RLG00000006604

Tetraketide alpha-pyrone reductase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008036
Physical Location & Seq
Reverse (-)
8904644 .. 8906723
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006604

Sequence Viewer

Length: 954 bp
ATGCCTGAATATTGTGTGACTGGAGGTACAGGGTTCATAGCTGCCTACTTGGTGAAGGCTTTGCTAGAAAATGGCCATGTTGTGCGGGCCACTGTGCGAGACCCAGAGGATTTGGGGAAGGTTGGTTATCTGCGCGAGTTGAATGGAGCCAAGGAAAGGCTTAAGCTGATCAAAGCTGATCTAATGGTGGAAGGAAGCTTTGATGAAGCCATACAAGGAGTGGATGGGGTTTTCCACACCGCATCACCAGTGCTCGTTCCGTACGATAACAATGCGACTTTGATTGATCCATGTATAAAGGGCACCTTGAATGTGCTGAGTTCCTGTTCGAAGGCACGCGTGAAAAGGGTTGTGCTCACATCTTCTTGTTCTTCAATAAGATACCGTTACGATGCCCAACAGGCCTCTCCTCTCAATGACTCACATTGGAGCGATCCTGAATACTGCAAACGCTTCAATCTTTGGTACGCCTATGCAAAGACAACAGCGGAAAAAGAGGCGTGGAGAATCGCAGAGGAGAGTGGAATGGATCTAGTGGTGGTGAATCCGTCCTTCGTGGTTGGTCCTCTGCTAGCACCACAACCTTCAAGCACAATACTAATGATACTGGCAATACTTAAAGGTTTAAGAGGGGAATACCCAAATACAACAGTAGGATTTGTGCACATAGATGATGTGGTAGCTACACACATTCTGGCAATGGAGGAAAGAAAAGCATCAGGCAGGCTTATATGTTCAAGTTCGGTAGCGCACTGGTCGCAGATCATTGAGATGCTCAAGGCCAAATATCCATCTTATTCATATGAAAGCAAGTGTAGCAGCAAGGAAGGAGACAATAACCCACATAGCATGGACACCACTAAAATTGCTCAACTGGGTTTTCCTCCTTTCAAAACGCTCGAAAAAATGTTTGATGACTGCATCACAAGTTTTCAAGAGAAAGGGTTTCTGTGA

Protein Analysis

318

Amino Acids

35.06

Weight (kDa)

6.19

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 5 - 239 2.3e-28 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 5 - 122 3.9e-06 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 6 - 235 6.1e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 6 - 263 2.6e-16 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 6 - 196 1.8e-14 Male sterility protein
NmrA PF05368 6 - 90 1.3e-09 NmrA-like family
NAD_binding_10 PF13460 8 - 185 6.6e-16 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 302
AccII CGCG 2 cut(s) 135, 339
AciI CCGC 3 cut(s) 85, 240, 488
AclWI GGATC 3 cut(s) 281, 428, 537
AcoI YGGCCR 1 cut(s) 73
AfaI GTAC 3 cut(s) 28, 263, 467
AfiI CCNNNNNNNGG 1 cut(s) 156
AflII CTTAAG 1 cut(s) 161
AflIII ACRYGT 1 cut(s) 337
AgsI TTSAA 8 cut(s) 142, 310, 375, 457, 588, 738, 892, 935
AluBI AGCT 5 cut(s) 41, 166, 176, 198, 683
AluI AGCT 5 cut(s) 41, 166, 176, 198, 683
Alw21I GWGCWC 3 cut(s) 255, 357, 666
Alw26I GTCTC 2 cut(s) 93, 825
Alw44I GTGCAC 1 cut(s) 662
AlwI GGATC 3 cut(s) 281, 428, 537
AoxI GGCC 4 cut(s) 73, 87, 402, 780
ApaLI GTGCAC 1 cut(s) 662
ApeKI GCWGC 2 cut(s) 41, 819
AspLEI GCGC 2 cut(s) 135, 751
AspS9I GGNCC 2 cut(s) 87, 563
AsuHPI GGTGA 3 cut(s) 64, 237, 553
AsuII TTCGAA 1 cut(s) 329
AsuNHI GCTAGC 1 cut(s) 571
AvaII GGWCC 1 cut(s) 563
BaeGI GKGCMC 2 cut(s) 305, 666
BalI TGGCCA 1 cut(s) 75
BanI GGYRCC 1 cut(s) 302
Bbv12I GWGCWC 3 cut(s) 255, 357, 666
BbvI GCAGC 2 cut(s) 28, 831
BccI CCATC 2 cut(s) 218, 799
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BclI TGATCA 1 cut(s) 168
BcoDI GTCTC 2 cut(s) 93, 825
BfaI CTAG 3 cut(s) 65, 533, 572
BfrI CTTAAG 1 cut(s) 161
BglI GCCNNNNNGGC 1 cut(s) 401
BisI GCNGC 2 cut(s) 42, 820
BlsI GCNGC 2 cut(s) 43, 821
Bme18I GGWCC 1 cut(s) 563
BmgT120I GGNCC 2 cut(s) 87, 563
BmiI GGNNCC 2 cut(s) 148, 304
BmrI ACTGGG 1 cut(s) 884
BmsI GCATC 5 cut(s) 251, 382, 725, 762, 930
BmtI GCTAGC 1 cut(s) 575
BmuI ACTGGG 1 cut(s) 884
BpmI CTGGAG 1 cut(s) 42
Bpu14I TTCGAA 1 cut(s) 329
BpuEI CTTGAG 1 cut(s) 761
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 150
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 5 cut(s) 25, 248, 612, 758, 879
Bse3DI GCAATG 1 cut(s) 705
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 5 cut(s) 25, 248, 612, 758, 879
BseRI GAGGAG 2 cut(s) 399, 530
BseSI GKGCMC 2 cut(s) 305, 666
BseXI GCAGC 2 cut(s) 28, 831
Bsh1236I CGCG 2 cut(s) 135, 339
BshFI GGCC 4 cut(s) 75, 89, 404, 782
BshNI GGYRCC 1 cut(s) 302
BsiHKAI GWGCWC 3 cut(s) 255, 357, 666
BsiWI CGTACG 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 2 cut(s) 93, 825
BsnI GGCC 4 cut(s) 75, 89, 404, 782
Bso31I GGTCTC 1 cut(s) 93
Bsp119I TTCGAA 1 cut(s) 329
Bsp1286I GDGCHC 4 cut(s) 255, 305, 357, 666
Bsp143I GATC 6 cut(s) 168, 178, 286, 433, 529, 762
BspACI CCGC 3 cut(s) 85, 240, 488
BspANI GGCC 4 cut(s) 75, 89, 404, 782
BspCNI CTCAG 1 cut(s) 309
BspFNI CGCG 2 cut(s) 135, 339
BspLI GGNNCC 2 cut(s) 148, 304
BspOI GCTAGC 1 cut(s) 575
BspPI GGATC 3 cut(s) 281, 428, 537
BspT104I TTCGAA 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 302
BspTI CTTAAG 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 93
BsrDI GCAATG 1 cut(s) 705
BsrI ACTGG 5 cut(s) 25, 248, 612, 758, 879
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 6 cut(s) 168, 178, 286, 433, 529, 762
BssT1I CCWWGG 1 cut(s) 150
Bst4CI ACNGT 3 cut(s) 94, 386, 652
BstAFI CTTAAG 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 329
BstC8I GCNNGC 4 cut(s) 87, 337, 573, 725
BstDEI CTNAG 1 cut(s) 317
BstF5I GGATG 1 cut(s) 229
BstFNI CGCG 2 cut(s) 135, 339
BstHHI GCGC 2 cut(s) 135, 751
BstKTI GATC 6 cut(s) 171, 181, 289, 436, 532, 765
BstMAI GTCTC 2 cut(s) 93, 825
BstMBI GATC 6 cut(s) 168, 178, 286, 433, 529, 762
BstMWI GCNNNNNNNGC 3 cut(s) 401, 757, 816
BstSLI GKGCMC 2 cut(s) 305, 666
BstUI CGCG 2 cut(s) 135, 339
BstV1I GCAGC 2 cut(s) 28, 831
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
BsuRI GGCC 4 cut(s) 75, 89, 404, 782
BtsCI GGATG 1 cut(s) 229
BtsIMutI CAGTG 3 cut(s) 90, 255, 751
Cac8I GCNNGC 4 cut(s) 87, 337, 573, 725
CfoI GCGC 2 cut(s) 135, 751
Cfr13I GGNCC 2 cut(s) 87, 563
Csp6I GTAC 3 cut(s) 27, 262, 466
CspCI CAANNNNNGTGG 2 cut(s) 847, 882
CviAII CATG 3 cut(s) 77, 291, 850
CviQI GTAC 3 cut(s) 27, 262, 466
DdeI CTNAG 1 cut(s) 317
DpnI GATC 6 cut(s) 170, 180, 288, 435, 531, 764
DpnII GATC 6 cut(s) 168, 178, 286, 433, 529, 762
EaeI YGGCCR 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 404
Eco31I GGTCTC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 563
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 3 cut(s) 80, 294, 853
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FatI CATG 3 cut(s) 76, 290, 849
FauI CCCGC 1 cut(s) 78
FauNDI CATATG 1 cut(s) 802
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 2 cut(s) 42, 820
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 2 cut(s) 42, 820
FspBI CTAG 3 cut(s) 65, 533, 572
GlaI GCGC 2 cut(s) 134, 750
GluI GCNGC 2 cut(s) 42, 820
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 4 cut(s) 75, 89, 404, 782
HhaI GCGC 2 cut(s) 135, 751
Hin1II CATG 3 cut(s) 80, 294, 853
Hin6I GCGC 2 cut(s) 133, 749
HinP1I GCGC 2 cut(s) 133, 749
HindIII AAGCTT 1 cut(s) 196
HinfI GANTC 3 cut(s) 419, 507, 544
HphI GGTGA 3 cut(s) 64, 237, 553
Hpy166II GTNNAC 1 cut(s) 664
Hpy188III TCNNGA 2 cut(s) 437, 935
Hpy8I GTNNAC 1 cut(s) 664
HpyAV CCTTC 7 cut(s) 49, 112, 185, 325, 562, 594, 821
HpyCH4III ACNGT 3 cut(s) 94, 386, 652
HpyCH4V TGCA 4 cut(s) 447, 476, 664, 921
HpyF10VI GCNNNNNNNGC 3 cut(s) 401, 757, 816
HpyF3I CTNAG 1 cut(s) 317
Hsp92II CATG 3 cut(s) 80, 294, 853
HspAI GCGC 2 cut(s) 133, 749
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 6 cut(s) 168, 178, 286, 433, 529, 762
LmnI GCTCC 2 cut(s) 146, 429
Lsp1109I GCAGC 2 cut(s) 28, 831
LweI GCATC 5 cut(s) 251, 382, 725, 762, 930
MaeI CTAG 3 cut(s) 65, 533, 572
MaeIII GTNAC 2 cut(s) 16, 386
MalI GATC 6 cut(s) 170, 180, 288, 435, 531, 764
MboI GATC 6 cut(s) 168, 178, 286, 433, 529, 762
MboII GAAGA 2 cut(s) 354, 363
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 4 cut(s) 255, 305, 357, 666
MlsI TGGCCA 1 cut(s) 75
MluCI AATT 1 cut(s) 864
MluI ACGCGT 1 cut(s) 337
MluNI TGGCCA 1 cut(s) 75
MlyI GAGTC 1 cut(s) 413
Mox20I TGGCCA 1 cut(s) 75
MscI TGGCCA 1 cut(s) 75
MseI TTAA 3 cut(s) 162, 618, 626
MslI CAYNNNNRTG 2 cut(s) 669, 770
Msp20I TGGCCA 1 cut(s) 75
MspA1I CMGCKG 1 cut(s) 488
MspCI CTTAAG 1 cut(s) 161
MvnI CGCG 2 cut(s) 135, 339
MwoI GCNNNNNNNGC 3 cut(s) 401, 757, 816
NdeI CATATG 1 cut(s) 802
NdeII GATC 6 cut(s) 168, 178, 286, 433, 529, 762
NheI GCTAGC 1 cut(s) 571
NlaIII CATG 3 cut(s) 80, 294, 853
NlaIV GGNNCC 2 cut(s) 148, 304
NmuCI GTSAC 1 cut(s) 16
NspV TTCGAA 1 cut(s) 329
PceI AGGCCT 1 cut(s) 404
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PfeI GAWTC 2 cut(s) 507, 544
Pfl23II CGTACG 1 cut(s) 261
PkrI GCNGC 2 cut(s) 43, 821
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
PspLI CGTACG 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 148, 304
PspPI GGNCC 2 cut(s) 87, 563
PsuI RGATCY 1 cut(s) 529
RsaI GTAC 3 cut(s) 28, 263, 467
RsaNI GTAC 3 cut(s) 27, 262, 466
RseI CAYNNNNRTG 2 cut(s) 669, 770
SaqAI TTAA 3 cut(s) 162, 618, 626
SatI GCNGC 2 cut(s) 42, 820
Sau3AI GATC 6 cut(s) 168, 178, 286, 433, 529, 762
Sau96I GGNCC 2 cut(s) 87, 563
SchI GAGTC 1 cut(s) 413
SduI GDGCHC 4 cut(s) 255, 305, 357, 666
SfaNI GCATC 5 cut(s) 251, 382, 725, 762, 930
SfuI TTCGAA 1 cut(s) 329
SinI GGWCC 1 cut(s) 563
SmiMI CAYNNNNRTG 2 cut(s) 669, 770
SmlI CTYRAG 2 cut(s) 161, 776
SmoI CTYRAG 2 cut(s) 161, 776
Sse9I AATT 1 cut(s) 864
SseBI AGGCCT 1 cut(s) 404
SsiI CCGC 3 cut(s) 85, 240, 488
SspI AATATT 1 cut(s) 11
SspMI CTAG 3 cut(s) 65, 533, 572
StuI AGGCCT 1 cut(s) 404
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 3 cut(s) 94, 386, 652
TaqI TCGA 2 cut(s) 329, 900
TasI AATT 1 cut(s) 864
TfiI GAWTC 2 cut(s) 507, 544
Tru1I TTAA 3 cut(s) 162, 618, 626
Tru9I TTAA 3 cut(s) 162, 618, 626
TscAI CASTG 3 cut(s) 97, 255, 758
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 2 cut(s) 41, 819
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 4 cut(s) 25, 219, 789, 819
TspGWI ACGGA 2 cut(s) 249, 537
TspRI CASTG 3 cut(s) 97, 255, 758
Vha464I CTTAAG 1 cut(s) 161
VneI GTGCAC 1 cut(s) 662
VpaK11BI GGWCC 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XspI CTAG 3 cut(s) 65, 533, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.