MD17G1285500.v1.1

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007718
Physical Location & Seq
Forward (+)
34466325 .. 34472947
6623 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1285500.v1.1.491

Sequence Viewer

Length: 816 bp
ATGATAATGGCAGTGGCACTGTCAGGGTGGAAATTGTGGTATTCATATTCATGGGGTTTAAGAGTAGTAGGGGGAGTAGGTGGTGTTTCAAGAAGCAGTGGCAGAGCAGCTGCTGCTGCTGTAACACTAAGAAGAAAGGAAAGCTTCAGCAACTGGGCGTGGTCCCAAAAACATCCACCACCGCCACAATCCTTCTTCTTCGTACGGTGCTCTAAGTATGGAAGCAGCAGCAGCAGTAGCAGCGTCAGTGCTAGTAATAGTAATGGGCCTAATGGCTCCAATGTCGGAAAAAGATATCTGGAATCTACGGACCAAGAGCTGATGAGCCAGTGCCAAATGGACACTTTCAAGACGTCTGGTCCGGGGGGTCAGCACCGTAACAAGCGAGAGTCTGCCGTACGCCTCAAGCACCTCCCCACCGGTATTACTGCGCAGGCTGCGGAGGACCGATCCCAGCATATGAATCGTGCCTCAGCCTTGGCTCGCCTTCGCACTCTTATAGCTCTCAAAGTCAGGAACACCGTCGATCTCGATGCTTATTCCCCTCCTCGAGAGCTTCTTCAAATTCTTCCCCCAAAGTCTACCATCAGAGGCTCAGATTGTGGTCCCCAAATTGGTCCCAACAATCCTAAGTTCATTTTGGGAATGCAAGCTCTGCTGGATCTCATTTTCACAGTTGACGGTTCTGTCTCAGAGACAGCAAAGTTCCTGGGGTTGAGCACAGGTGCTCTGTCGCGGTTAATACTCTCGAATGATTCTCTCCGACTAGCAGTCAATGAACTAAGGTTTTCCAAGGGTATGAAGCCTCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

272

Amino Acids

29.4

Weight (kDa)

10.34

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 109 - 168 1.9e-19 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 686
AatII GACGTC 1 cut(s) 356
Acc16I TGCGCA 1 cut(s) 432
AccI GTMKAC 1 cut(s) 581
AccII CGCG 1 cut(s) 736
AciI CCGC 3 cut(s) 182, 440, 736
AclWI GGATC 2 cut(s) 444, 669
AcsI RAATTY 1 cut(s) 564
AcuI CTGAAG 1 cut(s) 130
AcyI GRCGYC 1 cut(s) 353
AfaI GTAC 2 cut(s) 204, 399
AfiI CCNNNNNNNGG 1 cut(s) 614
AgeI ACCGGT 1 cut(s) 419
AgsI TTSAA 3 cut(s) 90, 349, 563
AhdI GACNNNNNGTC 2 cut(s) 357, 770
AjnI CCWGG 1 cut(s) 708
AluBI AGCT 6 cut(s) 110, 144, 319, 503, 556, 653
AluI AGCT 6 cut(s) 110, 144, 319, 503, 556, 653
Alw21I GWGCWC 3 cut(s) 212, 722, 730
Alw26I GTCTC 2 cut(s) 689, 694
AlwI GGATC 2 cut(s) 444, 669
AlwNI CAGNNNCTG 2 cut(s) 113, 153
Ama87I CYCGRG 1 cut(s) 549
AoxI GGCC 1 cut(s) 266
ApeKI GCWGC 9 cut(s) 107, 110, 113, 116, 225, 228, 231, 240, 437
ApoI RAATTY 1 cut(s) 564
AsiGI ACCGGT 1 cut(s) 419
AspLEI GCGC 1 cut(s) 433
AspS9I GGNCC 7 cut(s) 162, 266, 310, 359, 445, 605, 617
AsuC2I CCSGG 1 cut(s) 363
AvaI CYCGRG 1 cut(s) 549
AvaII GGWCC 6 cut(s) 162, 310, 359, 445, 605, 617
Bbv12I GWGCWC 3 cut(s) 212, 722, 730
BbvCI CCTCAGC 1 cut(s) 472
BbvI GCAGC 9 cut(s) 97, 100, 103, 119, 237, 240, 243, 252, 424
BccI CCATC 1 cut(s) 593
BceAI ACGGC 1 cut(s) 380
BcgI CGANNNNNNTGC 4 cut(s) 446, 480, 515, 549
BciT130I CCWGG 1 cut(s) 710
BcnI CCSGG 1 cut(s) 363
BcoDI GTCTC 2 cut(s) 689, 694
BfaI CTAG 2 cut(s) 252, 767
BisI GCNGC 9 cut(s) 108, 111, 114, 117, 226, 229, 232, 241, 438
BlsI GCNGC 9 cut(s) 109, 112, 115, 118, 227, 230, 233, 242, 439
Bme1390I CCNGG 2 cut(s) 363, 710
Bme18I GGWCC 6 cut(s) 162, 310, 359, 445, 605, 617
BmeRI GACNNNNNGTC 2 cut(s) 357, 770
BmeT110I CYCGRG 1 cut(s) 549
BmgT120I GGNCC 7 cut(s) 162, 266, 310, 359, 445, 605, 617
BmiI GGNNCC 4 cut(s) 164, 277, 607, 619
BmrFI CCNGG 2 cut(s) 363, 710
BmrI ACTGGG 1 cut(s) 163
BmsI GCATC 1 cut(s) 523
BmuI ACTGGG 1 cut(s) 163
Bpu10I CCTNAGC 1 cut(s) 472
BpuEI CTTGAG 2 cut(s) 389, 794
BpuMI CCSGG 1 cut(s) 363
BsaHI GRCGYC 1 cut(s) 353
BsaJI CCNNGG 4 cut(s) 362, 477, 709, 792
BsaWI WCCGGW 1 cut(s) 419
Bsc4I CCNNNNNNNGG 1 cut(s) 614
Bse118I RCCGGY 1 cut(s) 419
Bse1I ACTGG 2 cut(s) 158, 328
BseBI CCWGG 1 cut(s) 710
BseDI CCNNGG 4 cut(s) 362, 477, 709, 792
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 614
BseMII CTCAG 3 cut(s) 486, 609, 705
BseNI ACTGG 2 cut(s) 158, 328
BseRI GAGGAG 1 cut(s) 537
BseXI GCAGC 9 cut(s) 97, 100, 103, 119, 237, 240, 243, 252, 424
BseYI CCCAGC 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 736
BshFI GGCC 1 cut(s) 268
BshTI ACCGGT 1 cut(s) 419
BsiHKAI GWGCWC 3 cut(s) 212, 722, 730
BsiHKCI CYCGRG 1 cut(s) 549
BsiSI CCGG 2 cut(s) 362, 420
BsiWI CGTACG 2 cut(s) 202, 397
BslFI GGGAC 3 cut(s) 148, 591, 603
BslI CCNNNNNNNGG 1 cut(s) 614
BsmAI GTCTC 2 cut(s) 689, 694
BsmFI GGGAC 3 cut(s) 148, 591, 603
BsmI GAATGC 1 cut(s) 651
BsnI GGCC 1 cut(s) 268
BsoBI CYCGRG 1 cut(s) 549
Bsp1286I GDGCHC 3 cut(s) 212, 722, 730
Bsp143I GATC 3 cut(s) 449, 526, 661
BspACI CCGC 3 cut(s) 182, 440, 736
BspANI GGCC 1 cut(s) 268
BspCNI CTCAG 3 cut(s) 485, 608, 704
BspFNI CGCG 1 cut(s) 736
BspLI GGNNCC 4 cut(s) 164, 277, 607, 619
BspPI GGATC 2 cut(s) 444, 669
BsrFI RCCGGY 1 cut(s) 419
BsrI ACTGG 2 cut(s) 158, 328
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 4 cut(s) 362, 477, 709, 792
BssMI GATC 3 cut(s) 449, 526, 661
BssNI GRCGYC 1 cut(s) 353
BssT1I CCWWGG 2 cut(s) 477, 792
Bst2UI CCWGG 1 cut(s) 710
Bst4CI ACNGT 6 cut(s) 21, 207, 377, 523, 676, 683
BstACI GRCGYC 1 cut(s) 353
BstAPI GCANNNNNTGC 2 cut(s) 113, 655
BstC8I GCNNGC 3 cut(s) 435, 484, 651
BstDEI CTNAG 7 cut(s) 128, 213, 472, 595, 630, 691, 782
BstF5I GGATG 1 cut(s) 172
BstFNI CGCG 1 cut(s) 736
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 3 cut(s) 452, 529, 664
BstMAI GTCTC 2 cut(s) 689, 694
BstMBI GATC 3 cut(s) 449, 526, 661
BstMWI GCNNNNNNNGC 7 cut(s) 113, 116, 231, 237, 240, 437, 655
BstNI CCWGG 1 cut(s) 710
BstSCI CCNGG 2 cut(s) 361, 708
BstUI CGCG 1 cut(s) 736
BstV1I GCAGC 9 cut(s) 97, 100, 103, 119, 237, 240, 243, 252, 424
BstX2I RGATCY 1 cut(s) 661
BstYI RGATCY 1 cut(s) 661
BsuRI GGCC 1 cut(s) 268
BtsCI GGATG 1 cut(s) 172
BtsI GCAGTG 2 cut(s) 18, 103
BtsIMutI CAGTG 5 cut(s) 17, 18, 103, 253, 335
Cac8I GCNNGC 3 cut(s) 435, 484, 651
CaiI CAGNNNCTG 2 cut(s) 113, 153
CfoI GCGC 1 cut(s) 433
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 7 cut(s) 162, 266, 310, 359, 445, 605, 617
CseI GACGC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 203, 398
CspAI ACCGGT 1 cut(s) 419
CviAII CATG 1 cut(s) 51
CviQI GTAC 2 cut(s) 203, 398
DdeI CTNAG 7 cut(s) 128, 213, 472, 595, 630, 691, 782
DpnI GATC 3 cut(s) 451, 528, 663
DpnII GATC 3 cut(s) 449, 526, 661
DrdI GACNNNNNNGTC 1 cut(s) 686
DriI GACNNNNNGTC 2 cut(s) 357, 770
DseDI GACNNNNNNGTC 1 cut(s) 686
Eam1105I GACNNNNNGTC 2 cut(s) 357, 770
Eco130I CCWWGG 2 cut(s) 477, 792
Eco32I GATATC 1 cut(s) 296
Eco47I GGWCC 6 cut(s) 162, 310, 359, 445, 605, 617
Eco57I CTGAAG 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 549
EcoRII CCWGG 1 cut(s) 708
EcoRV GATATC 1 cut(s) 296
EcoT14I CCWWGG 2 cut(s) 477, 792
ErhI CCWWGG 2 cut(s) 477, 792
FaeI CATG 1 cut(s) 54
FaiI YATR 7 cut(s) 46, 52, 219, 459, 461, 500, 800
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FaqI GGGAC 3 cut(s) 148, 591, 603
FatI CATG 1 cut(s) 50
FauNDI CATATG 1 cut(s) 459
FblI GTMKAC 1 cut(s) 581
Fnu4HI GCNGC 9 cut(s) 108, 111, 114, 117, 226, 229, 232, 241, 438
FokI GGATG 1 cut(s) 159
Fsp4HI GCNGC 9 cut(s) 108, 111, 114, 117, 226, 229, 232, 241, 438
FspBI CTAG 2 cut(s) 252, 767
FspI TGCGCA 1 cut(s) 432
GlaI GCGC 1 cut(s) 432
GluI GCNGC 9 cut(s) 108, 111, 114, 117, 226, 229, 232, 241, 438
GsaI CCCAGC 1 cut(s) 457
HaeIII GGCC 1 cut(s) 268
HapII CCGG 2 cut(s) 362, 420
HgaI GACGC 1 cut(s) 232
HhaI GCGC 1 cut(s) 433
Hin1I GRCGYC 1 cut(s) 353
Hin1II CATG 1 cut(s) 54
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HindIII AAGCTT 1 cut(s) 142
HinfI GANTC 4 cut(s) 302, 389, 463, 755
HpaII CCGG 2 cut(s) 362, 420
Hpy166II GTNNAC 2 cut(s) 582, 679
Hpy188I TCNGA 5 cut(s) 287, 590, 598, 694, 764
Hpy188III TCNNGA 7 cut(s) 90, 299, 349, 514, 530, 551, 748
Hpy8I GTNNAC 2 cut(s) 582, 679
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 2 cut(s) 202, 497
HpyCH4III ACNGT 6 cut(s) 21, 207, 377, 523, 676, 683
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 1 cut(s) 649
HpyF10VI GCNNNNNNNGC 7 cut(s) 113, 116, 231, 237, 240, 437, 655
HpyF3I CTNAG 7 cut(s) 128, 213, 472, 595, 630, 691, 782
HpySE526I ACGT 1 cut(s) 353
Hsp92I GRCGYC 1 cut(s) 353
Hsp92II CATG 1 cut(s) 54
HspAI GCGC 1 cut(s) 431
Kzo9I GATC 3 cut(s) 449, 526, 661
LmnI GCTCC 1 cut(s) 281
Lsp1109I GCAGC 9 cut(s) 97, 100, 103, 119, 237, 240, 243, 252, 424
LweI GCATC 1 cut(s) 523
MaeI CTAG 2 cut(s) 252, 767
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 2 cut(s) 121, 377
MalI GATC 3 cut(s) 451, 528, 663
MboI GATC 3 cut(s) 449, 526, 661
MboII GAAGA 5 cut(s) 144, 187, 190, 551, 560
MflI RGATCY 1 cut(s) 661
MhlI GDGCHC 3 cut(s) 212, 722, 730
MluCI AATT 3 cut(s) 32, 564, 612
MlyI GAGTC 1 cut(s) 398
MmeI TCCRAC 2 cut(s) 265, 787
MnlI CCTC 8 cut(s) 413, 422, 436, 481, 555, 558, 584, 816
MseI TTAA 2 cut(s) 59, 740
MslI CAYNNNNRTG 1 cut(s) 49
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 2 cut(s) 362, 420
MspR9I CCNGG 2 cut(s) 363, 710
Mva1269I GAATGC 1 cut(s) 651
MvaI CCWGG 1 cut(s) 710
MvnI CGCG 1 cut(s) 736
MwoI GCNNNNNNNGC 7 cut(s) 113, 116, 231, 237, 240, 437, 655
NciI CCSGG 1 cut(s) 363
NdeI CATATG 1 cut(s) 459
NdeII GATC 3 cut(s) 449, 526, 661
NlaIII CATG 1 cut(s) 54
NlaIV GGNNCC 4 cut(s) 164, 277, 607, 619
NsbI TGCGCA 1 cut(s) 432
PaeR7I CTCGAG 1 cut(s) 549
PctI GAATGC 1 cut(s) 651
PfeI GAWTC 3 cut(s) 302, 463, 755
Pfl23II CGTACG 2 cut(s) 202, 397
PinAI ACCGGT 1 cut(s) 419
PkrI GCNGC 9 cut(s) 109, 112, 115, 118, 227, 230, 233, 242, 439
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
Psp6I CCWGG 1 cut(s) 708
PspFI CCCAGC 1 cut(s) 453
PspGI CCWGG 1 cut(s) 708
PspLI CGTACG 2 cut(s) 202, 397
PspN4I GGNNCC 4 cut(s) 164, 277, 607, 619
PspPI GGNCC 7 cut(s) 162, 266, 310, 359, 445, 605, 617
PstNI CAGNNNCTG 2 cut(s) 113, 153
PsuI RGATCY 1 cut(s) 661
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 2 cut(s) 204, 399
RsaNI GTAC 2 cut(s) 203, 398
RseI CAYNNNNRTG 1 cut(s) 49
SaqAI TTAA 2 cut(s) 59, 740
SatI GCNGC 9 cut(s) 108, 111, 114, 117, 226, 229, 232, 241, 438
Sau3AI GATC 3 cut(s) 449, 526, 661
Sau96I GGNCC 7 cut(s) 162, 266, 310, 359, 445, 605, 617
SchI GAGTC 1 cut(s) 398
ScrFI CCNGG 2 cut(s) 363, 710
SduI GDGCHC 3 cut(s) 212, 722, 730
SfaNI GCATC 1 cut(s) 523
Sfr274I CTCGAG 1 cut(s) 549
SinI GGWCC 6 cut(s) 162, 310, 359, 445, 605, 617
SlaI CTCGAG 1 cut(s) 549
SmiMI CAYNNNNRTG 1 cut(s) 49
SmlI CTYRAG 3 cut(s) 404, 549, 809
SmoI CTYRAG 3 cut(s) 404, 549, 809
Sse9I AATT 3 cut(s) 32, 564, 612
SsiI CCGC 3 cut(s) 182, 440, 736
SspMI CTAG 2 cut(s) 252, 767
StyD4I CCNGG 2 cut(s) 361, 708
StyI CCWWGG 2 cut(s) 477, 792
TaaI ACNGT 6 cut(s) 21, 207, 377, 523, 676, 683
TaiI ACGT 1 cut(s) 356
TaqI TCGA 4 cut(s) 525, 531, 550, 749
TaqII GACCGA 1 cut(s) 462
TasI AATT 3 cut(s) 32, 564, 612
TfiI GAWTC 3 cut(s) 302, 463, 755
Tru1I TTAA 2 cut(s) 59, 740
Tru9I TTAA 2 cut(s) 59, 740
TscAI CASTG 5 cut(s) 18, 24, 103, 253, 335
TseI GCWGC 9 cut(s) 107, 110, 113, 116, 225, 228, 231, 240, 437
TspDTI ATGAA 6 cut(s) 33, 39, 476, 625, 792, 815
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 5 cut(s) 18, 24, 103, 253, 335
VpaK11BI GGWCC 6 cut(s) 162, 310, 359, 445, 605, 617
XapI RAATTY 1 cut(s) 564
XhoI CTCGAG 1 cut(s) 549
XmiI GTMKAC 1 cut(s) 581
XspI CTAG 2 cut(s) 252, 767
ZraI GACGTC 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.