Rw6G021750

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0007718
Physical Location & Seq
Reverse (-)
43307047 .. 43307874
828 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G021750.1

Sequence Viewer

Length: 828 bp
ATGAGAATGACAATGGCAGGGTCATTCTGGAAGTCATCGTGTTCGTGTTCATATACGTGTTCATTGGTTAGAGTAATAGGAGTTTCAAGAACAAGTAGTGGTGCAGCAGCTGCTATACTTCAAGCATCATCTCTATTTCCACCTCCATCCATGGCCTTAAAAATACAAAGAAAAAGTAGCTTAACCAAGTGGAGGTGGTCTCAACAGCAACCACAATTACCAGAAGACAGGAAGAGCAATGCCTTCTTCTTCATACGGAGCTCTAAATACGGAAACAATAGTATTAGCACTGATGGTGGAAAGGGATATCTGGAATCTACGGACCAAGAGTTGATGAGCCAGTGCGAAATGGGCACTTTCAAGACGTCAGGGCCGGGGGGTCAGCATCGCAACAAGCGAGAGTCTGCAGTACGCCTTAAGCATCTCCCAACTGGTATTATTGCACAGGCTTCTGAGGATCGATCCCAGCACATGAATCGTGCCTCAGCTTTGTCTCGCCTACGCACTCTTATAGCTCTCAAAGTCAGGAACACTGTGGATCTCGATGGTTATTCCCCTCCTCAAGAGCTTCTTCAAATTCTTCCCAAATCATCTTCCATTAGAACATCAGATATTGGTAACCAAATTGGACCGAACAATCCGAAATTTGTCTACGGAATGCAAGCTCTGTTGGATCTCCTTTATGCAGTTGATGGTTCTGTCTCAGAGGCAGCAAAGTTTCTGGGGTTGAGCACAGGTGCACTGTCTCGATTAATACTCTCTGATGATTCTCTCCGATTGGCAGTCAATGAGCTTAGGGCTTCTAAGGACATGAAGCCTTTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

275

Amino Acids

30.13

Weight (kDa)

9.94

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 113 - 172 3.3e-19 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 368
AccI GTMKAC 1 cut(s) 651
AclWI GGATC 4 cut(s) 456, 465, 546, 681
AcsI RAATTY 2 cut(s) 576, 644
AcyI GRCGYC 1 cut(s) 365
AfaI GTAC 1 cut(s) 411
AfiI CCNNNNNNNGG 1 cut(s) 192
AflII CTTAAG 1 cut(s) 416
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 4 cut(s) 87, 122, 361, 575
AluBI AGCT 8 cut(s) 110, 180, 261, 488, 515, 568, 665, 793
AluI AGCT 8 cut(s) 110, 180, 261, 488, 515, 568, 665, 793
Alw21I GWGCWC 3 cut(s) 263, 734, 742
Alw26I GTCTC 4 cut(s) 204, 498, 706, 750
Alw44I GTGCAC 1 cut(s) 738
AlwI GGATC 4 cut(s) 456, 465, 546, 681
AlwNI CAGNNNCTG 1 cut(s) 110
AoxI GGCC 2 cut(s) 153, 371
ApaLI GTGCAC 1 cut(s) 738
ApeKI GCWGC 4 cut(s) 104, 107, 110, 710
ApoI RAATTY 2 cut(s) 576, 644
AseI ATTAAT 1 cut(s) 752
AspS9I GGNCC 3 cut(s) 322, 371, 629
AsuC2I CCSGG 1 cut(s) 375
AvaII GGWCC 2 cut(s) 322, 629
BaeGI GKGCMC 2 cut(s) 356, 742
BanII GRGCYC 1 cut(s) 263
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 3 cut(s) 263, 734, 742
BbvCI CCTCAGC 1 cut(s) 484
BbvI GCAGC 4 cut(s) 97, 116, 119, 722
BccI CCATC 4 cut(s) 154, 287, 539, 686
BcgI CGANNNNNNTGC 2 cut(s) 458, 492
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 4 cut(s) 204, 498, 706, 750
BfmI CTRYAG 1 cut(s) 405
BfrI CTTAAG 1 cut(s) 416
BisI GCNGC 4 cut(s) 105, 108, 111, 711
BlsI GCNGC 4 cut(s) 106, 109, 112, 712
Bme1390I CCNGG 1 cut(s) 375
Bme18I GGWCC 2 cut(s) 322, 629
BmgT120I GGNCC 3 cut(s) 322, 371, 629
BmrFI CCNGG 1 cut(s) 375
BmsI GCATC 3 cut(s) 134, 394, 430
BpiI GAAGAC 1 cut(s) 231
BplI GAGNNNNNCTC 2 cut(s) 184, 216
Bpu10I CCTNAGC 2 cut(s) 484, 794
BpuEI CTTGAG 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 375
Bsa29I ATCGAT 1 cut(s) 460
BsaAI YACGTR 1 cut(s) 57
BsaHI GRCGYC 1 cut(s) 365
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 2 cut(s) 150, 374
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse1I ACTGG 2 cut(s) 340, 436
Bse3DI GCAATG 1 cut(s) 244
BseCI ATCGAT 1 cut(s) 460
BseDI CCNNGG 2 cut(s) 150, 374
BseGI GGATG 1 cut(s) 146
BseLI CCNNNNNNNGG 1 cut(s) 192
BseMI GCAATG 1 cut(s) 244
BseMII CTCAG 3 cut(s) 444, 498, 717
BseNI ACTGG 2 cut(s) 340, 436
BseRI GAGGAG 1 cut(s) 549
BseSI GKGCMC 2 cut(s) 356, 742
BseXI GCAGC 4 cut(s) 97, 116, 119, 722
BseYI CCCAGC 1 cut(s) 465
BsgI GTGCAG 1 cut(s) 123
BshFI GGCC 2 cut(s) 155, 373
BshVI ATCGAT 1 cut(s) 460
BsiHKAI GWGCWC 3 cut(s) 263, 734, 742
BsiSI CCGG 1 cut(s) 374
BslI CCNNNNNNNGG 1 cut(s) 192
BsmAI GTCTC 4 cut(s) 204, 498, 706, 750
BsmI GAATGC 1 cut(s) 663
BsnI GGCC 2 cut(s) 155, 373
Bso31I GGTCTC 1 cut(s) 204
Bsp1286I GDGCHC 4 cut(s) 263, 356, 734, 742
Bsp143I GATC 4 cut(s) 457, 461, 538, 673
Bsp19I CCATGG 1 cut(s) 150
BspANI GGCC 2 cut(s) 155, 373
BspCNI CTCAG 3 cut(s) 445, 497, 716
BspDI ATCGAT 1 cut(s) 460
BspMAI CTGCAG 1 cut(s) 409
BspPI GGATC 4 cut(s) 456, 465, 546, 681
BspQI GCTCTTC 1 cut(s) 227
BspTI CTTAAG 1 cut(s) 416
BspTNI GGTCTC 1 cut(s) 204
BsrDI GCAATG 1 cut(s) 244
BsrI ACTGG 2 cut(s) 340, 436
BssECI CCNNGG 2 cut(s) 150, 374
BssMI GATC 4 cut(s) 457, 461, 538, 673
BssNI GRCGYC 1 cut(s) 365
BssT1I CCWWGG 1 cut(s) 150
Bst4CI ACNGT 2 cut(s) 535, 744
Bst6I CTCTTC 1 cut(s) 227
BstACI GRCGYC 1 cut(s) 365
BstAFI CTTAAG 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 110
BstBAI YACGTR 1 cut(s) 57
BstC8I GCNNGC 1 cut(s) 663
BstDEI CTNAG 5 cut(s) 453, 484, 703, 794, 804
BstDSI CCRYGG 1 cut(s) 150
BstEII GGTNACC 1 cut(s) 617
BstF5I GGATG 1 cut(s) 146
BstKTI GATC 4 cut(s) 460, 464, 541, 676
BstMAI GTCTC 4 cut(s) 204, 498, 706, 750
BstMBI GATC 4 cut(s) 457, 461, 538, 673
BstMWI GCNNNNNNNGC 2 cut(s) 110, 351
BstPI GGTNACC 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 373
BstSFI CTRYAG 1 cut(s) 405
BstSLI GKGCMC 2 cut(s) 356, 742
BstV1I GCAGC 4 cut(s) 97, 116, 119, 722
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 2 cut(s) 538, 673
BstYI RGATCY 2 cut(s) 538, 673
Bsu15I ATCGAT 1 cut(s) 460
BsuRI GGCC 2 cut(s) 155, 373
BsuTUI ATCGAT 1 cut(s) 460
BtgI CCRYGG 1 cut(s) 150
BtgZI GCGATG 1 cut(s) 371
BtsCI GGATG 1 cut(s) 146
BtsIMutI CAGTG 4 cut(s) 288, 347, 531, 740
Cac8I GCNNGC 1 cut(s) 663
CaiI CAGNNNCTG 1 cut(s) 110
Cfr13I GGNCC 3 cut(s) 322, 371, 629
ClaI ATCGAT 1 cut(s) 460
Csp6I GTAC 1 cut(s) 410
CviAII CATG 3 cut(s) 151, 472, 811
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 5 cut(s) 453, 484, 703, 794, 804
DpnI GATC 4 cut(s) 459, 463, 540, 675
DpnII GATC 4 cut(s) 457, 461, 538, 673
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Ecl136II GAGCTC 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 263
Eco31I GGTCTC 1 cut(s) 204
Eco32I GATATC 1 cut(s) 308
Eco47I GGWCC 2 cut(s) 322, 629
Eco53kI GAGCTC 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 617
EcoICRI GAGCTC 1 cut(s) 261
EcoO65I GGTNACC 1 cut(s) 617
EcoRV GATATC 1 cut(s) 308
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 3 cut(s) 154, 475, 814
FaiI YATR 9 cut(s) 52, 54, 116, 152, 254, 473, 512, 684, 812
FalI AAGNNNNNCTT 2 cut(s) 555, 587
FatI CATG 3 cut(s) 150, 471, 810
FblI GTMKAC 1 cut(s) 651
Fnu4HI GCNGC 4 cut(s) 105, 108, 111, 711
FokI GGATG 1 cut(s) 133
FriOI GRGCYC 1 cut(s) 263
Fsp4HI GCNGC 4 cut(s) 105, 108, 111, 711
GluI GCNGC 4 cut(s) 105, 108, 111, 711
GsaI CCCAGC 1 cut(s) 469
HaeIII GGCC 2 cut(s) 155, 373
HapII CCGG 1 cut(s) 374
Hin1I GRCGYC 1 cut(s) 365
Hin1II CATG 3 cut(s) 154, 475, 814
HinfI GANTC 4 cut(s) 314, 401, 475, 767
HpaII CCGG 1 cut(s) 374
Hpy166II GTNNAC 2 cut(s) 652, 740
Hpy188I TCNGA 6 cut(s) 454, 610, 642, 706, 763, 776
Hpy188III TCNNGA 8 cut(s) 28, 87, 311, 361, 526, 542, 563, 747
Hpy8I GTNNAC 2 cut(s) 652, 740
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 2 cut(s) 535, 744
HpyCH4IV ACGT 2 cut(s) 56, 365
HpyCH4V TGCA 6 cut(s) 104, 407, 443, 661, 686, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 351
HpyF3I CTNAG 5 cut(s) 453, 484, 703, 794, 804
HpySE526I ACGT 2 cut(s) 56, 365
Hsp92I GRCGYC 1 cut(s) 365
Hsp92II CATG 3 cut(s) 154, 475, 814
Kzo9I GATC 4 cut(s) 457, 461, 538, 673
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 1 cut(s) 258
Lsp1109I GCAGC 4 cut(s) 97, 116, 119, 722
LweI GCATC 3 cut(s) 134, 394, 430
MaeII ACGT 2 cut(s) 56, 365
MaeIII GTNAC 1 cut(s) 617
MalI GATC 4 cut(s) 459, 463, 540, 675
MboI GATC 4 cut(s) 457, 461, 538, 673
MboII GAAGA 7 cut(s) 236, 238, 241, 244, 563, 572, 585
MflI RGATCY 2 cut(s) 538, 673
MhlI GDGCHC 4 cut(s) 263, 356, 734, 742
MluCI AATT 4 cut(s) 215, 576, 624, 644
MlyI GAGTC 1 cut(s) 410
MmeI TCCRAC 1 cut(s) 651
MnlI CCTC 8 cut(s) 153, 186, 448, 493, 567, 570, 700, 816
MseI TTAA 4 cut(s) 158, 182, 417, 752
MslI CAYNNNNRTG 1 cut(s) 55
MspA1I CMGCKG 1 cut(s) 110
MspCI CTTAAG 1 cut(s) 416
MspI CCGG 1 cut(s) 374
MspR9I CCNGG 1 cut(s) 375
Mva1269I GAATGC 1 cut(s) 663
MwoI GCNNNNNNNGC 2 cut(s) 110, 351
NciI CCSGG 1 cut(s) 375
NcoI CCATGG 1 cut(s) 150
NdeII GATC 4 cut(s) 457, 461, 538, 673
NlaIII CATG 3 cut(s) 154, 475, 814
PciSI GCTCTTC 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 394
PctI GAATGC 1 cut(s) 663
PfeI GAWTC 3 cut(s) 314, 475, 767
PkrI GCNGC 4 cut(s) 106, 109, 112, 712
PleI GAGTC 1 cut(s) 409
PpsI GAGTC 1 cut(s) 409
Ppu21I YACGTR 1 cut(s) 57
PshBI ATTAAT 1 cut(s) 752
Psp124BI GAGCTC 1 cut(s) 263
PspEI GGTNACC 1 cut(s) 617
PspFI CCCAGC 1 cut(s) 465
PspPI GGNCC 3 cut(s) 322, 371, 629
PstI CTGCAG 1 cut(s) 409
PstNI CAGNNNCTG 1 cut(s) 110
PsuI RGATCY 2 cut(s) 538, 673
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
RseI CAYNNNNRTG 1 cut(s) 55
SacI GAGCTC 1 cut(s) 263
SapI GCTCTTC 1 cut(s) 227
SaqAI TTAA 4 cut(s) 158, 182, 417, 752
SatI GCNGC 4 cut(s) 105, 108, 111, 711
Sau3AI GATC 4 cut(s) 457, 461, 538, 673
Sau96I GGNCC 3 cut(s) 322, 371, 629
SchI GAGTC 1 cut(s) 410
ScrFI CCNGG 1 cut(s) 375
SduI GDGCHC 4 cut(s) 263, 356, 734, 742
SfaNI GCATC 3 cut(s) 134, 394, 430
SfcI CTRYAG 1 cut(s) 405
SinI GGWCC 2 cut(s) 322, 629
SmiMI CAYNNNNRTG 1 cut(s) 55
SmlI CTYRAG 2 cut(s) 416, 561
SmoI CTYRAG 2 cut(s) 416, 561
Sse9I AATT 4 cut(s) 215, 576, 624, 644
SstI GAGCTC 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 373
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 535, 744
TaiI ACGT 2 cut(s) 59, 368
TaqI TCGA 3 cut(s) 460, 543, 748
TaqII GACCGA 1 cut(s) 646
TasI AATT 4 cut(s) 215, 576, 624, 644
TfiI GAWTC 3 cut(s) 314, 475, 767
Tru1I TTAA 4 cut(s) 158, 182, 417, 752
Tru9I TTAA 4 cut(s) 158, 182, 417, 752
TscAI CASTG 4 cut(s) 295, 347, 538, 747
TseI GCWGC 4 cut(s) 104, 107, 110, 710
TspDTI ATGAA 5 cut(s) 39, 51, 241, 488, 827
TspGWI ACGGA 4 cut(s) 271, 285, 335, 669
TspRI CASTG 4 cut(s) 295, 347, 538, 747
Vha464I CTTAAG 1 cut(s) 416
VneI GTGCAC 1 cut(s) 738
VpaK11BI GGWCC 2 cut(s) 322, 629
VspI ATTAAT 1 cut(s) 752
XapI RAATTY 2 cut(s) 576, 644
XmiI GTMKAC 1 cut(s) 651
ZraI GACGTC 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.