RchiOBHm_Chr4g0435251

Tetraketide alpha-pyrone reductase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008036
Physical Location & Seq
Forward (+)
58884071 .. 58886459
2389 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40368

Sequence Viewer

Length: 960 bp
ATGCCTGAATATTGTGTGACTGGAGGTACAGGGTTCATAGCTGCCTACTTGGTGAAGGCTTTGCTAGAAAATGGCCATGTTGTGCGGGCCACTGTGCGAGACCCAGAGGATTTGGGGAAGGTTGGTTATCTGCGGGAGTTGAATGGAGCCAAGGAAAGGCTTAAGCTGATCAAAGCTGATCTAATGGTGGAAGGAAGCTTTGATGAAGCTATACAAGGCGTGGATGGGGTTTTCCACACCGCATCACCAGTGCTCGTTCCGTACGATAACAATGTTCAGGCGACTTTGATCGATCCTTGTATAAAGGGCACCTTGAATGTGCTGAGTTCCTGTTCGAAGGCACGCGTGAAAAGGGTTGTGCTCACATCTTCTTGTTCTTCAATAAGATACCGTTACGATGCCCAACAGGCCTCTCCTCTCAATGACTCACATTGGAGCGATCCTGAATACTGCAAACGCTTCAATCTTTGGTATGCCTATGCAAAGACAACAGCGGAAAAAGAGGCGTGGAGAATCGCAGAGGAGAGTGGAATGGATCTAGTGGTGGTGAATCCGTCTTTCGTGGTTGGTCCTCTGCTAGCACCACAACCTTCAAGCACAATACTGATGATACTGGCAATACTTAAAGGTTTAAGAGGGGAATACCCAAATACAACAGTAGGATTTGTGCACATAGATGATGTGGTAGCTACACACATTCTGGCAATGGAGGAAAGAAAAGCATCAGGCAGGCTTATATGTTCAAGTTCGGTAGCGCACTGGTCGCAGATCATTGAGATGCTCAAAGCCAAATATCCATCTTATTCATATGAAAGCAAGTGTAGCAGCAAGGAAGGAGACAATAACCCACATAGCATGGACACCACTAAAATTGCTCAACTGGGTTTTCCTCCTTTCAAAACGCTCGAAAAAATGTTTGATGACTGCATCACAAGTTTTCAAGAGAAAGGGTTTCTGTGA

Protein Analysis

319

Amino Acids

35.29

Weight (kDa)

6.19

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 5 - 241 3.4e-29 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 5 - 124 2.7e-07 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 6 - 237 1.4e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 6 - 265 6.2e-17 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 6 - 198 6.5e-14 Male sterility protein
NmrA PF05368 6 - 87 1.2e-09 NmrA-like family
NAD_binding_10 PF13460 8 - 187 1e-15 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 308
AccII CGCG 1 cut(s) 345
AciI CCGC 4 cut(s) 85, 133, 240, 494
AclWI GGATC 3 cut(s) 287, 434, 543
AcoI YGGCCR 1 cut(s) 73
AfaI GTAC 2 cut(s) 28, 263
AfiI CCNNNNNNNGG 1 cut(s) 156
AflII CTTAAG 1 cut(s) 161
AflIII ACRYGT 1 cut(s) 343
AgsI TTSAA 8 cut(s) 142, 316, 381, 463, 594, 744, 898, 941
AluBI AGCT 6 cut(s) 41, 166, 176, 198, 209, 689
AluI AGCT 6 cut(s) 41, 166, 176, 198, 209, 689
Alw21I GWGCWC 3 cut(s) 255, 363, 672
Alw26I GTCTC 2 cut(s) 93, 831
Alw44I GTGCAC 1 cut(s) 668
AlwI GGATC 3 cut(s) 287, 434, 543
AoxI GGCC 3 cut(s) 73, 87, 408
ApaLI GTGCAC 1 cut(s) 668
ApeKI GCWGC 2 cut(s) 41, 825
AspLEI GCGC 1 cut(s) 757
AspS9I GGNCC 2 cut(s) 87, 569
AsuHPI GGTGA 3 cut(s) 64, 237, 559
AsuII TTCGAA 1 cut(s) 335
AsuNHI GCTAGC 1 cut(s) 577
AvaII GGWCC 1 cut(s) 569
BaeGI GKGCMC 2 cut(s) 311, 672
BalI TGGCCA 1 cut(s) 75
BanI GGYRCC 1 cut(s) 308
Bbv12I GWGCWC 3 cut(s) 255, 363, 672
BbvI GCAGC 2 cut(s) 28, 837
BccI CCATC 2 cut(s) 218, 805
BclI TGATCA 1 cut(s) 168
BcoDI GTCTC 2 cut(s) 93, 831
BfaI CTAG 3 cut(s) 65, 539, 578
BfrI CTTAAG 1 cut(s) 161
BglI GCCNNNNNGGC 1 cut(s) 407
BisI GCNGC 2 cut(s) 42, 826
BlsI GCNGC 2 cut(s) 43, 827
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 2 cut(s) 87, 569
BmiI GGNNCC 2 cut(s) 148, 310
BmrI ACTGGG 1 cut(s) 890
BmsI GCATC 5 cut(s) 251, 388, 731, 768, 936
BmtI GCTAGC 1 cut(s) 581
BmuI ACTGGG 1 cut(s) 890
BpmI CTGGAG 1 cut(s) 42
Bpu14I TTCGAA 1 cut(s) 335
Bsa29I ATCGAT 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 150
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 5 cut(s) 25, 248, 618, 764, 885
Bse3DI GCAATG 1 cut(s) 711
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 5 cut(s) 25, 248, 618, 764, 885
BseRI GAGGAG 2 cut(s) 405, 536
BseSI GKGCMC 2 cut(s) 311, 672
BseXI GCAGC 2 cut(s) 28, 837
Bsh1236I CGCG 1 cut(s) 345
BshFI GGCC 3 cut(s) 75, 89, 410
BshNI GGYRCC 1 cut(s) 308
BshVI ATCGAT 1 cut(s) 291
BsiHKAI GWGCWC 3 cut(s) 255, 363, 672
BsiWI CGTACG 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 2 cut(s) 93, 831
BsnI GGCC 3 cut(s) 75, 89, 410
Bso31I GGTCTC 1 cut(s) 93
Bsp119I TTCGAA 1 cut(s) 335
Bsp1286I GDGCHC 4 cut(s) 255, 311, 363, 672
Bsp143I GATC 7 cut(s) 168, 178, 288, 292, 439, 535, 768
BspACI CCGC 4 cut(s) 85, 133, 240, 494
BspANI GGCC 3 cut(s) 75, 89, 410
BspCNI CTCAG 1 cut(s) 315
BspDI ATCGAT 1 cut(s) 291
BspFNI CGCG 1 cut(s) 345
BspLI GGNNCC 2 cut(s) 148, 310
BspOI GCTAGC 1 cut(s) 581
BspPI GGATC 3 cut(s) 287, 434, 543
BspT104I TTCGAA 1 cut(s) 335
BspT107I GGYRCC 1 cut(s) 308
BspTI CTTAAG 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 93
BsrDI GCAATG 1 cut(s) 711
BsrI ACTGG 5 cut(s) 25, 248, 618, 764, 885
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 7 cut(s) 168, 178, 288, 292, 439, 535, 768
BssT1I CCWWGG 1 cut(s) 150
Bst4CI ACNGT 3 cut(s) 94, 392, 658
BstAFI CTTAAG 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 335
BstC8I GCNNGC 4 cut(s) 87, 343, 579, 731
BstDEI CTNAG 1 cut(s) 323
BstF5I GGATG 1 cut(s) 229
BstFNI CGCG 1 cut(s) 345
BstHHI GCGC 1 cut(s) 757
BstKTI GATC 7 cut(s) 171, 181, 291, 295, 442, 538, 771
BstMAI GTCTC 2 cut(s) 93, 831
BstMBI GATC 7 cut(s) 168, 178, 288, 292, 439, 535, 768
BstMWI GCNNNNNNNGC 3 cut(s) 407, 763, 822
BstSLI GKGCMC 2 cut(s) 311, 672
BstUI CGCG 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 28, 837
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
Bsu15I ATCGAT 1 cut(s) 291
BsuRI GGCC 3 cut(s) 75, 89, 410
BsuTUI ATCGAT 1 cut(s) 291
BtsCI GGATG 1 cut(s) 229
BtsIMutI CAGTG 3 cut(s) 90, 255, 757
Cac8I GCNNGC 4 cut(s) 87, 343, 579, 731
CfoI GCGC 1 cut(s) 757
Cfr13I GGNCC 2 cut(s) 87, 569
ClaI ATCGAT 1 cut(s) 291
Csp6I GTAC 2 cut(s) 27, 262
CspCI CAANNNNNGTGG 2 cut(s) 853, 888
CviAII CATG 2 cut(s) 77, 856
CviQI GTAC 2 cut(s) 27, 262
DdeI CTNAG 1 cut(s) 323
DpnI GATC 7 cut(s) 170, 180, 290, 294, 441, 537, 770
DpnII GATC 7 cut(s) 168, 178, 288, 292, 439, 535, 768
EaeI YGGCCR 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 410
Eco31I GGTCTC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 569
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 2 cut(s) 80, 859
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FatI CATG 2 cut(s) 76, 855
FauI CCCGC 2 cut(s) 78, 126
FauNDI CATATG 1 cut(s) 808
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 2 cut(s) 42, 826
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 2 cut(s) 42, 826
FspBI CTAG 3 cut(s) 65, 539, 578
GlaI GCGC 1 cut(s) 756
GluI GCNGC 2 cut(s) 42, 826
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 3 cut(s) 75, 89, 410
HhaI GCGC 1 cut(s) 757
Hin1II CATG 2 cut(s) 80, 859
Hin6I GCGC 1 cut(s) 755
HinP1I GCGC 1 cut(s) 755
HindIII AAGCTT 1 cut(s) 196
HinfI GANTC 3 cut(s) 425, 513, 550
HphI GGTGA 3 cut(s) 64, 237, 559
Hpy166II GTNNAC 1 cut(s) 670
Hpy188III TCNNGA 2 cut(s) 443, 941
Hpy8I GTNNAC 1 cut(s) 670
HpyAV CCTTC 6 cut(s) 49, 112, 185, 331, 600, 827
HpyCH4III ACNGT 3 cut(s) 94, 392, 658
HpyCH4V TGCA 4 cut(s) 453, 482, 670, 927
HpyF10VI GCNNNNNNNGC 3 cut(s) 407, 763, 822
HpyF3I CTNAG 1 cut(s) 323
Hsp92II CATG 2 cut(s) 80, 859
HspAI GCGC 1 cut(s) 755
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 7 cut(s) 168, 178, 288, 292, 439, 535, 768
LmnI GCTCC 2 cut(s) 146, 435
Lsp1109I GCAGC 2 cut(s) 28, 837
LweI GCATC 5 cut(s) 251, 388, 731, 768, 936
MaeI CTAG 3 cut(s) 65, 539, 578
MaeIII GTNAC 2 cut(s) 16, 392
MalI GATC 7 cut(s) 170, 180, 290, 294, 441, 537, 770
MboI GATC 7 cut(s) 168, 178, 288, 292, 439, 535, 768
MboII GAAGA 2 cut(s) 360, 369
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 4 cut(s) 255, 311, 363, 672
MlsI TGGCCA 1 cut(s) 75
MluCI AATT 1 cut(s) 870
MluI ACGCGT 1 cut(s) 343
MluNI TGGCCA 1 cut(s) 75
MlyI GAGTC 1 cut(s) 419
Mox20I TGGCCA 1 cut(s) 75
MscI TGGCCA 1 cut(s) 75
MseI TTAA 3 cut(s) 162, 624, 632
MslI CAYNNNNRTG 2 cut(s) 675, 776
Msp20I TGGCCA 1 cut(s) 75
MspA1I CMGCKG 1 cut(s) 494
MspCI CTTAAG 1 cut(s) 161
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 3 cut(s) 407, 763, 822
NdeI CATATG 1 cut(s) 808
NdeII GATC 7 cut(s) 168, 178, 288, 292, 439, 535, 768
NheI GCTAGC 1 cut(s) 577
NlaIII CATG 2 cut(s) 80, 859
NlaIV GGNNCC 2 cut(s) 148, 310
NmuCI GTSAC 1 cut(s) 16
NspV TTCGAA 1 cut(s) 335
PceI AGGCCT 1 cut(s) 410
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PfeI GAWTC 2 cut(s) 513, 550
Pfl23II CGTACG 1 cut(s) 261
PkrI GCNGC 2 cut(s) 43, 827
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PspLI CGTACG 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 148, 310
PspPI GGNCC 2 cut(s) 87, 569
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 2 cut(s) 28, 263
RsaNI GTAC 2 cut(s) 27, 262
RseI CAYNNNNRTG 2 cut(s) 675, 776
SaqAI TTAA 3 cut(s) 162, 624, 632
SatI GCNGC 2 cut(s) 42, 826
Sau3AI GATC 7 cut(s) 168, 178, 288, 292, 439, 535, 768
Sau96I GGNCC 2 cut(s) 87, 569
SchI GAGTC 1 cut(s) 419
SduI GDGCHC 4 cut(s) 255, 311, 363, 672
SfaNI GCATC 5 cut(s) 251, 388, 731, 768, 936
SfuI TTCGAA 1 cut(s) 335
SinI GGWCC 1 cut(s) 569
SmiMI CAYNNNNRTG 2 cut(s) 675, 776
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
Sse9I AATT 1 cut(s) 870
SseBI AGGCCT 1 cut(s) 410
SsiI CCGC 4 cut(s) 85, 133, 240, 494
SspI AATATT 1 cut(s) 11
SspMI CTAG 3 cut(s) 65, 539, 578
StuI AGGCCT 1 cut(s) 410
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 3 cut(s) 94, 392, 658
TaqI TCGA 3 cut(s) 291, 335, 906
TasI AATT 1 cut(s) 870
TfiI GAWTC 2 cut(s) 513, 550
Tru1I TTAA 3 cut(s) 162, 624, 632
Tru9I TTAA 3 cut(s) 162, 624, 632
TscAI CASTG 3 cut(s) 97, 255, 764
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 2 cut(s) 41, 825
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 4 cut(s) 25, 219, 795, 825
TspGWI ACGGA 2 cut(s) 249, 543
TspRI CASTG 3 cut(s) 97, 255, 764
Vha464I CTTAAG 1 cut(s) 161
VneI GTGCAC 1 cut(s) 668
VpaK11BI GGWCC 1 cut(s) 569
XspI CTAG 3 cut(s) 65, 539, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.