Rh7AG432500

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0012717
Physical Location & Seq
Reverse (-)
58159610 .. 58160380
771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG432500.1

Sequence Viewer

Length: 588 bp
ATGAATCCTAATAAGACTTCGGGCCCAGATGGTTTTAACTGTTGCTTCTTTCAAAAAGCTTGGTCGATTATTGGTGAAGATGTTATTGCTGTTGTTAAGGAATTTTTTTCTTCAGGTCTCCTCTTAAAGGAGTTAAATTCCACCATCATTACTCTTGTTCCTAAAGTGGCTAACACCACTACTATGAGTGATTTCAGACCTATATCCTGCTGTAACACTTTATATAAAATTATTGCCAAGTTGTTGGCAAACAGGCTAATGGGCGTTCTTCATCTTATTGTTGGGCCTTCCCAATCTGCCTTCATTCCTGGGCGCAGAATTGGAGACAATATCCTTCTTGCTCAAGAACTCCTTCGTGATTACCATAAAGCTACTGTTCATCCTAGATGTGCTCTTATGGTGGACATTATGAAAGCTTATGACACTGTCGAGTGGGATTTCATTCTTGCGACCCTTGAAGCTTTTAAAATTCCTTCCACTTTGATTAGCTGGATCAAGAGCTGCATTTCTTCCCCGAGATTTTCAGTGGCTGTTAATGGTGAATTGGTAGGTTTCTTTGCTAGCAAGAATCAATATTTTTTCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
DUF4283 Exo_endo_phos RVT_1 zf-RVT

Protein Analysis

195

Amino Acids

21.72

Weight (kDa)

8.75

Isoelectric Point (pI)

29.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 57 - 185 6e-19 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 500
AcsI RAATTY 3 cut(s) 101, 136, 468
AcuI CTGAAG 1 cut(s) 96
AfiI CCNNNNNNNGG 1 cut(s) 127
AgsI TTSAA 2 cut(s) 53, 458
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 6 cut(s) 59, 371, 416, 461, 489, 501
AluI AGCT 6 cut(s) 59, 371, 416, 461, 489, 501
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 2 cut(s) 122, 318
AlwI GGATC 1 cut(s) 500
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 514
AoxI GGCC 2 cut(s) 22, 284
ApaI GGGCCC 1 cut(s) 26
ApeKI GCWGC 1 cut(s) 501
ApoI RAATTY 3 cut(s) 101, 136, 468
Asp700I GAANNNNTTC 1 cut(s) 351
AspLEI GCGC 1 cut(s) 315
AspS9I GGNCC 3 cut(s) 22, 23, 284
AsuHPI GGTGA 2 cut(s) 86, 551
AsuNHI GCTAGC 1 cut(s) 560
AvaI CYCGRG 1 cut(s) 514
BaeGI GKGCMC 1 cut(s) 26
BanII GRGCYC 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 394
BbvI GCAGC 1 cut(s) 488
BccI CCATC 2 cut(s) 23, 152
BciT130I CCWGG 1 cut(s) 309
BcoDI GTCTC 2 cut(s) 122, 318
BfaI CTAG 2 cut(s) 384, 561
BisI GCNGC 1 cut(s) 502
BlsI GCNGC 1 cut(s) 503
Bme1390I CCNGG 1 cut(s) 309
BmeT110I CYCGRG 1 cut(s) 514
BmgT120I GGNCC 3 cut(s) 22, 23, 284
BmiI GGNNCC 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 309
BmtI GCTAGC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 308
Bsc4I CCNNNNNNNGG 1 cut(s) 127
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 308
BseGI GGATG 1 cut(s) 379
BseLI CCNNNNNNNGG 1 cut(s) 127
BseRI GAGGAG 1 cut(s) 110
BseSI GKGCMC 1 cut(s) 26
BseXI GCAGC 1 cut(s) 488
BshFI GGCC 2 cut(s) 24, 286
BsiHKAI GWGCWC 1 cut(s) 394
BsiHKCI CYCGRG 1 cut(s) 514
BslI CCNNNNNNNGG 1 cut(s) 127
BsmAI GTCTC 2 cut(s) 122, 318
BsnI GGCC 2 cut(s) 24, 286
Bso31I GGTCTC 1 cut(s) 122
BsoBI CYCGRG 1 cut(s) 514
Bsp120I GGGCCC 1 cut(s) 22
Bsp1286I GDGCHC 2 cut(s) 26, 394
Bsp143I GATC 1 cut(s) 492
BspANI GGCC 2 cut(s) 24, 286
BspLI GGNNCC 1 cut(s) 24
BspOI GCTAGC 1 cut(s) 564
BspPI GGATC 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 3 cut(s) 41, 376, 427
BstC8I GCNNGC 1 cut(s) 562
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 1 cut(s) 379
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 1 cut(s) 495
BstMAI GTCTC 2 cut(s) 122, 318
BstMBI GATC 1 cut(s) 492
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 307
BstSLI GKGCMC 1 cut(s) 26
BstV1I GCAGC 1 cut(s) 488
BstXI CCANNNNNNTGG 1 cut(s) 244
BsuRI GGCC 2 cut(s) 24, 286
BtsCI GGATG 1 cut(s) 379
BtsIMutI CAGTG 2 cut(s) 423, 531
Cac8I GCNNGC 1 cut(s) 562
CaiI CAGNNNCTG 1 cut(s) 530
CfoI GCGC 1 cut(s) 315
Cfr13I GGNCC 3 cut(s) 22, 23, 284
DpnI GATC 1 cut(s) 494
DpnII GATC 1 cut(s) 492
DraI TTTAAA 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 26
Eco31I GGTCTC 1 cut(s) 122
Eco57I CTGAAG 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 514
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 26
FaiI YATR 8 cut(s) 185, 203, 223, 225, 366, 398, 410, 420
FalI AAGNNNNNCTT 2 cut(s) 336, 368
Fnu4HI GCNGC 1 cut(s) 502
FokI GGATG 1 cut(s) 366
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 1 cut(s) 502
FspBI CTAG 2 cut(s) 384, 561
GlaI GCGC 1 cut(s) 314
GluI GCNGC 1 cut(s) 502
HaeIII GGCC 2 cut(s) 24, 286
HhaI GCGC 1 cut(s) 315
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HindIII AAGCTT 3 cut(s) 57, 414, 459
HinfI GANTC 2 cut(s) 4, 568
HphI GGTGA 2 cut(s) 86, 551
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 1 cut(s) 197
Hpy188III TCNNGA 3 cut(s) 344, 356, 496
Hpy8I GTNNAC 1 cut(s) 403
HpyAV CCTTC 5 cut(s) 297, 310, 344, 362, 483
HpyCH4III ACNGT 3 cut(s) 41, 376, 427
HpyCH4V TGCA 1 cut(s) 504
HspAI GCGC 1 cut(s) 313
Kzo9I GATC 1 cut(s) 492
LpnPI CCDG 7 cut(s) 39, 99, 220, 238, 294, 321, 475
Lsp1109I GCAGC 1 cut(s) 488
MaeI CTAG 2 cut(s) 384, 561
MaeIII GTNAC 1 cut(s) 212
MalI GATC 1 cut(s) 494
MboI GATC 1 cut(s) 492
MboII GAAGA 4 cut(s) 89, 102, 260, 501
MhlI GDGCHC 2 cut(s) 26, 394
MluCI AATT 6 cut(s) 101, 136, 228, 318, 468, 542
MnlI CCTC 1 cut(s) 131
MroXI GAANNNNTTC 1 cut(s) 351
MseI TTAA 6 cut(s) 36, 96, 125, 134, 465, 534
MslI CAYNNNNRTG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 309
NdeII GATC 1 cut(s) 492
NheI GCTAGC 1 cut(s) 560
NlaIV GGNNCC 1 cut(s) 24
PdmI GAANNNNTTC 1 cut(s) 351
PfeI GAWTC 2 cut(s) 4, 568
PflFI GACNNNGTC 1 cut(s) 425
PkrI GCNGC 1 cut(s) 503
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 1 cut(s) 24
PspOMI GGGCCC 1 cut(s) 22
PspPI GGNCC 3 cut(s) 22, 23, 284
PstNI CAGNNNCTG 1 cut(s) 530
PsyI GACNNNGTC 1 cut(s) 425
RseI CAYNNNNRTG 1 cut(s) 182
SaqAI TTAA 6 cut(s) 36, 96, 125, 134, 465, 534
SatI GCNGC 1 cut(s) 502
Sau3AI GATC 1 cut(s) 492
Sau96I GGNCC 3 cut(s) 22, 23, 284
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 2 cut(s) 26, 394
SetI ASST 9 cut(s) 61, 118, 202, 373, 418, 463, 491, 503, 553
SmiMI CAYNNNNRTG 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 342
SmoI CTYRAG 1 cut(s) 342
Sse9I AATT 6 cut(s) 101, 136, 228, 318, 468, 542
SspI AATATT 1 cut(s) 575
SspMI CTAG 2 cut(s) 384, 561
StyD4I CCNGG 1 cut(s) 307
TaaI ACNGT 3 cut(s) 41, 376, 427
TaqI TCGA 2 cut(s) 65, 429
TasI AATT 6 cut(s) 101, 136, 228, 318, 468, 542
TfiI GAWTC 2 cut(s) 4, 568
Tru1I TTAA 6 cut(s) 36, 96, 125, 134, 465, 534
Tru9I TTAA 6 cut(s) 36, 96, 125, 134, 465, 534
TscAI CASTG 2 cut(s) 430, 531
TseI GCWGC 1 cut(s) 501
TspDTI ATGAA 6 cut(s) 17, 260, 292, 368, 425, 430
TspRI CASTG 2 cut(s) 430, 531
Tth111I GACNNNGTC 1 cut(s) 425
XagI CCTNNNNNAGG 1 cut(s) 125
XapI RAATTY 3 cut(s) 101, 136, 468
XmnI GAANNNNTTC 1 cut(s) 351
XspI CTAG 2 cut(s) 384, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.