Rorug06G0138600

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007718
Physical Location & Seq
Forward (+)
19607248 .. 19614187
6940 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0138600.1

Sequence Viewer

Length: 591 bp
ATGGCGAAACCAAAAGCTCCGAGACAAACCCTCAACTCCTACACTGTCAAACCCATCAACAAGACCGTCAGAGCCGGCGACTGCGTTCTGATGCGGCCGTCGGAGCCGGGGAAGCCGTCCTACGTGGCGAAGATCGAGCGGATCGAGGCGGACAGCCGCGGAGCCAACGTCAAGGTGCACGTGCGGTGGTACTACCGGCCGGAGGAGTCGATCGGCGGCCGGAGACAGTTCCATGGATCCAAGGAGGTTTTTCTCTCCGACCACCACGACGTGCAGAGCGCCGACACCATAGAGGGGAAGTGTACGGTGCACACGTTCAGGGGGTATAGCAAGCTCGACGCGGTTGGAAACGACGACTTCTTTTGTCGTTTTGAGTATAATTCCACTACTGGTTCCTTCAATCCTGACCGAGTCGCCGTGTACTGCAAGTGTGAGATGCCTTACAACCCTGATGACCTCATGGTTCAGTGTGAAGGTTGTAGTGATTGGTTGTTCTCCCGAGGGACAAAAGAAGTTGGAGAATTCGCATACTGTCTCCAGACAGTTGGATACAAAGGTGGAGACAAAACGTCGCCGGAGGTGATATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

196

Amino Acids

22.11

Weight (kDa)

7.55

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAH PF01426 22 - 136 1.2e-24 BAH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 139
AccII CGCG 2 cut(s) 159, 341
AciI CCGC 8 cut(s) 94, 139, 149, 157, 159, 184, 216, 341
AclWI GGATC 3 cut(s) 149, 231, 244
AcoI YGGCCR 3 cut(s) 95, 197, 217
AcsI RAATTY 1 cut(s) 521
AcvI CACGTG 1 cut(s) 181
AdeI CACNNNGTG 1 cut(s) 271
AfaI GTAC 3 cut(s) 191, 304, 422
AfiI CCNNNNNNNGG 2 cut(s) 202, 294
AflIII ACRYGT 1 cut(s) 312
AgsI TTSAA 1 cut(s) 400
AhdI GACNNNNNGTC 1 cut(s) 568
AjiI CACGTC 1 cut(s) 271
AluBI AGCT 2 cut(s) 17, 334
AluI AGCT 2 cut(s) 17, 334
Alw21I GWGCWC 2 cut(s) 180, 312
Alw26I GTCTC 4 cut(s) 16, 217, 539, 555
Alw44I GTGCAC 2 cut(s) 176, 308
AlwI GGATC 3 cut(s) 149, 231, 244
Ama87I CYCGRG 1 cut(s) 498
AoxI GGCC 3 cut(s) 95, 197, 217
ApaLI GTGCAC 2 cut(s) 176, 308
ApoI RAATTY 1 cut(s) 521
AspLEI GCGC 1 cut(s) 281
AsuC2I CCSGG 1 cut(s) 108
AvaI CYCGRG 1 cut(s) 498
BaeGI GKGCMC 2 cut(s) 180, 312
BaeI ACNNNNGTAYC 2 cut(s) 541, 574
BamHI GGATCC 1 cut(s) 236
BarI GAAGNNNNNNTAC 2 cut(s) 104, 136
BbrPI CACGTG 1 cut(s) 181
Bbv12I GWGCWC 2 cut(s) 180, 312
BccI CCATC 1 cut(s) 62
BceAI ACGGC 3 cut(s) 82, 100, 401
BciVI GTATCC 1 cut(s) 542
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 4 cut(s) 16, 217, 539, 555
BfoI RGCGCY 1 cut(s) 282
BfuI GTATCC 1 cut(s) 542
BisI GCNGC 3 cut(s) 95, 157, 217
BlsI GCNGC 3 cut(s) 96, 158, 218
Bme1390I CCNGG 1 cut(s) 108
BmeRI GACNNNNNGTC 1 cut(s) 568
BmeT110I CYCGRG 1 cut(s) 498
BmgBI CACGTC 1 cut(s) 271
BmiI GGNNCC 4 cut(s) 105, 163, 238, 394
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 2 cut(s) 81, 426
BpmI CTGGAG 1 cut(s) 521
BpuMI CCSGG 1 cut(s) 108
BsaAI YACGTR 2 cut(s) 124, 181
BsaJI CCNNGG 5 cut(s) 107, 157, 232, 240, 499
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 294
Bse118I RCCGGY 2 cut(s) 74, 195
Bse1I ACTGG 1 cut(s) 394
BseDI CCNNGG 5 cut(s) 107, 157, 232, 240, 499
BseLI CCNNNNNNNGG 2 cut(s) 202, 294
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 218
BseSI GKGCMC 2 cut(s) 180, 312
BseX3I CGGCCG 3 cut(s) 95, 197, 217
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 2 cut(s) 159, 341
Bsh1285I CGRYCG 4 cut(s) 98, 200, 213, 220
BshFI GGCC 3 cut(s) 97, 199, 219
BsiEI CGRYCG 4 cut(s) 98, 200, 213, 220
BsiHKAI GWGCWC 2 cut(s) 180, 312
BsiHKCI CYCGRG 1 cut(s) 498
BsiSI CCGG 6 cut(s) 75, 107, 196, 200, 220, 575
BslFI GGGAC 1 cut(s) 517
BslI CCNNNNNNNGG 2 cut(s) 202, 294
BsmAI GTCTC 4 cut(s) 16, 217, 539, 555
BsmFI GGGAC 1 cut(s) 517
BsnI GGCC 3 cut(s) 97, 199, 219
BsoBI CYCGRG 1 cut(s) 498
Bsp1286I GDGCHC 2 cut(s) 180, 312
Bsp143I GATC 4 cut(s) 132, 141, 210, 236
Bsp19I CCATGG 1 cut(s) 232
BspACI CCGC 8 cut(s) 94, 139, 149, 157, 159, 184, 216, 341
BspANI GGCC 3 cut(s) 97, 199, 219
BspFNI CGCG 2 cut(s) 159, 341
BspLI GGNNCC 4 cut(s) 105, 163, 238, 394
BspPI GGATC 3 cut(s) 149, 231, 244
BsrBI CCGCTC 1 cut(s) 139
BsrFI RCCGGY 2 cut(s) 74, 195
BsrI ACTGG 1 cut(s) 394
BssAI RCCGGY 2 cut(s) 74, 195
BssECI CCNNGG 5 cut(s) 107, 157, 232, 240, 499
BssMI GATC 4 cut(s) 132, 141, 210, 236
BssT1I CCWWGG 2 cut(s) 232, 240
Bst4CI ACNGT 6 cut(s) 46, 67, 228, 307, 533, 544
BstBAI YACGTR 2 cut(s) 124, 181
BstC8I GCNNGC 2 cut(s) 76, 332
BstDSI CCRYGG 2 cut(s) 157, 232
BstFNI CGCG 2 cut(s) 159, 341
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 4 cut(s) 135, 144, 213, 239
BstMAI GTCTC 4 cut(s) 16, 217, 539, 555
BstMBI GATC 4 cut(s) 132, 141, 210, 236
BstMCI CGRYCG 4 cut(s) 98, 200, 213, 220
BstMWI GCNNNNNNNGC 2 cut(s) 103, 112
BstSCI CCNGG 1 cut(s) 106
BstSLI GKGCMC 2 cut(s) 180, 312
BstUI CGCG 2 cut(s) 159, 341
BstX2I RGATCY 1 cut(s) 236
BstXI CCANNNNNNTGG 1 cut(s) 545
BstYI RGATCY 1 cut(s) 236
BstZI CGGCCG 3 cut(s) 95, 197, 217
BsuI GTATCC 1 cut(s) 542
BsuRI GGCC 3 cut(s) 97, 199, 219
BtgI CCRYGG 2 cut(s) 157, 232
BtrI CACGTC 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 42, 473
Cac8I GCNNGC 2 cut(s) 76, 332
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 2 cut(s) 74, 195
Cfr42I CCGCGG 1 cut(s) 160
CseI GACGC 1 cut(s) 347
Csp6I GTAC 3 cut(s) 190, 303, 421
CviAII CATG 2 cut(s) 233, 460
CviQI GTAC 3 cut(s) 190, 303, 421
DpnI GATC 4 cut(s) 134, 143, 212, 238
DpnII GATC 4 cut(s) 132, 141, 210, 236
DraIII CACNNNGTG 1 cut(s) 271
DriI GACNNNNNGTC 1 cut(s) 568
EaeI YGGCCR 3 cut(s) 95, 197, 217
EagI CGGCCG 3 cut(s) 95, 197, 217
Eam1105I GACNNNNNGTC 1 cut(s) 568
EciI GGCGGA 1 cut(s) 164
EclXI CGGCCG 3 cut(s) 95, 197, 217
Eco130I CCWWGG 2 cut(s) 232, 240
Eco52I CGGCCG 3 cut(s) 95, 197, 217
Eco72I CACGTG 1 cut(s) 181
Eco88I CYCGRG 1 cut(s) 498
EcoRI GAATTC 1 cut(s) 521
EcoT14I CCWWGG 2 cut(s) 232, 240
ErhI CCWWGG 2 cut(s) 232, 240
FaeI CATG 2 cut(s) 236, 463
FaiI YATR 6 cut(s) 234, 290, 327, 378, 461, 529
FaqI GGGAC 1 cut(s) 517
FatI CATG 2 cut(s) 232, 459
Fnu4HI GCNGC 3 cut(s) 95, 157, 217
Fsp4HI GCNGC 3 cut(s) 95, 157, 217
GlaI GCGC 1 cut(s) 280
GluI GCNGC 3 cut(s) 95, 157, 217
GsuI CTGGAG 1 cut(s) 521
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 3 cut(s) 97, 199, 219
HapII CCGG 6 cut(s) 75, 107, 196, 200, 220, 575
HgaI GACGC 1 cut(s) 347
HhaI GCGC 1 cut(s) 281
Hin1II CATG 2 cut(s) 236, 463
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HinfI GANTC 2 cut(s) 206, 411
HpaII CCGG 6 cut(s) 75, 107, 196, 200, 220, 575
Hpy166II GTNNAC 4 cut(s) 178, 303, 310, 421
Hpy188I TCNGA 5 cut(s) 21, 71, 90, 103, 259
Hpy188III TCNNGA 3 cut(s) 404, 498, 538
Hpy8I GTNNAC 4 cut(s) 178, 303, 310, 421
Hpy99I CGWCG 5 cut(s) 103, 272, 341, 356, 574
HpyAV CCTTC 2 cut(s) 406, 467
HpyCH4III ACNGT 6 cut(s) 46, 67, 228, 307, 533, 544
HpyCH4IV ACGT 6 cut(s) 123, 168, 180, 270, 314, 569
HpyCH4V TGCA 4 cut(s) 178, 274, 310, 426
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 112
HpySE526I ACGT 6 cut(s) 123, 168, 180, 270, 314, 569
Hsp92II CATG 2 cut(s) 236, 463
HspAI GCGC 1 cut(s) 279
KroI GCCGGC 1 cut(s) 74
KroNI GCCGGC 1 cut(s) 76
KspI CCGCGG 1 cut(s) 160
Kzo9I GATC 4 cut(s) 132, 141, 210, 236
LmnI GCTCC 3 cut(s) 22, 103, 161
LweI GCATC 2 cut(s) 81, 426
MaeII ACGT 6 cut(s) 123, 168, 180, 270, 314, 569
MalI GATC 4 cut(s) 134, 143, 212, 238
MbiI CCGCTC 1 cut(s) 139
MboI GATC 4 cut(s) 132, 141, 210, 236
MboII GAAGA 1 cut(s) 142
MflI RGATCY 1 cut(s) 236
MhlI GDGCHC 2 cut(s) 180, 312
MluCI AATT 2 cut(s) 379, 521
MlyI GAGTC 2 cut(s) 215, 420
MmeI TCCRAC 5 cut(s) 81, 282, 325, 496, 526
MnlI CCTC 8 cut(s) 41, 139, 196, 238, 286, 467, 494, 571
MroNI GCCGGC 1 cut(s) 74
MspA1I CMGCKG 1 cut(s) 159
MspI CCGG 6 cut(s) 75, 107, 196, 200, 220, 575
MspR9I CCNGG 1 cut(s) 108
MvnI CGCG 2 cut(s) 159, 341
MwoI GCNNNNNNNGC 2 cut(s) 103, 112
NaeI GCCGGC 1 cut(s) 76
NciI CCSGG 1 cut(s) 108
NcoI CCATGG 1 cut(s) 232
NdeII GATC 4 cut(s) 132, 141, 210, 236
NgoMIV GCCGGC 1 cut(s) 74
NlaIII CATG 2 cut(s) 236, 463
NlaIV GGNNCC 4 cut(s) 105, 163, 238, 394
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 311
PdiI GCCGGC 1 cut(s) 76
PflFI GACNNNGTC 1 cut(s) 410
PkrI GCNGC 3 cut(s) 96, 158, 218
Ple19I CGATCG 1 cut(s) 213
PleI GAGTC 2 cut(s) 214, 419
PmaCI CACGTG 1 cut(s) 181
PmlI CACGTG 1 cut(s) 181
PpsI GAGTC 2 cut(s) 214, 419
Ppu21I YACGTR 2 cut(s) 124, 181
PspCI CACGTG 1 cut(s) 181
PspN4I GGNNCC 4 cut(s) 105, 163, 238, 394
PsuI RGATCY 1 cut(s) 236
PsyI GACNNNGTC 1 cut(s) 410
PvuI CGATCG 1 cut(s) 213
RsaI GTAC 3 cut(s) 191, 304, 422
RsaNI GTAC 3 cut(s) 190, 303, 421
SacII CCGCGG 1 cut(s) 160
SatI GCNGC 3 cut(s) 95, 157, 217
Sau3AI GATC 4 cut(s) 132, 141, 210, 236
SchI GAGTC 2 cut(s) 215, 420
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 2 cut(s) 180, 312
SfaNI GCATC 2 cut(s) 81, 426
Sfr303I CCGCGG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 160
Sse9I AATT 2 cut(s) 379, 521
SsiI CCGC 8 cut(s) 94, 139, 149, 157, 159, 184, 216, 341
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 2 cut(s) 232, 240
TaaI ACNGT 6 cut(s) 46, 67, 228, 307, 533, 544
TaiI ACGT 6 cut(s) 126, 171, 183, 273, 317, 572
TaqI TCGA 4 cut(s) 135, 144, 209, 336
TaqII GACCGA 1 cut(s) 423
TasI AATT 2 cut(s) 379, 521
TatI WGTACW 1 cut(s) 420
TauI GCSGC 3 cut(s) 97, 159, 219
TscAI CASTG 2 cut(s) 49, 473
TspRI CASTG 2 cut(s) 49, 473
Tth111I GACNNNGTC 1 cut(s) 410
VneI GTGCAC 2 cut(s) 176, 308
XapI RAATTY 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.