s) 756
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 1 cut(s) 85
FspBI CTAG 7 cut(s) 282, 348, 464, 573, 738, 926, 962
GlaI GCGC 2 cut(s) 50, 52
GluI GCNGC 1 cut(s) 85
HaeIII GGCC 1 cut(s) 262
HapII CCGG 3 cut(s) 18, 67, 231
HgaI GACGC 1 cut(s) 122
HhaI GCGC 2 cut(s) 51, 53
Hin1II CATG 2 cut(s) 982, 1174
Hin6I GCGC 2 cut(s) 49, 51
HinP1I GCGC 2 cut(s) 49, 51
HpaII CCGG 3 cut(s) 18, 67, 231
HphI GGTGA 5 cut(s) 326, 379, 607, 683, 691
Hpy166II GTNNAC 2 cut(s) 257, 526
Hpy188III TCNNGA 2 cut(s) 387, 872
Hpy8I GTNNAC 2 cut(s) 257, 526
Hpy99I CGWCG 2 cut(s) 138, 1145
HpyAV CCTTC 4 cut(s) 89, 187, 214, 536
HpyCH4III ACNGT 4 cut(s) 479, 619, 828, 1245
HpyCH4IV ACGT 2 cut(s) 90, 326
HpyCH4V TGCA 7 cut(s) 482, 566, 840, 907, 997, 1096, 1112
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 259
HpyF3I CTNAG 4 cut(s) 142, 605, 646, 987
HpySE526I ACGT 2 cut(s) 90, 326
Hsp92II CATG 2 cut(s) 982, 1174
HspAI GCGC 2 cut(s) 49, 51
Kzo9I GATC 7 cut(s) 145, 556, 586, 612, 642, 863, 1072
LguI GCTCTTC 1 cut(s) 197
LmnI GCTCC 1 cut(s) 601
LweI GCATC 2 cut(s) 329, 1083
MaeI CTAG 7 cut(s) 282, 348, 464, 573, 738, 926, 962
MaeII ACGT 2 cut(s) 90, 326
MaeIII GTNAC 2 cut(s) 965, 1166
MalI GATC 7 cut(s) 147, 558, 588, 614, 644, 865, 1074
MboI GATC 7 cut(s) 145, 556, 586, 612, 642, 863, 1072
MfeI CAATTG 3 cut(s) 483, 549, 662
MflI RGATCY 4 cut(s) 145, 556, 586, 642
MhlI GDGCHC 1 cut(s) 209
MluCI AATT 8 cut(s) 355, 406, 483, 549, 662, 781, 1177, 1195
MlyI GAGTC 4 cut(s) 13, 916, 977, 1180
MseI TTAA 1 cut(s) 308
MspA1I CMGCKG 1 cut(s) 10
MspI CCGG 3 cut(s) 18, 67, 231
MspR9I CCNGG 2 cut(s) 232, 398
MunI CAATTG 3 cut(s) 483, 549, 662
Mva1269I GAATGC 1 cut(s) 715
MvaI CCWGG 1 cut(s) 398
MvnI CGCG 2 cut(s) 51, 133
MwoI GCNNNNNNNGC 2 cut(s) 50, 259
NciI CCSGG 1 cut(s) 232
NcoI CCATGG 1 cut(s) 978
NdeII GATC 7 cut(s) 145, 556, 586, 612, 642, 863, 1072
NlaIII CATG 2 cut(s) 982, 1174
NlaIV GGNNCC 4 cut(s) 74, 226, 278, 588
NmeAIII GCCGAG 1 cut(s) 288
NspV TTCGAA 1 cut(s) 166
PauI GCGCGC 1 cut(s) 49
PciSI GCTCTTC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 2 cut(s) 130, 215
PctI GAATGC 1 cut(s) 715
PfeI GAWTC 9 cut(s) 103, 163, 168, 271, 390, 401, 875, 953, 974
PfoI TCCNGGA 1 cut(s) 230
PkrI GCNGC 1 cut(s) 86
PleI GAGTC 4 cut(s) 12, 916, 977, 1180
PpsI GAGTC 4 cut(s) 12, 916, 977, 1180
Ppu21I YACGTR 1 cut(s) 327
Psp124BI GAGCTC 1 cut(s) 209
Psp6I CCWGG 1 cut(s) 396
PspGI CCWGG 1 cut(s) 396
PspN4I GGNNCC 4 cut(s) 74, 226, 278, 588
PsuI RGATCY 4 cut(s) 145, 556, 586, 642
PteI GCGCGC 1 cut(s) 49
RsaI GTAC 2 cut(s) 547, 1001
RsaNI GTAC 2 cut(s) 546, 1000
SacI GAGCTC 1 cut(s) 209
SapI GCTCTTC 1 cut(s) 197
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 1 cut(s) 85
Sau3AI GATC 7 cut(s) 145, 556, 586, 612, 642, 863, 1072
SchI GAGTC 4 cut(s) 13, 916, 977, 1180
ScrFI CCNGG 2 cut(s) 232, 398
SduI GDGCHC 1 cut(s) 209
SfaNI GCATC 2 cut(s) 329, 1083
SfuI TTCGAA 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 1004
SmoI CTYRAG 1 cut(s) 1004
SnaBI TACGTA 1 cut(s) 327
SpeI ACTAGT 1 cut(s) 347
Sse9I AATT 8 cut(s) 355, 406, 483, 549, 662, 781, 1177, 1195
SsiI CCGC 3 cut(s) 8, 42, 85
SspI AATATT 1 cut(s) 493
SspMI CTAG 7 cut(s) 282, 348, 464, 573, 738, 926, 962
SstI GAGCTC 1 cut(s) 209
StyD4I CCNGG 2 cut(s) 230, 396
StyI CCWWGG 1 cut(s) 978
TaaI ACNGT 4 cut(s) 479, 619, 828, 1245
TaiI ACGT 2 cut(s) 93, 329
TaqI TCGA 5 cut(s) 161, 166, 209, 1075, 1193
TasI AATT 8 cut(s) 355, 406, 483, 549, 662, 781, 1177, 1195
TauI GCSGC 1 cut(s) 87
TfiI GAWTC 9 cut(s) 103, 163, 168, 271, 390, 401, 875, 953, 974
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 4 cut(s) 417, 484, 945, 1030
TspDTI ATGAA 3 cut(s) 473, 1080, 1166
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 4 cut(s) 417, 484, 945, 1030
XapI RAATTY 3 cut(s) 406, 1177, 1195
XspI CTAG 7 cut(s) 282, 348, 464, 573, 738, 926, 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.