AT4G04650

zinc-binding in reverse transcriptase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012717
Physical Location & Seq
Reverse (-)
2353989 .. 2355213
1225 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G04650.1

Sequence Viewer

Length: 999 bp
ATGTGTAAGCTCAGAGTTGTGGCAAGGCCCTTTATTGTTTGTGAGGTTGGTTCCGGGGTCACCGCTAAGTTTTGGCATGATAATTGGATTGGCCTTGGCCCCCTTATAGAGGTAATCGGTCCTCTAGGCCCTCGTACAGTTGGTTTGCCTATTGATGCTGTGGTTAGGGATGCGTTAAGGGGCACGTCTTGGTGGATTGCATCAAGCCGTAGCAGGAATCCAATCATTGTTCAGCTTAAAAATCTTCTCCCGGAGGCTCAGGGTTTGCTTGACTGTCAGCATGACGACTCCTTTCTTTGGAAAACGGACCTTCATGCACCATCAAACAGATTCTCCGCTCCAAGAACTTGGTCTGCTCTGCATCCGCAATCTCACACCGTCCCCTGGCATAAGGCGGTCTGGTTTAAGAACCATGTTCCTAAGCACGCCTTCATTTGTTGGGTTGTGGCGTGGAATAGATTGCACACAAGGGATAGGCTTCAGAATTGGGGTCTCTCGATCCCTGCTGAGTGTCTTCTTTGCAATGCTCATGACGATTCGAGAGCACATCTCTTCTTTGAGTGCCAGTTTAGTGGTGTCGTGTGGAGGTTTTTTACAGCCTCAACCAACCTTAATCCTCCTGCCCAATTAATGGACTGTCTTAACTGGCTCTTATCTCCCTCAAGGGAAAAAAATATTTGTCTCATTATCAGGCTGGCCTTTCACTCTTGTGTTTATGCAATTTGGAGAGAAAGAAACCAACGTCTCCACTCCGGCGTCTCTCGTTCTACTGAGTCTATCTTGAAGGACATCCAGCTCATCATTCGCGCTCGTCTGGATCCTTTGTCTCGCAGCACGGCTCATCAACCTAACGCTTTGTCCTTGCTAGGAACTTGTGAACCAGAAATAACCGTCTATAAAGCACCCGAAATTGAAGTTAAATTGGATAACTTTAATGAAAAGACTGATATCTATAGTTTCGGGATTGTTTTTTTGAGTTTGGCGCTATTTTGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RVT

Protein Analysis

332

Amino Acids

37.94

Weight (kDa)

8.9

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 111 - 195 5.1e-24 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 631
AccBSI CCGCTC 1 cut(s) 338
AccII CGCG 1 cut(s) 807
AciI CCGC 4 cut(s) 63, 336, 365, 395
AclWI GGATC 3 cut(s) 493, 812, 825
AcuI CTGAAG 1 cut(s) 464
AcyI GRCGYC 1 cut(s) 756
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 4 cut(s) 109, 297, 384, 631
AgsI TTSAA 2 cut(s) 784, 914
AjiI CACGTC 1 cut(s) 186
AjnI CCWGG 1 cut(s) 383
AleI CACNNNNGTG 1 cut(s) 708
AluBI AGCT 3 cut(s) 10, 235, 796
AluI AGCT 3 cut(s) 10, 235, 796
Alw21I GWGCWC 1 cut(s) 547
Alw26I GTCTC 5 cut(s) 497, 686, 749, 763, 831
AlwI GGATC 3 cut(s) 493, 812, 825
AoxI GGCC 5 cut(s) 26, 91, 97, 127, 696
ApeKI GCWGC 1 cut(s) 831
AseI ATTAAT 1 cut(s) 629
AspLEI GCGC 2 cut(s) 809, 985
AspS9I GGNCC 5 cut(s) 27, 98, 119, 128, 307
AsuC2I CCSGG 2 cut(s) 55, 251
AsuHPI GGTGA 1 cut(s) 52
AvaII GGWCC 2 cut(s) 119, 307
BaeGI GKGCMC 1 cut(s) 185
BamHI GGATCC 1 cut(s) 817
BbsI GAAGAC 1 cut(s) 506
Bbv12I GWGCWC 1 cut(s) 547
BbvI GCAGC 1 cut(s) 843
BccI CCATC 1 cut(s) 328
BceAI ACGGC 2 cut(s) 192, 852
BciT130I CCWGG 1 cut(s) 385
BcnI CCSGG 2 cut(s) 55, 251
BcoDI GTCTC 5 cut(s) 497, 686, 749, 763, 831
BfaI CTAG 2 cut(s) 125, 866
BfmI CTRYAG 1 cut(s) 952
BfoI RGCGCY 1 cut(s) 986
BisI GCNGC 1 cut(s) 832
BlsI GCNGC 1 cut(s) 833
Bme1390I CCNGG 3 cut(s) 55, 251, 385
Bme18I GGWCC 2 cut(s) 119, 307
BmgBI CACGTC 1 cut(s) 186
BmgT120I GGNCC 5 cut(s) 27, 98, 119, 128, 307
BmiI GGNNCC 3 cut(s) 52, 100, 819
BmrFI CCNGG 3 cut(s) 55, 251, 385
BmsI GCATC 4 cut(s) 145, 160, 209, 370
BpiI GAAGAC 1 cut(s) 506
BplI GAGNNNNNCTC 2 cut(s) 534, 566
Bpu10I CCTNAGC 2 cut(s) 258, 420
BpuEI CTTGAG 1 cut(s) 646
BpuMI CCSGG 2 cut(s) 55, 251
BsaHI GRCGYC 1 cut(s) 756
BsaI GGTCTC 1 cut(s) 497
BsaJI CCNNGG 3 cut(s) 54, 94, 383
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 297, 384, 631
Bse1I ACTGG 2 cut(s) 565, 650
Bse3DI GCAATG 1 cut(s) 529
BseBI CCWGG 1 cut(s) 385
BseDI CCNNGG 3 cut(s) 54, 94, 383
BseGI GGATG 3 cut(s) 175, 361, 789
BseLI CCNNNNNNNGG 4 cut(s) 109, 297, 384, 631
BseMI GCAATG 1 cut(s) 529
BseMII CTCAG 4 cut(s) 25, 272, 498, 762
BseNI ACTGG 2 cut(s) 565, 650
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 1 cut(s) 843
Bsh1236I CGCG 1 cut(s) 807
BshFI GGCC 5 cut(s) 28, 93, 99, 129, 698
BsiHKAI GWGCWC 1 cut(s) 547
BsiSI CCGG 3 cut(s) 54, 251, 753
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 4 cut(s) 109, 297, 384, 631
BsmAI GTCTC 5 cut(s) 497, 686, 749, 763, 831
BsmBI CGTCTC 2 cut(s) 749, 763
BsmFI GGGAC 1 cut(s) 365
BsnI GGCC 5 cut(s) 28, 93, 99, 129, 698
Bso31I GGTCTC 1 cut(s) 497
Bsp1286I GDGCHC 2 cut(s) 185, 547
Bsp143I GATC 3 cut(s) 498, 817, 993
BspACI CCGC 4 cut(s) 63, 336, 365, 395
BspANI GGCC 5 cut(s) 28, 93, 99, 129, 698
BspCNI CTCAG 4 cut(s) 24, 271, 499, 763
BspFNI CGCG 1 cut(s) 807
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 3 cut(s) 52, 100, 819
BspPI GGATC 3 cut(s) 493, 812, 825
BspTNI GGTCTC 1 cut(s) 497
BsrBI CCGCTC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 529
BsrI ACTGG 2 cut(s) 565, 650
BssECI CCNNGG 3 cut(s) 54, 94, 383
BssMI GATC 3 cut(s) 498, 817, 993
BssNI GRCGYC 1 cut(s) 756
BssT1I CCWWGG 1 cut(s) 94
Bst2UI CCWGG 1 cut(s) 385
Bst4CI ACNGT 5 cut(s) 139, 275, 379, 638, 892
Bst6I CTCTTC 1 cut(s) 557
BstACI GRCGYC 1 cut(s) 756
BstC8I GCNNGC 2 cut(s) 426, 696
BstDEI CTNAG 6 cut(s) 11, 66, 258, 420, 507, 771
BstEII GGTNACC 1 cut(s) 58
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 3 cut(s) 175, 361, 789
BstFNI CGCG 1 cut(s) 807
BstH2I RGCGCY 1 cut(s) 986
BstHHI GCGC 2 cut(s) 809, 985
BstKTI GATC 3 cut(s) 501, 820, 996
BstMAI GTCTC 5 cut(s) 497, 686, 749, 763, 831
BstMBI GATC 3 cut(s) 498, 817, 993
BstNI CCWGG 1 cut(s) 385
BstPI GGTNACC 1 cut(s) 58
BstSCI CCNGG 3 cut(s) 53, 249, 383
BstSFI CTRYAG 1 cut(s) 952
BstSLI GKGCMC 1 cut(s) 185
BstUI CGCG 1 cut(s) 807
BstV1I GCAGC 1 cut(s) 843
BstV2I GAAGAC 1 cut(s) 506
BstX2I RGATCY 2 cut(s) 817, 993
BstXI CCANNNNNNTGG 2 cut(s) 348, 572
BstYI RGATCY 2 cut(s) 817, 993
BsuRI GGCC 5 cut(s) 28, 93, 99, 129, 698
BtrI CACGTC 1 cut(s) 186
BtsCI GGATG 3 cut(s) 175, 361, 789
Cac8I GCNNGC 2 cut(s) 426, 696
CciI TCATGA 1 cut(s) 529
CfoI GCGC 2 cut(s) 809, 985
Cfr13I GGNCC 5 cut(s) 27, 98, 119, 128, 307
CseI GACGC 1 cut(s) 745
Csp6I GTAC 1 cut(s) 135
CviAII CATG 5 cut(s) 77, 281, 314, 413, 530
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 6 cut(s) 11, 66, 258, 420, 507, 771
DpnI GATC 3 cut(s) 500, 819, 995
DpnII GATC 3 cut(s) 498, 817, 993
Eam1104I CTCTTC 1 cut(s) 557
EarI CTCTTC 1 cut(s) 557
Eco130I CCWWGG 1 cut(s) 94
Eco31I GGTCTC 1 cut(s) 497
Eco32I GATATC 1 cut(s) 949
Eco47I GGWCC 2 cut(s) 119, 307
Eco57I CTGAAG 1 cut(s) 464
Eco91I GGTNACC 1 cut(s) 58
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoO109I RGGNCCY 2 cut(s) 27, 128
EcoO65I GGTNACC 1 cut(s) 58
EcoRII CCWGG 1 cut(s) 383
EcoRV GATATC 1 cut(s) 949
EcoT14I CCWWGG 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 94
Esp3I CGTCTC 2 cut(s) 749, 763
FaeI CATG 5 cut(s) 80, 284, 317, 416, 533
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FaqI GGGAC 1 cut(s) 365
FatI CATG 5 cut(s) 76, 280, 313, 412, 529
Fnu4HI GCNGC 1 cut(s) 832
FokI GGATG 3 cut(s) 182, 348, 776
Fsp4HI GCNGC 1 cut(s) 832
FspBI CTAG 2 cut(s) 125, 866
GlaI GCGC 2 cut(s) 808, 984
GluI GCNGC 1 cut(s) 832
HaeII RGCGCY 1 cut(s) 986
HaeIII GGCC 5 cut(s) 28, 93, 99, 129, 698
HapII CCGG 3 cut(s) 54, 251, 753
HgaI GACGC 1 cut(s) 745
HhaI GCGC 2 cut(s) 809, 985
Hin1I GRCGYC 1 cut(s) 756
Hin1II CATG 5 cut(s) 80, 284, 317, 416, 533
Hin6I GCGC 2 cut(s) 807, 983
HinP1I GCGC 2 cut(s) 807, 983
HinfI GANTC 5 cut(s) 217, 287, 330, 536, 773
HpaII CCGG 3 cut(s) 54, 251, 753
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 1 cut(s) 878
Hpy188I TCNGA 3 cut(s) 14, 483, 998
Hpy188III TCNNGA 6 cut(s) 496, 530, 540, 781, 815, 961
Hpy8I GTNNAC 1 cut(s) 878
HpyAV CCTTC 3 cut(s) 320, 439, 778
HpyCH4III ACNGT 5 cut(s) 139, 275, 379, 638, 892
HpyCH4IV ACGT 2 cut(s) 185, 742
HpyCH4V TGCA 6 cut(s) 200, 317, 361, 463, 522, 719
HpyF3I CTNAG 6 cut(s) 11, 66, 258, 420, 507, 771
HpySE526I ACGT 2 cut(s) 185, 742
Hsp92I GRCGYC 1 cut(s) 756
Hsp92II CATG 5 cut(s) 80, 284, 317, 416, 533
HspAI GCGC 2 cut(s) 807, 983
Kzo9I GATC 3 cut(s) 498, 817, 993
LmnI GCTCC 1 cut(s) 343
Lsp1109I GCAGC 1 cut(s) 843
LweI GCATC 4 cut(s) 145, 160, 209, 370
MaeI CTAG 2 cut(s) 125, 866
MaeII ACGT 2 cut(s) 185, 742
MaeIII GTNAC 1 cut(s) 58
MalI GATC 3 cut(s) 500, 819, 995
MbiI CCGCTC 1 cut(s) 338
MboI GATC 3 cut(s) 498, 817, 993
MboII GAAGA 3 cut(s) 236, 506, 544
MflI RGATCY 2 cut(s) 817, 993
MhlI GDGCHC 2 cut(s) 185, 547
MluCI AATT 6 cut(s) 82, 484, 626, 720, 909, 920
MlyI GAGTC 2 cut(s) 281, 782
MnlI CCTC 9 cut(s) 37, 103, 132, 141, 247, 579, 610, 627, 670
MseI TTAA 8 cut(s) 176, 237, 405, 612, 629, 642, 918, 933
MslI CAYNNNNRTG 1 cut(s) 708
MspI CCGG 3 cut(s) 54, 251, 753
MspR9I CCNGG 3 cut(s) 55, 251, 385
MvaI CCWGG 1 cut(s) 385
MvnI CGCG 1 cut(s) 807
NciI CCSGG 2 cut(s) 55, 251
NdeII GATC 3 cut(s) 498, 817, 993
NlaIII CATG 5 cut(s) 80, 284, 317, 416, 533
NlaIV GGNNCC 3 cut(s) 52, 100, 819
NmuCI GTSAC 1 cut(s) 58
OliI CACNNNNGTG 1 cut(s) 708
PagI TCATGA 1 cut(s) 529
PfeI GAWTC 3 cut(s) 217, 330, 536
PflMI CCANNNNNTGG 1 cut(s) 631
PfoI TCCNGGA 1 cut(s) 249
PkrI GCNGC 1 cut(s) 833
PleI GAGTC 2 cut(s) 281, 781
PpsI GAGTC 2 cut(s) 281, 781
PshBI ATTAAT 1 cut(s) 629
Psp6I CCWGG 1 cut(s) 383
PspEI GGTNACC 1 cut(s) 58
PspGI CCWGG 1 cut(s) 383
PspN4I GGNNCC 3 cut(s) 52, 100, 819
PspPI GGNCC 5 cut(s) 27, 98, 119, 128, 307
PsuI RGATCY 2 cut(s) 817, 993
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 1 cut(s) 708
SaqAI TTAA 8 cut(s) 176, 237, 405, 612, 629, 642, 918, 933
SatI GCNGC 1 cut(s) 832
Sau3AI GATC 3 cut(s) 498, 817, 993
Sau96I GGNCC 5 cut(s) 27, 98, 119, 128, 307
SchI GAGTC 2 cut(s) 281, 782
ScrFI CCNGG 3 cut(s) 55, 251, 385
SduI GDGCHC 2 cut(s) 185, 547
SfaNI GCATC 4 cut(s) 145, 160, 209, 370
SfcI CTRYAG 1 cut(s) 952
SinI GGWCC 2 cut(s) 119, 307
SmiMI CAYNNNNRTG 1 cut(s) 708
SmlI CTYRAG 1 cut(s) 661
SmoI CTYRAG 1 cut(s) 661
Sse9I AATT 6 cut(s) 82, 484, 626, 720, 909, 920
SsiI CCGC 4 cut(s) 63, 336, 365, 395
SspI AATATT 1 cut(s) 676
SspMI CTAG 2 cut(s) 125, 866
StyD4I CCNGG 3 cut(s) 53, 249, 383
StyI CCWWGG 1 cut(s) 94
TaaI ACNGT 5 cut(s) 139, 275, 379, 638, 892
TaiI ACGT 2 cut(s) 188, 745
TaqI TCGA 2 cut(s) 497, 539
TaqII GACCGA 1 cut(s) 107
TasI AATT 6 cut(s) 82, 484, 626, 720, 909, 920
TfiI GAWTC 3 cut(s) 217, 330, 536
Tru1I TTAA 8 cut(s) 176, 237, 405, 612, 629, 642, 918, 933
Tru9I TTAA 8 cut(s) 176, 237, 405, 612, 629, 642, 918, 933
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 1 cut(s) 831
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 3 cut(s) 302, 421, 951
TspGWI ACGGA 1 cut(s) 320
Van91I CCANNNNNTGG 1 cut(s) 631
VpaK11BI GGWCC 2 cut(s) 119, 307
VspI ATTAAT 1 cut(s) 629
XagI CCTNNNNNAGG 1 cut(s) 107
XspI CTAG 2 cut(s) 125, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.