Rh4CG362300

Tetraketide alpha-pyrone reductase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008036
Physical Location & Seq
Forward (+)
61909396 .. 61909893
498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG362300.1

Sequence Viewer

Length: 348 bp
ATGCCTGAATATTGTGTGACTGGAGGTACAGGGTTCATAGCTGCCTACTTGGTGAAGGCTTTGCTAGAAAATGGCCATGTTGTGCGGGCCACTGTGCGAGACCCAGAGGATTTGGGGAAGGTTGGTTATCTGCGGGAGTTGAATGGAGCCAAGGAAAGGCTTAAGCTGATCAAAGCTGATCTAATGGTGGAAGGAAGCTTTGATGAAGCCATACAAGGCGTGGATGGGGTTTTCCACACCGCATCACCAGTGCTCGTTCCGTACGATAACAATGTTCAGGCAAAGCTACTACTTGACATTGCAACTTCTTATTCAAATCAGATGTGTTTTCAATATCCTTCCCATTAA

Protein Analysis

115

Amino Acids

12.6

Weight (kDa)

5.22

Isoelectric Point (pI)

17.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 5 - 102 1.5e-10 NAD dependent epimerase/dehydratase family
NmrA PF05368 6 - 87 1.4e-10 NmrA-like family
GDP_Man_Dehyd PF16363 6 - 87 6e-09 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 6 - 85 8.2e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 8 - 86 2e-11 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 85, 133, 240
AcoI YGGCCR 1 cut(s) 73
AfaI GTAC 2 cut(s) 28, 263
AfiI CCNNNNNNNGG 1 cut(s) 156
AflII CTTAAG 1 cut(s) 161
AgsI TTSAA 3 cut(s) 142, 315, 332
AluBI AGCT 5 cut(s) 41, 166, 176, 198, 286
AluI AGCT 5 cut(s) 41, 166, 176, 198, 286
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 1 cut(s) 93
AoxI GGCC 2 cut(s) 73, 87
ApeKI GCWGC 1 cut(s) 41
AspS9I GGNCC 1 cut(s) 87
AsuHPI GGTGA 2 cut(s) 64, 237
BalI TGGCCA 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 28
BccI CCATC 1 cut(s) 218
BclI TGATCA 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 93
BfaI CTAG 1 cut(s) 65
BfrI CTTAAG 1 cut(s) 161
BisI GCNGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 1 cut(s) 148
BmsI GCATC 1 cut(s) 251
BpmI CTGGAG 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 150
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 25, 248
Bse3DI GCAATG 1 cut(s) 297
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 1 cut(s) 297
BseNI ACTGG 2 cut(s) 25, 248
BseXI GCAGC 1 cut(s) 28
BshFI GGCC 2 cut(s) 75, 89
BsiHKAI GWGCWC 1 cut(s) 255
BsiWI CGTACG 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 1 cut(s) 93
BsnI GGCC 2 cut(s) 75, 89
Bso31I GGTCTC 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 2 cut(s) 168, 178
BspACI CCGC 3 cut(s) 85, 133, 240
BspANI GGCC 2 cut(s) 75, 89
BspLI GGNNCC 1 cut(s) 148
BspTI CTTAAG 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 93
BsrDI GCAATG 1 cut(s) 297
BsrI ACTGG 2 cut(s) 25, 248
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 2 cut(s) 168, 178
BssT1I CCWWGG 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 94
BstAFI CTTAAG 1 cut(s) 161
BstC8I GCNNGC 1 cut(s) 87
BstF5I GGATG 1 cut(s) 229
BstKTI GATC 2 cut(s) 171, 181
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 2 cut(s) 168, 178
BstV1I GCAGC 1 cut(s) 28
BsuRI GGCC 2 cut(s) 75, 89
BtsCI GGATG 1 cut(s) 229
BtsIMutI CAGTG 2 cut(s) 90, 255
Cac8I GCNNGC 1 cut(s) 87
Cfr13I GGNCC 1 cut(s) 87
Csp6I GTAC 2 cut(s) 27, 262
CviAII CATG 1 cut(s) 77
CviQI GTAC 2 cut(s) 27, 262
DpnI GATC 2 cut(s) 170, 180
DpnII GATC 2 cut(s) 168, 178
EaeI YGGCCR 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 1 cut(s) 80
FaiI YATR 3 cut(s) 38, 78, 212
FatI CATG 1 cut(s) 76
FauI CCCGC 2 cut(s) 78, 126
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 1 cut(s) 42
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 1 cut(s) 42
FspBI CTAG 1 cut(s) 65
GluI GCNGC 1 cut(s) 42
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 2 cut(s) 75, 89
Hin1II CATG 1 cut(s) 80
HindIII AAGCTT 1 cut(s) 196
HphI GGTGA 2 cut(s) 64, 237
Hpy188I TCNGA 1 cut(s) 321
HpyAV CCTTC 4 cut(s) 49, 112, 185, 348
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4V TGCA 1 cut(s) 302
Hsp92II CATG 1 cut(s) 80
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 2 cut(s) 168, 178
LmnI GCTCC 1 cut(s) 146
LpnPI CCDG 6 cut(s) 6, 15, 18, 117, 261, 263
Lsp1109I GCAGC 1 cut(s) 28
LweI GCATC 1 cut(s) 251
MaeI CTAG 1 cut(s) 65
MaeIII GTNAC 1 cut(s) 16
MalI GATC 2 cut(s) 170, 180
MboI GATC 2 cut(s) 168, 178
MhlI GDGCHC 1 cut(s) 255
MlsI TGGCCA 1 cut(s) 75
MluNI TGGCCA 1 cut(s) 75
MnlI CCTC 2 cut(s) 17, 100
Mox20I TGGCCA 1 cut(s) 75
MscI TGGCCA 1 cut(s) 75
MseI TTAA 2 cut(s) 162, 346
Msp20I TGGCCA 1 cut(s) 75
MspCI CTTAAG 1 cut(s) 161
NdeII GATC 2 cut(s) 168, 178
NlaIII CATG 1 cut(s) 80
NlaIV GGNNCC 1 cut(s) 148
NmuCI GTSAC 1 cut(s) 16
PcsI WCGNNNNNNNCGW 1 cut(s) 261
Pfl23II CGTACG 1 cut(s) 261
PkrI GCNGC 1 cut(s) 43
PspLI CGTACG 1 cut(s) 261
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 1 cut(s) 87
RsaI GTAC 2 cut(s) 28, 263
RsaNI GTAC 2 cut(s) 27, 262
SaqAI TTAA 2 cut(s) 162, 346
SatI GCNGC 1 cut(s) 42
Sau3AI GATC 2 cut(s) 168, 178
Sau96I GGNCC 1 cut(s) 87
SduI GDGCHC 1 cut(s) 255
SetI ASST 7 cut(s) 28, 43, 123, 168, 178, 200, 288
SfaNI GCATC 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SsiI CCGC 3 cut(s) 85, 133, 240
SspI AATATT 1 cut(s) 11
SspMI CTAG 1 cut(s) 65
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 94
Tru1I TTAA 2 cut(s) 162, 346
Tru9I TTAA 2 cut(s) 162, 346
TscAI CASTG 2 cut(s) 97, 255
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 1 cut(s) 41
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 2 cut(s) 25, 219
TspGWI ACGGA 1 cut(s) 249
TspRI CASTG 2 cut(s) 97, 255
Vha464I CTTAAG 1 cut(s) 161
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.