pycom13g03700

Tetraketide alpha-pyrone reductase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0008036
Physical Location & Seq
Forward (+)
2464540 .. 2466855
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03700.1

Sequence Viewer

Length: 615 bp
ATGAGTTATCCACCAACTTTAAAGATAAACCCACACAAATATCCACAAAACCAGACAGAGAAGATGCCAGAATACTGTGTAACTGGAGGAACAGGCTTCCTAGCTGCTTACTTAGTGAAGGCCTTGCTAGACAAGGGCCATGTTGTCCGCACCACGGTTCGAAACCCAGAGGATGTGGGAAAGGTGGGTTATTTGTGGGAGTTGAATGGAGCCAGGGAAAGGCTAAAGATGTTGAAAGCTGATTTGATGGTGGAAGGAAGCTTTGATGAGGCTGTACGAGGCGTCGATGGAGTCTTCCACACCGCATCACCCGTTCTTGTTCCATACGACGACAATGTTCAGGGAAATTTGATTGATCCATGTATAAGGGGCACACTGAATGTTCTGAATTCATGCTCAAAAGCACGTGTGAAACGGGTTGTGCTCACCTCTTCTTGCTCTTCAATAAGATATCGTTACGATGCCCAGCAGGCCTCTCCTCTCAACGAATCACATTGGAGTGATGCTGAGTACTGCAAACACTACAATCTCTGGTATGCTTATGCAAAAACTACAGCAGAGAAGGAGGCCTGGAGAATTGCTGGGGAAAGCGGAATGGATCGATCGTTGTCGTGA

Protein Analysis

205

Amino Acids

22.87

Weight (kDa)

8.21

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 26 - 199 6.6e-18 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 27 - 188 1.9e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 27 - 169 1.4e-10 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 27 - 191 6.8e-10 Male sterility protein
NmrA PF05368 27 - 108 9.1e-08 NmrA-like family
Polysacc_synt_2 PF02719 27 - 145 7.6e-06 Polysaccharide biosynthesis protein
NAD_binding_10 PF13460 29 - 163 7e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 148, 303, 591
AclWI GGATC 2 cut(s) 350, 606
AcsI RAATTY 2 cut(s) 346, 388
AcvI CACGTG 1 cut(s) 407
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 2 cut(s) 276, 512
AfiI CCNNNNNNNGG 2 cut(s) 154, 219
AflIII ACRYGT 1 cut(s) 406
AgsI TTSAA 3 cut(s) 205, 235, 444
AjnI CCWGG 2 cut(s) 212, 569
AluBI AGCT 3 cut(s) 104, 239, 261
AluI AGCT 3 cut(s) 104, 239, 261
Alw21I GWGCWC 1 cut(s) 426
AlwI GGATC 2 cut(s) 350, 606
AoxI GGCC 4 cut(s) 120, 136, 471, 567
ApeKI GCWGC 1 cut(s) 104
ApoI RAATTY 2 cut(s) 346, 388
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 300, 418
AsuII TTCGAA 1 cut(s) 160
BaeGI GKGCMC 1 cut(s) 374
BbrPI CACGTG 1 cut(s) 407
BbsI GAAGAC 1 cut(s) 286
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 1 cut(s) 91
BccI CCATC 2 cut(s) 241, 281
BciT130I CCWGG 2 cut(s) 214, 571
BfaI CTAG 2 cut(s) 101, 128
BfmI CTRYAG 1 cut(s) 552
BglI GCCNNNNNGGC 1 cut(s) 470
BisI GCNGC 1 cut(s) 105
BlsI GCNGC 1 cut(s) 106
BmcAI AGTACT 1 cut(s) 512
Bme1390I CCNGG 2 cut(s) 214, 571
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 2 cut(s) 214, 571
BmsI GCATC 4 cut(s) 54, 314, 451, 493
BpiI GAAGAC 1 cut(s) 286
BpmI CTGGAG 2 cut(s) 105, 592
Bpu14I TTCGAA 1 cut(s) 160
Bsa29I ATCGAT 1 cut(s) 601
BsaAI YACGTR 1 cut(s) 407
BsaHI GRCGYC 1 cut(s) 282
BsaJI CCNNGG 2 cut(s) 153, 213
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 219
Bse1I ACTGG 1 cut(s) 88
BseBI CCWGG 2 cut(s) 214, 571
BseCI ATCGAT 1 cut(s) 601
BseDI CCNNGG 2 cut(s) 153, 213
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 2 cut(s) 154, 219
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 1 cut(s) 468
BseSI GKGCMC 1 cut(s) 374
BseXI GCAGC 1 cut(s) 91
BseYI CCCAGC 2 cut(s) 465, 581
Bsh1285I CGRYCG 1 cut(s) 605
BshFI GGCC 4 cut(s) 122, 138, 473, 569
BshVI ATCGAT 1 cut(s) 601
BsiEI CGRYCG 1 cut(s) 605
BsiHKAI GWGCWC 1 cut(s) 426
BslI CCNNNNNNNGG 2 cut(s) 154, 219
BsnI GGCC 4 cut(s) 122, 138, 473, 569
Bsp119I TTCGAA 1 cut(s) 160
Bsp1286I GDGCHC 2 cut(s) 374, 426
Bsp143I GATC 3 cut(s) 355, 598, 602
BspACI CCGC 3 cut(s) 148, 303, 591
BspANI GGCC 4 cut(s) 122, 138, 473, 569
BspCNI CTCAG 1 cut(s) 499
BspDI ATCGAT 1 cut(s) 601
BspLI GGNNCC 1 cut(s) 211
BspPI GGATC 2 cut(s) 350, 606
BspQI GCTCTTC 1 cut(s) 445
BspT104I TTCGAA 1 cut(s) 160
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 2 cut(s) 153, 213
BssMI GATC 3 cut(s) 355, 598, 602
BssNI GRCGYC 1 cut(s) 282
Bst2UI CCWGG 2 cut(s) 214, 571
Bst4CI ACNGT 2 cut(s) 77, 157
Bst6I CTCTTC 2 cut(s) 436, 445
BstACI GRCGYC 1 cut(s) 282
BstBAI YACGTR 1 cut(s) 407
BstBI TTCGAA 1 cut(s) 160
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 2 cut(s) 112, 507
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 178
BstKTI GATC 3 cut(s) 358, 601, 605
BstMBI GATC 3 cut(s) 355, 598, 602
BstMCI CGRYCG 1 cut(s) 605
BstMWI GCNNNNNNNGC 1 cut(s) 470
BstNI CCWGG 2 cut(s) 214, 571
BstSCI CCNGG 2 cut(s) 212, 569
BstSFI CTRYAG 1 cut(s) 552
BstSLI GKGCMC 1 cut(s) 374
BstV1I GCAGC 1 cut(s) 91
BstV2I GAAGAC 1 cut(s) 286
Bsu15I ATCGAT 1 cut(s) 601
BsuRI GGCC 4 cut(s) 122, 138, 473, 569
BsuTUI ATCGAT 1 cut(s) 601
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 178
BtsIMutI CAGTG 1 cut(s) 374
Cac8I GCNNGC 1 cut(s) 471
Cfr13I GGNCC 1 cut(s) 136
ClaI ATCGAT 1 cut(s) 601
CseI GACGC 1 cut(s) 271
Csp6I GTAC 2 cut(s) 275, 511
CviAII CATG 3 cut(s) 140, 360, 393
CviQI GTAC 2 cut(s) 275, 511
DdeI CTNAG 2 cut(s) 112, 507
DpnI GATC 3 cut(s) 357, 600, 604
DpnII GATC 3 cut(s) 355, 598, 602
DraI TTTAAA 1 cut(s) 21
Eam1104I CTCTTC 2 cut(s) 436, 445
EarI CTCTTC 2 cut(s) 436, 445
Eco147I AGGCCT 3 cut(s) 122, 473, 569
Eco32I GATATC 1 cut(s) 452
Eco72I CACGTG 1 cut(s) 407
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 2 cut(s) 212, 569
EcoRV GATATC 1 cut(s) 452
FaeI CATG 3 cut(s) 143, 363, 396
FaiI YATR 7 cut(s) 141, 325, 361, 365, 394, 537, 543
FatI CATG 3 cut(s) 139, 359, 392
Fnu4HI GCNGC 1 cut(s) 105
FokI GGATG 1 cut(s) 185
Fsp4HI GCNGC 1 cut(s) 105
FspBI CTAG 2 cut(s) 101, 128
GluI GCNGC 1 cut(s) 105
GsaI CCCAGC 2 cut(s) 469, 585
GsuI CTGGAG 2 cut(s) 105, 592
HaeIII GGCC 4 cut(s) 122, 138, 473, 569
HgaI GACGC 1 cut(s) 271
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 3 cut(s) 143, 363, 396
HindIII AAGCTT 1 cut(s) 259
HinfI GANTC 2 cut(s) 291, 488
HphI GGTGA 2 cut(s) 300, 418
Hpy188I TCNGA 1 cut(s) 387
Hpy188III TCNNGA 1 cut(s) 612
Hpy99I CGWCG 2 cut(s) 287, 332
HpyAV CCTTC 3 cut(s) 112, 248, 556
HpyCH4III ACNGT 2 cut(s) 77, 157
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 2 cut(s) 516, 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 470
HpyF3I CTNAG 2 cut(s) 112, 507
HpySE526I ACGT 1 cut(s) 406
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 3 cut(s) 143, 363, 396
Kzo9I GATC 3 cut(s) 355, 598, 602
LguI GCTCTTC 1 cut(s) 445
LmnI GCTCC 1 cut(s) 209
Lsp1109I GCAGC 1 cut(s) 91
LweI GCATC 4 cut(s) 54, 314, 451, 493
MaeI CTAG 2 cut(s) 101, 128
MaeII ACGT 1 cut(s) 406
MaeIII GTNAC 2 cut(s) 79, 455
MalI GATC 3 cut(s) 357, 600, 604
MboI GATC 3 cut(s) 355, 598, 602
MboII GAAGA 4 cut(s) 73, 286, 423, 432
MhlI GDGCHC 2 cut(s) 374, 426
MluCI AATT 3 cut(s) 346, 388, 576
MlyI GAGTC 1 cut(s) 300
MnlI CCTC 8 cut(s) 80, 163, 262, 272, 439, 484, 489, 559
MseI TTAA 1 cut(s) 20
MslI CAYNNNNRTG 1 cut(s) 498
MspR9I CCNGG 2 cut(s) 214, 571
MvaI CCWGG 2 cut(s) 214, 571
MwoI GCNNNNNNNGC 1 cut(s) 470
NdeII GATC 3 cut(s) 355, 598, 602
NlaIII CATG 3 cut(s) 143, 363, 396
NlaIV GGNNCC 1 cut(s) 211
NspV TTCGAA 1 cut(s) 160
PceI AGGCCT 3 cut(s) 122, 473, 569
PciSI GCTCTTC 1 cut(s) 445
PfeI GAWTC 1 cut(s) 488
PkrI GCNGC 1 cut(s) 106
Ple19I CGATCG 1 cut(s) 605
PleI GAGTC 1 cut(s) 299
PmaCI CACGTG 1 cut(s) 407
PmlI CACGTG 1 cut(s) 407
PpsI GAGTC 1 cut(s) 299
Ppu21I YACGTR 1 cut(s) 407
Psp6I CCWGG 2 cut(s) 212, 569
PspCI CACGTG 1 cut(s) 407
PspFI CCCAGC 2 cut(s) 465, 581
PspGI CCWGG 2 cut(s) 212, 569
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 136
PvuI CGATCG 1 cut(s) 605
RsaI GTAC 2 cut(s) 276, 512
RsaNI GTAC 2 cut(s) 275, 511
RseI CAYNNNNRTG 1 cut(s) 498
SapI GCTCTTC 1 cut(s) 445
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 1 cut(s) 105
Sau3AI GATC 3 cut(s) 355, 598, 602
Sau96I GGNCC 1 cut(s) 136
ScaI AGTACT 1 cut(s) 512
SchI GAGTC 1 cut(s) 300
ScrFI CCNGG 2 cut(s) 214, 571
SduI GDGCHC 2 cut(s) 374, 426
SetI ASST 6 cut(s) 106, 186, 241, 263, 409, 431
SfaNI GCATC 4 cut(s) 54, 314, 451, 493
SfcI CTRYAG 1 cut(s) 552
SfuI TTCGAA 1 cut(s) 160
SmiMI CAYNNNNRTG 1 cut(s) 498
Sse9I AATT 3 cut(s) 346, 388, 576
SseBI AGGCCT 3 cut(s) 122, 473, 569
SsiI CCGC 3 cut(s) 148, 303, 591
SspMI CTAG 2 cut(s) 101, 128
StuI AGGCCT 3 cut(s) 122, 473, 569
StyD4I CCNGG 2 cut(s) 212, 569
TaaI ACNGT 2 cut(s) 77, 157
TaiI ACGT 1 cut(s) 409
TaqI TCGA 3 cut(s) 160, 285, 601
TasI AATT 3 cut(s) 346, 388, 576
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 1 cut(s) 488
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 1 cut(s) 381
TseI GCWGC 1 cut(s) 104
TspDTI ATGAA 1 cut(s) 381
TspRI CASTG 1 cut(s) 381
XapI RAATTY 2 cut(s) 346, 388
XspI CTAG 2 cut(s) 101, 128
ZrmI AGTACT 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.