Rh6CG253400

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0007718
Physical Location & Seq
Reverse (-)
42358100 .. 42358777
678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG253400.1

Sequence Viewer

Length: 678 bp
ATGGCCTTAAAAATGCGAAGAAAAAGTAGCTTAACCAAGTGGAGGTGGTCTCAACAGCAACCACAATTACCAGAAGACAGGAAGAGCAATGCCTTCTTCTTCATACGGAGCTCTAAATACGGAAGCAATAGTATTAGCACTGATAGTGGAAAGGGATATCTGGAATCTACGGACCAAGAGTTGATGAGCCAGTGCGAAATGGGCACTTTCAAGACGTCAGGGCCGGGGGGTCAGCATCGCAACAAGCGAGAGTCTGCAGTACGCCTTAAGCATCTCCCAACTGGTATTATTGCACAGGCTTCTGAGGATCGATCCCAGCACATGAATCGTGCCTCAGCTTTGTCTCGCCTACGCACTCTTATAGCTCTCAAAGTCAGGAACACTGTGGATCTCGATGGTTATTCCCCTCCTCGAGAGCTTCTTCAAATTCTTCCCAAATCATCTTCCATTAGAACATCAGATATTGGTAACCAAATTGGACCGAACAATCCGAAATTTGTCTACGGAATGCAAGCTCTGTTGGATCTCCTTTATGCAGTTGATGGTTCTGTCTCAGAGGCAGCAAAGTTTCTGGGGTTGAGCACAGGTGCACTGTCTCGATTAATACTCTCTGATGATTCTCTCCGATTGGCAGTCAATGAGCTTAGGGCTTCTAAGGACATGAAGCCTTTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

225

Amino Acids

25.03

Weight (kDa)

10.07

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 63 - 122 2.3e-19 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 218
AccI GTMKAC 1 cut(s) 501
AclWI GGATC 4 cut(s) 306, 315, 396, 531
AcsI RAATTY 2 cut(s) 426, 494
AcyI GRCGYC 1 cut(s) 215
AfaI GTAC 1 cut(s) 261
AfiI CCNNNNNNNGG 1 cut(s) 42
AflII CTTAAG 1 cut(s) 266
AgsI TTSAA 2 cut(s) 211, 425
AluBI AGCT 7 cut(s) 30, 111, 338, 365, 418, 515, 643
AluI AGCT 7 cut(s) 30, 111, 338, 365, 418, 515, 643
Alw21I GWGCWC 3 cut(s) 113, 584, 592
Alw26I GTCTC 4 cut(s) 54, 348, 556, 600
Alw44I GTGCAC 1 cut(s) 588
AlwI GGATC 4 cut(s) 306, 315, 396, 531
Ama87I CYCGRG 1 cut(s) 411
AoxI GGCC 2 cut(s) 3, 221
ApaLI GTGCAC 1 cut(s) 588
ApeKI GCWGC 1 cut(s) 560
ApoI RAATTY 2 cut(s) 426, 494
AseI ATTAAT 1 cut(s) 602
AspS9I GGNCC 3 cut(s) 172, 221, 479
AsuC2I CCSGG 1 cut(s) 225
AvaI CYCGRG 1 cut(s) 411
AvaII GGWCC 2 cut(s) 172, 479
BaeGI GKGCMC 2 cut(s) 206, 592
BanII GRGCYC 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 3 cut(s) 113, 584, 592
BbvCI CCTCAGC 1 cut(s) 334
BbvI GCAGC 1 cut(s) 572
BccI CCATC 2 cut(s) 389, 536
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BcnI CCSGG 1 cut(s) 225
BcoDI GTCTC 4 cut(s) 54, 348, 556, 600
BfmI CTRYAG 1 cut(s) 255
BfrI CTTAAG 1 cut(s) 266
BisI GCNGC 1 cut(s) 561
BlsI GCNGC 1 cut(s) 562
Bme1390I CCNGG 1 cut(s) 225
Bme18I GGWCC 2 cut(s) 172, 479
BmeT110I CYCGRG 1 cut(s) 411
BmgT120I GGNCC 3 cut(s) 172, 221, 479
BmrFI CCNGG 1 cut(s) 225
BmsI GCATC 2 cut(s) 244, 280
BpiI GAAGAC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 34, 66
Bpu10I CCTNAGC 2 cut(s) 334, 644
BpuMI CCSGG 1 cut(s) 225
Bsa29I ATCGAT 1 cut(s) 310
BsaHI GRCGYC 1 cut(s) 215
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 224
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse1I ACTGG 2 cut(s) 190, 286
Bse3DI GCAATG 1 cut(s) 94
BseCI ATCGAT 1 cut(s) 310
BseDI CCNNGG 1 cut(s) 224
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMI GCAATG 1 cut(s) 94
BseMII CTCAG 3 cut(s) 294, 348, 567
BseNI ACTGG 2 cut(s) 190, 286
BseRI GAGGAG 1 cut(s) 399
BseSI GKGCMC 2 cut(s) 206, 592
BseXI GCAGC 1 cut(s) 572
BseYI CCCAGC 1 cut(s) 315
BshFI GGCC 2 cut(s) 5, 223
BshVI ATCGAT 1 cut(s) 310
BsiHKAI GWGCWC 3 cut(s) 113, 584, 592
BsiHKCI CYCGRG 1 cut(s) 411
BsiSI CCGG 1 cut(s) 224
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 4 cut(s) 54, 348, 556, 600
BsmI GAATGC 1 cut(s) 513
BsnI GGCC 2 cut(s) 5, 223
Bso31I GGTCTC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 411
Bsp1286I GDGCHC 4 cut(s) 113, 206, 584, 592
Bsp143I GATC 4 cut(s) 307, 311, 388, 523
BspANI GGCC 2 cut(s) 5, 223
BspCNI CTCAG 3 cut(s) 295, 347, 566
BspDI ATCGAT 1 cut(s) 310
BspMAI CTGCAG 1 cut(s) 259
BspPI GGATC 4 cut(s) 306, 315, 396, 531
BspQI GCTCTTC 1 cut(s) 77
BspTI CTTAAG 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 94
BsrI ACTGG 2 cut(s) 190, 286
BssECI CCNNGG 1 cut(s) 224
BssMI GATC 4 cut(s) 307, 311, 388, 523
BssNI GRCGYC 1 cut(s) 215
Bst4CI ACNGT 2 cut(s) 385, 594
Bst6I CTCTTC 1 cut(s) 77
BstACI GRCGYC 1 cut(s) 215
BstAFI CTTAAG 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 513
BstDEI CTNAG 5 cut(s) 303, 334, 553, 644, 654
BstEII GGTNACC 1 cut(s) 467
BstKTI GATC 4 cut(s) 310, 314, 391, 526
BstMAI GTCTC 4 cut(s) 54, 348, 556, 600
BstMBI GATC 4 cut(s) 307, 311, 388, 523
BstMWI GCNNNNNNNGC 1 cut(s) 201
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 223
BstSFI CTRYAG 1 cut(s) 255
BstSLI GKGCMC 2 cut(s) 206, 592
BstV1I GCAGC 1 cut(s) 572
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 2 cut(s) 388, 523
BstYI RGATCY 2 cut(s) 388, 523
Bsu15I ATCGAT 1 cut(s) 310
BsuRI GGCC 2 cut(s) 5, 223
BsuTUI ATCGAT 1 cut(s) 310
BtgZI GCGATG 1 cut(s) 221
BtsIMutI CAGTG 4 cut(s) 138, 197, 381, 590
Cac8I GCNNGC 1 cut(s) 513
Cfr13I GGNCC 3 cut(s) 172, 221, 479
ClaI ATCGAT 1 cut(s) 310
Csp6I GTAC 1 cut(s) 260
CviAII CATG 2 cut(s) 322, 661
CviQI GTAC 1 cut(s) 260
DdeI CTNAG 5 cut(s) 303, 334, 553, 644, 654
DpnI GATC 4 cut(s) 309, 313, 390, 525
DpnII GATC 4 cut(s) 307, 311, 388, 523
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Ecl136II GAGCTC 1 cut(s) 111
Eco24I GRGCYC 1 cut(s) 113
Eco31I GGTCTC 1 cut(s) 54
Eco32I GATATC 1 cut(s) 158
Eco47I GGWCC 2 cut(s) 172, 479
Eco53kI GAGCTC 1 cut(s) 111
Eco88I CYCGRG 1 cut(s) 411
Eco91I GGTNACC 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 111
EcoO65I GGTNACC 1 cut(s) 467
EcoRV GATATC 1 cut(s) 158
EcoT38I GRGCYC 1 cut(s) 113
FaeI CATG 2 cut(s) 325, 664
FaiI YATR 5 cut(s) 104, 323, 362, 534, 662
FatI CATG 2 cut(s) 321, 660
FblI GTMKAC 1 cut(s) 501
Fnu4HI GCNGC 1 cut(s) 561
FriOI GRGCYC 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 561
GluI GCNGC 1 cut(s) 561
GsaI CCCAGC 1 cut(s) 319
HaeIII GGCC 2 cut(s) 5, 223
HapII CCGG 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 215
Hin1II CATG 2 cut(s) 325, 664
HinfI GANTC 4 cut(s) 164, 251, 325, 617
HpaII CCGG 1 cut(s) 224
Hpy166II GTNNAC 2 cut(s) 502, 590
Hpy188I TCNGA 6 cut(s) 304, 460, 492, 556, 613, 626
Hpy188III TCNNGA 6 cut(s) 161, 211, 376, 392, 413, 597
Hpy8I GTNNAC 2 cut(s) 502, 590
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 2 cut(s) 385, 594
HpyCH4IV ACGT 1 cut(s) 215
HpyCH4V TGCA 5 cut(s) 257, 293, 511, 536, 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 201
HpyF3I CTNAG 5 cut(s) 303, 334, 553, 644, 654
HpySE526I ACGT 1 cut(s) 215
Hsp92I GRCGYC 1 cut(s) 215
Hsp92II CATG 2 cut(s) 325, 664
Kzo9I GATC 4 cut(s) 307, 311, 388, 523
LguI GCTCTTC 1 cut(s) 77
LmnI GCTCC 1 cut(s) 108
Lsp1109I GCAGC 1 cut(s) 572
LweI GCATC 2 cut(s) 244, 280
MaeII ACGT 1 cut(s) 215
MaeIII GTNAC 1 cut(s) 467
MalI GATC 4 cut(s) 309, 313, 390, 525
MboI GATC 4 cut(s) 307, 311, 388, 523
MboII GAAGA 8 cut(s) 30, 86, 88, 91, 94, 413, 422, 435
MflI RGATCY 2 cut(s) 388, 523
MhlI GDGCHC 4 cut(s) 113, 206, 584, 592
MluCI AATT 4 cut(s) 65, 426, 474, 494
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 7 cut(s) 36, 298, 343, 417, 420, 550, 666
MseI TTAA 4 cut(s) 8, 32, 267, 602
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 1 cut(s) 224
MspR9I CCNGG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 513
MwoI GCNNNNNNNGC 1 cut(s) 201
NciI CCSGG 1 cut(s) 225
NdeII GATC 4 cut(s) 307, 311, 388, 523
NlaIII CATG 2 cut(s) 325, 664
PaeR7I CTCGAG 1 cut(s) 411
PciSI GCTCTTC 1 cut(s) 77
PcsI WCGNNNNNNNCGW 1 cut(s) 244
PctI GAATGC 1 cut(s) 513
PfeI GAWTC 3 cut(s) 164, 325, 617
PkrI GCNGC 1 cut(s) 562
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PshBI ATTAAT 1 cut(s) 602
Psp124BI GAGCTC 1 cut(s) 113
PspEI GGTNACC 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 315
PspPI GGNCC 3 cut(s) 172, 221, 479
PstI CTGCAG 1 cut(s) 259
PsuI RGATCY 2 cut(s) 388, 523
RsaI GTAC 1 cut(s) 261
RsaNI GTAC 1 cut(s) 260
SacI GAGCTC 1 cut(s) 113
SapI GCTCTTC 1 cut(s) 77
SaqAI TTAA 4 cut(s) 8, 32, 267, 602
SatI GCNGC 1 cut(s) 561
Sau3AI GATC 4 cut(s) 307, 311, 388, 523
Sau96I GGNCC 3 cut(s) 172, 221, 479
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 225
SduI GDGCHC 4 cut(s) 113, 206, 584, 592
SfaNI GCATC 2 cut(s) 244, 280
SfcI CTRYAG 1 cut(s) 255
Sfr274I CTCGAG 1 cut(s) 411
SinI GGWCC 2 cut(s) 172, 479
SlaI CTCGAG 1 cut(s) 411
SmlI CTYRAG 2 cut(s) 266, 411
SmoI CTYRAG 2 cut(s) 266, 411
Sse9I AATT 4 cut(s) 65, 426, 474, 494
SstI GAGCTC 1 cut(s) 113
StyD4I CCNGG 1 cut(s) 223
TaaI ACNGT 2 cut(s) 385, 594
TaiI ACGT 1 cut(s) 218
TaqI TCGA 4 cut(s) 310, 393, 412, 598
TaqII GACCGA 1 cut(s) 496
TasI AATT 4 cut(s) 65, 426, 474, 494
TfiI GAWTC 3 cut(s) 164, 325, 617
Tru1I TTAA 4 cut(s) 8, 32, 267, 602
Tru9I TTAA 4 cut(s) 8, 32, 267, 602
TscAI CASTG 4 cut(s) 145, 197, 388, 597
TseI GCWGC 1 cut(s) 560
TspDTI ATGAA 3 cut(s) 91, 338, 677
TspGWI ACGGA 4 cut(s) 121, 135, 185, 519
TspRI CASTG 4 cut(s) 145, 197, 388, 597
Vha464I CTTAAG 1 cut(s) 266
VneI GTGCAC 1 cut(s) 588
VpaK11BI GGWCC 2 cut(s) 172, 479
VspI ATTAAT 1 cut(s) 602
XapI RAATTY 2 cut(s) 426, 494
XhoI CTCGAG 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 501
ZraI GACGTC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.