Rorug06G0138500

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007718
Physical Location & Seq
Reverse (-)
19594155 .. 19594664
510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0138500.1

Sequence Viewer

Length: 510 bp
ATGGCTGACATGGAGGCTGGCAATCCAGCAAGCCTTCAACTATCTGACTCTGCGCGTGTATCATTGGGTACGGCGGGGCTTGTGGCGCTGCAAGTTGATCGGTGGTACATAGTGGGTAGGCTGCTTGGCCCCAAAGCCAAGTACCCGGGATTCAAAGGTACAATCTCTACTATTTGGAAAAGCAAATATGGGTTGACTATCCAAGACATGGGTGATCATTTCATTTTTCAATTTGAACGCGAGGCTGAGCGTAACAAGATTTTGCCGTGGTTTTATAGGAATACTATGCTATTACTTGATGCATATGATGGCATTGGGTCAGCGGCAAAGGTTCCATTGAATCTGTTGGAGACATGGGTAGCTGTCAAAAGTTTGCCCGTGTGTCTGAGAAACAAGACAGCTTTGAGTTTTGTTGCTTCGACTCTAGGCAGGCTGACCAGATTCGACCAAACGACTTTGTATAGGAAGGAAGAAGAACAACGGATCAGGCTGGTGTTTGATGTTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

169

Amino Acids

19.13

Weight (kDa)

9.55

Isoelectric Point (pI)

31.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 34 - 102 7.5e-10 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 208
AccII CGCG 2 cut(s) 55, 240
AciI CCGC 2 cut(s) 74, 323
AclWI GGATC 1 cut(s) 491
AfaI GTAC 4 cut(s) 70, 107, 143, 160
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 5 cut(s) 38, 154, 230, 236, 340
AluBI AGCT 2 cut(s) 362, 401
AluI AGCT 2 cut(s) 362, 401
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 1 cut(s) 491
Ama87I CYCGRG 1 cut(s) 145
AoxI GGCC 1 cut(s) 127
ApeKI GCWGC 2 cut(s) 88, 121
AspLEI GCGC 2 cut(s) 55, 88
AspS9I GGNCC 1 cut(s) 128
AsuC2I CCSGG 2 cut(s) 146, 147
AsuHPI GGTGA 1 cut(s) 224
AvaI CYCGRG 1 cut(s) 145
BbvI GCAGC 2 cut(s) 75, 108
BccI CCATC 1 cut(s) 302
BceAI ACGGC 2 cut(s) 87, 250
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 2 cut(s) 146, 147
BcoDI GTCTC 1 cut(s) 344
BfaI CTAG 1 cut(s) 425
BfoI RGCGCY 1 cut(s) 89
BisI GCNGC 3 cut(s) 89, 122, 324
BlpI GCTNAGC 1 cut(s) 246
BlsI GCNGC 3 cut(s) 90, 123, 325
Bme1390I CCNGG 2 cut(s) 146, 147
BmeT110I CYCGRG 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 2 cut(s) 130, 333
BmrFI CCNGG 2 cut(s) 146, 147
BmsI GCATC 1 cut(s) 289
Bpu1102I GCTNAGC 1 cut(s) 246
BpuMI CCSGG 2 cut(s) 146, 147
BsaJI CCNNGG 2 cut(s) 145, 266
Bsc4I CCNNNNNNNGG 1 cut(s) 208
BseDI CCNNGG 2 cut(s) 145, 266
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 2 cut(s) 237, 377
BseXI GCAGC 2 cut(s) 75, 108
Bsh1236I CGCG 2 cut(s) 55, 240
BshFI GGCC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 1 cut(s) 344
BsnI GGCC 1 cut(s) 129
BsoBI CYCGRG 1 cut(s) 145
Bsp143I GATC 3 cut(s) 97, 214, 483
Bsp1720I GCTNAGC 1 cut(s) 246
BspACI CCGC 2 cut(s) 74, 323
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 2 cut(s) 238, 378
BspFNI CGCG 2 cut(s) 55, 240
BspLI GGNNCC 2 cut(s) 130, 333
BspPI GGATC 1 cut(s) 491
BssECI CCNNGG 2 cut(s) 145, 266
BssMI GATC 3 cut(s) 97, 214, 483
BstC8I GCNNGC 3 cut(s) 19, 31, 431
BstDEI CTNAG 2 cut(s) 246, 386
BstDSI CCRYGG 1 cut(s) 266
BstFNI CGCG 2 cut(s) 55, 240
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 2 cut(s) 55, 88
BstKTI GATC 3 cut(s) 100, 217, 486
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 3 cut(s) 97, 214, 483
BstMWI GCNNNNNNNGC 1 cut(s) 85
BstSCI CCNGG 2 cut(s) 144, 145
BstUI CGCG 2 cut(s) 55, 240
BstV1I GCAGC 2 cut(s) 75, 108
BsuRI GGCC 1 cut(s) 129
BtgI CCRYGG 1 cut(s) 266
Cac8I GCNNGC 3 cut(s) 19, 31, 431
CfoI GCGC 2 cut(s) 55, 88
Cfr13I GGNCC 1 cut(s) 128
Cfr9I CCCGGG 1 cut(s) 145
Csp6I GTAC 4 cut(s) 69, 106, 142, 159
CviAII CATG 3 cut(s) 10, 208, 354
CviQI GTAC 4 cut(s) 69, 106, 142, 159
DdeI CTNAG 2 cut(s) 246, 386
DpnI GATC 3 cut(s) 99, 216, 485
DpnII GATC 3 cut(s) 97, 214, 483
Eco88I CYCGRG 1 cut(s) 145
EcoT22I ATGCAT 1 cut(s) 304
FaeI CATG 3 cut(s) 13, 211, 357
FatI CATG 3 cut(s) 9, 207, 353
FauI CCCGC 1 cut(s) 67
FauNDI CATATG 1 cut(s) 304
FbaI TGATCA 1 cut(s) 214
Fnu4HI GCNGC 3 cut(s) 89, 122, 324
Fsp4HI GCNGC 3 cut(s) 89, 122, 324
FspBI CTAG 1 cut(s) 425
GlaI GCGC 2 cut(s) 54, 87
GluI GCNGC 3 cut(s) 89, 122, 324
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 1 cut(s) 129
HapII CCGG 1 cut(s) 146
HhaI GCGC 2 cut(s) 55, 88
Hin1II CATG 3 cut(s) 13, 211, 357
Hin6I GCGC 2 cut(s) 53, 86
HinP1I GCGC 2 cut(s) 53, 86
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HinfI GANTC 5 cut(s) 47, 150, 340, 421, 441
HpaII CCGG 1 cut(s) 146
HphI GGTGA 1 cut(s) 224
Hpy166II GTNNAC 1 cut(s) 195
Hpy188I TCNGA 2 cut(s) 46, 387
Hpy8I GTNNAC 1 cut(s) 195
HpyAV CCTTC 2 cut(s) 44, 460
HpyCH4V TGCA 2 cut(s) 91, 302
HpyF10VI GCNNNNNNNGC 1 cut(s) 85
HpyF3I CTNAG 2 cut(s) 246, 386
Hsp92II CATG 3 cut(s) 13, 211, 357
HspAI GCGC 2 cut(s) 53, 86
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 3 cut(s) 97, 214, 483
LpnPI CCDG 7 cut(s) 3, 39, 159, 415, 451, 472, 476
Lsp1109I GCAGC 2 cut(s) 75, 108
LweI GCATC 1 cut(s) 289
MaeI CTAG 1 cut(s) 425
MaeIII GTNAC 1 cut(s) 251
MalI GATC 3 cut(s) 99, 216, 485
MboI GATC 3 cut(s) 97, 214, 483
MboII GAAGA 2 cut(s) 482, 485
MluCI AATT 1 cut(s) 230
MlyI GAGTC 2 cut(s) 41, 415
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 2 cut(s) 7, 235
Mph1103I ATGCAT 1 cut(s) 304
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 2 cut(s) 146, 147
MvnI CGCG 2 cut(s) 55, 240
MwoI GCNNNNNNNGC 1 cut(s) 85
NciI CCSGG 2 cut(s) 146, 147
NdeI CATATG 1 cut(s) 304
NdeII GATC 3 cut(s) 97, 214, 483
NlaIII CATG 3 cut(s) 13, 211, 357
NlaIV GGNNCC 2 cut(s) 130, 333
NsiI ATGCAT 1 cut(s) 304
PfeI GAWTC 3 cut(s) 150, 340, 441
PflMI CCANNNNNTGG 1 cut(s) 208
PkrI GCNGC 3 cut(s) 90, 123, 325
PleI GAGTC 2 cut(s) 41, 415
PpsI GAGTC 2 cut(s) 41, 415
PspN4I GGNNCC 2 cut(s) 130, 333
PspPI GGNCC 1 cut(s) 128
RsaI GTAC 4 cut(s) 70, 107, 143, 160
RsaNI GTAC 4 cut(s) 69, 106, 142, 159
SatI GCNGC 3 cut(s) 89, 122, 324
Sau3AI GATC 3 cut(s) 97, 214, 483
Sau96I GGNCC 1 cut(s) 128
SchI GAGTC 2 cut(s) 41, 415
ScrFI CCNGG 2 cut(s) 146, 147
SetI ASST 4 cut(s) 160, 333, 364, 403
SfaNI GCATC 1 cut(s) 289
SmaI CCCGGG 1 cut(s) 147
Sse9I AATT 1 cut(s) 230
SsiI CCGC 2 cut(s) 74, 323
SspMI CTAG 1 cut(s) 425
StyD4I CCNGG 2 cut(s) 144, 145
TaqI TCGA 2 cut(s) 419, 444
TasI AATT 1 cut(s) 230
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 3 cut(s) 150, 340, 441
TseI GCWGC 2 cut(s) 88, 121
TspDTI ATGAA 1 cut(s) 211
TspGWI ACGGA 1 cut(s) 496
TspMI CCCGGG 1 cut(s) 145
Van91I CCANNNNNTGG 1 cut(s) 208
XmaI CCCGGG 1 cut(s) 145
XspI CTAG 1 cut(s) 425
Zsp2I ATGCAT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.