Rh6DG245000

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0007718
Physical Location & Seq
Reverse (-)
43186528 .. 43187205
678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG245000.1

Sequence Viewer

Length: 678 bp
ATGGCCTTAAAAATACAAAGAAAAAGTAGCTTAACCAAGTGGAGGTGGTCTCAACAGCAACCACAATTACCAGAAGACAGGAACAGCAATGCCTTCTTCTTCATACGGAGCTCTAAATACGGAACCAATAGTATTAGCACTGATGGCGGAAAGGGATATTTGGAATCTACAGACCAAGAGTTGATGAGCCAGTGCGAAATGGGCACTTTCAAGACGTCAGGGCCAGGGGGTCAGCACCGCAACAAGCGAGAGTCTGCAGTACGCCTTAAGCATCTCCCAACTGGTATTATTGCACAGGCTTCTGAGGATCGATCCCAGCACATGAATCGTGCCTCAGCTTTATCTCGCCTGCGCACTCTTATAGCTCTCAAAGTCAGGAACGCTGTGGATCTCGATGGTTATTCCCCTCCTCGAGAGCTTCTTCAAATTCTTCCCAAATCATCTTCCATTAGAACATCAGATATTGGTAACCAAATTGGACCGAACAATCCGAAATTTGTTTACGGAATGCAAGCTCTGTTGGATCTCCTTTATGCAGTTGATGGTTCTGTCTCAGAGGCAGCAAAGTTTCTGGGGTTGAGCACAGGTGCACTGTCTCGATTAATACTCTCTGATGATTCTCTCCGATTGGCAGTCAATGAGCTTAGGGCTTCTGAGGACATGAAGCCTTTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

225

Amino Acids

24.93

Weight (kDa)

9.81

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 63 - 122 2.2e-19 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 218
Acc16I TGCGCA 1 cut(s) 353
AciI CCGC 2 cut(s) 147, 238
AclWI GGATC 4 cut(s) 306, 315, 396, 531
AcsI RAATTY 2 cut(s) 426, 494
AcyI GRCGYC 1 cut(s) 215
AfaI GTAC 1 cut(s) 261
AfiI CCNNNNNNNGG 1 cut(s) 42
AflII CTTAAG 1 cut(s) 266
AgsI TTSAA 2 cut(s) 211, 425
AjnI CCWGG 1 cut(s) 223
AluBI AGCT 7 cut(s) 30, 111, 338, 365, 418, 515, 643
AluI AGCT 7 cut(s) 30, 111, 338, 365, 418, 515, 643
Alw21I GWGCWC 3 cut(s) 113, 584, 592
Alw26I GTCTC 3 cut(s) 54, 556, 600
Alw44I GTGCAC 1 cut(s) 588
AlwI GGATC 4 cut(s) 306, 315, 396, 531
Ama87I CYCGRG 1 cut(s) 411
AoxI GGCC 2 cut(s) 3, 221
ApaLI GTGCAC 1 cut(s) 588
ApeKI GCWGC 1 cut(s) 560
ApoI RAATTY 2 cut(s) 426, 494
AseI ATTAAT 1 cut(s) 602
AspLEI GCGC 1 cut(s) 354
AspS9I GGNCC 2 cut(s) 221, 479
AvaI CYCGRG 1 cut(s) 411
AvaII GGWCC 1 cut(s) 479
BaeGI GKGCMC 2 cut(s) 206, 592
BanII GRGCYC 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 3 cut(s) 113, 584, 592
BbvCI CCTCAGC 1 cut(s) 334
BbvI GCAGC 1 cut(s) 572
BccI CCATC 3 cut(s) 137, 389, 536
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 1 cut(s) 225
BcoDI GTCTC 3 cut(s) 54, 556, 600
BfmI CTRYAG 2 cut(s) 168, 255
BfrI CTTAAG 1 cut(s) 266
BisI GCNGC 1 cut(s) 561
BlsI GCNGC 1 cut(s) 562
Bme1390I CCNGG 1 cut(s) 225
Bme18I GGWCC 1 cut(s) 479
BmeT110I CYCGRG 1 cut(s) 411
BmgT120I GGNCC 2 cut(s) 221, 479
BmiI GGNNCC 1 cut(s) 124
BmrFI CCNGG 1 cut(s) 225
BmsI GCATC 1 cut(s) 280
BpiI GAAGAC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 34, 66
Bpu10I CCTNAGC 2 cut(s) 334, 644
Bsa29I ATCGAT 1 cut(s) 310
BsaHI GRCGYC 1 cut(s) 215
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 224
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse1I ACTGG 2 cut(s) 190, 286
Bse3DI GCAATG 1 cut(s) 94
BseBI CCWGG 1 cut(s) 225
BseCI ATCGAT 1 cut(s) 310
BseDI CCNNGG 1 cut(s) 224
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMI GCAATG 1 cut(s) 94
BseMII CTCAG 4 cut(s) 294, 348, 567, 645
BseNI ACTGG 2 cut(s) 190, 286
BseRI GAGGAG 1 cut(s) 399
BseSI GKGCMC 2 cut(s) 206, 592
BseXI GCAGC 1 cut(s) 572
BseYI CCCAGC 1 cut(s) 315
BshFI GGCC 2 cut(s) 5, 223
BshVI ATCGAT 1 cut(s) 310
BsiHKAI GWGCWC 3 cut(s) 113, 584, 592
BsiHKCI CYCGRG 1 cut(s) 411
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 3 cut(s) 54, 556, 600
BsmI GAATGC 1 cut(s) 513
BsnI GGCC 2 cut(s) 5, 223
Bso31I GGTCTC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 411
Bsp1286I GDGCHC 4 cut(s) 113, 206, 584, 592
Bsp143I GATC 4 cut(s) 307, 311, 388, 523
BspACI CCGC 2 cut(s) 147, 238
BspANI GGCC 2 cut(s) 5, 223
BspCNI CTCAG 4 cut(s) 295, 347, 566, 646
BspDI ATCGAT 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 124
BspMAI CTGCAG 1 cut(s) 259
BspPI GGATC 4 cut(s) 306, 315, 396, 531
BspTI CTTAAG 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 94
BsrI ACTGG 2 cut(s) 190, 286
BssECI CCNNGG 1 cut(s) 224
BssMI GATC 4 cut(s) 307, 311, 388, 523
BssNI GRCGYC 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 594
BstACI GRCGYC 1 cut(s) 215
BstAFI CTTAAG 1 cut(s) 266
BstC8I GCNNGC 2 cut(s) 350, 513
BstDEI CTNAG 5 cut(s) 303, 334, 553, 644, 654
BstEII GGTNACC 1 cut(s) 467
BstHHI GCGC 1 cut(s) 354
BstKTI GATC 4 cut(s) 310, 314, 391, 526
BstMAI GTCTC 3 cut(s) 54, 556, 600
BstMBI GATC 4 cut(s) 307, 311, 388, 523
BstMWI GCNNNNNNNGC 2 cut(s) 144, 201
BstNI CCWGG 1 cut(s) 225
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 223
BstSFI CTRYAG 2 cut(s) 168, 255
BstSLI GKGCMC 2 cut(s) 206, 592
BstV1I GCAGC 1 cut(s) 572
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 2 cut(s) 388, 523
BstYI RGATCY 2 cut(s) 388, 523
Bsu15I ATCGAT 1 cut(s) 310
BsuRI GGCC 2 cut(s) 5, 223
BsuTUI ATCGAT 1 cut(s) 310
BtsIMutI CAGTG 3 cut(s) 138, 197, 590
Cac8I GCNNGC 2 cut(s) 350, 513
CfoI GCGC 1 cut(s) 354
Cfr13I GGNCC 2 cut(s) 221, 479
ClaI ATCGAT 1 cut(s) 310
Csp6I GTAC 1 cut(s) 260
CviAII CATG 2 cut(s) 322, 661
CviQI GTAC 1 cut(s) 260
DdeI CTNAG 5 cut(s) 303, 334, 553, 644, 654
DpnI GATC 4 cut(s) 309, 313, 390, 525
DpnII GATC 4 cut(s) 307, 311, 388, 523
EciI GGCGGA 1 cut(s) 162
Ecl136II GAGCTC 1 cut(s) 111
Eco24I GRGCYC 1 cut(s) 113
Eco31I GGTCTC 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 479
Eco53kI GAGCTC 1 cut(s) 111
Eco88I CYCGRG 1 cut(s) 411
Eco91I GGTNACC 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 111
EcoO65I GGTNACC 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 223
EcoT38I GRGCYC 1 cut(s) 113
FaeI CATG 2 cut(s) 325, 664
FaiI YATR 5 cut(s) 104, 323, 362, 534, 662
FatI CATG 2 cut(s) 321, 660
Fnu4HI GCNGC 1 cut(s) 561
FriOI GRGCYC 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 561
FspI TGCGCA 1 cut(s) 353
GlaI GCGC 1 cut(s) 353
GluI GCNGC 1 cut(s) 561
GsaI CCCAGC 1 cut(s) 319
HaeIII GGCC 2 cut(s) 5, 223
HhaI GCGC 1 cut(s) 354
Hin1I GRCGYC 1 cut(s) 215
Hin1II CATG 2 cut(s) 325, 664
Hin6I GCGC 1 cut(s) 352
HinP1I GCGC 1 cut(s) 352
HinfI GANTC 4 cut(s) 164, 251, 325, 617
Hpy166II GTNNAC 2 cut(s) 502, 590
Hpy188I TCNGA 7 cut(s) 304, 460, 492, 556, 613, 626, 655
Hpy188III TCNNGA 5 cut(s) 211, 376, 392, 413, 597
Hpy8I GTNNAC 2 cut(s) 502, 590
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 1 cut(s) 594
HpyCH4IV ACGT 1 cut(s) 215
HpyCH4V TGCA 5 cut(s) 257, 293, 511, 536, 590
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 201
HpyF3I CTNAG 5 cut(s) 303, 334, 553, 644, 654
HpySE526I ACGT 1 cut(s) 215
Hsp92I GRCGYC 1 cut(s) 215
Hsp92II CATG 2 cut(s) 325, 664
HspAI GCGC 1 cut(s) 352
Kzo9I GATC 4 cut(s) 307, 311, 388, 523
LmnI GCTCC 1 cut(s) 108
Lsp1109I GCAGC 1 cut(s) 572
LweI GCATC 1 cut(s) 280
MaeII ACGT 1 cut(s) 215
MaeIII GTNAC 1 cut(s) 467
MalI GATC 4 cut(s) 309, 313, 390, 525
MboI GATC 4 cut(s) 307, 311, 388, 523
MboII GAAGA 6 cut(s) 86, 88, 91, 413, 422, 435
MflI RGATCY 2 cut(s) 388, 523
MhlI GDGCHC 4 cut(s) 113, 206, 584, 592
MluCI AATT 4 cut(s) 65, 426, 474, 494
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 8 cut(s) 36, 298, 343, 417, 420, 550, 649, 666
MseI TTAA 4 cut(s) 8, 32, 267, 602
MspCI CTTAAG 1 cut(s) 266
MspR9I CCNGG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 513
MvaI CCWGG 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 144, 201
NdeII GATC 4 cut(s) 307, 311, 388, 523
NlaIII CATG 2 cut(s) 325, 664
NlaIV GGNNCC 1 cut(s) 124
NsbI TGCGCA 1 cut(s) 353
PaeR7I CTCGAG 1 cut(s) 411
PctI GAATGC 1 cut(s) 513
PfeI GAWTC 3 cut(s) 164, 325, 617
PkrI GCNGC 1 cut(s) 562
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PshBI ATTAAT 1 cut(s) 602
Psp124BI GAGCTC 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 223
PspEI GGTNACC 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 315
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 124
PspPI GGNCC 2 cut(s) 221, 479
PstI CTGCAG 1 cut(s) 259
PsuI RGATCY 2 cut(s) 388, 523
RsaI GTAC 1 cut(s) 261
RsaNI GTAC 1 cut(s) 260
SacI GAGCTC 1 cut(s) 113
SaqAI TTAA 4 cut(s) 8, 32, 267, 602
SatI GCNGC 1 cut(s) 561
Sau3AI GATC 4 cut(s) 307, 311, 388, 523
Sau96I GGNCC 2 cut(s) 221, 479
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 225
SduI GDGCHC 4 cut(s) 113, 206, 584, 592
SfaNI GCATC 1 cut(s) 280
SfcI CTRYAG 2 cut(s) 168, 255
Sfr274I CTCGAG 1 cut(s) 411
SinI GGWCC 1 cut(s) 479
SlaI CTCGAG 1 cut(s) 411
SmlI CTYRAG 2 cut(s) 266, 411
SmoI CTYRAG 2 cut(s) 266, 411
Sse9I AATT 4 cut(s) 65, 426, 474, 494
SsiI CCGC 2 cut(s) 147, 238
SstI GAGCTC 1 cut(s) 113
StyD4I CCNGG 1 cut(s) 223
TaaI ACNGT 1 cut(s) 594
TaiI ACGT 1 cut(s) 218
TaqI TCGA 4 cut(s) 310, 393, 412, 598
TaqII GACCGA 1 cut(s) 496
TasI AATT 4 cut(s) 65, 426, 474, 494
TfiI GAWTC 3 cut(s) 164, 325, 617
Tru1I TTAA 4 cut(s) 8, 32, 267, 602
Tru9I TTAA 4 cut(s) 8, 32, 267, 602
TscAI CASTG 3 cut(s) 145, 197, 597
TseI GCWGC 1 cut(s) 560
TspDTI ATGAA 3 cut(s) 91, 338, 677
TspGWI ACGGA 3 cut(s) 121, 135, 519
TspRI CASTG 3 cut(s) 145, 197, 597
Vha464I CTTAAG 1 cut(s) 266
VneI GTGCAC 1 cut(s) 588
VpaK11BI GGWCC 1 cut(s) 479
VspI ATTAAT 1 cut(s) 602
XapI RAATTY 2 cut(s) 426, 494
XhoI CTCGAG 1 cut(s) 411
ZraI GACGTC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.