MD13G1042500.v1.1

Tetraketide alpha-pyrone reductase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008036
Physical Location & Seq
Forward (+)
2955764 .. 2957804
2041 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1042500.v1.1.491

Sequence Viewer

Length: 960 bp
ATGCCTGAATACTGTGTAACTGGAGGAACAGGCTTCATAGCTGCTTACTTAGTGAAAGCCTTGCTAGACAAGGGCCATGTTGTCCGCACCACGGTTCGAAACCCAGAGGATGGGGGGAAGGTGGGTTATTTGTGGGAGTTTAATGGAGCCAAGGAAAGGCTCAAGGTGTTGAAAGCTGATTTGATGGTGGAAGGAAGCTTTGATGAGGCTGTACGAGGCGTCGATGGAGTCTTCCACACTGCATCACCCGTTCTTGTTCCATACGACGACAATGTTCAGGCAAATTTGATTGATCCATGTATAAAGGGCACACTGAATGTTCTGAATTCATGCTCAAAAGCACGTGTGAAACGGGTTGTGCTCACCTCTTCTTGCTCTTCAATAAGATATCGTTACGATGCCCAGCAGGCCTCTCCTCTCAACGAATCACATTGGAGTGATGCTGAGTACTGCAAACACTACAATCTCTGGTATGCTTATGCAAAAACTACAGCAGAGAAGGAGGCCTGGAGAATTGCTGGGGAGAGCGGAATGGATCTCGTTGTGGTGAATCCGTCGTTCGTGGTTGGTCCTCTGCTAGCACCACAACCAACCAGCACTCTATATATGATACTGGCTATACTGAAAGGTTCAAGAGGGGAGTACCCGAACACGACAGTAGGATTTGTGCACATAGATGATGTGGTAGCTGCGCACATTTTAGCAATGGAGGAAAGCAAAGCGTCGGGAAGGCTGATATGTTCGAGCTCGGTTGCGCACTGGTCGGAGATCATTAAGATGCTGAGGGCCAAATATCCATCCTATCCATATGAAAACAAGTGTAGCAGCGTGGAAGGAGACAGTAATCCACATAGCATGGATACCAGTAAAATTGCTCAGCTGGGATTTCCACCTTTCAAAACGCTTGAAACAATGTTCGATGACTGCATCACTAGTTTCCAACAGAAAGGATTTCTATGA

Protein Analysis

320

Amino Acids

35.19

Weight (kDa)

6.25

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 5 - 241 1.4e-27 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 6 - 237 5e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 6 - 260 1.9e-14 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 6 - 197 2.5e-13 Male sterility protein
NmrA PF05368 6 - 87 6.8e-08 NmrA-like family
Polysacc_synt_2 PF02719 6 - 124 5.7e-06 Polysaccharide biosynthesis protein
NAD_binding_10 PF13460 8 - 187 5.8e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 693, 756
AccB7I CCANNNNNTGG 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 528
AciI CCGC 2 cut(s) 85, 528
AclWI GGATC 2 cut(s) 287, 543
AcsI RAATTY 2 cut(s) 283, 325
AcvI CACGTG 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 3 cut(s) 213, 449, 644
AfiI CCNNNNNNNGG 3 cut(s) 91, 110, 156
AflIII ACRYGT 1 cut(s) 343
AgsI TTSAA 5 cut(s) 172, 381, 633, 898, 908
AhlI ACTAGT 1 cut(s) 932
AjnI CCWGG 1 cut(s) 506
AluBI AGCT 6 cut(s) 41, 176, 198, 689, 747, 880
AluI AGCT 6 cut(s) 41, 176, 198, 689, 747, 880
Alw21I GWGCWC 3 cut(s) 363, 672, 749
Alw26I GTCTC 1 cut(s) 831
Alw44I GTGCAC 1 cut(s) 668
AlwI GGATC 2 cut(s) 287, 543
AoxI GGCC 4 cut(s) 73, 408, 504, 786
ApaLI GTGCAC 1 cut(s) 668
ApeKI GCWGC 3 cut(s) 41, 689, 825
ApoI RAATTY 2 cut(s) 283, 325
AspLEI GCGC 2 cut(s) 694, 757
AspS9I GGNCC 3 cut(s) 73, 569, 786
AsuHPI GGTGA 3 cut(s) 237, 355, 559
AsuII TTCGAA 1 cut(s) 97
AsuNHI GCTAGC 1 cut(s) 577
AvaII GGWCC 1 cut(s) 569
BaeGI GKGCMC 2 cut(s) 311, 672
BanII GRGCYC 1 cut(s) 749
BbrPI CACGTG 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 223
Bbv12I GWGCWC 3 cut(s) 363, 672, 749
BbvCI CCTCAGC 1 cut(s) 782
BbvI GCAGC 3 cut(s) 28, 676, 837
BccI CCATC 4 cut(s) 104, 178, 218, 805
BciT130I CCWGG 1 cut(s) 508
BciVI GTATCC 1 cut(s) 853
BcoDI GTCTC 1 cut(s) 831
BcuI ACTAGT 1 cut(s) 932
BfaI CTAG 3 cut(s) 65, 578, 933
BfmI CTRYAG 1 cut(s) 489
BfuI GTATCC 1 cut(s) 853
BglI GCCNNNNNGGC 1 cut(s) 407
BisI GCNGC 3 cut(s) 42, 690, 826
BlpI GCTNAGC 1 cut(s) 876
BlsI GCNGC 3 cut(s) 43, 691, 827
BmcAI AGTACT 1 cut(s) 449
Bme1390I CCNGG 1 cut(s) 508
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 3 cut(s) 73, 569, 786
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 1 cut(s) 508
BmsI GCATC 5 cut(s) 251, 388, 430, 768, 936
BmtI GCTAGC 1 cut(s) 581
BpiI GAAGAC 1 cut(s) 223
BpmI CTGGAG 2 cut(s) 42, 529
Bpu10I CCTNAGC 1 cut(s) 782
Bpu1102I GCTNAGC 1 cut(s) 876
Bpu14I TTCGAA 1 cut(s) 97
BpuEI CTTGAG 1 cut(s) 146
BsaAI YACGTR 1 cut(s) 344
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 2 cut(s) 90, 150
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 110, 156
Bse1I ACTGG 4 cut(s) 25, 618, 764, 864
Bse3DI GCAATG 1 cut(s) 711
BseBI CCWGG 1 cut(s) 508
BseDI CCNNGG 2 cut(s) 90, 150
BseGI GGATG 2 cut(s) 115, 797
BseLI CCNNNNNNNGG 3 cut(s) 91, 110, 156
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 3 cut(s) 435, 773, 890
BseNI ACTGG 4 cut(s) 25, 618, 764, 864
BseRI GAGGAG 1 cut(s) 405
BseSI GKGCMC 2 cut(s) 311, 672
BseXI GCAGC 3 cut(s) 28, 676, 837
BseYI CCCAGC 3 cut(s) 402, 518, 880
BshFI GGCC 4 cut(s) 75, 410, 506, 788
BsiHKAI GWGCWC 3 cut(s) 363, 672, 749
BslI CCNNNNNNNGG 3 cut(s) 91, 110, 156
BsmAI GTCTC 1 cut(s) 831
BsnI GGCC 4 cut(s) 75, 410, 506, 788
Bsp119I TTCGAA 1 cut(s) 97
Bsp1286I GDGCHC 4 cut(s) 311, 363, 672, 749
Bsp143I GATC 3 cut(s) 292, 535, 768
Bsp1720I GCTNAGC 1 cut(s) 876
BspACI CCGC 2 cut(s) 85, 528
BspANI GGCC 4 cut(s) 75, 410, 506, 788
BspCNI CTCAG 3 cut(s) 436, 774, 889
BspLI GGNNCC 1 cut(s) 148
BspOI GCTAGC 1 cut(s) 581
BspPI GGATC 2 cut(s) 287, 543
BspQI GCTCTTC 1 cut(s) 382
BspT104I TTCGAA 1 cut(s) 97
BsrBI CCGCTC 1 cut(s) 528
BsrDI GCAATG 1 cut(s) 711
BsrI ACTGG 4 cut(s) 25, 618, 764, 864
BssECI CCNNGG 2 cut(s) 90, 150
BssMI GATC 3 cut(s) 292, 535, 768
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 508
Bst4CI ACNGT 4 cut(s) 14, 94, 658, 842
Bst6I CTCTTC 2 cut(s) 373, 382
BstACI GRCGYC 1 cut(s) 219
BstBAI YACGTR 1 cut(s) 344
BstBI TTCGAA 1 cut(s) 97
BstC8I GCNNGC 2 cut(s) 408, 579
BstDEI CTNAG 4 cut(s) 49, 444, 782, 876
BstDSI CCRYGG 1 cut(s) 90
BstF5I GGATG 2 cut(s) 115, 797
BstHHI GCGC 2 cut(s) 694, 757
BstKTI GATC 3 cut(s) 295, 538, 771
BstMAI GTCTC 1 cut(s) 831
BstMBI GATC 3 cut(s) 292, 535, 768
BstMWI GCNNNNNNNGC 1 cut(s) 407
BstNI CCWGG 1 cut(s) 508
BstSCI CCNGG 1 cut(s) 506
BstSFI CTRYAG 1 cut(s) 489
BstSLI GKGCMC 2 cut(s) 311, 672
BstV1I GCAGC 3 cut(s) 28, 676, 837
BstV2I GAAGAC 1 cut(s) 223
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BsuI GTATCC 1 cut(s) 853
BsuRI GGCC 4 cut(s) 75, 410, 506, 788
BtgI CCRYGG 1 cut(s) 90
BtsCI GGATG 2 cut(s) 115, 797
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 3 cut(s) 237, 311, 757
Cac8I GCNNGC 2 cut(s) 408, 579
CfoI GCGC 2 cut(s) 694, 757
Cfr13I GGNCC 3 cut(s) 73, 569, 786
CseI GACGC 2 cut(s) 208, 711
Csp6I GTAC 3 cut(s) 212, 448, 643
CviAII CATG 4 cut(s) 77, 297, 330, 856
CviQI GTAC 3 cut(s) 212, 448, 643
DdeI CTNAG 4 cut(s) 49, 444, 782, 876
DpnI GATC 3 cut(s) 294, 537, 770
DpnII GATC 3 cut(s) 292, 535, 768
Eam1104I CTCTTC 2 cut(s) 373, 382
EarI CTCTTC 2 cut(s) 373, 382
Ecl136II GAGCTC 1 cut(s) 747
Eco130I CCWWGG 1 cut(s) 150
Eco147I AGGCCT 2 cut(s) 410, 506
Eco24I GRGCYC 1 cut(s) 749
Eco32I GATATC 1 cut(s) 389
Eco47I GGWCC 1 cut(s) 569
Eco53kI GAGCTC 1 cut(s) 747
Eco72I CACGTG 1 cut(s) 344
EcoICRI GAGCTC 1 cut(s) 747
EcoRI GAATTC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 506
EcoRV GATATC 1 cut(s) 389
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 749
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 4 cut(s) 80, 300, 333, 859
FatI CATG 4 cut(s) 76, 296, 329, 855
FauNDI CATATG 1 cut(s) 808
Fnu4HI GCNGC 3 cut(s) 42, 690, 826
FokI GGATG 2 cut(s) 122, 784
FriOI GRGCYC 1 cut(s) 749
Fsp4HI GCNGC 3 cut(s) 42, 690, 826
FspBI CTAG 3 cut(s) 65, 578, 933
FspI TGCGCA 2 cut(s) 693, 756
GlaI GCGC 2 cut(s) 693, 756
GluI GCNGC 3 cut(s) 42, 690, 826
GsaI CCCAGC 3 cut(s) 406, 522, 884
GsuI CTGGAG 2 cut(s) 42, 529
HaeIII GGCC 4 cut(s) 75, 410, 506, 788
HgaI GACGC 2 cut(s) 208, 711
HhaI GCGC 2 cut(s) 694, 757
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 4 cut(s) 80, 300, 333, 859
Hin6I GCGC 2 cut(s) 692, 755
HinP1I GCGC 2 cut(s) 692, 755
HindIII AAGCTT 1 cut(s) 196
HinfI GANTC 3 cut(s) 228, 425, 550
HphI GGTGA 3 cut(s) 237, 355, 559
Hpy166II GTNNAC 1 cut(s) 670
Hpy188I TCNGA 2 cut(s) 324, 766
Hpy188III TCNNGA 2 cut(s) 633, 726
Hpy8I GTNNAC 1 cut(s) 670
Hpy99I CGWCG 4 cut(s) 224, 269, 559, 727
HpyAV CCTTC 5 cut(s) 112, 185, 493, 723, 827
HpyCH4III ACNGT 4 cut(s) 14, 94, 658, 842
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 5 cut(s) 242, 453, 482, 670, 927
HpyF10VI GCNNNNNNNGC 1 cut(s) 407
HpyF3I CTNAG 4 cut(s) 49, 444, 782, 876
HpySE526I ACGT 1 cut(s) 343
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 4 cut(s) 80, 300, 333, 859
HspAI GCGC 2 cut(s) 692, 755
Kzo9I GATC 3 cut(s) 292, 535, 768
LguI GCTCTTC 1 cut(s) 382
LmnI GCTCC 1 cut(s) 146
Lsp1109I GCAGC 3 cut(s) 28, 676, 837
LweI GCATC 5 cut(s) 251, 388, 430, 768, 936
MaeI CTAG 3 cut(s) 65, 578, 933
MaeII ACGT 1 cut(s) 343
MaeIII GTNAC 2 cut(s) 16, 392
MalI GATC 3 cut(s) 294, 537, 770
MbiI CCGCTC 1 cut(s) 528
MboI GATC 3 cut(s) 292, 535, 768
MboII GAAGA 3 cut(s) 223, 360, 369
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 4 cut(s) 311, 363, 672, 749
MluCI AATT 4 cut(s) 283, 325, 513, 870
MlyI GAGTC 1 cut(s) 237
MmeI TCCRAC 1 cut(s) 744
MseI TTAA 2 cut(s) 141, 774
MslI CAYNNNNRTG 3 cut(s) 435, 675, 776
MspA1I CMGCKG 1 cut(s) 880
MspR9I CCNGG 1 cut(s) 508
MvaI CCWGG 1 cut(s) 508
MwoI GCNNNNNNNGC 1 cut(s) 407
NdeI CATATG 1 cut(s) 808
NdeII GATC 3 cut(s) 292, 535, 768
NheI GCTAGC 1 cut(s) 577
NlaIII CATG 4 cut(s) 80, 300, 333, 859
NlaIV GGNNCC 1 cut(s) 148
NsbI TGCGCA 2 cut(s) 693, 756
NspV TTCGAA 1 cut(s) 97
PceI AGGCCT 2 cut(s) 410, 506
PciSI GCTCTTC 1 cut(s) 382
PfeI GAWTC 2 cut(s) 425, 550
PflMI CCANNNNNTGG 1 cut(s) 110
PkrI GCNGC 3 cut(s) 43, 691, 827
PleI GAGTC 1 cut(s) 236
PmaCI CACGTG 1 cut(s) 344
PmlI CACGTG 1 cut(s) 344
PpsI GAGTC 1 cut(s) 236
Ppu21I YACGTR 1 cut(s) 344
Psp124BI GAGCTC 1 cut(s) 749
Psp6I CCWGG 1 cut(s) 506
PspCI CACGTG 1 cut(s) 344
PspFI CCCAGC 3 cut(s) 402, 518, 880
PspGI CCWGG 1 cut(s) 506
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 3 cut(s) 73, 569, 786
PsuI RGATCY 1 cut(s) 535
PvuII CAGCTG 1 cut(s) 880
RsaI GTAC 3 cut(s) 213, 449, 644
RsaNI GTAC 3 cut(s) 212, 448, 643
RseI CAYNNNNRTG 3 cut(s) 435, 675, 776
SacI GAGCTC 1 cut(s) 749
SapI GCTCTTC 1 cut(s) 382
SaqAI TTAA 2 cut(s) 141, 774
SatI GCNGC 3 cut(s) 42, 690, 826
Sau3AI GATC 3 cut(s) 292, 535, 768
Sau96I GGNCC 3 cut(s) 73, 569, 786
ScaI AGTACT 1 cut(s) 449
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 1 cut(s) 508
SduI GDGCHC 4 cut(s) 311, 363, 672, 749
SfaNI GCATC 5 cut(s) 251, 388, 430, 768, 936
SfcI CTRYAG 1 cut(s) 489
SfuI TTCGAA 1 cut(s) 97
SinI GGWCC 1 cut(s) 569
SmiMI CAYNNNNRTG 3 cut(s) 435, 675, 776
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SpeI ACTAGT 1 cut(s) 932
Sse9I AATT 4 cut(s) 283, 325, 513, 870
SseBI AGGCCT 2 cut(s) 410, 506
SsiI CCGC 2 cut(s) 85, 528
SspMI CTAG 3 cut(s) 65, 578, 933
SstI GAGCTC 1 cut(s) 749
StuI AGGCCT 2 cut(s) 410, 506
StyD4I CCNGG 1 cut(s) 506
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 4 cut(s) 14, 94, 658, 842
TaiI ACGT 1 cut(s) 346
TaqI TCGA 4 cut(s) 97, 222, 743, 918
TasI AATT 4 cut(s) 283, 325, 513, 870
TatI WGTACW 1 cut(s) 447
TfiI GAWTC 2 cut(s) 425, 550
Tru1I TTAA 2 cut(s) 141, 774
Tru9I TTAA 2 cut(s) 141, 774
TscAI CASTG 3 cut(s) 244, 318, 764
TseI GCWGC 3 cut(s) 41, 689, 825
TspDTI ATGAA 3 cut(s) 25, 318, 825
TspGWI ACGGA 1 cut(s) 543
TspRI CASTG 3 cut(s) 244, 318, 764
Van91I CCANNNNNTGG 1 cut(s) 110
VneI GTGCAC 1 cut(s) 668
VpaK11BI GGWCC 1 cut(s) 569
XapI RAATTY 2 cut(s) 283, 325
XspI CTAG 3 cut(s) 65, 578, 933
ZrmI AGTACT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.