AT1G33330

peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007718
Physical Location & Seq
Forward (+)
12084914 .. 12086883
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G33330.2

Sequence Viewer

Length: 774 bp
ATGGCGATCATGGCCGTTGCCGCTCTCTTTCACGCCCGCTTCCCTCTTCTCCTCCGTCGCAGTATCCCGTTACTGTCGGCTCCTACTTCTGTTATTTACAATCACAGAACTCTTCTATCCGATGGACTCAGTACTCGATTATGCTCGATTCTCCGTTATTCATCGTCTGACGGCGTCAACGGCGGAAGTAGCGGTGGCGATTTCGGTAACGATAACGATTCCGTTAGTGTTGTTTCGGATGTTCAGAGTCCTAATTACTTAAAATTCACCGATGAAGAATTGATGAAGCAATGTAGATTAGAGACATTTAGAGTTTCAGGACCTGGAGGACAACACCGGAACAAGCGTGACTCCGCCGTGCGTCTGAAACATCTCCCCACCGGAATCGTAGCTCAAGCCGTCGAAGATCGTTCACAGCACAAGAACCGTGCTTCTGCTCTAAACCGTCTTCGTACGCTCCTCGCCATTAAAGTGAGAAACAAAGTGGACATTGAAGCTTATGCTCCTCCTCCAGAGCTTCTTCAGATTCTACCTCCTAAGTCTACCATTAGAACTTCTTCTGGTTCACAGATTGGTCCCAACAATCCTAAATTTGTACCTGGAATGCAAGCTTTGCTTGATGTTATTTCTGCTTCTGACGGTTCTATCGCTGATTCTGCCAAATTGCTGGGTTTGAGCACTGGTGGCTTATCTCGTTTGATACTCTCCCATGATGGTCTTCGTATGGCTGTCAATAGCATGAGAGCAGCCAAGGGAATAAAGCCTCTCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

257

Amino Acids

27.65

Weight (kDa)

10.14

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 96 - 157 1.1e-20 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 542
AciI CCGC 5 cut(s) 21, 37, 183, 192, 354
AcoI YGGCCR 1 cut(s) 12
AcsI RAATTY 2 cut(s) 263, 590
AcuI CTGAAG 1 cut(s) 506
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 3 cut(s) 133, 454, 597
AgsI TTSAA 1 cut(s) 494
AjnI CCWGG 2 cut(s) 322, 598
AluBI AGCT 4 cut(s) 392, 497, 517, 611
AluI AGCT 4 cut(s) 392, 497, 517, 611
Alw21I GWGCWC 1 cut(s) 680
Alw26I GTCTC 1 cut(s) 296
AlwNI CAGNNNCTG 1 cut(s) 323
AoxI GGCC 1 cut(s) 12
ApeKI GCWGC 1 cut(s) 746
ApoI RAATTY 2 cut(s) 263, 590
Asp700I GAANNNNTTC 1 cut(s) 556
AspS9I GGNCC 2 cut(s) 320, 575
AsuHPI GGTGA 1 cut(s) 259
AvaII GGWCC 2 cut(s) 320, 575
BbsI GAAGAC 2 cut(s) 440, 710
Bbv12I GWGCWC 1 cut(s) 680
BbvI GCAGC 1 cut(s) 758
BccI CCATC 2 cut(s) 116, 707
BceAI ACGGC 4 cut(s) 187, 196, 341, 383
BciT130I CCWGG 2 cut(s) 324, 600
BciVI GTATCC 1 cut(s) 74
BcoDI GTCTC 1 cut(s) 296
BfuI GTATCC 1 cut(s) 74
BisI GCNGC 2 cut(s) 21, 747
BlsI GCNGC 2 cut(s) 22, 748
BmcAI AGTACT 1 cut(s) 133
Bme1390I CCNGG 2 cut(s) 324, 600
Bme18I GGWCC 2 cut(s) 320, 575
BmgT120I GGNCC 2 cut(s) 320, 575
BmiI GGNNCC 2 cut(s) 81, 577
BmrFI CCNGG 2 cut(s) 324, 600
BpiI GAAGAC 2 cut(s) 440, 710
BpmI CTGGAG 2 cut(s) 345, 495
BpuEI CTTGAG 1 cut(s) 378
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 750
BsaWI WCCGGW 2 cut(s) 336, 380
Bse1I ACTGG 1 cut(s) 685
Bse3DI GCAATG 1 cut(s) 296
BseBI CCWGG 2 cut(s) 324, 600
BseDI CCNNGG 1 cut(s) 750
BseGI GGATG 1 cut(s) 244
BseMI GCAATG 1 cut(s) 296
BseMII CTCAG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 685
BseRI GAGGAG 4 cut(s) 41, 449, 495, 498
BseXI GCAGC 1 cut(s) 758
BseYI CCCAGC 1 cut(s) 667
BshFI GGCC 1 cut(s) 14
BsiHKAI GWGCWC 1 cut(s) 680
BsiSI CCGG 2 cut(s) 337, 381
BsiWI CGTACG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 561
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 561
BsmI GAATGC 1 cut(s) 609
BsnI GGCC 1 cut(s) 14
Bsp1286I GDGCHC 1 cut(s) 680
Bsp143I GATC 2 cut(s) 6, 406
BspACI CCGC 5 cut(s) 21, 37, 183, 192, 354
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 1 cut(s) 141
BspLI GGNNCC 2 cut(s) 81, 577
BsrBI CCGCTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 296
BsrI ACTGG 1 cut(s) 685
BssECI CCNNGG 1 cut(s) 750
BssMI GATC 2 cut(s) 6, 406
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 750
Bst2UI CCWGG 2 cut(s) 324, 600
Bst4CI ACNGT 4 cut(s) 75, 428, 446, 641
Bst6I CTCTTC 2 cut(s) 51, 117
BstACI GRCGYC 1 cut(s) 174
BstAPI GCANNNNNTGC 1 cut(s) 613
BstC8I GCNNGC 2 cut(s) 37, 609
BstDEI CTNAG 2 cut(s) 128, 537
BstF5I GGATG 1 cut(s) 244
BstKTI GATC 2 cut(s) 9, 409
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 2 cut(s) 6, 406
BstMWI GCNNNNNNNGC 7 cut(s) 11, 20, 180, 189, 613, 656, 684
BstNI CCWGG 2 cut(s) 324, 600
BstSCI CCNGG 2 cut(s) 322, 598
BstV1I GCAGC 1 cut(s) 758
BstV2I GAAGAC 2 cut(s) 440, 710
BstXI CCANNNNNNTGG 1 cut(s) 667
BsuI GTATCC 1 cut(s) 74
BsuRI GGCC 1 cut(s) 14
BtsCI GGATG 1 cut(s) 244
BtsIMutI CAGTG 1 cut(s) 678
Cac8I GCNNGC 2 cut(s) 37, 609
CaiI CAGNNNCTG 1 cut(s) 323
Cfr13I GGNCC 2 cut(s) 320, 575
CseI GACGC 2 cut(s) 163, 350
Csp6I GTAC 3 cut(s) 132, 453, 596
CviAII CATG 3 cut(s) 10, 710, 739
CviQI GTAC 3 cut(s) 132, 453, 596
DdeI CTNAG 2 cut(s) 128, 537
DpnI GATC 2 cut(s) 8, 408
DpnII GATC 2 cut(s) 6, 406
EaeI YGGCCR 1 cut(s) 12
Eam1104I CTCTTC 2 cut(s) 51, 117
EarI CTCTTC 2 cut(s) 51, 117
EciI GGCGGA 2 cut(s) 198, 343
Eco130I CCWWGG 1 cut(s) 750
Eco47I GGWCC 2 cut(s) 320, 575
Eco57I CTGAAG 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 320
EcoRII CCWGG 2 cut(s) 322, 598
EcoT14I CCWWGG 1 cut(s) 750
ErhI CCWWGG 1 cut(s) 750
FaeI CATG 3 cut(s) 13, 713, 742
FaiI YATR 6 cut(s) 11, 142, 501, 711, 725, 740
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 1 cut(s) 561
FatI CATG 3 cut(s) 9, 709, 738
FauI CCCGC 1 cut(s) 44
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 2 cut(s) 21, 747
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 2 cut(s) 21, 747
GluI GCNGC 2 cut(s) 21, 747
GsaI CCCAGC 1 cut(s) 671
GsuI CTGGAG 2 cut(s) 345, 495
HaeIII GGCC 1 cut(s) 14
HapII CCGG 2 cut(s) 337, 381
HgaI GACGC 2 cut(s) 163, 350
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 3 cut(s) 13, 713, 742
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HindIII AAGCTT 2 cut(s) 495, 609
HinfI GANTC 8 cut(s) 126, 148, 218, 247, 350, 384, 526, 653
HpaII CCGG 2 cut(s) 337, 381
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 5 cut(s) 178, 413, 487, 543, 566
Hpy188I TCNGA 7 cut(s) 121, 169, 238, 246, 366, 525, 637
Hpy188III TCNNGA 2 cut(s) 318, 512
Hpy8I GTNNAC 5 cut(s) 178, 413, 487, 543, 566
Hpy99I CGWCG 2 cut(s) 60, 404
HpyCH4III ACNGT 4 cut(s) 75, 428, 446, 641
HpyCH4V TGCA 1 cut(s) 607
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 20, 180, 189, 613, 656, 684
HpyF3I CTNAG 2 cut(s) 128, 537
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 3 cut(s) 13, 713, 742
Kzo9I GATC 2 cut(s) 6, 406
LmnI GCTCC 3 cut(s) 85, 462, 508
Lsp1109I GCAGC 1 cut(s) 758
MaeIII GTNAC 3 cut(s) 69, 206, 347
MalI GATC 2 cut(s) 8, 408
MbiI CCGCTC 1 cut(s) 23
MboI GATC 2 cut(s) 6, 406
MboII GAAGA 8 cut(s) 38, 104, 287, 416, 440, 512, 549, 710
MhlI GDGCHC 1 cut(s) 680
MluCI AATT 5 cut(s) 253, 263, 278, 590, 662
MlyI GAGTC 3 cut(s) 120, 256, 344
MnlI CCTC 8 cut(s) 54, 62, 320, 470, 516, 519, 543, 774
MroXI GAANNNNTTC 1 cut(s) 556
MseI TTAA 2 cut(s) 260, 468
MslI CAYNNNNRTG 1 cut(s) 470
MspI CCGG 2 cut(s) 337, 381
MspR9I CCNGG 2 cut(s) 324, 600
Mva1269I GAATGC 1 cut(s) 609
MvaI CCWGG 2 cut(s) 324, 600
MwoI GCNNNNNNNGC 7 cut(s) 11, 20, 180, 189, 613, 656, 684
NdeII GATC 2 cut(s) 6, 406
NlaIII CATG 3 cut(s) 13, 713, 742
NlaIV GGNNCC 2 cut(s) 81, 577
NmuCI GTSAC 1 cut(s) 347
PctI GAATGC 1 cut(s) 609
PdmI GAANNNNTTC 1 cut(s) 556
PfeI GAWTC 5 cut(s) 148, 218, 384, 526, 653
Pfl23II CGTACG 1 cut(s) 452
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 2 cut(s) 22, 748
PleI GAGTC 3 cut(s) 120, 255, 344
PpsI GAGTC 3 cut(s) 120, 255, 344
PpuMI RGGWCCY 1 cut(s) 320
Psp5II RGGWCCY 1 cut(s) 320
Psp6I CCWGG 2 cut(s) 322, 598
PspFI CCCAGC 1 cut(s) 667
PspGI CCWGG 2 cut(s) 322, 598
PspLI CGTACG 1 cut(s) 452
PspN4I GGNNCC 2 cut(s) 81, 577
PspPI GGNCC 2 cut(s) 320, 575
PspPPI RGGWCCY 1 cut(s) 320
PstNI CAGNNNCTG 1 cut(s) 323
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 3 cut(s) 133, 454, 597
RsaNI GTAC 3 cut(s) 132, 453, 596
RseI CAYNNNNRTG 1 cut(s) 470
SaqAI TTAA 2 cut(s) 260, 468
SatI GCNGC 2 cut(s) 21, 747
Sau3AI GATC 2 cut(s) 6, 406
Sau96I GGNCC 2 cut(s) 320, 575
ScaI AGTACT 1 cut(s) 133
SchI GAGTC 3 cut(s) 120, 256, 344
ScrFI CCNGG 2 cut(s) 324, 600
SduI GDGCHC 1 cut(s) 680
SetI ASST 7 cut(s) 325, 394, 499, 519, 535, 601, 613
SinI GGWCC 2 cut(s) 320, 575
SmiMI CAYNNNNRTG 1 cut(s) 470
SmlI CTYRAG 1 cut(s) 393
SmoI CTYRAG 1 cut(s) 393
Sse9I AATT 5 cut(s) 253, 263, 278, 590, 662
SsiI CCGC 5 cut(s) 21, 37, 183, 192, 354
StyD4I CCNGG 2 cut(s) 322, 598
StyI CCWWGG 1 cut(s) 750
TaaI ACNGT 4 cut(s) 75, 428, 446, 641
TaqI TCGA 3 cut(s) 136, 146, 402
TasI AATT 5 cut(s) 253, 263, 278, 590, 662
TatI WGTACW 1 cut(s) 131
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 5 cut(s) 148, 218, 384, 526, 653
Tru1I TTAA 2 cut(s) 260, 468
Tru9I TTAA 2 cut(s) 260, 468
TscAI CASTG 1 cut(s) 685
TseFI GTSAC 1 cut(s) 347
TseI GCWGC 1 cut(s) 746
Tsp45I GTSAC 1 cut(s) 347
TspDTI ATGAA 3 cut(s) 150, 288, 299
TspGWI ACGGA 3 cut(s) 44, 143, 211
TspRI CASTG 1 cut(s) 685
Tth111I GACNNNGTC 1 cut(s) 173
VpaK11BI GGWCC 2 cut(s) 320, 575
XapI RAATTY 2 cut(s) 263, 590
XmiI GTMKAC 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 556
ZrmI AGTACT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.