Rroxscaffold_7G00187340

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007718
Physical Location & Seq
Forward (+)
26751664 .. 26753825
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00187340.1

Sequence Viewer

Length: 834 bp
ATGGTAATGAGAATGGCAATGGCAGGGTCATTCTGGAAGTCATCGTGTTCGTGTTCATATACGTGTTCATTGGTTAGAGTAATAGGAGTTTCAAGAACAAGTACTGGTGCAGCATCTGCTATACTTCATGCATCATCTCTATTTCCACCTCCATCCATGGCCTTAAAAATACAAAGAAAAAGTAGCTTCACCTCCACTTGGTTGTCTCAACAGCAACCACAATTACCAGAACACAGGAACAGAAATGCCTTCTTCTTCATACGGAGCTCTAAATACGGAACCAATAGTATTAGCACTGATGGCGGAAAGGGATATCTGGAATCTACGGACCAAGAGTTGATGAGCCAGTGCGAAATGGGCACTTTCAAGACGTCAGGGCCAGGGGGTCAGCACCGCAACAAGCGAGAGTCTGCAGTACGCCTTAAGCATCTCCCAACTGGTATTATTGCACAGGCGTCTGAGGATCGATCCCAGCACATGAATCGTGCCTCAGCTTTATCTCGCCTACGCACTCTTATAGCTCTCAAAGTCAGGAACACTGTGGATCTTGATGGTTATTCCCCTCCTCGAGAGCTTCTTCAAATTCTTCCCAAATCATCTTCCATTAGAACATCAGATATTGGTAACCAAATTGGACCGAACAATCCGAAATTTGTTTATGGAATGCAAGCTCTGTTGGATCTCCTTTATGCAGTTGATGGTTCTGTCTCAGAGGCAGCAAAGTTTCTGGGGTTGAGCACAGGTGCACTGTCTCGGTTAATACTCTCAGATGATTCTCTCCGATTGGCAGTCAATGAGCTTAGGGCTTCTAAGGACATGAAGCCTCTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

277

Amino Acids

30.32

Weight (kDa)

10.01

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 115 - 174 3.4e-19 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 374
AciI CCGC 2 cut(s) 303, 394
AclWI GGATC 4 cut(s) 462, 471, 552, 687
AcsI RAATTY 2 cut(s) 582, 650
AcyI GRCGYC 2 cut(s) 371, 455
AfaI GTAC 2 cut(s) 103, 417
AfiI CCNNNNNNNGG 1 cut(s) 198
AflII CTTAAG 1 cut(s) 422
AflIII ACRYGT 1 cut(s) 62
AgsI TTSAA 3 cut(s) 93, 367, 581
AjnI CCWGG 1 cut(s) 379
AluBI AGCT 7 cut(s) 186, 267, 494, 521, 574, 671, 799
AluI AGCT 7 cut(s) 186, 267, 494, 521, 574, 671, 799
Alw21I GWGCWC 3 cut(s) 269, 740, 748
Alw26I GTCTC 3 cut(s) 210, 712, 756
Alw44I GTGCAC 1 cut(s) 744
AlwI GGATC 4 cut(s) 462, 471, 552, 687
AlwNI CAGNNNCTG 1 cut(s) 116
Ama87I CYCGRG 1 cut(s) 567
AoxI GGCC 2 cut(s) 159, 377
ApaLI GTGCAC 1 cut(s) 744
ApeKI GCWGC 2 cut(s) 110, 716
ApoI RAATTY 2 cut(s) 582, 650
AspS9I GGNCC 3 cut(s) 328, 377, 635
AsuHPI GGTGA 1 cut(s) 181
AvaI CYCGRG 1 cut(s) 567
AvaII GGWCC 2 cut(s) 328, 635
BaeGI GKGCMC 2 cut(s) 362, 748
BanII GRGCYC 1 cut(s) 269
Bbv12I GWGCWC 3 cut(s) 269, 740, 748
BbvCI CCTCAGC 1 cut(s) 490
BbvI GCAGC 2 cut(s) 122, 728
BccI CCATC 4 cut(s) 160, 293, 545, 692
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BciT130I CCWGG 1 cut(s) 381
BcoDI GTCTC 3 cut(s) 210, 712, 756
BfmI CTRYAG 1 cut(s) 411
BfrI CTTAAG 1 cut(s) 422
BisI GCNGC 2 cut(s) 111, 717
BlsI GCNGC 2 cut(s) 112, 718
BmcAI AGTACT 1 cut(s) 103
Bme1390I CCNGG 1 cut(s) 381
Bme18I GGWCC 2 cut(s) 328, 635
BmeT110I CYCGRG 1 cut(s) 567
BmgT120I GGNCC 3 cut(s) 328, 377, 635
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 1 cut(s) 381
BmsI GCATC 3 cut(s) 122, 140, 436
Bpu10I CCTNAGC 2 cut(s) 490, 800
Bsa29I ATCGAT 1 cut(s) 466
BsaAI YACGTR 1 cut(s) 63
BsaHI GRCGYC 2 cut(s) 371, 455
BsaJI CCNNGG 2 cut(s) 156, 380
Bsc4I CCNNNNNNNGG 1 cut(s) 198
Bse1I ACTGG 3 cut(s) 109, 346, 442
Bse3DI GCAATG 1 cut(s) 24
BseBI CCWGG 1 cut(s) 381
BseCI ATCGAT 1 cut(s) 466
BseDI CCNNGG 2 cut(s) 156, 380
BseGI GGATG 1 cut(s) 152
BseLI CCNNNNNNNGG 1 cut(s) 198
BseMI GCAATG 1 cut(s) 24
BseMII CTCAG 5 cut(s) 450, 504, 723, 780, 818
BseNI ACTGG 3 cut(s) 109, 346, 442
BseRI GAGGAG 1 cut(s) 555
BseSI GKGCMC 2 cut(s) 362, 748
BseXI GCAGC 2 cut(s) 122, 728
BseYI CCCAGC 1 cut(s) 471
BsgI GTGCAG 1 cut(s) 129
BshFI GGCC 2 cut(s) 161, 379
BshVI ATCGAT 1 cut(s) 466
BsiHKAI GWGCWC 3 cut(s) 269, 740, 748
BsiHKCI CYCGRG 1 cut(s) 567
BslI CCNNNNNNNGG 1 cut(s) 198
BsmAI GTCTC 3 cut(s) 210, 712, 756
BsmI GAATGC 1 cut(s) 669
BsnI GGCC 2 cut(s) 161, 379
BsoBI CYCGRG 1 cut(s) 567
Bsp1286I GDGCHC 4 cut(s) 269, 362, 740, 748
Bsp143I GATC 4 cut(s) 463, 467, 544, 679
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 2 cut(s) 303, 394
BspANI GGCC 2 cut(s) 161, 379
BspCNI CTCAG 5 cut(s) 451, 503, 722, 779, 819
BspDI ATCGAT 1 cut(s) 466
BspLI GGNNCC 1 cut(s) 280
BspMAI CTGCAG 1 cut(s) 415
BspPI GGATC 4 cut(s) 462, 471, 552, 687
BspTI CTTAAG 1 cut(s) 422
BsrDI GCAATG 1 cut(s) 24
BsrI ACTGG 3 cut(s) 109, 346, 442
BssECI CCNNGG 2 cut(s) 156, 380
BssMI GATC 4 cut(s) 463, 467, 544, 679
BssNI GRCGYC 2 cut(s) 371, 455
BssT1I CCWWGG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 2 cut(s) 541, 750
BstACI GRCGYC 2 cut(s) 371, 455
BstAFI CTTAAG 1 cut(s) 422
BstAPI GCANNNNNTGC 1 cut(s) 116
BstBAI YACGTR 1 cut(s) 63
BstC8I GCNNGC 1 cut(s) 669
BstDEI CTNAG 7 cut(s) 459, 490, 709, 766, 800, 810, 827
BstDSI CCRYGG 1 cut(s) 156
BstEII GGTNACC 1 cut(s) 623
BstF5I GGATG 1 cut(s) 152
BstKTI GATC 4 cut(s) 466, 470, 547, 682
BstMAI GTCTC 3 cut(s) 210, 712, 756
BstMBI GATC 4 cut(s) 463, 467, 544, 679
BstMWI GCNNNNNNNGC 3 cut(s) 116, 300, 357
BstNI CCWGG 1 cut(s) 381
BstPI GGTNACC 1 cut(s) 623
BstSCI CCNGG 1 cut(s) 379
BstSFI CTRYAG 1 cut(s) 411
BstSLI GKGCMC 2 cut(s) 362, 748
BstV1I GCAGC 2 cut(s) 122, 728
BstX2I RGATCY 2 cut(s) 544, 679
BstYI RGATCY 2 cut(s) 544, 679
Bsu15I ATCGAT 1 cut(s) 466
BsuRI GGCC 2 cut(s) 161, 379
BsuTUI ATCGAT 1 cut(s) 466
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 1 cut(s) 152
BtsIMutI CAGTG 4 cut(s) 294, 353, 537, 746
Cac8I GCNNGC 1 cut(s) 669
CaiI CAGNNNCTG 1 cut(s) 116
Cfr13I GGNCC 3 cut(s) 328, 377, 635
ClaI ATCGAT 1 cut(s) 466
CseI GACGC 1 cut(s) 444
Csp6I GTAC 2 cut(s) 102, 416
CviAII CATG 4 cut(s) 128, 157, 478, 817
CviQI GTAC 2 cut(s) 102, 416
DdeI CTNAG 7 cut(s) 459, 490, 709, 766, 800, 810, 827
DpnI GATC 4 cut(s) 465, 469, 546, 681
DpnII GATC 4 cut(s) 463, 467, 544, 679
EciI GGCGGA 1 cut(s) 318
Ecl136II GAGCTC 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 156
Eco24I GRGCYC 1 cut(s) 269
Eco32I GATATC 1 cut(s) 314
Eco47I GGWCC 2 cut(s) 328, 635
Eco53kI GAGCTC 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 567
Eco91I GGTNACC 1 cut(s) 623
EcoICRI GAGCTC 1 cut(s) 267
EcoO65I GGTNACC 1 cut(s) 623
EcoRII CCWGG 1 cut(s) 379
EcoRV GATATC 1 cut(s) 314
EcoT14I CCWWGG 1 cut(s) 156
EcoT22I ATGCAT 1 cut(s) 133
EcoT38I GRGCYC 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 4 cut(s) 131, 160, 481, 820
FatI CATG 4 cut(s) 127, 156, 477, 816
Fnu4HI GCNGC 2 cut(s) 111, 717
FokI GGATG 1 cut(s) 139
FriOI GRGCYC 1 cut(s) 269
Fsp4HI GCNGC 2 cut(s) 111, 717
GluI GCNGC 2 cut(s) 111, 717
GsaI CCCAGC 1 cut(s) 475
HaeIII GGCC 2 cut(s) 161, 379
HgaI GACGC 1 cut(s) 444
Hin1I GRCGYC 2 cut(s) 371, 455
Hin1II CATG 4 cut(s) 131, 160, 481, 820
HinfI GANTC 4 cut(s) 320, 407, 481, 773
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 746
Hpy188I TCNGA 7 cut(s) 460, 616, 648, 712, 769, 782, 828
Hpy188III TCNNGA 7 cut(s) 34, 93, 317, 367, 532, 548, 569
Hpy8I GTNNAC 1 cut(s) 746
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 2 cut(s) 541, 750
HpyCH4IV ACGT 2 cut(s) 62, 371
HpyCH4V TGCA 7 cut(s) 110, 131, 413, 449, 667, 692, 746
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 300, 357
HpyF3I CTNAG 7 cut(s) 459, 490, 709, 766, 800, 810, 827
HpySE526I ACGT 2 cut(s) 62, 371
Hsp92I GRCGYC 2 cut(s) 371, 455
Hsp92II CATG 4 cut(s) 131, 160, 481, 820
Kzo9I GATC 4 cut(s) 463, 467, 544, 679
LmnI GCTCC 1 cut(s) 264
Lsp1109I GCAGC 2 cut(s) 122, 728
LweI GCATC 3 cut(s) 122, 140, 436
MaeII ACGT 2 cut(s) 62, 371
MaeIII GTNAC 1 cut(s) 623
MalI GATC 4 cut(s) 465, 469, 546, 681
MboI GATC 4 cut(s) 463, 467, 544, 679
MboII GAAGA 5 cut(s) 244, 247, 569, 578, 591
MflI RGATCY 2 cut(s) 544, 679
MhlI GDGCHC 4 cut(s) 269, 362, 740, 748
MluCI AATT 4 cut(s) 221, 582, 630, 650
MlyI GAGTC 1 cut(s) 416
MmeI TCCRAC 1 cut(s) 657
MnlI CCTC 9 cut(s) 159, 202, 454, 499, 573, 576, 706, 822, 834
Mph1103I ATGCAT 1 cut(s) 133
MseI TTAA 3 cut(s) 164, 423, 758
MslI CAYNNNNRTG 1 cut(s) 61
MspCI CTTAAG 1 cut(s) 422
MspR9I CCNGG 1 cut(s) 381
Mva1269I GAATGC 1 cut(s) 669
MvaI CCWGG 1 cut(s) 381
MwoI GCNNNNNNNGC 3 cut(s) 116, 300, 357
NcoI CCATGG 1 cut(s) 156
NdeII GATC 4 cut(s) 463, 467, 544, 679
NlaIII CATG 4 cut(s) 131, 160, 481, 820
NlaIV GGNNCC 1 cut(s) 280
NsiI ATGCAT 1 cut(s) 133
PaeR7I CTCGAG 1 cut(s) 567
PctI GAATGC 1 cut(s) 669
PfeI GAWTC 3 cut(s) 320, 481, 773
PkrI GCNGC 2 cut(s) 112, 718
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
Ppu21I YACGTR 1 cut(s) 63
Psp124BI GAGCTC 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 379
PspEI GGTNACC 1 cut(s) 623
PspFI CCCAGC 1 cut(s) 471
PspGI CCWGG 1 cut(s) 379
PspN4I GGNNCC 1 cut(s) 280
PspPI GGNCC 3 cut(s) 328, 377, 635
PstI CTGCAG 1 cut(s) 415
PstNI CAGNNNCTG 1 cut(s) 116
PsuI RGATCY 2 cut(s) 544, 679
RsaI GTAC 2 cut(s) 103, 417
RsaNI GTAC 2 cut(s) 102, 416
RseI CAYNNNNRTG 1 cut(s) 61
SacI GAGCTC 1 cut(s) 269
SaqAI TTAA 3 cut(s) 164, 423, 758
SatI GCNGC 2 cut(s) 111, 717
Sau3AI GATC 4 cut(s) 463, 467, 544, 679
Sau96I GGNCC 3 cut(s) 328, 377, 635
ScaI AGTACT 1 cut(s) 103
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 1 cut(s) 381
SduI GDGCHC 4 cut(s) 269, 362, 740, 748
SfaNI GCATC 3 cut(s) 122, 140, 436
SfcI CTRYAG 1 cut(s) 411
Sfr274I CTCGAG 1 cut(s) 567
SinI GGWCC 2 cut(s) 328, 635
SlaI CTCGAG 1 cut(s) 567
SmiMI CAYNNNNRTG 1 cut(s) 61
SmlI CTYRAG 2 cut(s) 422, 567
SmoI CTYRAG 2 cut(s) 422, 567
Sse9I AATT 4 cut(s) 221, 582, 630, 650
SsiI CCGC 2 cut(s) 303, 394
SstI GAGCTC 1 cut(s) 269
StyD4I CCNGG 1 cut(s) 379
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 2 cut(s) 541, 750
TaiI ACGT 2 cut(s) 65, 374
TaqI TCGA 2 cut(s) 466, 568
TaqII GACCGA 1 cut(s) 652
TasI AATT 4 cut(s) 221, 582, 630, 650
TatI WGTACW 1 cut(s) 101
TfiI GAWTC 3 cut(s) 320, 481, 773
Tru1I TTAA 3 cut(s) 164, 423, 758
Tru9I TTAA 3 cut(s) 164, 423, 758
TscAI CASTG 4 cut(s) 301, 353, 544, 753
TseI GCWGC 2 cut(s) 110, 716
TspDTI ATGAA 6 cut(s) 45, 57, 116, 247, 494, 833
TspGWI ACGGA 3 cut(s) 277, 291, 341
TspRI CASTG 4 cut(s) 301, 353, 544, 753
Vha464I CTTAAG 1 cut(s) 422
VneI GTGCAC 1 cut(s) 744
VpaK11BI GGWCC 2 cut(s) 328, 635
XapI RAATTY 2 cut(s) 582, 650
XhoI CTCGAG 1 cut(s) 567
ZraI GACGTC 1 cut(s) 372
ZrmI AGTACT 1 cut(s) 103
Zsp2I ATGCAT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.