AT3G25720

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012717
Physical Location & Seq
Forward (+)
9380234 .. 9381405
1172 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25720.1

Sequence Viewer

Length: 849 bp
ATGTCTCCTTTTTGGGTTTGTGACTACGATACTATCCCGTTTTTGATAGCGGTCTGCTTACCAAAAGCTGAGGGAGGGCTAGGTCTCAGGTGGTTTTCAGAATGGAATACAACCCTCAATCTGAAGCTTGTTTGGCGGTTATTCTCCGGAGGAGGATCTTTATGGGTGGATTGGCATAGGTATCACCATCTTCAGGGTCTTGGGGATGCCTCTGTTTCTAAATTCTGGACCTCTCAAGAATTGGTCTCTGATTCCTGGAATTGGAAATGTCTCTTGCGGCTGAGACCTTTAGCAGAGCGTTTCCTTCGATGTAACATAGGCAATGGCCTTACTGCTAGGTTCTGGACAGACAATTGGACGCCATTTGGCCCCTTACTTATTCACATTGGTGCTGCACGTCCTAATAGGTTGAGGATTCCTATTGAAGCTTCTGTTGCAGACGCTACTCAGGGCATTAGTTGGGGTCTTCCTGTCTCGCCTCTTTGTTGCTTGTGTTCTTGTGCACCCGAGACCAGGGATCACTTACTGTTGGGTTGCAGTTTCAGCAAAGCCCTCTGGAGTTCGGTTCAGTCCAGACTTAGGTTGCACTCACTTATTTTTCTGAATTGGGCTGAACTTCTTTTATGGATTCGTCGTTCTAGCACTTCTGCTCCCTCCACACTGAGGAAGCTTGTTGCTCATTCCGTCGTCTACGCAACTTGGAAGCAGAGAAATAACATGCTACATAACTCGCATCACTTGGATCCTGCTTCAGTCTTCAAGATCATCGATAGGAAAATCATTAACTCCATCAACGCAAGAAGACACAAAAAGAAGTTCCGTAATCTTATGAGTTTTTGGCTCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RVT

Protein Analysis

282

Amino Acids

32.47

Weight (kDa)

10.01

Isoelectric Point (pI)

54.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 152 - 186 7.5e-08 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 690
AccIII TCCGGA 1 cut(s) 146
AciI CCGC 3 cut(s) 50, 136, 277
AclWI GGATC 4 cut(s) 163, 525, 737, 750
AcsI RAATTY 1 cut(s) 221
AcuI CTGAAG 3 cut(s) 143, 176, 735
AcyI GRCGYC 1 cut(s) 359
AfiI CCNNNNNNNGG 5 cut(s) 193, 261, 513, 579, 663
AgsI TTSAA 2 cut(s) 425, 760
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 2 cut(s) 254, 512
AleI CACNNNNGTG 1 cut(s) 387
AloI GAACNNNNNNTCC 2 cut(s) 619, 651
AluBI AGCT 4 cut(s) 68, 127, 428, 670
AluI AGCT 4 cut(s) 68, 127, 428, 670
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 7 cut(s) 9, 89, 250, 275, 277, 478, 503
Alw44I GTGCAC 1 cut(s) 501
AlwI GGATC 4 cut(s) 163, 525, 737, 750
Ama87I CYCGRG 1 cut(s) 506
Aor13HI TCCGGA 1 cut(s) 146
AoxI GGCC 2 cut(s) 325, 367
ApaLI GTGCAC 1 cut(s) 501
ApeKI GCWGC 1 cut(s) 392
ApoI RAATTY 1 cut(s) 221
AspS9I GGNCC 2 cut(s) 228, 368
AsuHPI GGTGA 1 cut(s) 176
AvaI CYCGRG 1 cut(s) 506
AvaII GGWCC 1 cut(s) 228
BaeGI GKGCMC 1 cut(s) 505
BamHI GGATCC 1 cut(s) 742
BbsI GAAGAC 3 cut(s) 458, 748, 808
Bbv12I GWGCWC 1 cut(s) 505
BbvCI CCTCAGC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 379
BccI CCATC 2 cut(s) 195, 797
BciT130I CCWGG 2 cut(s) 256, 514
BcoDI GTCTC 7 cut(s) 9, 89, 250, 275, 277, 478, 503
BfaI CTAG 3 cut(s) 80, 336, 639
BisI GCNGC 2 cut(s) 278, 393
BlsI GCNGC 2 cut(s) 279, 394
Bme1390I CCNGG 2 cut(s) 256, 514
Bme18I GGWCC 1 cut(s) 228
BmeT110I CYCGRG 1 cut(s) 506
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 2 cut(s) 228, 368
BmiI GGNNCC 2 cut(s) 370, 744
BmrFI CCNGG 2 cut(s) 256, 514
BmsI GCATC 2 cut(s) 196, 742
BpiI GAAGAC 3 cut(s) 458, 748, 808
BpmI CTGGAG 1 cut(s) 577
Bpu10I CCTNAGC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 219
Bsa29I ATCGAT 1 cut(s) 768
BsaHI GRCGYC 1 cut(s) 359
BsaI GGTCTC 4 cut(s) 89, 250, 277, 503
BsaJI CCNNGG 1 cut(s) 513
BsaWI WCCGGW 1 cut(s) 146
BsaXI ACNNNNNCTCC 2 cut(s) 634, 664
Bsc4I CCNNNNNNNGG 5 cut(s) 193, 261, 513, 579, 663
Bse3DI GCAATG 1 cut(s) 328
BseAI TCCGGA 1 cut(s) 146
BseBI CCWGG 2 cut(s) 256, 514
BseCI ATCGAT 1 cut(s) 768
BseDI CCNNGG 1 cut(s) 513
BseGI GGATG 1 cut(s) 211
BseLI CCNNNNNNNGG 5 cut(s) 193, 261, 513, 579, 663
BseMI GCAATG 1 cut(s) 328
BseMII CTCAG 5 cut(s) 60, 100, 272, 461, 653
BseRI GAGGAG 1 cut(s) 165
BseSI GKGCMC 1 cut(s) 505
BseXI GCAGC 1 cut(s) 379
BsgI GTGCAG 1 cut(s) 378
BshFI GGCC 2 cut(s) 327, 369
BshVI ATCGAT 1 cut(s) 768
BsiHKAI GWGCWC 1 cut(s) 505
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 1 cut(s) 147
BslI CCNNNNNNNGG 5 cut(s) 193, 261, 513, 579, 663
BsmAI GTCTC 7 cut(s) 9, 89, 250, 275, 277, 478, 503
BsnI GGCC 2 cut(s) 327, 369
Bso31I GGTCTC 4 cut(s) 89, 250, 277, 503
BsoBI CYCGRG 1 cut(s) 506
Bsp1286I GDGCHC 1 cut(s) 505
Bsp13I TCCGGA 1 cut(s) 146
Bsp143I GATC 4 cut(s) 155, 517, 742, 762
BspACI CCGC 3 cut(s) 50, 136, 277
BspANI GGCC 2 cut(s) 327, 369
BspCNI CTCAG 5 cut(s) 61, 99, 273, 460, 654
BspDI ATCGAT 1 cut(s) 768
BspEI TCCGGA 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 370, 744
BspPI GGATC 4 cut(s) 163, 525, 737, 750
BspTNI GGTCTC 4 cut(s) 89, 250, 277, 503
BsrDI GCAATG 1 cut(s) 328
BssECI CCNNGG 1 cut(s) 513
BssMI GATC 4 cut(s) 155, 517, 742, 762
BssNI GRCGYC 1 cut(s) 359
Bst2UI CCWGG 2 cut(s) 256, 514
Bst4CI ACNGT 1 cut(s) 528
BstACI GRCGYC 1 cut(s) 359
BstDEI CTNAG 6 cut(s) 69, 86, 281, 447, 578, 662
BstF5I GGATG 1 cut(s) 211
BstKTI GATC 4 cut(s) 158, 520, 745, 765
BstMAI GTCTC 7 cut(s) 9, 89, 250, 275, 277, 478, 503
BstMBI GATC 4 cut(s) 155, 517, 742, 762
BstMWI GCNNNNNNNGC 3 cut(s) 133, 434, 543
BstNI CCWGG 2 cut(s) 256, 514
BstNSI RCATGY 1 cut(s) 721
BstSCI CCNGG 2 cut(s) 254, 512
BstSLI GKGCMC 1 cut(s) 505
BstV1I GCAGC 1 cut(s) 379
BstV2I GAAGAC 3 cut(s) 458, 748, 808
BstX2I RGATCY 2 cut(s) 155, 742
BstYI RGATCY 2 cut(s) 155, 742
Bsu15I ATCGAT 1 cut(s) 768
BsuRI GGCC 2 cut(s) 327, 369
BsuTUI ATCGAT 1 cut(s) 768
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 1 cut(s) 211
BtsIMutI CAGTG 1 cut(s) 659
Cfr13I GGNCC 2 cut(s) 228, 368
ClaI ATCGAT 1 cut(s) 768
CseI GACGC 2 cut(s) 367, 449
CviAII CATG 1 cut(s) 718
DdeI CTNAG 6 cut(s) 69, 86, 281, 447, 578, 662
DpnI GATC 4 cut(s) 157, 519, 744, 764
DpnII GATC 4 cut(s) 155, 517, 742, 762
Eco31I GGTCTC 4 cut(s) 89, 250, 277, 503
Eco47I GGWCC 1 cut(s) 228
Eco57I CTGAAG 3 cut(s) 143, 176, 735
Eco88I CYCGRG 1 cut(s) 506
EcoRII CCWGG 2 cut(s) 254, 512
FaeI CATG 1 cut(s) 721
FaiI YATR 9 cut(s) 163, 177, 317, 625, 719, 726, 830, 845, 847
FatI CATG 1 cut(s) 717
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 2 cut(s) 278, 393
FokI GGATG 1 cut(s) 218
Fsp4HI GCNGC 2 cut(s) 278, 393
FspBI CTAG 3 cut(s) 80, 336, 639
GluI GCNGC 2 cut(s) 278, 393
GsuI CTGGAG 1 cut(s) 577
HaeIII GGCC 2 cut(s) 327, 369
HapII CCGG 1 cut(s) 147
HgaI GACGC 2 cut(s) 367, 449
Hin1I GRCGYC 1 cut(s) 359
Hin1II CATG 1 cut(s) 721
HindIII AAGCTT 3 cut(s) 125, 426, 668
HinfI GANTC 3 cut(s) 251, 415, 628
HpaII CCGG 1 cut(s) 147
HphI GGTGA 1 cut(s) 176
Hpy166II GTNNAC 2 cut(s) 503, 691
Hpy188I TCNGA 4 cut(s) 100, 123, 250, 603
Hpy188III TCNNGA 7 cut(s) 147, 226, 236, 343, 556, 573, 760
Hpy8I GTNNAC 2 cut(s) 503, 691
Hpy99I CGWCG 2 cut(s) 636, 689
HpyAV CCTTC 1 cut(s) 314
HpyCH4III ACNGT 1 cut(s) 528
HpyCH4IV ACGT 1 cut(s) 397
HpyCH4V TGCA 5 cut(s) 395, 437, 503, 537, 586
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 434, 543
HpyF3I CTNAG 6 cut(s) 69, 86, 281, 447, 578, 662
HpySE526I ACGT 1 cut(s) 397
Hsp92I GRCGYC 1 cut(s) 359
Hsp92II CATG 1 cut(s) 721
Kpn2I TCCGGA 1 cut(s) 146
Kzo9I GATC 4 cut(s) 155, 517, 742, 762
LmnI GCTCC 1 cut(s) 655
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 2 cut(s) 196, 742
MaeI CTAG 3 cut(s) 80, 336, 639
MaeII ACGT 1 cut(s) 397
MaeIII GTNAC 2 cut(s) 20, 311
MalI GATC 4 cut(s) 157, 519, 744, 764
MboI GATC 4 cut(s) 155, 517, 742, 762
MboII GAAGA 4 cut(s) 182, 458, 748, 813
MfeI CAATTG 1 cut(s) 352
MflI RGATCY 2 cut(s) 155, 742
MhlI GDGCHC 1 cut(s) 505
MluCI AATT 5 cut(s) 221, 239, 259, 352, 604
MroI TCCGGA 1 cut(s) 146
MseI TTAA 1 cut(s) 783
MslI CAYNNNNRTG 1 cut(s) 387
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 2 cut(s) 256, 514
MunI CAATTG 1 cut(s) 352
MvaI CCWGG 2 cut(s) 256, 514
MwoI GCNNNNNNNGC 3 cut(s) 133, 434, 543
NdeII GATC 4 cut(s) 155, 517, 742, 762
NlaIII CATG 1 cut(s) 721
NlaIV GGNNCC 2 cut(s) 370, 744
NmuCI GTSAC 1 cut(s) 20
NspI RCATGY 1 cut(s) 721
OliI CACNNNNGTG 1 cut(s) 387
PfeI GAWTC 3 cut(s) 251, 415, 628
PfoI TCCNGGA 1 cut(s) 254
PkrI GCNGC 2 cut(s) 279, 394
Psp6I CCWGG 2 cut(s) 254, 512
PspGI CCWGG 2 cut(s) 254, 512
PspN4I GGNNCC 2 cut(s) 370, 744
PspPI GGNCC 2 cut(s) 228, 368
PsuI RGATCY 2 cut(s) 155, 742
RseI CAYNNNNRTG 1 cut(s) 387
SaqAI TTAA 1 cut(s) 783
SatI GCNGC 2 cut(s) 278, 393
Sau3AI GATC 4 cut(s) 155, 517, 742, 762
Sau96I GGNCC 2 cut(s) 228, 368
ScrFI CCNGG 2 cut(s) 256, 514
SduI GDGCHC 1 cut(s) 505
SfaNI GCATC 2 cut(s) 196, 742
SinI GGWCC 1 cut(s) 228
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 1 cut(s) 234
SmoI CTYRAG 1 cut(s) 234
Sse9I AATT 5 cut(s) 221, 239, 259, 352, 604
SsiI CCGC 3 cut(s) 50, 136, 277
SspMI CTAG 3 cut(s) 80, 336, 639
StyD4I CCNGG 2 cut(s) 254, 512
TaaI ACNGT 1 cut(s) 528
TaiI ACGT 1 cut(s) 400
TaqI TCGA 2 cut(s) 307, 768
TasI AATT 5 cut(s) 221, 239, 259, 352, 604
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 3 cut(s) 251, 415, 628
Tru1I TTAA 1 cut(s) 783
Tru9I TTAA 1 cut(s) 783
TscAI CASTG 1 cut(s) 666
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 1 cut(s) 392
Tsp45I GTSAC 1 cut(s) 20
TspGWI ACGGA 2 cut(s) 673, 809
TspRI CASTG 1 cut(s) 666
VneI GTGCAC 1 cut(s) 501
VpaK11BI GGWCC 1 cut(s) 228
XapI RAATTY 1 cut(s) 221
XceI RCATGY 1 cut(s) 721
XmiI GTMKAC 1 cut(s) 690
XspI CTAG 3 cut(s) 80, 336, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.