FvH4_2g16920

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007718
Physical Location & Seq
Reverse (-)
14701396 .. 14702759
1364 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g16920.t1

Sequence Viewer

Length: 840 bp
ATGGGAATGAGAATGGCAATGGCAGGATCATTGTTGAAATCATCATGTTCGTGCTCATGGGTTAGAGTGTTAGGAGTTTCAAGAACAAGTAGTGGTGCAGCAGCTGCTATACTTCATGCATCATATCCATTTCCACTTCCAACCGTTGCCTTGAAAATACAAAGAAAAAGTAGCTTAACCAAGTGGAGGTGGTCTCAACAGCAACCACAGTTGCCAGAAGACAGGAAGAGCTATGCCTTCTTCTTCATACGGAGCTCTAAATATGGTAGCAGTAGTATTAGCACTGATGGCGGGAAGGGATATCTGGAATCTACGGACCAAGAGTTGTTGAGCCAGTGTGAAATGGGTACTTTCAAGACGTCAGGGCCGGGGGGTCAGCACCGGAACAAGCGAGAGTCTGCAGTACGTCTCAAGCACCTCCCAACTGGTATCATTGCGCAGGCGTCTGAAGACCGGTCCCAGCACATGAATCGTGCCTCAGCATTGTCTCGCCTGCGCACTCTTATAGCTCTCAGAGTCAGGAACCTTGTGGATCTCGATGGTTATTCCCCTCCCCGTGAGCTTCTTCAAATTCTTCCCAAATCATCTTCCATAAGAACATCAGATATAGGTAACCAAATTGGACCCAACAATGCGAAATTTGTTTTCGGAATGCAAGCTCTGTTGGATCTACTTTATGCAGTTGATGGTTCTGTCTCAGAGGCAGCAAAGTATCTGGGGTTAAGCACAGGTGCCCTCTCTCGGTTAATACTCTCTGATGATTCTCTCCGATTGGCAGTCAATGAGCTTAGGGCTTCTAAGGACATGAAGCCTCTGAGAATTCCTAGTTATGCCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

280

Amino Acids

30.54

Weight (kDa)

10.04

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 112 - 170 7.3e-19 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 362
Acc16I TGCGCA 2 cut(s) 438, 497
AccB1I GGYRCC 1 cut(s) 731
AciI CCGC 1 cut(s) 291
AclWI GGATC 3 cut(s) 34, 540, 675
AcsI RAATTY 3 cut(s) 570, 638, 819
AcuI CTGAAG 1 cut(s) 468
AcyI GRCGYC 2 cut(s) 359, 443
AfaI GTAC 2 cut(s) 349, 405
AfiI CCNNNNNNNGG 2 cut(s) 186, 741
AgeI ACCGGT 1 cut(s) 453
AgsI TTSAA 5 cut(s) 37, 81, 154, 355, 569
AluBI AGCT 8 cut(s) 104, 174, 231, 255, 509, 562, 659, 787
AluI AGCT 8 cut(s) 104, 174, 231, 255, 509, 562, 659, 787
Alw21I GWGCWC 2 cut(s) 56, 257
Alw26I GTCTC 4 cut(s) 198, 413, 492, 700
AlwI GGATC 3 cut(s) 34, 540, 675
AlwNI CAGNNNCTG 1 cut(s) 104
AoxI GGCC 1 cut(s) 365
ApeKI GCWGC 4 cut(s) 98, 101, 104, 704
ApoI RAATTY 3 cut(s) 570, 638, 819
AsiGI ACCGGT 1 cut(s) 453
AspLEI GCGC 2 cut(s) 439, 498
AspS9I GGNCC 4 cut(s) 316, 365, 456, 623
AsuC2I CCSGG 1 cut(s) 369
AvaII GGWCC 3 cut(s) 316, 456, 623
BaeGI GKGCMC 1 cut(s) 736
BanI GGYRCC 1 cut(s) 731
BanII GRGCYC 1 cut(s) 257
BbsI GAAGAC 2 cut(s) 225, 456
Bbv12I GWGCWC 2 cut(s) 56, 257
BbvCI CCTCAGC 1 cut(s) 478
BbvI GCAGC 4 cut(s) 91, 110, 113, 716
BccI CCATC 3 cut(s) 281, 533, 680
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BcnI CCSGG 1 cut(s) 369
BcoDI GTCTC 4 cut(s) 198, 413, 492, 700
BfaI CTAG 1 cut(s) 825
BfmI CTRYAG 1 cut(s) 399
BisI GCNGC 4 cut(s) 99, 102, 105, 705
BlsI GCNGC 4 cut(s) 100, 103, 106, 706
Bme1390I CCNGG 1 cut(s) 369
Bme18I GGWCC 3 cut(s) 316, 456, 623
BmgT120I GGNCC 4 cut(s) 316, 365, 456, 623
BmiI GGNNCC 4 cut(s) 458, 524, 625, 733
BmrFI CCNGG 1 cut(s) 369
BmsI GCATC 1 cut(s) 128
BpiI GAAGAC 2 cut(s) 225, 456
BplI GAGNNNNNCTC 2 cut(s) 178, 210
Bpu10I CCTNAGC 2 cut(s) 478, 788
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 369
BsaHI GRCGYC 2 cut(s) 359, 443
BsaI GGTCTC 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 368
BsaWI WCCGGW 2 cut(s) 381, 453
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 741
Bse118I RCCGGY 1 cut(s) 453
Bse1I ACTGG 2 cut(s) 334, 430
Bse3DI GCAATG 2 cut(s) 24, 432
BseDI CCNNGG 1 cut(s) 368
BseLI CCNNNNNNNGG 2 cut(s) 186, 741
BseMI GCAATG 2 cut(s) 24, 432
BseMII CTCAG 4 cut(s) 492, 526, 711, 806
BseNI ACTGG 2 cut(s) 334, 430
BseSI GKGCMC 1 cut(s) 736
BseXI GCAGC 4 cut(s) 91, 110, 113, 716
BseYI CCCAGC 1 cut(s) 459
BsgI GTGCAG 1 cut(s) 117
BshFI GGCC 1 cut(s) 367
BshNI GGYRCC 1 cut(s) 731
BshTI ACCGGT 1 cut(s) 453
BsiHKAI GWGCWC 2 cut(s) 56, 257
BsiSI CCGG 3 cut(s) 368, 382, 454
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 2 cut(s) 186, 741
BsmAI GTCTC 4 cut(s) 198, 413, 492, 700
BsmBI CGTCTC 1 cut(s) 413
BsmFI GGGAC 1 cut(s) 442
BsmI GAATGC 1 cut(s) 657
BsnI GGCC 1 cut(s) 367
Bso31I GGTCTC 1 cut(s) 198
Bsp1286I GDGCHC 3 cut(s) 56, 257, 736
Bsp143I GATC 3 cut(s) 26, 532, 667
BspACI CCGC 1 cut(s) 291
BspANI GGCC 1 cut(s) 367
BspCNI CTCAG 4 cut(s) 491, 525, 710, 807
BspLI GGNNCC 4 cut(s) 458, 524, 625, 733
BspMAI CTGCAG 1 cut(s) 403
BspPI GGATC 3 cut(s) 34, 540, 675
BspQI GCTCTTC 1 cut(s) 221
BspT107I GGYRCC 1 cut(s) 731
BspTNI GGTCTC 1 cut(s) 198
BsrDI GCAATG 2 cut(s) 24, 432
BsrFI RCCGGY 1 cut(s) 453
BsrI ACTGG 2 cut(s) 334, 430
BssAI RCCGGY 1 cut(s) 453
BssECI CCNNGG 1 cut(s) 368
BssMI GATC 3 cut(s) 26, 532, 667
BssNI GRCGYC 2 cut(s) 359, 443
Bst4CI ACNGT 2 cut(s) 145, 210
Bst6I CTCTTC 1 cut(s) 221
BstACI GRCGYC 2 cut(s) 359, 443
BstAPI GCANNNNNTGC 1 cut(s) 104
BstC8I GCNNGC 3 cut(s) 441, 494, 657
BstDEI CTNAG 6 cut(s) 478, 512, 697, 788, 798, 815
BstEII GGTNACC 1 cut(s) 611
BstHHI GCGC 2 cut(s) 439, 498
BstKTI GATC 3 cut(s) 29, 535, 670
BstMAI GTCTC 4 cut(s) 198, 413, 492, 700
BstMBI GATC 3 cut(s) 26, 532, 667
BstMWI GCNNNNNNNGC 2 cut(s) 104, 288
BstPI GGTNACC 1 cut(s) 611
BstSCI CCNGG 1 cut(s) 367
BstSFI CTRYAG 1 cut(s) 399
BstSLI GKGCMC 1 cut(s) 736
BstV1I GCAGC 4 cut(s) 91, 110, 113, 716
BstV2I GAAGAC 2 cut(s) 225, 456
BstX2I RGATCY 2 cut(s) 532, 667
BstYI RGATCY 2 cut(s) 532, 667
BsuRI GGCC 1 cut(s) 367
BtsIMutI CAGTG 2 cut(s) 282, 341
Cac8I GCNNGC 3 cut(s) 441, 494, 657
CaiI CAGNNNCTG 1 cut(s) 104
CfoI GCGC 2 cut(s) 439, 498
Cfr10I RCCGGY 1 cut(s) 453
Cfr13I GGNCC 4 cut(s) 316, 365, 456, 623
CseI GACGC 1 cut(s) 432
Csp6I GTAC 2 cut(s) 348, 404
CspAI ACCGGT 1 cut(s) 453
CviAII CATG 5 cut(s) 45, 57, 116, 466, 805
CviQI GTAC 2 cut(s) 348, 404
DdeI CTNAG 6 cut(s) 478, 512, 697, 788, 798, 815
DpnI GATC 3 cut(s) 28, 534, 669
DpnII GATC 3 cut(s) 26, 532, 667
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Ecl136II GAGCTC 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 257
Eco31I GGTCTC 1 cut(s) 198
Eco32I GATATC 1 cut(s) 302
Eco47I GGWCC 3 cut(s) 316, 456, 623
Eco53kI GAGCTC 1 cut(s) 255
Eco57I CTGAAG 1 cut(s) 468
Eco91I GGTNACC 1 cut(s) 611
EcoICRI GAGCTC 1 cut(s) 255
EcoO65I GGTNACC 1 cut(s) 611
EcoRI GAATTC 1 cut(s) 819
EcoRV GATATC 1 cut(s) 302
EcoT22I ATGCAT 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 257
Esp3I CGTCTC 1 cut(s) 413
FaeI CATG 5 cut(s) 48, 60, 119, 469, 808
FaqI GGGAC 1 cut(s) 442
FatI CATG 5 cut(s) 44, 56, 115, 465, 804
FauI CCCGC 1 cut(s) 284
Fnu4HI GCNGC 4 cut(s) 99, 102, 105, 705
FriOI GRGCYC 1 cut(s) 257
Fsp4HI GCNGC 4 cut(s) 99, 102, 105, 705
FspBI CTAG 1 cut(s) 825
FspI TGCGCA 2 cut(s) 438, 497
GlaI GCGC 2 cut(s) 438, 497
GluI GCNGC 4 cut(s) 99, 102, 105, 705
GsaI CCCAGC 1 cut(s) 463
HaeIII GGCC 1 cut(s) 367
HapII CCGG 3 cut(s) 368, 382, 454
HgaI GACGC 1 cut(s) 432
HhaI GCGC 2 cut(s) 439, 498
Hin1I GRCGYC 2 cut(s) 359, 443
Hin1II CATG 5 cut(s) 48, 60, 119, 469, 808
Hin6I GCGC 2 cut(s) 437, 496
HinP1I GCGC 2 cut(s) 437, 496
HinfI GANTC 5 cut(s) 308, 395, 469, 516, 761
HpaII CCGG 3 cut(s) 368, 382, 454
Hpy188I TCNGA 8 cut(s) 448, 515, 604, 650, 700, 757, 770, 816
Hpy188III TCNNGA 5 cut(s) 81, 305, 355, 520, 536
HpyAV CCTTC 2 cut(s) 247, 289
HpyCH4III ACNGT 2 cut(s) 145, 210
HpyCH4IV ACGT 2 cut(s) 359, 406
HpyCH4V TGCA 5 cut(s) 98, 119, 401, 655, 680
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 288
HpyF3I CTNAG 6 cut(s) 478, 512, 697, 788, 798, 815
HpySE526I ACGT 2 cut(s) 359, 406
Hsp92I GRCGYC 2 cut(s) 359, 443
Hsp92II CATG 5 cut(s) 48, 60, 119, 469, 808
HspAI GCGC 2 cut(s) 437, 496
Kzo9I GATC 3 cut(s) 26, 532, 667
LguI GCTCTTC 1 cut(s) 221
LmnI GCTCC 1 cut(s) 252
Lsp1109I GCAGC 4 cut(s) 91, 110, 113, 716
LweI GCATC 1 cut(s) 128
MaeI CTAG 1 cut(s) 825
MaeII ACGT 2 cut(s) 359, 406
MaeIII GTNAC 1 cut(s) 611
MalI GATC 3 cut(s) 28, 534, 669
MboI GATC 3 cut(s) 26, 532, 667
MboII GAAGA 8 cut(s) 230, 232, 235, 238, 461, 557, 566, 579
MflI RGATCY 2 cut(s) 532, 667
MhlI GDGCHC 3 cut(s) 56, 257, 736
MluCI AATT 4 cut(s) 570, 618, 638, 819
MlyI GAGTC 2 cut(s) 404, 525
MmeI TCCRAC 2 cut(s) 164, 645
MnlI CCTC 7 cut(s) 180, 428, 487, 561, 694, 746, 822
Mph1103I ATGCAT 1 cut(s) 121
MseI TTAA 3 cut(s) 176, 722, 746
MslI CAYNNNNRTG 1 cut(s) 49
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 3 cut(s) 368, 382, 454
MspR9I CCNGG 1 cut(s) 369
Mva1269I GAATGC 1 cut(s) 657
MwoI GCNNNNNNNGC 2 cut(s) 104, 288
NciI CCSGG 1 cut(s) 369
NdeII GATC 3 cut(s) 26, 532, 667
NlaIII CATG 5 cut(s) 48, 60, 119, 469, 808
NlaIV GGNNCC 4 cut(s) 458, 524, 625, 733
NsbI TGCGCA 2 cut(s) 438, 497
NsiI ATGCAT 1 cut(s) 121
PciSI GCTCTTC 1 cut(s) 221
PctI GAATGC 1 cut(s) 657
PfeI GAWTC 3 cut(s) 308, 469, 761
PinAI ACCGGT 1 cut(s) 453
PkrI GCNGC 4 cut(s) 100, 103, 106, 706
PleI GAGTC 2 cut(s) 403, 524
PpsI GAGTC 2 cut(s) 403, 524
Psp124BI GAGCTC 1 cut(s) 257
PspEI GGTNACC 1 cut(s) 611
PspFI CCCAGC 1 cut(s) 459
PspN4I GGNNCC 4 cut(s) 458, 524, 625, 733
PspPI GGNCC 4 cut(s) 316, 365, 456, 623
PstI CTGCAG 1 cut(s) 403
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 2 cut(s) 532, 667
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 2 cut(s) 349, 405
RsaNI GTAC 2 cut(s) 348, 404
RseI CAYNNNNRTG 1 cut(s) 49
SacI GAGCTC 1 cut(s) 257
SapI GCTCTTC 1 cut(s) 221
SaqAI TTAA 3 cut(s) 176, 722, 746
SatI GCNGC 4 cut(s) 99, 102, 105, 705
Sau3AI GATC 3 cut(s) 26, 532, 667
Sau96I GGNCC 4 cut(s) 316, 365, 456, 623
SchI GAGTC 2 cut(s) 404, 525
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 3 cut(s) 56, 257, 736
SfaNI GCATC 1 cut(s) 128
SfcI CTRYAG 1 cut(s) 399
SinI GGWCC 3 cut(s) 316, 456, 623
SmiMI CAYNNNNRTG 1 cut(s) 49
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 4 cut(s) 570, 618, 638, 819
SsiI CCGC 1 cut(s) 291
SspMI CTAG 1 cut(s) 825
SstI GAGCTC 1 cut(s) 257
StyD4I CCNGG 1 cut(s) 367
TaaI ACNGT 2 cut(s) 145, 210
TaiI ACGT 2 cut(s) 362, 409
TaqI TCGA 1 cut(s) 537
TasI AATT 4 cut(s) 570, 618, 638, 819
TfiI GAWTC 3 cut(s) 308, 469, 761
Tru1I TTAA 3 cut(s) 176, 722, 746
Tru9I TTAA 3 cut(s) 176, 722, 746
TscAI CASTG 2 cut(s) 289, 341
TseI GCWGC 4 cut(s) 98, 101, 104, 704
TspDTI ATGAA 4 cut(s) 104, 235, 482, 821
TspGWI ACGGA 2 cut(s) 265, 329
TspRI CASTG 2 cut(s) 289, 341
VpaK11BI GGWCC 3 cut(s) 316, 456, 623
XapI RAATTY 3 cut(s) 570, 638, 819
XspI CTAG 1 cut(s) 825
ZraI GACGTC 1 cut(s) 360
Zsp2I ATGCAT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.