Rh6BG253500

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0007718
Physical Location & Seq
Reverse (-)
46586471 .. 46587603
1133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG253500.1

Sequence Viewer

Length: 744 bp
ATGAGAATGGCAATGGCAGGGTCATTCTGGAAGTCATCGTGTTCGTGTTCATATACGTGTTCATTGGTTAGAGTAATAGGAGTTTCAAGAACAAGTAGTGGTGCAGCAGCTGCTATACTTCAGCAACCACAATTACCAGAAGACAGGAAGAGCAATGCCTTCTTCTTCATACGGAGCTCTAAATACGGAAGCAATAGTATTAGCACTGATGGTGGAAAGGGATATCTGGAATCTACGGACCAAGAGTTGATGAGCCAGTGCGAAATGGGCACTTTCAAGACGTCAGGGCCGGGGGGTCAGCATCGCAACAAGCGAGAGTCTGCAGTACGCCTTAAGCATCTCCCAACTGGTATTATTGCACAGGCTTCTGAGGATCGATCCCAGCACATGAATCGTGCCTCAGCTTTGTCTCGCCTACGCACTCTTATAGCTCTCAAAGTCAGGAACACTGTGGATCTCGATGGTTATTCCCCTCCTCGAGAGCTTCTTCAAATTCTTCCCAAATCATCTTCCATTAGAACATCAGATATTGGTAACCAAATTGGACCGAACAATCCGAAATTTGTCTACGGAATGCAAGCTCTGTTGGATCTCCTTTATGCAGTTGATGGTTCTGTCTCAGAGGCAGCAAAGTTTCTGGGGTTGAGCACAGGTGCACTGTCTCGATTAATACTCTCTGATGATTCTCTCCGATTGGCAGTCAATGAGCTTAGGGCTTCTAAGGACATGAAGCCTTTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

247

Amino Acids

26.85

Weight (kDa)

9.72

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 85 - 144 2.7e-19 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 284
AccI GTMKAC 1 cut(s) 567
AclWI GGATC 4 cut(s) 372, 381, 462, 597
AcsI RAATTY 2 cut(s) 492, 560
AcuI CTGAAG 1 cut(s) 104
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 1 cut(s) 327
AflII CTTAAG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 3 cut(s) 87, 277, 491
AluBI AGCT 7 cut(s) 110, 177, 404, 431, 484, 581, 709
AluI AGCT 7 cut(s) 110, 177, 404, 431, 484, 581, 709
Alw21I GWGCWC 3 cut(s) 179, 650, 658
Alw26I GTCTC 3 cut(s) 414, 622, 666
Alw44I GTGCAC 1 cut(s) 654
AlwI GGATC 4 cut(s) 372, 381, 462, 597
AlwNI CAGNNNCTG 1 cut(s) 110
Ama87I CYCGRG 1 cut(s) 477
AoxI GGCC 1 cut(s) 287
ApaLI GTGCAC 1 cut(s) 654
ApeKI GCWGC 4 cut(s) 104, 107, 110, 626
ApoI RAATTY 2 cut(s) 492, 560
AseI ATTAAT 1 cut(s) 668
AspS9I GGNCC 3 cut(s) 238, 287, 545
AsuC2I CCSGG 1 cut(s) 291
AvaI CYCGRG 1 cut(s) 477
AvaII GGWCC 2 cut(s) 238, 545
BaeGI GKGCMC 2 cut(s) 272, 658
BanII GRGCYC 1 cut(s) 179
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 3 cut(s) 179, 650, 658
BbvCI CCTCAGC 1 cut(s) 400
BbvI GCAGC 4 cut(s) 97, 116, 119, 638
BccI CCATC 3 cut(s) 203, 455, 602
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BcnI CCSGG 1 cut(s) 291
BcoDI GTCTC 3 cut(s) 414, 622, 666
BfmI CTRYAG 1 cut(s) 321
BfrI CTTAAG 1 cut(s) 332
BisI GCNGC 4 cut(s) 105, 108, 111, 627
BlsI GCNGC 4 cut(s) 106, 109, 112, 628
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 2 cut(s) 238, 545
BmeT110I CYCGRG 1 cut(s) 477
BmgT120I GGNCC 3 cut(s) 238, 287, 545
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 2 cut(s) 310, 346
BpiI GAAGAC 1 cut(s) 147
Bpu10I CCTNAGC 2 cut(s) 400, 710
BpuMI CCSGG 1 cut(s) 291
Bsa29I ATCGAT 1 cut(s) 376
BsaAI YACGTR 1 cut(s) 57
BsaHI GRCGYC 1 cut(s) 281
BsaJI CCNNGG 1 cut(s) 290
Bse1I ACTGG 2 cut(s) 256, 352
Bse3DI GCAATG 2 cut(s) 18, 160
BseCI ATCGAT 1 cut(s) 376
BseDI CCNNGG 1 cut(s) 290
BseMI GCAATG 2 cut(s) 18, 160
BseMII CTCAG 3 cut(s) 360, 414, 633
BseNI ACTGG 2 cut(s) 256, 352
BseRI GAGGAG 1 cut(s) 465
BseSI GKGCMC 2 cut(s) 272, 658
BseXI GCAGC 4 cut(s) 97, 116, 119, 638
BseYI CCCAGC 1 cut(s) 381
BsgI GTGCAG 1 cut(s) 123
BshFI GGCC 1 cut(s) 289
BshVI ATCGAT 1 cut(s) 376
BsiHKAI GWGCWC 3 cut(s) 179, 650, 658
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 1 cut(s) 290
BsmAI GTCTC 3 cut(s) 414, 622, 666
BsmI GAATGC 1 cut(s) 579
BsnI GGCC 1 cut(s) 289
BsoBI CYCGRG 1 cut(s) 477
Bsp1286I GDGCHC 4 cut(s) 179, 272, 650, 658
Bsp143I GATC 4 cut(s) 373, 377, 454, 589
BspANI GGCC 1 cut(s) 289
BspCNI CTCAG 3 cut(s) 361, 413, 632
BspDI ATCGAT 1 cut(s) 376
BspMAI CTGCAG 1 cut(s) 325
BspPI GGATC 4 cut(s) 372, 381, 462, 597
BspQI GCTCTTC 1 cut(s) 143
BspTI CTTAAG 1 cut(s) 332
BsrDI GCAATG 2 cut(s) 18, 160
BsrI ACTGG 2 cut(s) 256, 352
BssECI CCNNGG 1 cut(s) 290
BssMI GATC 4 cut(s) 373, 377, 454, 589
BssNI GRCGYC 1 cut(s) 281
Bst4CI ACNGT 2 cut(s) 451, 660
Bst6I CTCTTC 1 cut(s) 143
BstACI GRCGYC 1 cut(s) 281
BstAFI CTTAAG 1 cut(s) 332
BstAPI GCANNNNNTGC 1 cut(s) 110
BstBAI YACGTR 1 cut(s) 57
BstC8I GCNNGC 1 cut(s) 579
BstDEI CTNAG 5 cut(s) 369, 400, 619, 710, 720
BstEII GGTNACC 1 cut(s) 533
BstKTI GATC 4 cut(s) 376, 380, 457, 592
BstMAI GTCTC 3 cut(s) 414, 622, 666
BstMBI GATC 4 cut(s) 373, 377, 454, 589
BstMWI GCNNNNNNNGC 2 cut(s) 110, 267
BstPI GGTNACC 1 cut(s) 533
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 1 cut(s) 321
BstSLI GKGCMC 2 cut(s) 272, 658
BstV1I GCAGC 4 cut(s) 97, 116, 119, 638
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 2 cut(s) 454, 589
BstYI RGATCY 2 cut(s) 454, 589
Bsu15I ATCGAT 1 cut(s) 376
BsuRI GGCC 1 cut(s) 289
BsuTUI ATCGAT 1 cut(s) 376
BtgZI GCGATG 1 cut(s) 287
BtsIMutI CAGTG 4 cut(s) 204, 263, 447, 656
Cac8I GCNNGC 1 cut(s) 579
CaiI CAGNNNCTG 1 cut(s) 110
Cfr13I GGNCC 3 cut(s) 238, 287, 545
ClaI ATCGAT 1 cut(s) 376
Csp6I GTAC 1 cut(s) 326
CviAII CATG 2 cut(s) 388, 727
CviQI GTAC 1 cut(s) 326
DdeI CTNAG 5 cut(s) 369, 400, 619, 710, 720
DpnI GATC 4 cut(s) 375, 379, 456, 591
DpnII GATC 4 cut(s) 373, 377, 454, 589
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Ecl136II GAGCTC 1 cut(s) 177
Eco24I GRGCYC 1 cut(s) 179
Eco32I GATATC 1 cut(s) 224
Eco47I GGWCC 2 cut(s) 238, 545
Eco53kI GAGCTC 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 104
Eco88I CYCGRG 1 cut(s) 477
Eco91I GGTNACC 1 cut(s) 533
EcoICRI GAGCTC 1 cut(s) 177
EcoO65I GGTNACC 1 cut(s) 533
EcoRV GATATC 1 cut(s) 224
EcoT38I GRGCYC 1 cut(s) 179
FaeI CATG 2 cut(s) 391, 730
FaiI YATR 8 cut(s) 52, 54, 116, 170, 389, 428, 600, 728
FatI CATG 2 cut(s) 387, 726
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 4 cut(s) 105, 108, 111, 627
FriOI GRGCYC 1 cut(s) 179
Fsp4HI GCNGC 4 cut(s) 105, 108, 111, 627
GluI GCNGC 4 cut(s) 105, 108, 111, 627
GsaI CCCAGC 1 cut(s) 385
HaeIII GGCC 1 cut(s) 289
HapII CCGG 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 281
Hin1II CATG 2 cut(s) 391, 730
HinfI GANTC 4 cut(s) 230, 317, 391, 683
HpaII CCGG 1 cut(s) 290
Hpy166II GTNNAC 2 cut(s) 568, 656
Hpy188I TCNGA 6 cut(s) 370, 526, 558, 622, 679, 692
Hpy188III TCNNGA 8 cut(s) 28, 87, 227, 277, 442, 458, 479, 663
Hpy8I GTNNAC 2 cut(s) 568, 656
HpyAV CCTTC 1 cut(s) 169
HpyCH4III ACNGT 2 cut(s) 451, 660
HpyCH4IV ACGT 2 cut(s) 56, 281
HpyCH4V TGCA 6 cut(s) 104, 323, 359, 577, 602, 656
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 267
HpyF3I CTNAG 5 cut(s) 369, 400, 619, 710, 720
HpySE526I ACGT 2 cut(s) 56, 281
Hsp92I GRCGYC 1 cut(s) 281
Hsp92II CATG 2 cut(s) 391, 730
Kzo9I GATC 4 cut(s) 373, 377, 454, 589
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 1 cut(s) 174
Lsp1109I GCAGC 4 cut(s) 97, 116, 119, 638
LweI GCATC 2 cut(s) 310, 346
MaeII ACGT 2 cut(s) 56, 281
MaeIII GTNAC 1 cut(s) 533
MalI GATC 4 cut(s) 375, 379, 456, 591
MboI GATC 4 cut(s) 373, 377, 454, 589
MboII GAAGA 7 cut(s) 152, 154, 157, 160, 479, 488, 501
MflI RGATCY 2 cut(s) 454, 589
MhlI GDGCHC 4 cut(s) 179, 272, 650, 658
MluCI AATT 4 cut(s) 131, 492, 540, 560
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 1 cut(s) 567
MnlI CCTC 6 cut(s) 364, 409, 483, 486, 616, 732
MseI TTAA 2 cut(s) 333, 668
MslI CAYNNNNRTG 1 cut(s) 55
MspA1I CMGCKG 1 cut(s) 110
MspCI CTTAAG 1 cut(s) 332
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 291
Mva1269I GAATGC 1 cut(s) 579
MwoI GCNNNNNNNGC 2 cut(s) 110, 267
NciI CCSGG 1 cut(s) 291
NdeII GATC 4 cut(s) 373, 377, 454, 589
NlaIII CATG 2 cut(s) 391, 730
PaeR7I CTCGAG 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 310
PctI GAATGC 1 cut(s) 579
PfeI GAWTC 3 cut(s) 230, 391, 683
PkrI GCNGC 4 cut(s) 106, 109, 112, 628
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
Ppu21I YACGTR 1 cut(s) 57
PshBI ATTAAT 1 cut(s) 668
Psp124BI GAGCTC 1 cut(s) 179
PspEI GGTNACC 1 cut(s) 533
PspFI CCCAGC 1 cut(s) 381
PspPI GGNCC 3 cut(s) 238, 287, 545
PstI CTGCAG 1 cut(s) 325
PstNI CAGNNNCTG 1 cut(s) 110
PsuI RGATCY 2 cut(s) 454, 589
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 327
RsaNI GTAC 1 cut(s) 326
RseI CAYNNNNRTG 1 cut(s) 55
SacI GAGCTC 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 143
SaqAI TTAA 2 cut(s) 333, 668
SatI GCNGC 4 cut(s) 105, 108, 111, 627
Sau3AI GATC 4 cut(s) 373, 377, 454, 589
Sau96I GGNCC 3 cut(s) 238, 287, 545
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 4 cut(s) 179, 272, 650, 658
SfaNI GCATC 2 cut(s) 310, 346
SfcI CTRYAG 1 cut(s) 321
Sfr274I CTCGAG 1 cut(s) 477
SinI GGWCC 2 cut(s) 238, 545
SlaI CTCGAG 1 cut(s) 477
SmiMI CAYNNNNRTG 1 cut(s) 55
SmlI CTYRAG 2 cut(s) 332, 477
SmoI CTYRAG 2 cut(s) 332, 477
Sse9I AATT 4 cut(s) 131, 492, 540, 560
SstI GAGCTC 1 cut(s) 179
StyD4I CCNGG 1 cut(s) 289
TaaI ACNGT 2 cut(s) 451, 660
TaiI ACGT 2 cut(s) 59, 284
TaqI TCGA 4 cut(s) 376, 459, 478, 664
TaqII GACCGA 1 cut(s) 562
TasI AATT 4 cut(s) 131, 492, 540, 560
TfiI GAWTC 3 cut(s) 230, 391, 683
Tru1I TTAA 2 cut(s) 333, 668
Tru9I TTAA 2 cut(s) 333, 668
TscAI CASTG 4 cut(s) 211, 263, 454, 663
TseI GCWGC 4 cut(s) 104, 107, 110, 626
TspDTI ATGAA 5 cut(s) 39, 51, 157, 404, 743
TspGWI ACGGA 4 cut(s) 187, 201, 251, 585
TspRI CASTG 4 cut(s) 211, 263, 454, 663
Vha464I CTTAAG 1 cut(s) 332
VneI GTGCAC 1 cut(s) 654
VpaK11BI GGWCC 2 cut(s) 238, 545
VspI ATTAAT 1 cut(s) 668
XapI RAATTY 2 cut(s) 492, 560
XhoI CTCGAG 1 cut(s) 477
XmiI GTMKAC 1 cut(s) 567
ZraI GACGTC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.