AT4G05095

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012717
Physical Location & Seq
Reverse (-)
2613798 .. 2614214
417 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G05095.1

Sequence Viewer

Length: 417 bp
ATGAGGAGGTGGGGTCTCTCTATTCCTTCGGATTGTGTCCTTTGCTCTGGAAATGCTGAATTTAGAGAGCACTTGTTCTTTGGATGTGGTGTCAGTTTTGCGGTTTGGGGTAACTTCATGTTTAGAGCAAGCTTAACTCCTCCGCTTCTCTTTATGGACTGCCTCACTTGGGTCTTATCTCCTTCTCGTGATCCTAACATTTCTCTCATCATCAAGCTAGTGTTTCAAGCTTCTATCTACTTCCTTTGGAAAGAGCGTAACCGTAGATTGCATGATCACGCTTCCCGCTCTTCTACTGCAATCATCAAGGAAATCAAGGTCATCCTTACAGCTAAGCTGGATCCTCTCTCTAGGCGTTCTGAATTTGATGCCCCCGACAACTCTCTCCTTTCTACTTGGTTTCGCTACTTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 zf-RVT

Protein Analysis

138

Amino Acids

16.01

Weight (kDa)

9.34

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 1 - 36 1.1e-06 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 288
AciI CCGC 3 cut(s) 101, 143, 286
AclWI GGATC 3 cut(s) 185, 335, 348
AcsI RAATTY 2 cut(s) 59, 362
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 2 cut(s) 227, 412
AluBI AGCT 5 cut(s) 132, 217, 230, 332, 337
AluI AGCT 5 cut(s) 132, 217, 230, 332, 337
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 3 cut(s) 185, 335, 348
ApoI RAATTY 2 cut(s) 59, 362
BamHI GGATCC 1 cut(s) 340
BauI CACGAG 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 72
BclI TGATCA 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 2 cut(s) 218, 351
BlpI GCTNAGC 1 cut(s) 333
BmiI GGNNCC 1 cut(s) 342
BmsI GCATC 1 cut(s) 358
Bpu1102I GCTNAGC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 20
BsaXI ACNNNNNCTCC 1 cut(s) 28
Bsc4I CCNNNNNNNGG 1 cut(s) 169
BseGI GGATG 2 cut(s) 89, 321
BseLI CCNNNNNNNGG 1 cut(s) 169
BseRI GAGGAG 2 cut(s) 19, 129
BsiHKAI GWGCWC 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 169
BsmAI GTCTC 1 cut(s) 20
Bso31I GGTCTC 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 3 cut(s) 190, 274, 340
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 3 cut(s) 101, 143, 286
BspLI GGNNCC 1 cut(s) 342
BspPI GGATC 3 cut(s) 185, 335, 348
BspQI GCTCTTC 1 cut(s) 295
BspTNI GGTCTC 1 cut(s) 20
BsrBI CCGCTC 1 cut(s) 288
BssMI GATC 3 cut(s) 190, 274, 340
BssSI CACGAG 1 cut(s) 186
Bst2BI CACGAG 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 263
Bst6I CTCTTC 1 cut(s) 295
BstC8I GCNNGC 1 cut(s) 130
BstDEI CTNAG 1 cut(s) 333
BstF5I GGATG 2 cut(s) 89, 321
BstKTI GATC 3 cut(s) 193, 277, 343
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 3 cut(s) 190, 274, 340
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BtsCI GGATG 2 cut(s) 89, 321
Cac8I GCNNGC 1 cut(s) 130
CviAII CATG 2 cut(s) 118, 272
CviJI RGCY 5 cut(s) 132, 217, 230, 332, 337
CviKI_1 RGCY 5 cut(s) 132, 217, 230, 332, 337
DdeI CTNAG 1 cut(s) 333
DpnI GATC 3 cut(s) 192, 276, 342
DpnII GATC 3 cut(s) 190, 274, 340
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
Eco31I GGTCTC 1 cut(s) 20
FaeI CATG 2 cut(s) 121, 275
FaiI YATR 3 cut(s) 119, 155, 273
FatI CATG 2 cut(s) 117, 271
FauI CCCGC 1 cut(s) 293
FbaI TGATCA 1 cut(s) 274
FokI GGATG 2 cut(s) 96, 308
FspBI CTAG 2 cut(s) 218, 351
Hin1II CATG 2 cut(s) 121, 275
HindIII AAGCTT 2 cut(s) 130, 228
Hpy188I TCNGA 2 cut(s) 31, 361
Hpy188III TCNNGA 2 cut(s) 48, 188
HpyAV CCTTC 2 cut(s) 36, 192
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4V TGCA 2 cut(s) 271, 299
HpyF3I CTNAG 1 cut(s) 333
Hsp92II CATG 2 cut(s) 121, 275
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 3 cut(s) 190, 274, 340
LguI GCTCTTC 1 cut(s) 295
LpnPI CCDG 2 cut(s) 33, 323
LweI GCATC 1 cut(s) 358
MaeI CTAG 2 cut(s) 218, 351
MaeIII GTNAC 2 cut(s) 110, 257
MalI GATC 3 cut(s) 192, 276, 342
MbiI CCGCTC 1 cut(s) 288
MboI GATC 3 cut(s) 190, 274, 340
MboII GAAGA 1 cut(s) 282
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 2 cut(s) 59, 362
MnlI CCTC 3 cut(s) 150, 173, 354
MseI TTAA 1 cut(s) 134
NdeII GATC 3 cut(s) 190, 274, 340
NlaIII CATG 2 cut(s) 121, 275
NlaIV GGNNCC 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 342
PsuI RGATCY 1 cut(s) 340
SapI GCTCTTC 1 cut(s) 295
SaqAI TTAA 1 cut(s) 134
Sau3AI GATC 3 cut(s) 190, 274, 340
SduI GDGCHC 1 cut(s) 72
SetI ASST 7 cut(s) 11, 134, 219, 232, 321, 334, 339
SfaNI GCATC 1 cut(s) 358
Sse9I AATT 2 cut(s) 59, 362
SsiI CCGC 3 cut(s) 101, 143, 286
SspMI CTAG 2 cut(s) 218, 351
TaaI ACNGT 1 cut(s) 263
TasI AATT 2 cut(s) 59, 362
Tru1I TTAA 1 cut(s) 134
Tru9I TTAA 1 cut(s) 134
TspDTI ATGAA 1 cut(s) 106
XapI RAATTY 2 cut(s) 59, 362
XspI CTAG 2 cut(s) 218, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.