Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008036

Physical Location & Seq

Genomic Coordinates Forward (+)

8942713 .. 8944442

Gene Structure 1730 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G25460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 960 bp

ATGGCAGAGTACTTGGTTACCGGAGGAACCAGCTTTATAGCCTCTCATGTTATCAAGTCACTTCTAGAATTTGGTCATTACGTAAGAACAACTGTTAGAGACTCAGATGAAGAAAAGGTGGGTTTCTTGTGGGACTTAAAAGGAGCAAAAGAAAGGTTGAAGATCTTCGAAGCTGATCTAACGATAGAGGGAAGCTTTGACGAAGCAGTCAACGGTGTTGATGGAGTCTTCCACATCGCATCTCGAGTTTCTGTTCGTCTGGACAACAACAATCTGGATAAATTTGATCCTAATATAAGTGGAACAATGAATGTGATGAACTCTTGTGCAAAGTCAAGAAACACTGTGAAAAGGATTGTTCTCACATCTTCTTCAACCGCGATACGGTACCGTTTTGATGCTACACAAGTCTCTCCACTTAATGAATCGCATTGGACCGATCTTGAATACTGCAAACACTTCAAAATTTGGTATGCTTATAAAAAGACTTTAGGGGAGAAAGAAGCTTGGAGGATCGCAGCTGATAAGAAGCTAAACCTAGTTGTCGTAATCCCATCATTTTGTATTGGTCCAATACTTAGTCCAAAACCTACAAGCTCCCCTCTCATTTTTCTGTCCATTATCAAAGGGACTCGTGGAACGTACCCTAACTTCAGGGGAGGGTTTGTGCACATAGACGATGTAGTTGCTGCACAAATCTTAGCAATGGAAGAACCTAAAGCATCAGGAAGAATCTTATGTTCGAGTTCAGTGGCTCACTGGTCAGAGATCATTGAGATGCTACGAATCAAATACCCATTATACCCATTTGAGACCAAGTGTGGCAGTGAAGAAGGGAAAGATATGCCTCATAGCTTAGACACAACAAAAATACATGAACTTGGCTTTGCCTCCTTCAAGTCATTAACAGAGATGTTTGATGACTGTATCAAGTGTTTTCAAGACAAGGGTTTGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006725 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0016043 GO:0019438 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0043062 GO:0044085 GO:0044237 GO:0044249 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0071840 GO:0080110 GO:0085029

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Epimerase

Protein Analysis

319

Amino Acids

35.83

Weight (kDa)

6.76

Isoelectric Point (pI)

33.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	5 - 242	2.7e-22	NAD dependent epimerase/dehydratase family
3Beta_HSD	PF01073	5 - 167	1e-15	3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd	PF16363	5 - 143	1.7e-11	GDP-mannose 4,6 dehydratase
Polysacc_synt_2	PF02719	5 - 129	7.9e-07	Polysaccharide biosynthesis protein
NmrA	PF05368	5 - 85	3.6e-06	NmrA-like family
NAD_binding_4	PF07993	6 - 197	4.7e-11	Male sterility protein
NAD_binding_10	PF13460	8 - 127	2e-09	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008036)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G25460 AT1G68540 AT1G68540
fragaria_vesca	FvH4_4g27430 FvH4_4g27430
malus_domestica	MD13G1042500.v1.1 MD16G1043400.v1.1
prunus_persica	Prupe.1G310300_v2.0.a1
pyrus_communis	pycom13g03700 pycom13g03710 pycom13g03720 pycom16g03750
rosa_chinensis	RchiOBHm_Chr4g0435251
rosa_laevigata	RLG00000006604
rosa_multiflora	Rmu_sc0002132.1_g000069
rosa_roxburghii	Rroxscaffold_5G00376540
rosa_rugosa	Rorug04G0284000 Rorug04G0284100
rosa_samantha	Rh4AG339300 Rh4BG347700 Rh4CG362300 Rh4CG362400 Rh4DG341800
rosa_wichuraiana	Rw4G029600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	480
AasI	GACNNNNNNGTC	1 cut(s)	206
Acc65I	GGTACC	1 cut(s)	387
AccB1I	GGYRCC	1 cut(s)	387
AccII	CGCG	1 cut(s)	380
AciI	CCGC	1 cut(s)	378
AclWI	GGATC	2 cut(s)	281, 521
AcsI	RAATTY	3 cut(s)	68, 281, 465
AcuI	CTGAAG	1 cut(s)	637
AfaI	GTAC	3 cut(s)	11, 389, 644
AfiI	CCNNNNNNNGG	1 cut(s)	384
AgsI	TTSAA	6 cut(s)	160, 375, 446, 463, 898, 941
AluBI	AGCT	8 cut(s)	33, 173, 195, 506, 521, 532, 597, 855
AluI	AGCT	8 cut(s)	33, 173, 195, 506, 521, 532, 597, 855
Alw21I	GWGCWC	1 cut(s)	672
Alw26I	GTCTC	3 cut(s)	93, 415, 806
Alw44I	GTGCAC	1 cut(s)	668
AlwI	GGATC	2 cut(s)	281, 521
Ama87I	CYCGRG	1 cut(s)	243
ApaLI	GTGCAC	1 cut(s)	668
ApeKI	GCWGC	2 cut(s)	518, 689
ApoI	RAATTY	3 cut(s)	68, 281, 465
Asp700I	GAANNNNTTC	1 cut(s)	164
Asp718I	GGTACC	1 cut(s)	387
AspS9I	GGNCC	2 cut(s)	435, 569
AsuII	TTCGAA	1 cut(s)	168
AvaI	CYCGRG	1 cut(s)	243
AvaII	GGWCC	2 cut(s)	435, 569
BaeGI	GKGCMC	1 cut(s)	672
BanI	GGYRCC	1 cut(s)	387
BarI	GAAGNNNNNNTAC	2 cut(s)	635, 667
BauI	CACGAG	1 cut(s)	633
BbsI	GAAGAC	1 cut(s)	220
Bbv12I	GWGCWC	1 cut(s)	672
BbvI	GCAGC	2 cut(s)	530, 676
BccI	CCATC	2 cut(s)	215, 562
BcgI	CGANNNNNNTGC	2 cut(s)	668, 702
BcoDI	GTCTC	3 cut(s)	93, 415, 806
BfaI	CTAG	2 cut(s)	65, 539
BglII	AGATCT	1 cut(s)	162
BisI	GCNGC	2 cut(s)	519, 690
BlsI	GCNGC	2 cut(s)	520, 691
BmcAI	AGTACT	1 cut(s)	11
Bme18I	GGWCC	2 cut(s)	435, 569
BmeT110I	CYCGRG	1 cut(s)	243
BmgT120I	GGNCC	2 cut(s)	435, 569
BmiI	GGNNCC	2 cut(s)	28, 389
BmsI	GCATC	4 cut(s)	248, 388, 731, 768
BpiI	GAAGAC	1 cut(s)	220
Bpu14I	TTCGAA	1 cut(s)	168
BsaAI	YACGTR	1 cut(s)	82
BsaI	GGTCTC	1 cut(s)	806
BsaWI	WCCGGW	1 cut(s)	20
BsaXI	ACNNNNNCTCC	2 cut(s)	651, 681
Bsc4I	CCNNNNNNNGG	1 cut(s)	384
Bse1I	ACTGG	1 cut(s)	764
Bse3DI	GCAATG	1 cut(s)	711
BseLI	CCNNNNNNNGG	1 cut(s)	384
BseMI	GCAATG	1 cut(s)	711
BseMII	CTCAG	1 cut(s)	117
BseNI	ACTGG	1 cut(s)	764
BseSI	GKGCMC	1 cut(s)	672
BseXI	GCAGC	2 cut(s)	530, 676
BsgI	GTGCAG	1 cut(s)	675
Bsh1236I	CGCG	1 cut(s)	380
BshNI	GGYRCC	1 cut(s)	387
BsiHKAI	GWGCWC	1 cut(s)	672
BsiHKCI	CYCGRG	1 cut(s)	243
BsiSI	CCGG	1 cut(s)	21
BslFI	GGGAC	2 cut(s)	146, 643
BslI	CCNNNNNNNGG	1 cut(s)	384
BsmAI	GTCTC	3 cut(s)	93, 415, 806
BsmFI	GGGAC	2 cut(s)	146, 643
Bso31I	GGTCTC	1 cut(s)	806
BsoBI	CYCGRG	1 cut(s)	243
Bsp119I	TTCGAA	1 cut(s)	168
Bsp1286I	GDGCHC	1 cut(s)	672
Bsp143I	GATC	6 cut(s)	162, 175, 286, 439, 513, 768
BspACI	CCGC	1 cut(s)	378
BspCNI	CTCAG	1 cut(s)	116
BspFNI	CGCG	1 cut(s)	380
BspLI	GGNNCC	2 cut(s)	28, 389
BspPI	GGATC	2 cut(s)	281, 521
BspT104I	TTCGAA	1 cut(s)	168
BspT107I	GGYRCC	1 cut(s)	387
BspTNI	GGTCTC	1 cut(s)	806
BsrDI	GCAATG	1 cut(s)	711
BsrI	ACTGG	1 cut(s)	764
BssMI	GATC	6 cut(s)	162, 175, 286, 439, 513, 768
BssSI	CACGAG	1 cut(s)	633
Bst2BI	CACGAG	1 cut(s)	633
Bst4CI	ACNGT	6 cut(s)	94, 215, 346, 387, 392, 926
BstBAI	YACGTR	1 cut(s)	82
BstBI	TTCGAA	1 cut(s)	168
BstDEI	CTNAG	4 cut(s)	103, 578, 700, 856
BstEII	GGTNACC	1 cut(s)	16
BstFNI	CGCG	1 cut(s)	380
BstKTI	GATC	6 cut(s)	165, 178, 289, 442, 516, 771
BstMAI	GTCTC	3 cut(s)	93, 415, 806
BstMBI	GATC	6 cut(s)	162, 175, 286, 439, 513, 768
BstPI	GGTNACC	1 cut(s)	16
BstSLI	GKGCMC	1 cut(s)	672
BstSNI	TACGTA	1 cut(s)	82
BstUI	CGCG	1 cut(s)	380
BstV1I	GCAGC	2 cut(s)	530, 676
BstV2I	GAAGAC	1 cut(s)	220
BstX2I	RGATCY	1 cut(s)	162
BstYI	RGATCY	1 cut(s)	162
BtgZI	GCGATG	1 cut(s)	220
BtsI	GCAGTG	1 cut(s)	832
BtsIMutI	CAGTG	4 cut(s)	342, 756, 757, 832
Cfr13I	GGNCC	2 cut(s)	435, 569
Csp6I	GTAC	3 cut(s)	10, 388, 643
CviAII	CATG	2 cut(s)	47, 875
CviQI	GTAC	3 cut(s)	10, 388, 643
DdeI	CTNAG	4 cut(s)	103, 578, 700, 856
DpnI	GATC	6 cut(s)	164, 177, 288, 441, 515, 770
DpnII	GATC	6 cut(s)	162, 175, 286, 439, 513, 768
DrdI	GACNNNNNNGTC	1 cut(s)	206
DseDI	GACNNNNNNGTC	1 cut(s)	206
Eco105I	TACGTA	1 cut(s)	82
Eco31I	GGTCTC	1 cut(s)	806
Eco47I	GGWCC	2 cut(s)	435, 569
Eco57I	CTGAAG	1 cut(s)	637
Eco88I	CYCGRG	1 cut(s)	243
Eco91I	GGTNACC	1 cut(s)	16
EcoO65I	GGTNACC	1 cut(s)	16
FaeI	CATG	2 cut(s)	50, 878
FaqI	GGGAC	2 cut(s)	146, 643
FatI	CATG	2 cut(s)	46, 874
Fnu4HI	GCNGC	2 cut(s)	519, 690
Fsp4HI	GCNGC	2 cut(s)	519, 690
FspBI	CTAG	2 cut(s)	65, 539
GluI	GCNGC	2 cut(s)	519, 690
HapII	CCGG	1 cut(s)	21
Hin1II	CATG	2 cut(s)	50, 878
HincII	GTYRAC	1 cut(s)	211
HindII	GTYRAC	1 cut(s)	211
HindIII	AAGCTT	2 cut(s)	193, 504
HinfI	GANTC	6 cut(s)	101, 225, 425, 631, 732, 786
HpaII	CCGG	1 cut(s)	21
Hpy166II	GTNNAC	2 cut(s)	211, 670
Hpy188I	TCNGA	3 cut(s)	106, 766, 959
Hpy188III	TCNNGA	8 cut(s)	65, 243, 260, 275, 336, 443, 726, 941
Hpy8I	GTNNAC	2 cut(s)	211, 670
HpyAV	CCTTC	2 cut(s)	827, 904
HpyCH4III	ACNGT	6 cut(s)	94, 215, 346, 387, 392, 926
HpyCH4IV	ACGT	2 cut(s)	81, 641
HpyCH4V	TGCA	4 cut(s)	329, 453, 670, 692
HpyF3I	CTNAG	4 cut(s)	103, 578, 700, 856
HpySE526I	ACGT	2 cut(s)	81, 641
Hsp92II	CATG	2 cut(s)	50, 878
KpnI	GGTACC	1 cut(s)	391
Kzo9I	GATC	6 cut(s)	162, 175, 286, 439, 513, 768
LmnI	GCTCC	2 cut(s)	143, 602
LpnPI	CCDG	7 cut(s)	34, 43, 245, 260, 640, 711, 745
Lsp1109I	GCAGC	2 cut(s)	530, 676
LweI	GCATC	4 cut(s)	248, 388, 731, 768
MaeI	CTAG	2 cut(s)	65, 539
MaeII	ACGT	2 cut(s)	81, 641
MaeIII	GTNAC	2 cut(s)	16, 57
MalI	GATC	6 cut(s)	164, 177, 288, 441, 515, 770
MboI	GATC	6 cut(s)	162, 175, 286, 439, 513, 768
MboII	GAAGA	9 cut(s)	122, 157, 172, 220, 360, 363, 722, 741, 842
MflI	RGATCY	1 cut(s)	162
MhlI	GDGCHC	1 cut(s)	672
MluCI	AATT	3 cut(s)	68, 281, 465
MlyI	GAGTC	3 cut(s)	95, 234, 625
MnlI	CCTC	8 cut(s)	17, 52, 181, 504, 612, 653, 858, 901
MroXI	GAANNNNTTC	1 cut(s)	164
MseI	TTAA	3 cut(s)	137, 420, 905
MslI	CAYNNNNRTG	1 cut(s)	776
MspA1I	CMGCKG	1 cut(s)	521
MspI	CCGG	1 cut(s)	21
MvnI	CGCG	1 cut(s)	380
NdeII	GATC	6 cut(s)	162, 175, 286, 439, 513, 768
NlaIII	CATG	2 cut(s)	50, 878
NlaIV	GGNNCC	2 cut(s)	28, 389
NmuCI	GTSAC	1 cut(s)	57
NspV	TTCGAA	1 cut(s)	168
PaeR7I	CTCGAG	1 cut(s)	243
PdmI	GAANNNNTTC	1 cut(s)	164
PfeI	GAWTC	3 cut(s)	425, 732, 786
PkrI	GCNGC	2 cut(s)	520, 691
PleI	GAGTC	3 cut(s)	95, 233, 625
PpsI	GAGTC	3 cut(s)	95, 233, 625
Ppu21I	YACGTR	1 cut(s)	82
PsiI	TTATAA	1 cut(s)	480
PspEI	GGTNACC	1 cut(s)	16
PspN4I	GGNNCC	2 cut(s)	28, 389
PspPI	GGNCC	2 cut(s)	435, 569
PsuI	RGATCY	1 cut(s)	162
PvuII	CAGCTG	1 cut(s)	521
RsaI	GTAC	3 cut(s)	11, 389, 644
RsaNI	GTAC	3 cut(s)	10, 388, 643
RseI	CAYNNNNRTG	1 cut(s)	776
SaqAI	TTAA	3 cut(s)	137, 420, 905
SatI	GCNGC	2 cut(s)	519, 690
Sau3AI	GATC	6 cut(s)	162, 175, 286, 439, 513, 768
Sau96I	GGNCC	2 cut(s)	435, 569
ScaI	AGTACT	1 cut(s)	11
SchI	GAGTC	3 cut(s)	95, 234, 625
SduI	GDGCHC	1 cut(s)	672
SfaNI	GCATC	4 cut(s)	248, 388, 731, 768
Sfr274I	CTCGAG	1 cut(s)	243
SfuI	TTCGAA	1 cut(s)	168
SinI	GGWCC	2 cut(s)	435, 569
SlaI	CTCGAG	1 cut(s)	243
SmiMI	CAYNNNNRTG	1 cut(s)	776
SmlI	CTYRAG	1 cut(s)	243
SmoI	CTYRAG	1 cut(s)	243
SnaBI	TACGTA	1 cut(s)	82
Sse9I	AATT	3 cut(s)	68, 281, 465
SsiI	CCGC	1 cut(s)	378
SspMI	CTAG	2 cut(s)	65, 539
TaaI	ACNGT	6 cut(s)	94, 215, 346, 387, 392, 926
TaiI	ACGT	2 cut(s)	84, 644
TaqI	TCGA	3 cut(s)	168, 244, 743
TaqII	GACCGA	1 cut(s)	452
TasI	AATT	3 cut(s)	68, 281, 465
TatI	WGTACW	1 cut(s)	9
TfiI	GAWTC	3 cut(s)	425, 732, 786
Tru1I	TTAA	3 cut(s)	137, 420, 905
Tru9I	TTAA	3 cut(s)	137, 420, 905
TscAI	CASTG	4 cut(s)	349, 756, 764, 832
TseFI	GTSAC	1 cut(s)	57
TseI	GCWGC	2 cut(s)	518, 689
Tsp45I	GTSAC	1 cut(s)	57
TspDTI	ATGAA	5 cut(s)	123, 323, 332, 438, 891
TspRI	CASTG	4 cut(s)	349, 756, 764, 832
VneI	GTGCAC	1 cut(s)	668
VpaK11BI	GGWCC	2 cut(s)	435, 569
XapI	RAATTY	3 cut(s)	68, 281, 465
XbaI	TCTAGA	1 cut(s)	64
XhoI	CTCGAG	1 cut(s)	243
XmnI	GAANNNNTTC	1 cut(s)	164
XspI	CTAG	2 cut(s)	65, 539
ZrmI	AGTACT	1 cut(s)	11

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.