AT3G25727

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012717
Physical Location & Seq
Reverse (-)
9393511 .. 9394334
824 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25727.1

Sequence Viewer

Length: 462 bp
ATGAGAAATGTCATGTTTCCTTATGAAATTGGGAACAGGAACCCAATTATTCAAATGCTCAATAACTGTCTTCCGGTTTCTAATGTGGTGAACCTCCAAGATGATGCAGAAGATGATCTCTTCTTGTGGAAGTTTGCAAGAGAAGTTTGGAATTATTTCACCTCTCGGGCCCATGTTTCTCCTCCTCATTTGTTTGGGGATGGAGTTAGATGGCTTAAGAATCCTTGTCATGACAAGAATGTGACTATGATTCTTAGGATTGCTCATCAAGCTTCGGTGTACACTCTATGGAAAGAACGAAACTCTCGACTTAACAATGCTACCTCAAGACCTGCTGCTGGAATTATTTTGGAGATAAAAGCTTTAATCCGCTGTCACTTGGATCCTTTGTCTAGAGCTCAGAGATTAGGACATCAAGGGACTTCCTTCCTCGCTACTTGGTTCGGCATGTTCAATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 zf-RVT

Protein Analysis

153

Amino Acids

17.66

Weight (kDa)

9.45

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 340
AciI CCGC 1 cut(s) 370
AclWI GGATC 2 cut(s) 377, 390
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 338
AflII CTTAAG 1 cut(s) 215
AgsI TTSAA 2 cut(s) 53, 454
AluBI AGCT 3 cut(s) 272, 362, 398
AluI AGCT 3 cut(s) 272, 362, 398
Alw21I GWGCWC 1 cut(s) 400
AlwI GGATC 2 cut(s) 377, 390
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 1 cut(s) 168
ApaI GGGCCC 1 cut(s) 172
ApeKI GCWGC 1 cut(s) 335
Asp700I GAANNNNTTC 1 cut(s) 155
AspS9I GGNCC 2 cut(s) 168, 169
AsuHPI GGTGA 2 cut(s) 100, 151
AvaI CYCGRG 1 cut(s) 165
BaeGI GKGCMC 1 cut(s) 172
BamHI GGATCC 1 cut(s) 382
BanII GRGCYC 2 cut(s) 172, 400
BbsI GAAGAC 1 cut(s) 62
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 1 cut(s) 322
BccI CCATC 2 cut(s) 194, 204
BfaI CTAG 1 cut(s) 393
BfrI CTTAAG 1 cut(s) 215
BfuAI ACCTGC 1 cut(s) 340
BisI GCNGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 337
BmeT110I CYCGRG 1 cut(s) 165
BmgT120I GGNCC 2 cut(s) 168, 169
BmiI GGNNCC 3 cut(s) 41, 170, 384
BmsI GCATC 1 cut(s) 94
BpiI GAAGAC 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 310
BsaWI WCCGGW 1 cut(s) 73
Bsc4I CCNNNNNNNGG 1 cut(s) 338
BseGI GGATG 1 cut(s) 205
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 1 cut(s) 413
BseRI GAGGAG 2 cut(s) 171, 174
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 1 cut(s) 322
BshFI GGCC 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 400
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 433
BslI CCNNNNNNNGG 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 433
BsnI GGCC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 165
Bsp120I GGGCCC 1 cut(s) 168
Bsp1286I GDGCHC 2 cut(s) 172, 400
Bsp1407I TGTACA 1 cut(s) 279
Bsp143I GATC 2 cut(s) 115, 382
BspACI CCGC 1 cut(s) 370
BspANI GGCC 1 cut(s) 170
BspCNI CTCAG 1 cut(s) 412
BspHI TCATGA 1 cut(s) 229
BspLI GGNNCC 3 cut(s) 41, 170, 384
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 2 cut(s) 377, 390
BspTI CTTAAG 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 279
BssMI GATC 2 cut(s) 115, 382
Bst4CI ACNGT 1 cut(s) 68
Bst6I CTCTTC 1 cut(s) 125
BstAFI CTTAAG 1 cut(s) 215
BstAUI TGTACA 1 cut(s) 279
BstDEI CTNAG 2 cut(s) 254, 399
BstF5I GGATG 1 cut(s) 205
BstKTI GATC 2 cut(s) 118, 385
BstMBI GATC 2 cut(s) 115, 382
BstMWI GCNNNNNNNGC 1 cut(s) 269
BstNSI RCATGY 1 cut(s) 451
BstSLI GKGCMC 1 cut(s) 172
BstV1I GCAGC 1 cut(s) 322
BstV2I GAAGAC 1 cut(s) 62
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BsuRI GGCC 1 cut(s) 170
BtsCI GGATG 1 cut(s) 205
BveI ACCTGC 1 cut(s) 340
CciI TCATGA 1 cut(s) 229
Cfr13I GGNCC 2 cut(s) 168, 169
Csp6I GTAC 1 cut(s) 280
CviAII CATG 4 cut(s) 13, 173, 230, 448
CviJI RGCY 5 cut(s) 170, 214, 272, 362, 398
CviKI_1 RGCY 5 cut(s) 170, 214, 272, 362, 398
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 2 cut(s) 254, 399
DpnI GATC 2 cut(s) 117, 384
DpnII GATC 2 cut(s) 115, 382
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Ecl136II GAGCTC 1 cut(s) 398
Eco24I GRGCYC 2 cut(s) 172, 400
Eco53kI GAGCTC 1 cut(s) 398
Eco88I CYCGRG 1 cut(s) 165
EcoICRI GAGCTC 1 cut(s) 398
EcoT38I GRGCYC 2 cut(s) 172, 400
FaeI CATG 4 cut(s) 16, 176, 233, 451
FaiI YATR 7 cut(s) 14, 24, 174, 231, 248, 289, 449
FaqI GGGAC 1 cut(s) 433
FatI CATG 4 cut(s) 12, 172, 229, 447
Fnu4HI GCNGC 1 cut(s) 336
FokI GGATG 1 cut(s) 212
FriOI GRGCYC 2 cut(s) 172, 400
Fsp4HI GCNGC 1 cut(s) 336
FspBI CTAG 1 cut(s) 393
GluI GCNGC 1 cut(s) 336
HaeIII GGCC 1 cut(s) 170
HapII CCGG 1 cut(s) 74
Hin1II CATG 4 cut(s) 16, 176, 233, 451
HindIII AAGCTT 2 cut(s) 270, 360
HinfI GANTC 2 cut(s) 220, 250
HpaII CCGG 1 cut(s) 74
HphI GGTGA 2 cut(s) 100, 151
Hpy166II GTNNAC 3 cut(s) 91, 280, 282
Hpy188I TCNGA 1 cut(s) 402
Hpy188III TCNNGA 4 cut(s) 230, 306, 327, 393
Hpy8I GTNNAC 3 cut(s) 91, 280, 282
HpyAV CCTTC 1 cut(s) 436
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4V TGCA 2 cut(s) 107, 137
HpyF10VI GCNNNNNNNGC 1 cut(s) 269
HpyF3I CTNAG 2 cut(s) 254, 399
Hsp92II CATG 4 cut(s) 16, 176, 233, 451
Kzo9I GATC 2 cut(s) 115, 382
LpnPI CCDG 4 cut(s) 22, 87, 324, 345
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 1 cut(s) 94
MaeI CTAG 1 cut(s) 393
MaeIII GTNAC 2 cut(s) 241, 374
MalI GATC 2 cut(s) 117, 384
MboI GATC 2 cut(s) 115, 382
MboII GAAGA 3 cut(s) 62, 112, 122
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 2 cut(s) 172, 400
MluCI AATT 4 cut(s) 27, 45, 151, 342
MnlI CCTC 6 cut(s) 104, 172, 192, 195, 334, 440
MroXI GAANNNNTTC 1 cut(s) 155
MseI TTAA 3 cut(s) 216, 312, 365
MspA1I CMGCKG 1 cut(s) 372
MspCI CTTAAG 1 cut(s) 215
MspI CCGG 1 cut(s) 74
MwoI GCNNNNNNNGC 1 cut(s) 269
NdeII GATC 2 cut(s) 115, 382
NlaIII CATG 4 cut(s) 16, 176, 233, 451
NlaIV GGNNCC 3 cut(s) 41, 170, 384
NmuCI GTSAC 2 cut(s) 241, 374
NspI RCATGY 1 cut(s) 451
PagI TCATGA 1 cut(s) 229
PcsI WCGNNNNNNNCGW 1 cut(s) 304
PdmI GAANNNNTTC 1 cut(s) 155
PfeI GAWTC 2 cut(s) 220, 250
PkrI GCNGC 1 cut(s) 337
Psp124BI GAGCTC 1 cut(s) 400
PspN4I GGNNCC 3 cut(s) 41, 170, 384
PspOMI GGGCCC 1 cut(s) 168
PspPI GGNCC 2 cut(s) 168, 169
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SacI GAGCTC 1 cut(s) 400
SaqAI TTAA 3 cut(s) 216, 312, 365
SatI GCNGC 1 cut(s) 336
Sau3AI GATC 2 cut(s) 115, 382
Sau96I GGNCC 2 cut(s) 168, 169
SduI GDGCHC 2 cut(s) 172, 400
SetI ASST 7 cut(s) 96, 164, 274, 326, 334, 364, 400
SfaNI GCATC 1 cut(s) 94
SmlI CTYRAG 2 cut(s) 215, 325
SmoI CTYRAG 2 cut(s) 215, 325
Sse9I AATT 4 cut(s) 27, 45, 151, 342
SsiI CCGC 1 cut(s) 370
SspMI CTAG 1 cut(s) 393
SstI GAGCTC 1 cut(s) 400
TaaI ACNGT 1 cut(s) 68
TaqI TCGA 1 cut(s) 307
TasI AATT 4 cut(s) 27, 45, 151, 342
TatI WGTACW 1 cut(s) 279
TfiI GAWTC 2 cut(s) 220, 250
Tru1I TTAA 3 cut(s) 216, 312, 365
Tru9I TTAA 3 cut(s) 216, 312, 365
TseFI GTSAC 2 cut(s) 241, 374
TseI GCWGC 1 cut(s) 335
Tsp45I GTSAC 2 cut(s) 241, 374
TspDTI ATGAA 1 cut(s) 39
Vha464I CTTAAG 1 cut(s) 215
XbaI TCTAGA 1 cut(s) 392
XceI RCATGY 1 cut(s) 451
XmnI GAANNNNTTC 1 cut(s) 155
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.