Rorug06G0138700

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007718
Physical Location & Seq
Reverse (-)
19614972 .. 19616060
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0138700.1

Sequence Viewer

Length: 666 bp
ATGGGTTATCCGATCGGGATCGGGTACAGGTTTCATCCCACAGATGAAGAACTTGTGGGTTACTTTCTCCAGCAAAGGGCCAAGATGGGCAATCAGTTCCACTCGAATGAGATCTCCGAGTATTCGGAGTTTTACGGCCAAGAAGAACCCTGGGTTGTTTGGGACAAGAACGGTGGTTCCAGCGTGGATGATGGAGAGCCTCTCTTTTTCTTCACCAAACGCAAGAAGTTGAACCCCAATGGCAAGCGGTTTGATCGCAAAGTAGGCTCGGGAACGTGGAGCGGGCAGTTTTCCAGAGAGGTTGTGGCCGAGGATAGAGACACCGGATGTCGTATTACTGGGATGAGGAGGGAATTTAGGTACGAGGGTGGGTGTGATCCTGAACAAAACAACTCTTGGTTGATGCAAGAGTACGAGTTGTGTGCCACTGATGTCTTGGTGTTGTGCACCTTGAAGAAGAACCCAAGAAAAGCTAGTAGTGCGAAGAGGAAGAGGATGGCCTGTCCGGTAACAAAAACCAAGGCAGTGAGATCGGAAAAGGCTGAGACTTGTGAGGAACAACAATCCACCATCTGTGTCGATCTGGAAGAGACCAATTCCGAGAGTCGAGATCATCAGTTGTTGCAAGTTGTTGATGAAGATTATCTTCCTGAGCTCTTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

221

Amino Acids

25.5

Weight (kDa)

5.32

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 7 - 140 1.5e-23 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 282
AciI CCGC 2 cut(s) 247, 282
AclWI GGATC 2 cut(s) 26, 371
AcoI YGGCCR 2 cut(s) 136, 306
AcsI RAATTY 1 cut(s) 353
AfaI GTAC 3 cut(s) 26, 362, 413
AfiI CCNNNNNNNGG 1 cut(s) 76
AgsI TTSAA 2 cut(s) 232, 454
AjnI CCWGG 1 cut(s) 149
AloI GAACNNNNNNTCC 2 cut(s) 161, 193
AluBI AGCT 2 cut(s) 473, 655
AluI AGCT 2 cut(s) 473, 655
Alw21I GWGCWC 2 cut(s) 449, 657
Alw26I GTCTC 3 cut(s) 312, 539, 584
Alw44I GTGCAC 1 cut(s) 445
AlwI GGATC 2 cut(s) 26, 371
Ama87I CYCGRG 1 cut(s) 268
AoxI GGCC 4 cut(s) 78, 136, 306, 498
ApaLI GTGCAC 1 cut(s) 445
ApoI RAATTY 1 cut(s) 353
AspS9I GGNCC 1 cut(s) 78
AsuHPI GGTGA 1 cut(s) 205
AvaI CYCGRG 1 cut(s) 268
BaeGI GKGCMC 1 cut(s) 449
BaeI ACNNNNGTAYC 2 cut(s) 352, 385
BanII GRGCYC 1 cut(s) 657
Bbv12I GWGCWC 2 cut(s) 449, 657
BccI CCATC 4 cut(s) 79, 185, 490, 578
BceAI ACGGC 1 cut(s) 151
BcgI CGANNNNNNTGC 4 cut(s) 404, 438, 513, 547
BciT130I CCWGG 1 cut(s) 151
BcoDI GTCTC 3 cut(s) 312, 539, 584
BfaI CTAG 1 cut(s) 474
BglII AGATCT 1 cut(s) 111
Bme1390I CCNGG 1 cut(s) 151
BmeT110I CYCGRG 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 78
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 1 cut(s) 151
BmrI ACTGGG 1 cut(s) 348
BmsI GCATC 1 cut(s) 393
BmuI ACTGGG 1 cut(s) 348
BplI GAGNNNNNCTC 2 cut(s) 186, 218
BpmI CTGGAG 1 cut(s) 53
Bpu10I CCTNAGC 1 cut(s) 651
BsaBI GATNNNNATC 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 584
BsaJI CCNNGG 4 cut(s) 149, 150, 309, 519
BsaWI WCCGGW 2 cut(s) 323, 505
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 17
BseBI CCWGG 1 cut(s) 151
BseDI CCNNGG 4 cut(s) 149, 150, 309, 519
BseGI GGATG 5 cut(s) 34, 193, 332, 348, 501
BseJI GATNNNNATC 1 cut(s) 17
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMII CTCAG 2 cut(s) 534, 642
BseNI ACTGG 1 cut(s) 343
BseRI GAGGAG 1 cut(s) 361
BseSI GKGCMC 1 cut(s) 449
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 4 cut(s) 80, 138, 308, 500
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 2 cut(s) 449, 657
BsiHKCI CYCGRG 1 cut(s) 268
BsiSI CCGG 2 cut(s) 324, 506
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 3 cut(s) 312, 539, 584
BsmFI GGGAC 1 cut(s) 176
BsnI GGCC 4 cut(s) 80, 138, 308, 500
Bso31I GGTCTC 1 cut(s) 584
BsoBI CYCGRG 1 cut(s) 268
Bsp1286I GDGCHC 2 cut(s) 449, 657
Bsp143I GATC 8 cut(s) 12, 18, 111, 253, 376, 530, 580, 610
BspACI CCGC 2 cut(s) 247, 282
BspANI GGCC 4 cut(s) 80, 138, 308, 500
BspCNI CTCAG 2 cut(s) 535, 643
BspLI GGNNCC 1 cut(s) 178
BspPI GGATC 2 cut(s) 26, 371
BspTNI GGTCTC 1 cut(s) 584
BsrBI CCGCTC 1 cut(s) 282
BsrI ACTGG 1 cut(s) 343
BssECI CCNNGG 4 cut(s) 149, 150, 309, 519
BssMI GATC 8 cut(s) 12, 18, 111, 253, 376, 530, 580, 610
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 1 cut(s) 151
Bst4CI ACNGT 1 cut(s) 173
Bst6I CTCTTC 3 cut(s) 479, 485, 582
BstC8I GCNNGC 2 cut(s) 245, 284
BstDEI CTNAG 3 cut(s) 543, 651, 663
BstF5I GGATG 5 cut(s) 34, 193, 332, 348, 501
BstKTI GATC 8 cut(s) 15, 21, 114, 256, 379, 533, 583, 613
BstMAI GTCTC 3 cut(s) 312, 539, 584
BstMBI GATC 8 cut(s) 12, 18, 111, 253, 376, 530, 580, 610
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 2 cut(s) 264, 479
BstNI CCWGG 1 cut(s) 151
BstSCI CCNGG 1 cut(s) 149
BstSLI GKGCMC 1 cut(s) 449
BstX2I RGATCY 1 cut(s) 111
BstYI RGATCY 1 cut(s) 111
BsuRI GGCC 4 cut(s) 80, 138, 308, 500
BtsCI GGATG 5 cut(s) 34, 193, 332, 348, 501
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 2 cut(s) 426, 531
Cac8I GCNNGC 2 cut(s) 245, 284
Cfr13I GGNCC 1 cut(s) 78
Csp6I GTAC 3 cut(s) 25, 361, 412
CspCI CAANNNNNGTGG 2 cut(s) 154, 189
CviJI RGCY 9 cut(s) 80, 138, 199, 267, 308, 473, 500, 542, 655
CviKI_1 RGCY 9 cut(s) 80, 138, 199, 267, 308, 473, 500, 542, 655
CviQI GTAC 3 cut(s) 25, 361, 412
DdeI CTNAG 3 cut(s) 543, 651, 663
DpnI GATC 8 cut(s) 14, 20, 113, 255, 378, 532, 582, 612
DpnII GATC 8 cut(s) 12, 18, 111, 253, 376, 530, 580, 610
EaeI YGGCCR 2 cut(s) 136, 306
Eam1104I CTCTTC 3 cut(s) 479, 485, 582
EarI CTCTTC 3 cut(s) 479, 485, 582
Ecl136II GAGCTC 1 cut(s) 655
Eco130I CCWWGG 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 657
Eco31I GGTCTC 1 cut(s) 584
Eco53kI GAGCTC 1 cut(s) 655
Eco88I CYCGRG 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 655
EcoRII CCWGG 1 cut(s) 149
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 519
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FaqI GGGAC 1 cut(s) 176
FauI CCCGC 1 cut(s) 275
FokI GGATG 5 cut(s) 21, 200, 339, 355, 508
FriOI GRGCYC 1 cut(s) 657
FspBI CTAG 1 cut(s) 474
GsuI CTGGAG 1 cut(s) 53
HaeIII GGCC 4 cut(s) 80, 138, 308, 500
HapII CCGG 2 cut(s) 324, 506
HinfI GANTC 1 cut(s) 604
HpaII CCGG 2 cut(s) 324, 506
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 1 cut(s) 447
Hpy188I TCNGA 5 cut(s) 12, 118, 127, 535, 601
Hpy188III TCNNGA 7 cut(s) 16, 270, 294, 380, 584, 608, 650
Hpy8I GTNNAC 1 cut(s) 447
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4IV ACGT 1 cut(s) 275
HpyCH4V TGCA 3 cut(s) 406, 447, 625
HpyF10VI GCNNNNNNNGC 2 cut(s) 264, 479
HpyF3I CTNAG 3 cut(s) 543, 651, 663
HpySE526I ACGT 1 cut(s) 275
Kzo9I GATC 8 cut(s) 12, 18, 111, 253, 376, 530, 580, 610
LmnI GCTCC 1 cut(s) 279
LweI GCATC 1 cut(s) 393
MaeI CTAG 1 cut(s) 474
MaeII ACGT 1 cut(s) 275
MaeIII GTNAC 2 cut(s) 59, 508
MalI GATC 8 cut(s) 14, 20, 113, 255, 378, 532, 582, 612
MbiI CCGCTC 1 cut(s) 282
MboI GATC 8 cut(s) 12, 18, 111, 253, 376, 530, 580, 610
MflI RGATCY 1 cut(s) 111
MhlI GDGCHC 2 cut(s) 449, 657
MluCI AATT 2 cut(s) 353, 595
MlyI GAGTC 1 cut(s) 613
MnlI CCTC 9 cut(s) 210, 292, 304, 339, 342, 358, 480, 486, 547
MslI CAYNNNNRTG 1 cut(s) 105
MspI CCGG 2 cut(s) 324, 506
MspR9I CCNGG 1 cut(s) 151
MvaI CCWGG 1 cut(s) 151
MwoI GCNNNNNNNGC 2 cut(s) 264, 479
NdeII GATC 8 cut(s) 12, 18, 111, 253, 376, 530, 580, 610
NlaIV GGNNCC 1 cut(s) 178
NmeAIII GCCGAG 1 cut(s) 334
PasI CCCWGGG 1 cut(s) 150
Ple19I CGATCG 1 cut(s) 15
PleI GAGTC 1 cut(s) 612
PpsI GAGTC 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 657
Psp6I CCWGG 1 cut(s) 149
PspGI CCWGG 1 cut(s) 149
PspN4I GGNNCC 1 cut(s) 178
PspPI GGNCC 1 cut(s) 78
PsuI RGATCY 1 cut(s) 111
PvuI CGATCG 1 cut(s) 15
RsaI GTAC 3 cut(s) 26, 362, 413
RsaNI GTAC 3 cut(s) 25, 361, 412
RseI CAYNNNNRTG 1 cut(s) 105
SacI GAGCTC 1 cut(s) 657
Sau3AI GATC 8 cut(s) 12, 18, 111, 253, 376, 530, 580, 610
Sau96I GGNCC 1 cut(s) 78
SchI GAGTC 1 cut(s) 613
ScrFI CCNGG 1 cut(s) 151
SduI GDGCHC 2 cut(s) 449, 657
SetI ASST 7 cut(s) 32, 278, 303, 362, 452, 475, 657
SfaNI GCATC 1 cut(s) 393
SmiMI CAYNNNNRTG 1 cut(s) 105
Sse9I AATT 2 cut(s) 353, 595
SsiI CCGC 2 cut(s) 247, 282
SspMI CTAG 1 cut(s) 474
SstI GAGCTC 1 cut(s) 657
StyD4I CCNGG 1 cut(s) 149
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 1 cut(s) 173
TaiI ACGT 1 cut(s) 278
TaqI TCGA 3 cut(s) 104, 579, 607
TasI AATT 2 cut(s) 353, 595
TscAI CASTG 2 cut(s) 433, 531
TspDTI ATGAA 3 cut(s) 23, 60, 651
TspRI CASTG 2 cut(s) 433, 531
VneI GTGCAC 1 cut(s) 445
XapI RAATTY 1 cut(s) 353
XcmI CCANNNNNNNNNTGG 2 cut(s) 301, 433
XspI CTAG 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.