AT1G33710

zinc-binding in reverse transcriptase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012717
Physical Location & Seq
Forward (+)
12219657 .. 12220289
633 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G33710.1

Sequence Viewer

Length: 633 bp
ATGGGTAGTTTTTCTATTGATCTACCTACTCTATCCGGGGAGTTGGATGTTTATGATTGGGTGGTGGAAGACAAGCTATGCAACGGCTTTTCCTCTGCGAAAACTTGGGCAGCCCTGAGACCAAGATCGGATGTGGTTAGCTGGGCAAAAACGGTGTGGTTTAAAGGTGCAACTCCTAAACATGCTTTCCATATGTGGGTAACAAATCTTGACCGCCTACCCACTAAAACTAGGCTTGCCTCTTGGGGTATGCAATTACAAACGACTTGCGGGCTTTGCTCTTTGGACATCGAGGACAGAGACCATTTGTTTTTGACTTGTGAATTTGCGTGTTTTCTTTGGCACACTGTTAGTGTGAGATTAGAGCTGCCTGCTTTCAGCTTTGTTGTGTGGAACGACCTCATGGACTGGACTCTTCAACGGAACAGACGCTCGCCGCCAACCCTCCGCAAGCTCATTGTGCAATCTGTGCTCTACGCCATTTGGAAGCAGAGAAACAACTTCCTCCACAATCATGAGACTATTCTGCCGTCAGTGGTGTTTAAGACCATTGACAGGGAAATCAAAAACTCCATTACTGCAAGAAGATTGAAGAAGCGGTTCAGGCGGCTTATGACTCTTTGGTTGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RVT

Protein Analysis

210

Amino Acids

24.7

Weight (kDa)

9.71

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 30 - 114 1.5e-25 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 214, 270, 437, 448, 598, 607
AcsI RAATTY 1 cut(s) 323
AfiI CCNNNNNNNGG 2 cut(s) 196, 555
AgsI TTSAA 2 cut(s) 419, 592
AluBI AGCT 5 cut(s) 76, 141, 367, 381, 454
AluI AGCT 5 cut(s) 76, 141, 367, 381, 454
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 3 cut(s) 112, 294, 512
ApeKI GCWGC 2 cut(s) 110, 367
ApoI RAATTY 1 cut(s) 323
Asp700I GAANNNNTTC 1 cut(s) 599
AsuC2I CCSGG 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 474
BbvI GCAGC 2 cut(s) 122, 354
BceAI ACGGC 2 cut(s) 100, 514
BcnI CCSGG 1 cut(s) 37
BcoDI GTCTC 3 cut(s) 112, 294, 512
BfaI CTAG 1 cut(s) 231
BisI GCNGC 4 cut(s) 111, 368, 437, 608
BlsI GCNGC 4 cut(s) 112, 369, 438, 609
Bme1390I CCNGG 1 cut(s) 37
BmrFI CCNGG 1 cut(s) 37
BpiI GAAGAC 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 37
BsaI GGTCTC 2 cut(s) 112, 294
BsaJI CCNNGG 1 cut(s) 36
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 555
Bse1I ACTGG 1 cut(s) 413
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 2 cut(s) 52, 136
BseLI CCNNNNNNNGG 2 cut(s) 196, 555
BseMII CTCAG 1 cut(s) 107
BseNI ACTGG 1 cut(s) 413
BseXI GCAGC 2 cut(s) 122, 354
BseYI CCCAGC 1 cut(s) 141
BsiHKAI GWGCWC 1 cut(s) 474
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 196, 555
BsmAI GTCTC 3 cut(s) 112, 294, 512
Bso31I GGTCTC 2 cut(s) 112, 294
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 2 cut(s) 19, 125
BspACI CCGC 6 cut(s) 214, 270, 437, 448, 598, 607
BspCNI CTCAG 1 cut(s) 108
BspHI TCATGA 1 cut(s) 514
BspTNI GGTCTC 2 cut(s) 112, 294
BsrI ACTGG 1 cut(s) 413
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 2 cut(s) 19, 125
Bst4CI ACNGT 2 cut(s) 154, 349
Bst6I CTCTTC 1 cut(s) 420
BstAPI GCANNNNNTGC 1 cut(s) 469
BstC8I GCNNGC 5 cut(s) 237, 272, 372, 434, 452
BstDEI CTNAG 1 cut(s) 116
BstF5I GGATG 2 cut(s) 52, 136
BstKTI GATC 2 cut(s) 22, 128
BstMAI GTCTC 3 cut(s) 112, 294, 512
BstMBI GATC 2 cut(s) 19, 125
BstMWI GCNNNNNNNGC 4 cut(s) 276, 460, 469, 604
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 35
BstV1I GCAGC 2 cut(s) 122, 354
BstV2I GAAGAC 1 cut(s) 75
BtsCI GGATG 2 cut(s) 52, 136
BtsIMutI CAGTG 2 cut(s) 345, 540
Cac8I GCNNGC 5 cut(s) 237, 272, 372, 434, 452
CciI TCATGA 1 cut(s) 514
CseI GACGC 1 cut(s) 438
CviAII CATG 3 cut(s) 182, 403, 515
DdeI CTNAG 1 cut(s) 116
DpnI GATC 2 cut(s) 21, 127
DpnII GATC 2 cut(s) 19, 125
DraI TTTAAA 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Eco31I GGTCTC 2 cut(s) 112, 294
FaeI CATG 3 cut(s) 185, 406, 518
FaiI YATR 9 cut(s) 54, 79, 183, 192, 194, 251, 404, 516, 614
FatI CATG 3 cut(s) 181, 402, 514
FauI CCCGC 1 cut(s) 263
FauNDI CATATG 1 cut(s) 192
Fnu4HI GCNGC 4 cut(s) 111, 368, 437, 608
FokI GGATG 2 cut(s) 59, 143
Fsp4HI GCNGC 4 cut(s) 111, 368, 437, 608
FspBI CTAG 1 cut(s) 231
GluI GCNGC 4 cut(s) 111, 368, 437, 608
GsaI CCCAGC 1 cut(s) 145
HapII CCGG 1 cut(s) 36
HgaI GACGC 1 cut(s) 438
Hin1II CATG 3 cut(s) 185, 406, 518
HinfI GANTC 2 cut(s) 412, 616
HpaII CCGG 1 cut(s) 36
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 2 cut(s) 209, 515
HpyCH4III ACNGT 2 cut(s) 154, 349
HpyCH4V TGCA 6 cut(s) 81, 170, 253, 463, 581, 628
HpyF10VI GCNNNNNNNGC 4 cut(s) 276, 460, 469, 604
HpyF3I CTNAG 1 cut(s) 116
Hsp92II CATG 3 cut(s) 185, 406, 518
Kzo9I GATC 2 cut(s) 19, 125
LpnPI CCDG 7 cut(s) 49, 127, 128, 384, 394, 541, 589
Lsp1109I GCAGC 2 cut(s) 122, 354
MaeI CTAG 1 cut(s) 231
MaeIII GTNAC 1 cut(s) 199
MalI GATC 2 cut(s) 21, 127
MboI GATC 2 cut(s) 19, 125
MboII GAAGA 4 cut(s) 80, 407, 597, 604
MhlI GDGCHC 1 cut(s) 474
MluCI AATT 2 cut(s) 254, 323
MlyI GAGTC 2 cut(s) 406, 610
MmeI TCCRAC 1 cut(s) 24
MnlI CCTC 6 cut(s) 103, 250, 286, 410, 455, 515
MroXI GAANNNNTTC 1 cut(s) 599
MseI TTAA 2 cut(s) 162, 543
MslI CAYNNNNRTG 1 cut(s) 513
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 37
MwoI GCNNNNNNNGC 4 cut(s) 276, 460, 469, 604
NciI CCSGG 1 cut(s) 37
NdeI CATATG 1 cut(s) 192
NdeII GATC 2 cut(s) 19, 125
NlaIII CATG 3 cut(s) 185, 406, 518
NspI RCATGY 1 cut(s) 185
PagI TCATGA 1 cut(s) 514
PdmI GAANNNNTTC 1 cut(s) 599
PkrI GCNGC 4 cut(s) 112, 369, 438, 609
PleI GAGTC 2 cut(s) 406, 610
PpsI GAGTC 2 cut(s) 406, 610
PspFI CCCAGC 1 cut(s) 141
RseI CAYNNNNRTG 1 cut(s) 513
SaqAI TTAA 2 cut(s) 162, 543
SatI GCNGC 4 cut(s) 111, 368, 437, 608
Sau3AI GATC 2 cut(s) 19, 125
SchI GAGTC 2 cut(s) 406, 610
ScrFI CCNGG 1 cut(s) 37
SduI GDGCHC 1 cut(s) 474
SetI ASST 8 cut(s) 28, 78, 143, 169, 369, 383, 402, 456
SmiMI CAYNNNNRTG 1 cut(s) 513
Sse9I AATT 2 cut(s) 254, 323
SsiI CCGC 6 cut(s) 214, 270, 437, 448, 598, 607
SspMI CTAG 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 35
TaaI ACNGT 2 cut(s) 154, 349
TaqI TCGA 1 cut(s) 291
TasI AATT 2 cut(s) 254, 323
TauI GCSGC 2 cut(s) 439, 610
Tru1I TTAA 2 cut(s) 162, 543
Tru9I TTAA 2 cut(s) 162, 543
TscAI CASTG 2 cut(s) 352, 540
TseI GCWGC 2 cut(s) 110, 367
TspGWI ACGGA 1 cut(s) 436
TspRI CASTG 2 cut(s) 352, 540
XapI RAATTY 1 cut(s) 323
XceI RCATGY 1 cut(s) 185
XmnI GAANNNNTTC 1 cut(s) 599
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.