RchiOBHm_Chr6g0281061

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007718
Physical Location & Seq
Reverse (-)
44444247 .. 44446975
2729 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25207

Sequence Viewer

Length: 828 bp
ATGAGAATGGCAATGGCAGGGTCACTGTGGAAGTCATCGTGTTCATGTTCATATACGTGTTCATTGGTTAGAGTAATAGGAGTTTCAAGAACAAGTAGTGGTGCAGCGGCTGCTATACTTCATGCATCATCTCTATTTCCACCTCCATCCATGGCCTTAAAAATACAAAGAAAAAGTAGCTTAACCAAGTGGAGGTGGTCTCAACAGCAACCACAATTACCAGAAGACAGGAACAGCAATGCCTTCTTCTTCATACGGAGCTCTAAATACGGAACCAATAGTATTAGCACTGATGGCGGAAAGGGATATTTGGAATCTACAGACCAAGAGTTGATGAGCCAGTGCGAAATGGGCACTTTCAAGACGTCAGGGCCAGGGGGTCAGCACCGCAACAAGCGAGAGTCTGCAGTACGCCTTAAGCATCTCCCAACTGGTATTATTGCACAGGCTTCTGAGGATCGATCCCAGCACATGAATCGTGCCTCAGCTTTATCTCGCCTGCGCACTCTTATAGCTCTCAAAGTCAGGAACGCTGTGGATCTCGATGGTTATTCCCCTCCTCGAGAGCTTCTTCAAATTCTTCCCAAATCATCTTCCATTAGAACATCAGATATTGGTAACCAAATTGGACCGAACAATCCGAAATTTGTTTACGGAATGCAAGCTCTGTTGGATCTCCTTTATGCAGTTGATGGTTCTGTCTCAGAGGCAGCAAAGTTTCTGGGGTTGAGCACAGGTGCACTGTCTCGATTAATACTCTCTGATGATTCTCTCCGATTGGCAGTCAATGAGCTTAGGGCTTCTGAGGACATGAAGCCTTTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

275

Amino Acids

30.04

Weight (kDa)

9.85

Isoelectric Point (pI)

48.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 113 - 172 3.3e-19 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 368
Acc16I TGCGCA 1 cut(s) 503
AciI CCGC 3 cut(s) 107, 297, 388
AclWI GGATC 4 cut(s) 456, 465, 546, 681
AcsI RAATTY 2 cut(s) 576, 644
AcyI GRCGYC 1 cut(s) 365
AfaI GTAC 1 cut(s) 411
AfiI CCNNNNNNNGG 1 cut(s) 192
AflII CTTAAG 1 cut(s) 416
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 3 cut(s) 87, 361, 575
AjnI CCWGG 1 cut(s) 373
AluBI AGCT 7 cut(s) 180, 261, 488, 515, 568, 665, 793
AluI AGCT 7 cut(s) 180, 261, 488, 515, 568, 665, 793
Alw21I GWGCWC 3 cut(s) 263, 734, 742
Alw26I GTCTC 3 cut(s) 204, 706, 750
Alw44I GTGCAC 1 cut(s) 738
AlwI GGATC 4 cut(s) 456, 465, 546, 681
AlwNI CAGNNNCTG 1 cut(s) 110
Ama87I CYCGRG 1 cut(s) 561
AoxI GGCC 2 cut(s) 153, 371
ApaLI GTGCAC 1 cut(s) 738
ApeKI GCWGC 3 cut(s) 104, 110, 710
ApoI RAATTY 2 cut(s) 576, 644
AseI ATTAAT 1 cut(s) 752
AspLEI GCGC 1 cut(s) 504
AspS9I GGNCC 2 cut(s) 371, 629
AvaI CYCGRG 1 cut(s) 561
AvaII GGWCC 1 cut(s) 629
BaeGI GKGCMC 2 cut(s) 356, 742
BanII GRGCYC 1 cut(s) 263
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 3 cut(s) 263, 734, 742
BbvCI CCTCAGC 1 cut(s) 484
BbvI GCAGC 3 cut(s) 97, 116, 722
BccI CCATC 4 cut(s) 154, 287, 539, 686
BcgI CGANNNNNNTGC 2 cut(s) 458, 492
BciT130I CCWGG 1 cut(s) 375
BcoDI GTCTC 3 cut(s) 204, 706, 750
BfmI CTRYAG 2 cut(s) 318, 405
BfrI CTTAAG 1 cut(s) 416
BisI GCNGC 4 cut(s) 105, 108, 111, 711
BlsI GCNGC 4 cut(s) 106, 109, 112, 712
Bme1390I CCNGG 1 cut(s) 375
Bme18I GGWCC 1 cut(s) 629
BmeT110I CYCGRG 1 cut(s) 561
BmgT120I GGNCC 2 cut(s) 371, 629
BmiI GGNNCC 1 cut(s) 274
BmrFI CCNGG 1 cut(s) 375
BmsI GCATC 2 cut(s) 134, 430
BpiI GAAGAC 1 cut(s) 231
BplI GAGNNNNNCTC 2 cut(s) 184, 216
Bpu10I CCTNAGC 2 cut(s) 484, 794
Bsa29I ATCGAT 1 cut(s) 460
BsaAI YACGTR 1 cut(s) 57
BsaHI GRCGYC 1 cut(s) 365
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 2 cut(s) 150, 374
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse1I ACTGG 2 cut(s) 340, 436
Bse3DI GCAATG 2 cut(s) 18, 244
BseBI CCWGG 1 cut(s) 375
BseCI ATCGAT 1 cut(s) 460
BseDI CCNNGG 2 cut(s) 150, 374
BseGI GGATG 1 cut(s) 146
BseLI CCNNNNNNNGG 1 cut(s) 192
BseMI GCAATG 2 cut(s) 18, 244
BseMII CTCAG 4 cut(s) 444, 498, 717, 795
BseNI ACTGG 2 cut(s) 340, 436
BseRI GAGGAG 1 cut(s) 549
BseSI GKGCMC 2 cut(s) 356, 742
BseXI GCAGC 3 cut(s) 97, 116, 722
BseYI CCCAGC 1 cut(s) 465
BsgI GTGCAG 1 cut(s) 123
BshFI GGCC 2 cut(s) 155, 373
BshVI ATCGAT 1 cut(s) 460
BsiHKAI GWGCWC 3 cut(s) 263, 734, 742
BsiHKCI CYCGRG 1 cut(s) 561
BslI CCNNNNNNNGG 1 cut(s) 192
BsmAI GTCTC 3 cut(s) 204, 706, 750
BsmI GAATGC 1 cut(s) 663
BsnI GGCC 2 cut(s) 155, 373
Bso31I GGTCTC 1 cut(s) 204
BsoBI CYCGRG 1 cut(s) 561
Bsp1286I GDGCHC 4 cut(s) 263, 356, 734, 742
Bsp143I GATC 4 cut(s) 457, 461, 538, 673
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 3 cut(s) 107, 297, 388
BspANI GGCC 2 cut(s) 155, 373
BspCNI CTCAG 4 cut(s) 445, 497, 716, 796
BspDI ATCGAT 1 cut(s) 460
BspLI GGNNCC 1 cut(s) 274
BspMAI CTGCAG 1 cut(s) 409
BspPI GGATC 4 cut(s) 456, 465, 546, 681
BspTI CTTAAG 1 cut(s) 416
BspTNI GGTCTC 1 cut(s) 204
BsrDI GCAATG 2 cut(s) 18, 244
BsrI ACTGG 2 cut(s) 340, 436
BssECI CCNNGG 2 cut(s) 150, 374
BssMI GATC 4 cut(s) 457, 461, 538, 673
BssNI GRCGYC 1 cut(s) 365
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 375
Bst4CI ACNGT 2 cut(s) 27, 744
BstACI GRCGYC 1 cut(s) 365
BstAFI CTTAAG 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 110
BstBAI YACGTR 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 500, 663
BstDEI CTNAG 5 cut(s) 453, 484, 703, 794, 804
BstDSI CCRYGG 1 cut(s) 150
BstEII GGTNACC 1 cut(s) 617
BstF5I GGATG 1 cut(s) 146
BstHHI GCGC 1 cut(s) 504
BstKTI GATC 4 cut(s) 460, 464, 541, 676
BstMAI GTCTC 3 cut(s) 204, 706, 750
BstMBI GATC 4 cut(s) 457, 461, 538, 673
BstMWI GCNNNNNNNGC 3 cut(s) 110, 294, 351
BstNI CCWGG 1 cut(s) 375
BstPI GGTNACC 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 373
BstSFI CTRYAG 2 cut(s) 318, 405
BstSLI GKGCMC 2 cut(s) 356, 742
BstV1I GCAGC 3 cut(s) 97, 116, 722
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 2 cut(s) 538, 673
BstYI RGATCY 2 cut(s) 538, 673
Bsu15I ATCGAT 1 cut(s) 460
BsuRI GGCC 2 cut(s) 155, 373
BsuTUI ATCGAT 1 cut(s) 460
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 1 cut(s) 146
BtsIMutI CAGTG 4 cut(s) 23, 288, 347, 740
Cac8I GCNNGC 2 cut(s) 500, 663
CaiI CAGNNNCTG 1 cut(s) 110
CfoI GCGC 1 cut(s) 504
Cfr13I GGNCC 2 cut(s) 371, 629
ClaI ATCGAT 1 cut(s) 460
Csp6I GTAC 1 cut(s) 410
CviAII CATG 5 cut(s) 45, 122, 151, 472, 811
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 5 cut(s) 453, 484, 703, 794, 804
DpnI GATC 4 cut(s) 459, 463, 540, 675
DpnII GATC 4 cut(s) 457, 461, 538, 673
EciI GGCGGA 1 cut(s) 312
Ecl136II GAGCTC 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 263
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 629
Eco53kI GAGCTC 1 cut(s) 261
Eco88I CYCGRG 1 cut(s) 561
Eco91I GGTNACC 1 cut(s) 617
EcoICRI GAGCTC 1 cut(s) 261
EcoO65I GGTNACC 1 cut(s) 617
EcoRII CCWGG 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 127
EcoT38I GRGCYC 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 5 cut(s) 48, 125, 154, 475, 814
FatI CATG 5 cut(s) 44, 121, 150, 471, 810
Fnu4HI GCNGC 4 cut(s) 105, 108, 111, 711
FokI GGATG 1 cut(s) 133
FriOI GRGCYC 1 cut(s) 263
Fsp4HI GCNGC 4 cut(s) 105, 108, 111, 711
FspI TGCGCA 1 cut(s) 503
GlaI GCGC 1 cut(s) 503
GluI GCNGC 4 cut(s) 105, 108, 111, 711
GsaI CCCAGC 1 cut(s) 469
HaeIII GGCC 2 cut(s) 155, 373
HhaI GCGC 1 cut(s) 504
Hin1I GRCGYC 1 cut(s) 365
Hin1II CATG 5 cut(s) 48, 125, 154, 475, 814
Hin6I GCGC 1 cut(s) 502
HinP1I GCGC 1 cut(s) 502
HinfI GANTC 4 cut(s) 314, 401, 475, 767
Hpy166II GTNNAC 2 cut(s) 652, 740
Hpy188I TCNGA 7 cut(s) 454, 610, 642, 706, 763, 776, 805
Hpy188III TCNNGA 6 cut(s) 87, 361, 526, 542, 563, 747
Hpy8I GTNNAC 2 cut(s) 652, 740
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 2 cut(s) 27, 744
HpyCH4IV ACGT 2 cut(s) 56, 365
HpyCH4V TGCA 7 cut(s) 104, 125, 407, 443, 661, 686, 740
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 294, 351
HpyF3I CTNAG 5 cut(s) 453, 484, 703, 794, 804
HpySE526I ACGT 2 cut(s) 56, 365
Hsp92I GRCGYC 1 cut(s) 365
Hsp92II CATG 5 cut(s) 48, 125, 154, 475, 814
HspAI GCGC 1 cut(s) 502
Kzo9I GATC 4 cut(s) 457, 461, 538, 673
LmnI GCTCC 1 cut(s) 258
Lsp1109I GCAGC 3 cut(s) 97, 116, 722
LweI GCATC 2 cut(s) 134, 430
MaeII ACGT 2 cut(s) 56, 365
MaeIII GTNAC 2 cut(s) 21, 617
MalI GATC 4 cut(s) 459, 463, 540, 675
MboI GATC 4 cut(s) 457, 461, 538, 673
MboII GAAGA 6 cut(s) 236, 238, 241, 563, 572, 585
MflI RGATCY 2 cut(s) 538, 673
MhlI GDGCHC 4 cut(s) 263, 356, 734, 742
MluCI AATT 4 cut(s) 215, 576, 624, 644
MlyI GAGTC 1 cut(s) 410
MmeI TCCRAC 1 cut(s) 651
MnlI CCTC 9 cut(s) 153, 186, 448, 493, 567, 570, 700, 799, 816
Mph1103I ATGCAT 1 cut(s) 127
MseI TTAA 4 cut(s) 158, 182, 417, 752
MslI CAYNNNNRTG 1 cut(s) 55
MspA1I CMGCKG 1 cut(s) 107
MspCI CTTAAG 1 cut(s) 416
MspR9I CCNGG 1 cut(s) 375
Mva1269I GAATGC 1 cut(s) 663
MvaI CCWGG 1 cut(s) 375
MwoI GCNNNNNNNGC 3 cut(s) 110, 294, 351
NcoI CCATGG 1 cut(s) 150
NdeII GATC 4 cut(s) 457, 461, 538, 673
NlaIII CATG 5 cut(s) 48, 125, 154, 475, 814
NlaIV GGNNCC 1 cut(s) 274
NmuCI GTSAC 1 cut(s) 21
NsbI TGCGCA 1 cut(s) 503
NsiI ATGCAT 1 cut(s) 127
PaeR7I CTCGAG 1 cut(s) 561
PctI GAATGC 1 cut(s) 663
PfeI GAWTC 3 cut(s) 314, 475, 767
PkrI GCNGC 4 cut(s) 106, 109, 112, 712
PleI GAGTC 1 cut(s) 409
PpsI GAGTC 1 cut(s) 409
Ppu21I YACGTR 1 cut(s) 57
PshBI ATTAAT 1 cut(s) 752
Psp124BI GAGCTC 1 cut(s) 263
Psp6I CCWGG 1 cut(s) 373
PspEI GGTNACC 1 cut(s) 617
PspFI CCCAGC 1 cut(s) 465
PspGI CCWGG 1 cut(s) 373
PspN4I GGNNCC 1 cut(s) 274
PspPI GGNCC 2 cut(s) 371, 629
PstI CTGCAG 1 cut(s) 409
PstNI CAGNNNCTG 1 cut(s) 110
PsuI RGATCY 2 cut(s) 538, 673
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
RseI CAYNNNNRTG 1 cut(s) 55
SacI GAGCTC 1 cut(s) 263
SaqAI TTAA 4 cut(s) 158, 182, 417, 752
SatI GCNGC 4 cut(s) 105, 108, 111, 711
Sau3AI GATC 4 cut(s) 457, 461, 538, 673
Sau96I GGNCC 2 cut(s) 371, 629
SchI GAGTC 1 cut(s) 410
ScrFI CCNGG 1 cut(s) 375
SduI GDGCHC 4 cut(s) 263, 356, 734, 742
SfaNI GCATC 2 cut(s) 134, 430
SfcI CTRYAG 2 cut(s) 318, 405
Sfr274I CTCGAG 1 cut(s) 561
SinI GGWCC 1 cut(s) 629
SlaI CTCGAG 1 cut(s) 561
SmiMI CAYNNNNRTG 1 cut(s) 55
SmlI CTYRAG 2 cut(s) 416, 561
SmoI CTYRAG 2 cut(s) 416, 561
Sse9I AATT 4 cut(s) 215, 576, 624, 644
SsiI CCGC 3 cut(s) 107, 297, 388
SstI GAGCTC 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 373
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 27, 744
TaiI ACGT 2 cut(s) 59, 368
TaqI TCGA 4 cut(s) 460, 543, 562, 748
TaqII GACCGA 1 cut(s) 646
TasI AATT 4 cut(s) 215, 576, 624, 644
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 3 cut(s) 314, 475, 767
Tru1I TTAA 4 cut(s) 158, 182, 417, 752
Tru9I TTAA 4 cut(s) 158, 182, 417, 752
TscAI CASTG 4 cut(s) 30, 295, 347, 747
TseFI GTSAC 1 cut(s) 21
TseI GCWGC 3 cut(s) 104, 110, 710
Tsp45I GTSAC 1 cut(s) 21
TspDTI ATGAA 7 cut(s) 33, 39, 51, 110, 241, 488, 827
TspGWI ACGGA 3 cut(s) 271, 285, 669
TspRI CASTG 4 cut(s) 30, 295, 347, 747
Vha464I CTTAAG 1 cut(s) 416
VneI GTGCAC 1 cut(s) 738
VpaK11BI GGWCC 1 cut(s) 629
VspI ATTAAT 1 cut(s) 752
XapI RAATTY 2 cut(s) 576, 644
XhoI CTCGAG 1 cut(s) 561
ZraI GACGTC 1 cut(s) 366
Zsp2I ATGCAT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.