Rh6AG250900

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0007718
Physical Location & Seq
Reverse (-)
43985486 .. 43986813
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG250900.1

Sequence Viewer

Length: 822 bp
ATGGCAATGGCAGGGTCATTCTGGAAGTCATCGTGTTCGTGTTCATATACGTGTTCATTGGTTAGAGTAATAGGAGTTTCAAGAACAAGTAGTGGTGCAGCAGCTGCTATACTTCACGCATCATCTCTATTTCCACCTCCATCCATGGCCTTAAAAATACAAAGAAAAAGTAGCTTAACCAAGTGGAGGTGGTCTCAACAGCAACCACAATTACCAGAAGACAGGAAGAGCGATGCCTTCTTCTTCATACGGAGCTCTAAATACGGAAGCAATAGTATTAGCACTGATGGTGGAAAGGGATATCTGGAATCTACGGACCAAGAGTTGATGAGCCAGTGCGAAATGGGCACTTTCAAGACGTCAGGGCCGGGGGGTCAGCATCGCAACAAGCGAGAGTCTGCAGTACGCCTTAAGCATCTCCCAACTGGTATTATTGCACAGGCTTCTGAGGATCGATCCCAGCACATGAATCGTGCCTCAGCTTTGTCTCGCCTACGCACTCTTATAGCTCTCAAAGTCAGGAACACTGTGGATCTCGATGGTTATTCCCCTCCTCGAGAGCTTCTTCAAATTCTTCCCAAATCATCTTCCATTAGAACATCAGATATTGGTAACCAAATTGGACCGAACAATCCGAAATTTGTCTACGGAATGCAAGCGCTGTTGGATCTCCTTTATGCAGTTGATGGTTCTGTCTCAGAGGCAGCAAAGTTTCTGGGGTTGAGCACAGGTGCACTGTCTCGATTAATACTCTCTGATGATTCTCTTCGATTGGCAGTCAATGAGCTTAGGGCTTCTAAGGACATGAAGCCTTTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

273

Amino Acids

29.82

Weight (kDa)

9.87

Isoelectric Point (pI)

47.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 111 - 170 3.3e-19 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 362
AccI GTMKAC 1 cut(s) 645
AclWI GGATC 4 cut(s) 450, 459, 540, 675
AcsI RAATTY 2 cut(s) 570, 638
AcyI GRCGYC 1 cut(s) 359
AfaI GTAC 1 cut(s) 405
AfeI AGCGCT 1 cut(s) 660
AfiI CCNNNNNNNGG 1 cut(s) 186
AflII CTTAAG 1 cut(s) 410
AflIII ACRYGT 1 cut(s) 50
AgsI TTSAA 3 cut(s) 81, 355, 569
AluBI AGCT 7 cut(s) 104, 174, 255, 482, 509, 562, 787
AluI AGCT 7 cut(s) 104, 174, 255, 482, 509, 562, 787
Alw21I GWGCWC 3 cut(s) 257, 728, 736
Alw26I GTCTC 4 cut(s) 198, 492, 700, 744
Alw44I GTGCAC 1 cut(s) 732
AlwI GGATC 4 cut(s) 450, 459, 540, 675
AlwNI CAGNNNCTG 1 cut(s) 104
Ama87I CYCGRG 1 cut(s) 555
Aor51HI AGCGCT 1 cut(s) 660
AoxI GGCC 2 cut(s) 147, 365
ApaLI GTGCAC 1 cut(s) 732
ApeKI GCWGC 4 cut(s) 98, 101, 104, 704
ApoI RAATTY 2 cut(s) 570, 638
AseI ATTAAT 1 cut(s) 746
AspLEI GCGC 1 cut(s) 661
AspS9I GGNCC 3 cut(s) 316, 365, 623
AsuC2I CCSGG 1 cut(s) 369
AvaI CYCGRG 1 cut(s) 555
AvaII GGWCC 2 cut(s) 316, 623
BaeGI GKGCMC 2 cut(s) 350, 736
BanII GRGCYC 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 3 cut(s) 257, 728, 736
BbvCI CCTCAGC 1 cut(s) 478
BbvI GCAGC 4 cut(s) 91, 110, 113, 716
BccI CCATC 4 cut(s) 148, 281, 533, 680
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BcnI CCSGG 1 cut(s) 369
BcoDI GTCTC 4 cut(s) 198, 492, 700, 744
BfmI CTRYAG 1 cut(s) 399
BfoI RGCGCY 1 cut(s) 662
BfrI CTTAAG 1 cut(s) 410
BisI GCNGC 4 cut(s) 99, 102, 105, 705
BlsI GCNGC 4 cut(s) 100, 103, 106, 706
Bme1390I CCNGG 1 cut(s) 369
Bme18I GGWCC 2 cut(s) 316, 623
BmeT110I CYCGRG 1 cut(s) 555
BmgT120I GGNCC 3 cut(s) 316, 365, 623
BmrFI CCNGG 1 cut(s) 369
BmsI GCATC 4 cut(s) 128, 223, 388, 424
BpiI GAAGAC 1 cut(s) 225
BplI GAGNNNNNCTC 2 cut(s) 178, 210
Bpu10I CCTNAGC 2 cut(s) 478, 788
BpuMI CCSGG 1 cut(s) 369
Bsa29I ATCGAT 1 cut(s) 454
BsaAI YACGTR 1 cut(s) 51
BsaHI GRCGYC 1 cut(s) 359
BsaI GGTCTC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 144, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse1I ACTGG 2 cut(s) 334, 430
Bse3DI GCAATG 1 cut(s) 12
BseCI ATCGAT 1 cut(s) 454
BseDI CCNNGG 2 cut(s) 144, 368
BseGI GGATG 1 cut(s) 140
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 3 cut(s) 438, 492, 711
BseNI ACTGG 2 cut(s) 334, 430
BseRI GAGGAG 1 cut(s) 543
BseSI GKGCMC 2 cut(s) 350, 736
BseXI GCAGC 4 cut(s) 91, 110, 113, 716
BseYI CCCAGC 1 cut(s) 459
BsgI GTGCAG 1 cut(s) 117
BshFI GGCC 2 cut(s) 149, 367
BshVI ATCGAT 1 cut(s) 454
BsiHKAI GWGCWC 3 cut(s) 257, 728, 736
BsiHKCI CYCGRG 1 cut(s) 555
BsiSI CCGG 1 cut(s) 368
BslI CCNNNNNNNGG 1 cut(s) 186
BsmAI GTCTC 4 cut(s) 198, 492, 700, 744
BsmI GAATGC 1 cut(s) 657
BsnI GGCC 2 cut(s) 149, 367
Bso31I GGTCTC 1 cut(s) 198
BsoBI CYCGRG 1 cut(s) 555
Bsp1286I GDGCHC 4 cut(s) 257, 350, 728, 736
Bsp143I GATC 4 cut(s) 451, 455, 532, 667
Bsp19I CCATGG 1 cut(s) 144
BspANI GGCC 2 cut(s) 149, 367
BspCNI CTCAG 3 cut(s) 439, 491, 710
BspDI ATCGAT 1 cut(s) 454
BspMAI CTGCAG 1 cut(s) 403
BspPI GGATC 4 cut(s) 450, 459, 540, 675
BspQI GCTCTTC 1 cut(s) 221
BspTI CTTAAG 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 2 cut(s) 334, 430
BssECI CCNNGG 2 cut(s) 144, 368
BssMI GATC 4 cut(s) 451, 455, 532, 667
BssNI GRCGYC 1 cut(s) 359
BssT1I CCWWGG 1 cut(s) 144
Bst4CI ACNGT 2 cut(s) 529, 738
Bst6I CTCTTC 2 cut(s) 221, 771
BstACI GRCGYC 1 cut(s) 359
BstAFI CTTAAG 1 cut(s) 410
BstAPI GCANNNNNTGC 1 cut(s) 104
BstBAI YACGTR 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 657
BstDEI CTNAG 5 cut(s) 447, 478, 697, 788, 798
BstDSI CCRYGG 1 cut(s) 144
BstEII GGTNACC 1 cut(s) 611
BstF5I GGATG 1 cut(s) 140
BstH2I RGCGCY 1 cut(s) 662
BstHHI GCGC 1 cut(s) 661
BstKTI GATC 4 cut(s) 454, 458, 535, 670
BstMAI GTCTC 4 cut(s) 198, 492, 700, 744
BstMBI GATC 4 cut(s) 451, 455, 532, 667
BstMWI GCNNNNNNNGC 2 cut(s) 104, 345
BstPI GGTNACC 1 cut(s) 611
BstSCI CCNGG 1 cut(s) 367
BstSFI CTRYAG 1 cut(s) 399
BstSLI GKGCMC 2 cut(s) 350, 736
BstV1I GCAGC 4 cut(s) 91, 110, 113, 716
BstV2I GAAGAC 1 cut(s) 225
BstX2I RGATCY 2 cut(s) 532, 667
BstYI RGATCY 2 cut(s) 532, 667
Bsu15I ATCGAT 1 cut(s) 454
BsuRI GGCC 2 cut(s) 149, 367
BsuTUI ATCGAT 1 cut(s) 454
BtgI CCRYGG 1 cut(s) 144
BtgZI GCGATG 2 cut(s) 246, 365
BtsCI GGATG 1 cut(s) 140
BtsIMutI CAGTG 4 cut(s) 282, 341, 525, 734
Cac8I GCNNGC 1 cut(s) 657
CaiI CAGNNNCTG 1 cut(s) 104
CfoI GCGC 1 cut(s) 661
Cfr13I GGNCC 3 cut(s) 316, 365, 623
ClaI ATCGAT 1 cut(s) 454
Csp6I GTAC 1 cut(s) 404
CviAII CATG 3 cut(s) 145, 466, 805
CviQI GTAC 1 cut(s) 404
DdeI CTNAG 5 cut(s) 447, 478, 697, 788, 798
DpnI GATC 4 cut(s) 453, 457, 534, 669
DpnII GATC 4 cut(s) 451, 455, 532, 667
Eam1104I CTCTTC 2 cut(s) 221, 771
EarI CTCTTC 2 cut(s) 221, 771
Ecl136II GAGCTC 1 cut(s) 255
Eco130I CCWWGG 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 257
Eco31I GGTCTC 1 cut(s) 198
Eco32I GATATC 1 cut(s) 302
Eco47I GGWCC 2 cut(s) 316, 623
Eco47III AGCGCT 1 cut(s) 660
Eco53kI GAGCTC 1 cut(s) 255
Eco88I CYCGRG 1 cut(s) 555
Eco91I GGTNACC 1 cut(s) 611
EcoICRI GAGCTC 1 cut(s) 255
EcoO65I GGTNACC 1 cut(s) 611
EcoRV GATATC 1 cut(s) 302
EcoT14I CCWWGG 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 3 cut(s) 148, 469, 808
FaiI YATR 9 cut(s) 46, 48, 110, 146, 248, 467, 506, 678, 806
FatI CATG 3 cut(s) 144, 465, 804
FblI GTMKAC 1 cut(s) 645
Fnu4HI GCNGC 4 cut(s) 99, 102, 105, 705
FokI GGATG 1 cut(s) 127
FriOI GRGCYC 1 cut(s) 257
Fsp4HI GCNGC 4 cut(s) 99, 102, 105, 705
GlaI GCGC 1 cut(s) 660
GluI GCNGC 4 cut(s) 99, 102, 105, 705
GsaI CCCAGC 1 cut(s) 463
HaeII RGCGCY 1 cut(s) 662
HaeIII GGCC 2 cut(s) 149, 367
HapII CCGG 1 cut(s) 368
HhaI GCGC 1 cut(s) 661
Hin1I GRCGYC 1 cut(s) 359
Hin1II CATG 3 cut(s) 148, 469, 808
Hin6I GCGC 1 cut(s) 659
HinP1I GCGC 1 cut(s) 659
HinfI GANTC 4 cut(s) 308, 395, 469, 761
HpaII CCGG 1 cut(s) 368
Hpy166II GTNNAC 2 cut(s) 646, 734
Hpy188I TCNGA 5 cut(s) 448, 604, 636, 700, 757
Hpy188III TCNNGA 8 cut(s) 22, 81, 305, 355, 520, 536, 557, 741
Hpy8I GTNNAC 2 cut(s) 646, 734
HpyAV CCTTC 1 cut(s) 247
HpyCH4III ACNGT 2 cut(s) 529, 738
HpyCH4IV ACGT 2 cut(s) 50, 359
HpyCH4V TGCA 6 cut(s) 98, 401, 437, 655, 680, 734
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 345
HpyF3I CTNAG 5 cut(s) 447, 478, 697, 788, 798
HpySE526I ACGT 2 cut(s) 50, 359
Hsp92I GRCGYC 1 cut(s) 359
Hsp92II CATG 3 cut(s) 148, 469, 808
HspAI GCGC 1 cut(s) 659
Kzo9I GATC 4 cut(s) 451, 455, 532, 667
LguI GCTCTTC 1 cut(s) 221
LmnI GCTCC 1 cut(s) 252
Lsp1109I GCAGC 4 cut(s) 91, 110, 113, 716
LweI GCATC 4 cut(s) 128, 223, 388, 424
MaeII ACGT 2 cut(s) 50, 359
MaeIII GTNAC 1 cut(s) 611
MalI GATC 4 cut(s) 453, 457, 534, 669
MboI GATC 4 cut(s) 451, 455, 532, 667
MboII GAAGA 8 cut(s) 230, 232, 235, 238, 557, 566, 579, 758
MflI RGATCY 2 cut(s) 532, 667
MhlI GDGCHC 4 cut(s) 257, 350, 728, 736
MluCI AATT 4 cut(s) 209, 570, 618, 638
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 645
MnlI CCTC 8 cut(s) 147, 180, 442, 487, 561, 564, 694, 810
MseI TTAA 4 cut(s) 152, 176, 411, 746
MslI CAYNNNNRTG 1 cut(s) 49
MspA1I CMGCKG 1 cut(s) 104
MspCI CTTAAG 1 cut(s) 410
MspI CCGG 1 cut(s) 368
MspR9I CCNGG 1 cut(s) 369
Mva1269I GAATGC 1 cut(s) 657
MwoI GCNNNNNNNGC 2 cut(s) 104, 345
NciI CCSGG 1 cut(s) 369
NcoI CCATGG 1 cut(s) 144
NdeII GATC 4 cut(s) 451, 455, 532, 667
NlaIII CATG 3 cut(s) 148, 469, 808
PaeR7I CTCGAG 1 cut(s) 555
PciSI GCTCTTC 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 388
PctI GAATGC 1 cut(s) 657
PfeI GAWTC 3 cut(s) 308, 469, 761
PkrI GCNGC 4 cut(s) 100, 103, 106, 706
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
Ppu21I YACGTR 1 cut(s) 51
PshBI ATTAAT 1 cut(s) 746
Psp124BI GAGCTC 1 cut(s) 257
PspEI GGTNACC 1 cut(s) 611
PspFI CCCAGC 1 cut(s) 459
PspPI GGNCC 3 cut(s) 316, 365, 623
PstI CTGCAG 1 cut(s) 403
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 2 cut(s) 532, 667
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 1 cut(s) 405
RsaNI GTAC 1 cut(s) 404
RseI CAYNNNNRTG 1 cut(s) 49
SacI GAGCTC 1 cut(s) 257
SapI GCTCTTC 1 cut(s) 221
SaqAI TTAA 4 cut(s) 152, 176, 411, 746
SatI GCNGC 4 cut(s) 99, 102, 105, 705
Sau3AI GATC 4 cut(s) 451, 455, 532, 667
Sau96I GGNCC 3 cut(s) 316, 365, 623
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 4 cut(s) 257, 350, 728, 736
SfaNI GCATC 4 cut(s) 128, 223, 388, 424
SfcI CTRYAG 1 cut(s) 399
Sfr274I CTCGAG 1 cut(s) 555
SinI GGWCC 2 cut(s) 316, 623
SlaI CTCGAG 1 cut(s) 555
SmiMI CAYNNNNRTG 1 cut(s) 49
SmlI CTYRAG 2 cut(s) 410, 555
SmoI CTYRAG 2 cut(s) 410, 555
Sse9I AATT 4 cut(s) 209, 570, 618, 638
SstI GAGCTC 1 cut(s) 257
StyD4I CCNGG 1 cut(s) 367
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 529, 738
TaiI ACGT 2 cut(s) 53, 362
TaqI TCGA 5 cut(s) 454, 537, 556, 742, 769
TaqII GACCGA 1 cut(s) 640
TasI AATT 4 cut(s) 209, 570, 618, 638
TfiI GAWTC 3 cut(s) 308, 469, 761
Tru1I TTAA 4 cut(s) 152, 176, 411, 746
Tru9I TTAA 4 cut(s) 152, 176, 411, 746
TscAI CASTG 4 cut(s) 289, 341, 532, 741
TseI GCWGC 4 cut(s) 98, 101, 104, 704
TspDTI ATGAA 5 cut(s) 33, 45, 235, 482, 821
TspGWI ACGGA 4 cut(s) 265, 279, 329, 663
TspRI CASTG 4 cut(s) 289, 341, 532, 741
Vha464I CTTAAG 1 cut(s) 410
VneI GTGCAC 1 cut(s) 732
VpaK11BI GGWCC 2 cut(s) 316, 623
VspI ATTAAT 1 cut(s) 746
XapI RAATTY 2 cut(s) 570, 638
XhoI CTCGAG 1 cut(s) 555
XmiI GTMKAC 1 cut(s) 645
ZraI GACGTC 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.