Prupe.3G000100_v2.0.a1

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0007718
Physical Location & Seq
Forward (+)
13662 .. 18509
4848 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G000100.3

Sequence Viewer

Length: 822 bp
ATGGCCCTGGCACAGTCAGGGTGGAAATTATCATATTCAAATTCATGGGTTAGAGTAGTTGGAGTTTCAAGAAGAAGCAGTGGCGCAGCTGCTAAGCCTGCTATATTTCAAGCATCATGTCCACCTCCGTCTCCAGCCATAGCTGTGAAAATAAGAAGAAAATGCTTAAGCAACTGGAGGTGGACGCAACAGCCACCACAATTACTGGAAGACAAGAAAAGCGACGCCTCCTTCTTCATACGGTGCTCTAAGTACGGAAGCAGCATTGGTGCCGCAAGTAATACGAATAGCGGAAAGGGATATCTGGCATCTACGGACCAAGAGCTGATGAGTCAGTGCCAAATGGACACTTTCAAGGCGTCGGGCCCAGGGGGTCAGCACCGTAACAAGCGAGAGTCTGCCGTACGCCTCAAGCACCTCCCCACCGGTCTTGCTGCGCAGGCTGTCGAGGACCGATCCCAACACATGAATCGTGCCTCAGCCTTGGCTCGTCTTCGCGCTCTTATAGCTCTAAAAGTCAGGAACACTGTGGATCTTGATGCTTATTCCCCTCCTCAAGAGCTTCTTCAAATTCTTCCCCAAAAGTCTACCATTAGAGGATCAGATTGTGGTCCCCAAATTGGACCCAATAATCCTAAGTTCATTTTGGGAATGCAAGCTCTGTTGGATCTCATTTTTGCAGTTGAGGGTTCTGTCTCAGAGGCAGCCAAGTTTCTTGGGTTAAGCACAGGTGCCCTGTCACGGTTAATACTCTCAAATGATTCTCTCCGACTGGCAGTCAATGAACTAAGGTTTTCTAAGGGTATGAAGCCTCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

274

Amino Acids

29.58

Weight (kDa)

10.16

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 438
AccB1I GGYRCC 2 cut(s) 269, 731
AccI GTMKAC 1 cut(s) 587
AccII CGCG 1 cut(s) 498
AciI CCGC 2 cut(s) 273, 291
AclWI GGATC 4 cut(s) 450, 540, 607, 675
AcsI RAATTY 2 cut(s) 40, 570
AcyI GRCGYC 2 cut(s) 225, 359
AfaI GTAC 2 cut(s) 254, 405
AfiI CCNNNNNNNGG 2 cut(s) 620, 741
AflII CTTAAG 1 cut(s) 166
AgeI ACCGGT 1 cut(s) 425
AgsI TTSAA 5 cut(s) 39, 69, 110, 355, 569
AhdI GACNNNNNGTC 1 cut(s) 776
AjnI CCWGG 2 cut(s) 6, 367
AluBI AGCT 6 cut(s) 89, 143, 325, 509, 562, 659
AluI AGCT 6 cut(s) 89, 143, 325, 509, 562, 659
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 2 cut(s) 135, 700
AlwI GGATC 4 cut(s) 450, 540, 607, 675
AoxI GGCC 2 cut(s) 3, 364
ApaI GGGCCC 1 cut(s) 368
ApeKI GCWGC 5 cut(s) 86, 89, 261, 434, 704
ApoI RAATTY 2 cut(s) 40, 570
AsiGI ACCGGT 1 cut(s) 425
AspLEI GCGC 3 cut(s) 86, 439, 500
AspS9I GGNCC 7 cut(s) 4, 316, 364, 365, 451, 611, 623
AvaII GGWCC 4 cut(s) 316, 451, 611, 623
BaeGI GKGCMC 2 cut(s) 368, 736
BanI GGYRCC 2 cut(s) 269, 731
BanII GRGCYC 1 cut(s) 368
BbsI GAAGAC 2 cut(s) 216, 485
Bbv12I GWGCWC 1 cut(s) 248
BbvCI CCTCAGC 1 cut(s) 478
BbvI GCAGC 5 cut(s) 76, 98, 273, 421, 716
BceAI ACGGC 1 cut(s) 386
BciT130I CCWGG 2 cut(s) 8, 369
BcoDI GTCTC 2 cut(s) 135, 700
BfrI CTTAAG 1 cut(s) 166
BisI GCNGC 6 cut(s) 87, 90, 262, 273, 435, 705
BlpI GCTNAGC 1 cut(s) 93
BlsI GCNGC 6 cut(s) 88, 91, 263, 274, 436, 706
Bme1390I CCNGG 2 cut(s) 8, 369
Bme18I GGWCC 4 cut(s) 316, 451, 611, 623
BmeRI GACNNNNNGTC 1 cut(s) 776
BmgT120I GGNCC 7 cut(s) 4, 316, 364, 365, 451, 611, 623
BmiI GGNNCC 5 cut(s) 271, 366, 613, 625, 733
BmrFI CCNGG 2 cut(s) 8, 369
BmsI GCATC 3 cut(s) 122, 317, 529
BpiI GAAGAC 2 cut(s) 216, 485
BpmI CTGGAG 2 cut(s) 117, 196
Bpu10I CCTNAGC 1 cut(s) 478
Bpu1102I GCTNAGC 1 cut(s) 93
BpuEI CTTGAG 3 cut(s) 395, 540, 800
BsaHI GRCGYC 2 cut(s) 225, 359
BsaJI CCNNGG 4 cut(s) 6, 367, 368, 483
BsaWI WCCGGW 1 cut(s) 425
Bsc4I CCNNNNNNNGG 2 cut(s) 620, 741
Bse118I RCCGGY 1 cut(s) 425
Bse1I ACTGG 3 cut(s) 179, 210, 777
BseBI CCWGG 2 cut(s) 8, 369
BseDI CCNNGG 4 cut(s) 6, 367, 368, 483
BseLI CCNNNNNNNGG 2 cut(s) 620, 741
BseMII CTCAG 2 cut(s) 492, 711
BseNI ACTGG 3 cut(s) 179, 210, 777
BseRI GAGGAG 1 cut(s) 543
BseSI GKGCMC 2 cut(s) 368, 736
BseXI GCAGC 5 cut(s) 76, 98, 273, 421, 716
Bsh1236I CGCG 1 cut(s) 498
BshFI GGCC 2 cut(s) 5, 366
BshNI GGYRCC 2 cut(s) 269, 731
BshTI ACCGGT 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 248
BsiSI CCGG 1 cut(s) 426
BsiWI CGTACG 1 cut(s) 403
BslFI GGGAC 1 cut(s) 597
BslI CCNNNNNNNGG 2 cut(s) 620, 741
BsmAI GTCTC 2 cut(s) 135, 700
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 597
BsmI GAATGC 1 cut(s) 657
BsnI GGCC 2 cut(s) 5, 366
Bsp120I GGGCCC 1 cut(s) 364
Bsp1286I GDGCHC 3 cut(s) 248, 368, 736
Bsp143I GATC 4 cut(s) 455, 532, 599, 667
Bsp1720I GCTNAGC 1 cut(s) 93
BspACI CCGC 2 cut(s) 273, 291
BspANI GGCC 2 cut(s) 5, 366
BspCNI CTCAG 2 cut(s) 491, 710
BspFNI CGCG 1 cut(s) 498
BspLI GGNNCC 5 cut(s) 271, 366, 613, 625, 733
BspPI GGATC 4 cut(s) 450, 540, 607, 675
BspT107I GGYRCC 2 cut(s) 269, 731
BspTI CTTAAG 1 cut(s) 166
BsrFI RCCGGY 1 cut(s) 425
BsrI ACTGG 3 cut(s) 179, 210, 777
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 4 cut(s) 6, 367, 368, 483
BssMI GATC 4 cut(s) 455, 532, 599, 667
BssNI GRCGYC 2 cut(s) 225, 359
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 2 cut(s) 8, 369
Bst4CI ACNGT 5 cut(s) 15, 243, 383, 529, 744
BstACI GRCGYC 2 cut(s) 225, 359
BstAFI CTTAAG 1 cut(s) 166
BstC8I GCNNGC 3 cut(s) 99, 441, 657
BstDEI CTNAG 7 cut(s) 93, 249, 478, 636, 697, 788, 798
BstFNI CGCG 1 cut(s) 498
BstHHI GCGC 3 cut(s) 86, 439, 500
BstKTI GATC 4 cut(s) 458, 535, 602, 670
BstMAI GTCTC 2 cut(s) 135, 700
BstMBI GATC 4 cut(s) 455, 532, 599, 667
BstMWI GCNNNNNNNGC 3 cut(s) 98, 440, 506
BstNI CCWGG 2 cut(s) 8, 369
BstSCI CCNGG 2 cut(s) 6, 367
BstSLI GKGCMC 2 cut(s) 368, 736
BstUI CGCG 1 cut(s) 498
BstV1I GCAGC 5 cut(s) 76, 98, 273, 421, 716
BstV2I GAAGAC 2 cut(s) 216, 485
BstX2I RGATCY 2 cut(s) 532, 667
BstYI RGATCY 2 cut(s) 532, 667
BsuRI GGCC 2 cut(s) 5, 366
BtsI GCAGTG 1 cut(s) 85
BtsIMutI CAGTG 3 cut(s) 85, 341, 525
Cac8I GCNNGC 3 cut(s) 99, 441, 657
CfoI GCGC 3 cut(s) 86, 439, 500
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 7 cut(s) 4, 316, 364, 365, 451, 611, 623
CseI GACGC 3 cut(s) 193, 233, 348
Csp6I GTAC 2 cut(s) 253, 404
CspAI ACCGGT 1 cut(s) 425
CspCI CAANNNNNGTGG 2 cut(s) 412, 447
CviAII CATG 3 cut(s) 45, 117, 466
CviQI GTAC 2 cut(s) 253, 404
DdeI CTNAG 7 cut(s) 93, 249, 478, 636, 697, 788, 798
DpnI GATC 4 cut(s) 457, 534, 601, 669
DpnII GATC 4 cut(s) 455, 532, 599, 667
DriI GACNNNNNGTC 1 cut(s) 776
Eam1105I GACNNNNNGTC 1 cut(s) 776
Eco130I CCWWGG 1 cut(s) 483
Eco24I GRGCYC 1 cut(s) 368
Eco32I GATATC 1 cut(s) 302
Eco47I GGWCC 4 cut(s) 316, 451, 611, 623
EcoRII CCWGG 2 cut(s) 6, 367
EcoRV GATATC 1 cut(s) 302
EcoT14I CCWWGG 1 cut(s) 483
EcoT38I GRGCYC 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 483
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 3 cut(s) 48, 120, 469
FaiI YATR 9 cut(s) 34, 46, 104, 118, 140, 239, 467, 506, 806
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 1 cut(s) 597
FatI CATG 3 cut(s) 44, 116, 465
FblI GTMKAC 1 cut(s) 587
Fnu4HI GCNGC 6 cut(s) 87, 90, 262, 273, 435, 705
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 6 cut(s) 87, 90, 262, 273, 435, 705
FspI TGCGCA 1 cut(s) 438
GlaI GCGC 3 cut(s) 85, 438, 499
GluI GCNGC 6 cut(s) 87, 90, 262, 273, 435, 705
GsuI CTGGAG 2 cut(s) 117, 196
HaeIII GGCC 2 cut(s) 5, 366
HapII CCGG 1 cut(s) 426
HgaI GACGC 3 cut(s) 193, 233, 348
HhaI GCGC 3 cut(s) 86, 439, 500
Hin1I GRCGYC 2 cut(s) 225, 359
Hin1II CATG 3 cut(s) 48, 120, 469
Hin6I GCGC 3 cut(s) 84, 437, 498
HinP1I GCGC 3 cut(s) 84, 437, 498
HinfI GANTC 4 cut(s) 331, 395, 469, 761
HpaII CCGG 1 cut(s) 426
Hpy166II GTNNAC 3 cut(s) 122, 183, 588
Hpy188I TCNGA 3 cut(s) 604, 700, 770
Hpy188III TCNNGA 4 cut(s) 69, 520, 536, 557
Hpy8I GTNNAC 3 cut(s) 122, 183, 588
Hpy99I CGWCG 2 cut(s) 227, 364
HpyAV CCTTC 1 cut(s) 241
HpyCH4III ACNGT 5 cut(s) 15, 243, 383, 529, 744
HpyCH4V TGCA 2 cut(s) 655, 680
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 440, 506
HpyF3I CTNAG 7 cut(s) 93, 249, 478, 636, 697, 788, 798
Hsp92I GRCGYC 2 cut(s) 225, 359
Hsp92II CATG 3 cut(s) 48, 120, 469
HspAI GCGC 3 cut(s) 84, 437, 498
Kzo9I GATC 4 cut(s) 455, 532, 599, 667
Lsp1109I GCAGC 5 cut(s) 76, 98, 273, 421, 716
LweI GCATC 3 cut(s) 122, 317, 529
MaeIII GTNAC 2 cut(s) 383, 738
MalI GATC 4 cut(s) 457, 534, 601, 669
MboI GATC 4 cut(s) 455, 532, 599, 667
MboII GAAGA 7 cut(s) 84, 168, 221, 226, 485, 557, 566
MflI RGATCY 2 cut(s) 532, 667
MhlI GDGCHC 3 cut(s) 248, 368, 736
MluCI AATT 5 cut(s) 26, 40, 200, 570, 618
MlyI GAGTC 2 cut(s) 340, 404
MmeI TCCRAC 3 cut(s) 40, 645, 793
MseI TTAA 3 cut(s) 167, 722, 746
MslI CAYNNNNRTG 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 89
MspCI CTTAAG 1 cut(s) 166
MspI CCGG 1 cut(s) 426
MspR9I CCNGG 2 cut(s) 8, 369
Mva1269I GAATGC 1 cut(s) 657
MvaI CCWGG 2 cut(s) 8, 369
MvnI CGCG 1 cut(s) 498
MwoI GCNNNNNNNGC 3 cut(s) 98, 440, 506
NdeII GATC 4 cut(s) 455, 532, 599, 667
NlaIII CATG 3 cut(s) 48, 120, 469
NlaIV GGNNCC 5 cut(s) 271, 366, 613, 625, 733
NmuCI GTSAC 1 cut(s) 738
NsbI TGCGCA 1 cut(s) 438
PasI CCCWGGG 1 cut(s) 368
PctI GAATGC 1 cut(s) 657
PfeI GAWTC 2 cut(s) 469, 761
Pfl23II CGTACG 1 cut(s) 403
PinAI ACCGGT 1 cut(s) 425
PkrI GCNGC 6 cut(s) 88, 91, 263, 274, 436, 706
PleI GAGTC 2 cut(s) 339, 403
PpsI GAGTC 2 cut(s) 339, 403
Psp6I CCWGG 2 cut(s) 6, 367
PspGI CCWGG 2 cut(s) 6, 367
PspLI CGTACG 1 cut(s) 403
PspN4I GGNNCC 5 cut(s) 271, 366, 613, 625, 733
PspOMI GGGCCC 1 cut(s) 364
PspPI GGNCC 7 cut(s) 4, 316, 364, 365, 451, 611, 623
PsuI RGATCY 2 cut(s) 532, 667
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 2 cut(s) 254, 405
RsaNI GTAC 2 cut(s) 253, 404
RseI CAYNNNNRTG 1 cut(s) 143
SaqAI TTAA 3 cut(s) 167, 722, 746
SatI GCNGC 6 cut(s) 87, 90, 262, 273, 435, 705
Sau3AI GATC 4 cut(s) 455, 532, 599, 667
Sau96I GGNCC 7 cut(s) 4, 316, 364, 365, 451, 611, 623
SchI GAGTC 2 cut(s) 340, 404
ScrFI CCNGG 2 cut(s) 8, 369
SduI GDGCHC 3 cut(s) 248, 368, 736
SfaNI GCATC 3 cut(s) 122, 317, 529
SinI GGWCC 4 cut(s) 316, 451, 611, 623
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 4 cut(s) 166, 410, 555, 815
SmoI CTYRAG 4 cut(s) 166, 410, 555, 815
Sse9I AATT 5 cut(s) 26, 40, 200, 570, 618
SsiI CCGC 2 cut(s) 273, 291
StyD4I CCNGG 2 cut(s) 6, 367
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 5 cut(s) 15, 243, 383, 529, 744
TaqI TCGA 1 cut(s) 447
TaqII GACCGA 1 cut(s) 468
TasI AATT 5 cut(s) 26, 40, 200, 570, 618
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 2 cut(s) 469, 761
Tru1I TTAA 3 cut(s) 167, 722, 746
Tru9I TTAA 3 cut(s) 167, 722, 746
TscAI CASTG 3 cut(s) 85, 341, 532
TseFI GTSAC 1 cut(s) 738
TseI GCWGC 5 cut(s) 86, 89, 261, 434, 704
Tsp45I GTSAC 1 cut(s) 738
TspDTI ATGAA 6 cut(s) 33, 226, 482, 631, 798, 821
TspGWI ACGGA 3 cut(s) 117, 270, 329
TspRI CASTG 3 cut(s) 85, 341, 532
Vha464I CTTAAG 1 cut(s) 166
VpaK11BI GGWCC 4 cut(s) 316, 451, 611, 623
XapI RAATTY 2 cut(s) 40, 570
XmiI GTMKAC 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.