AT5G28615

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012717
Physical Location & Seq
Forward (+)
10611971 .. 10612501
531 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G28615.1

Sequence Viewer

Length: 450 bp
ATGGCGGCTTGTTCCGGTATTACCGCAAAATTTTGGCACGATGACTGGACGGGTCTTGGTCCCTTAATCGACTTAACCGCCCCTCTTGGACCCCAATTCACTGGTTTGTCTCTTGATGTTGTGGTAAGAGACGTTGTTATTGGTTATACTTGGAGATTCTCCACCTCTAGAAGCAAGAATCATATCATCAACATGCTCAAAAACATTCTACCAAACCCGGAGAACATGATTGAGAGTCAGCATGATGACTCTTATCTTTGGAAGGCTGACCATCATGCTCCCTCAAACATCTTCTCTGCTGCAAAGACTTGGTTAGCTCTCTATACCTTTGCTGCCACAGTTCCTTGGAACAAATCCGTCTGGTTTAAAGGTAACTTCCTGAAACATGCCTTCATTTCTTGGGTGGTGACGTGGAATCGGCTTCATACGCATGACAAGTTGAGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

17.08

Weight (kDa)

9.13

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 98 - 148 1.4e-06 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 24, 78
AcsI RAATTY 1 cut(s) 29
AjiI CACGTC 1 cut(s) 411
AluBI AGCT 1 cut(s) 317
AluI AGCT 1 cut(s) 317
Alw26I GTCTC 2 cut(s) 114, 123
ApeKI GCWGC 2 cut(s) 299, 332
ApoI RAATTY 1 cut(s) 29
AspS9I GGNCC 2 cut(s) 59, 89
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 1 cut(s) 418
AvaII GGWCC 2 cut(s) 59, 89
BbvI GCAGC 2 cut(s) 286, 319
BccI CCATC 1 cut(s) 279
BcnI CCSGG 1 cut(s) 218
BcoDI GTCTC 2 cut(s) 114, 123
BfaI CTAG 1 cut(s) 168
BisI GCNGC 3 cut(s) 6, 300, 333
BlsI GCNGC 3 cut(s) 7, 301, 334
Bme1390I CCNGG 1 cut(s) 218
Bme18I GGWCC 2 cut(s) 59, 89
BmgBI CACGTC 1 cut(s) 411
BmgT120I GGNCC 2 cut(s) 59, 89
BmiI GGNNCC 2 cut(s) 61, 91
BmrFI CCNGG 1 cut(s) 218
BpuMI CCSGG 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 344
BsaWI WCCGGW 1 cut(s) 14
Bse1I ACTGG 2 cut(s) 50, 106
BseDI CCNNGG 1 cut(s) 344
BseNI ACTGG 2 cut(s) 50, 106
BseXI GCAGC 2 cut(s) 286, 319
BsiSI CCGG 2 cut(s) 15, 218
BslFI GGGAC 1 cut(s) 45
BsmAI GTCTC 2 cut(s) 114, 123
BsmBI CGTCTC 1 cut(s) 123
BsmFI GGGAC 1 cut(s) 45
BspACI CCGC 3 cut(s) 5, 24, 78
BspLI GGNNCC 2 cut(s) 61, 91
BsrI ACTGG 2 cut(s) 50, 106
BssECI CCNNGG 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 340
BstMAI GTCTC 2 cut(s) 114, 123
BstMWI GCNNNNNNNGC 1 cut(s) 427
BstNSI RCATGY 2 cut(s) 196, 389
BstSCI CCNGG 1 cut(s) 216
BstV1I GCAGC 2 cut(s) 286, 319
BstXI CCANNNNNNTGG 1 cut(s) 101
BtrI CACGTC 1 cut(s) 411
BtsIMutI CAGTG 1 cut(s) 99
Cfr13I GGNCC 2 cut(s) 59, 89
CviAII CATG 6 cut(s) 193, 226, 242, 275, 386, 431
CviJI RGCY 4 cut(s) 8, 266, 317, 421
CviKI_1 RGCY 4 cut(s) 8, 266, 317, 421
DraI TTTAAA 1 cut(s) 367
Eco130I CCWWGG 1 cut(s) 344
Eco47I GGWCC 2 cut(s) 59, 89
EcoT14I CCWWGG 1 cut(s) 344
ErhI CCWWGG 1 cut(s) 344
Esp3I CGTCTC 1 cut(s) 123
FaeI CATG 6 cut(s) 196, 229, 245, 278, 389, 434
FaqI GGGAC 1 cut(s) 45
FatI CATG 6 cut(s) 192, 225, 241, 274, 385, 430
Fnu4HI GCNGC 3 cut(s) 6, 300, 333
Fsp4HI GCNGC 3 cut(s) 6, 300, 333
FspBI CTAG 1 cut(s) 168
GluI GCNGC 3 cut(s) 6, 300, 333
HapII CCGG 2 cut(s) 15, 218
Hin1II CATG 6 cut(s) 196, 229, 245, 278, 389, 434
HinfI GANTC 5 cut(s) 156, 178, 235, 248, 415
HpaII CCGG 2 cut(s) 15, 218
HphI GGTGA 1 cut(s) 418
Hpy188III TCNNGA 3 cut(s) 113, 168, 379
HpyAV CCTTC 2 cut(s) 256, 400
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4IV ACGT 2 cut(s) 132, 410
HpyCH4V TGCA 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 1 cut(s) 427
HpySE526I ACGT 2 cut(s) 132, 410
Hsp92II CATG 6 cut(s) 196, 229, 245, 278, 389, 434
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 6 cut(s) 28, 31, 87, 231, 346, 392
Lsp1109I GCAGC 2 cut(s) 286, 319
MaeI CTAG 1 cut(s) 168
MaeII ACGT 2 cut(s) 132, 410
MaeIII GTNAC 2 cut(s) 371, 406
MboII GAAGA 1 cut(s) 283
MluCI AATT 2 cut(s) 29, 95
MlyI GAGTC 2 cut(s) 242, 244
MnlI CCTC 4 cut(s) 93, 175, 292, 435
MseI TTAA 3 cut(s) 65, 74, 366
MslI CAYNNNNRTG 2 cut(s) 191, 429
MspI CCGG 2 cut(s) 15, 218
MspR9I CCNGG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 427
NciI CCSGG 1 cut(s) 218
NlaIII CATG 6 cut(s) 196, 229, 245, 278, 389, 434
NlaIV GGNNCC 2 cut(s) 61, 91
NmuCI GTSAC 1 cut(s) 406
NspI RCATGY 2 cut(s) 196, 389
PfeI GAWTC 3 cut(s) 156, 178, 415
PkrI GCNGC 3 cut(s) 7, 301, 334
PleI GAGTC 2 cut(s) 242, 243
PpsI GAGTC 2 cut(s) 242, 243
PspN4I GGNNCC 2 cut(s) 61, 91
PspPI GGNCC 2 cut(s) 59, 89
RseI CAYNNNNRTG 2 cut(s) 191, 429
SaqAI TTAA 3 cut(s) 65, 74, 366
SatI GCNGC 3 cut(s) 6, 300, 333
Sau96I GGNCC 2 cut(s) 59, 89
SchI GAGTC 2 cut(s) 242, 244
ScrFI CCNGG 1 cut(s) 218
SetI ASST 6 cut(s) 135, 167, 319, 329, 373, 413
SinI GGWCC 2 cut(s) 59, 89
SmiMI CAYNNNNRTG 2 cut(s) 191, 429
Sse9I AATT 2 cut(s) 29, 95
SsiI CCGC 3 cut(s) 5, 24, 78
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 216
StyI CCWWGG 1 cut(s) 344
TaaI ACNGT 1 cut(s) 340
TaiI ACGT 2 cut(s) 135, 413
TaqI TCGA 1 cut(s) 69
TasI AATT 2 cut(s) 29, 95
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 156, 178, 415
Tru1I TTAA 3 cut(s) 65, 74, 366
Tru9I TTAA 3 cut(s) 65, 74, 366
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 2 cut(s) 299, 332
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 2 cut(s) 382, 413
TspGWI ACGGA 1 cut(s) 346
TspRI CASTG 1 cut(s) 106
VpaK11BI GGWCC 2 cut(s) 59, 89
XapI RAATTY 1 cut(s) 29
XbaI TCTAGA 1 cut(s) 167
XceI RCATGY 2 cut(s) 196, 389
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.