AT5G16486

zinc-binding in reverse transcriptase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012717
Physical Location & Seq
Reverse (-)
5383597 .. 5384226
630 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16486.1

Sequence Viewer

Length: 630 bp
ATGGTGAATATTAACTCGGGGAGCTGGATTTGGAAGAGTATTTGCAAGCTTCGTCCAATGGCTCGGGAGTTTGTGGTTTGCAAAGTCGGTTCGGGGATCACTTGTAATTTTTGGAGTGAGAATTGGACTAATTTGGGACCGCTTATTCATTTAACAGGTGACTTGGGACCTAGAGTTTCTGGTTTACCTAGGAATGCTTCAGTTGCAGATGCTTTAAGAGATGGGGTTTGGTGGATAAATGGGTCTAGGAGCAGGAACCCAATTATTCAGCTGTTAAAGAACTGTCTCCCTCTTTCTAGTGTGGTGAACCTTCAAGTGGATGCTGAAGATGATCTGTTCATGTGGAAGGTTGGGGGAAGTGAGGCTTCTGTTGGTTTCTCCTCTGCTGCAACGTGGATCCACTTAAATCCTGTTGGGGAAAAGGTTGATTGGCATAAGGCAATTTGGTTTAAAGGCAGGATTCCCAAGCATGCTTTCATCTCTTGGGTAAATATCAGACACAGGCTGCCTACTCGTGACAAACTTCTAAGCTGGGGACTTCATGTTCCATCTCTCTGCCTCCTTTGCAATGCTTTTGATGAGACGAGACAGCATCTCTTCTTTGACTGTGTGTTTGCAGGAGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RVT

Protein Analysis

209

Amino Acids

23.55

Weight (kDa)

8.93

Isoelectric Point (pI)

27.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 126 - 208 6.9e-24 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 140
AclWI GGATC 3 cut(s) 104, 391, 404
AcuI CTGAAG 2 cut(s) 183, 345
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 1 cut(s) 314
AjuI GAANNNNNNNTTGG 2 cut(s) 458, 490
AloI GAACNNNNNNTCC 2 cut(s) 528, 560
AluBI AGCT 4 cut(s) 24, 49, 271, 531
AluI AGCT 4 cut(s) 24, 49, 271, 531
Alw26I GTCTC 3 cut(s) 290, 575, 580
AlwI GGATC 3 cut(s) 104, 391, 404
Ama87I CYCGRG 2 cut(s) 16, 63
ApeKI GCWGC 2 cut(s) 386, 505
ArsI GACNNNNNNTTYG 2 cut(s) 596, 628
AspA2I CCTAGG 1 cut(s) 188
AspS9I GGNCC 2 cut(s) 137, 167
AsuHPI GGTGA 3 cut(s) 16, 170, 316
AvaI CYCGRG 2 cut(s) 16, 63
AvaII GGWCC 2 cut(s) 137, 167
AvrII CCTAGG 1 cut(s) 188
BamHI GGATCC 1 cut(s) 396
BauI CACGAG 1 cut(s) 513
BbvI GCAGC 2 cut(s) 373, 492
BccI CCATC 2 cut(s) 215, 556
BcoDI GTCTC 3 cut(s) 290, 575, 580
BfaI CTAG 4 cut(s) 171, 189, 246, 297
BisI GCNGC 2 cut(s) 387, 506
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 2 cut(s) 388, 507
Bme18I GGWCC 2 cut(s) 137, 167
BmeT110I CYCGRG 2 cut(s) 16, 63
BmgT120I GGNCC 2 cut(s) 137, 167
BmiI GGNNCC 4 cut(s) 138, 168, 257, 398
BmsI GCATC 3 cut(s) 199, 310, 601
BsaJI CCNNGG 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse3DI GCAATG 1 cut(s) 574
BseDI CCNNGG 1 cut(s) 188
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMI GCAATG 1 cut(s) 574
BseRI GAGGAG 1 cut(s) 370
BseXI GCAGC 2 cut(s) 373, 492
BseYI CCCAGC 1 cut(s) 531
BsiHKCI CYCGRG 2 cut(s) 16, 63
BslFI GGGAC 3 cut(s) 150, 180, 549
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 3 cut(s) 290, 575, 580
BsmBI CGTCTC 1 cut(s) 575
BsmFI GGGAC 3 cut(s) 150, 180, 549
BsmI GAATGC 1 cut(s) 199
BsoBI CYCGRG 2 cut(s) 16, 63
Bsp143I GATC 3 cut(s) 96, 331, 396
BspACI CCGC 1 cut(s) 140
BspLI GGNNCC 4 cut(s) 138, 168, 257, 398
BspPI GGATC 3 cut(s) 104, 391, 404
BsrDI GCAATG 1 cut(s) 574
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 3 cut(s) 96, 331, 396
BssSI CACGAG 1 cut(s) 513
BssT1I CCWWGG 1 cut(s) 188
Bst2BI CACGAG 1 cut(s) 513
Bst4CI ACNGT 2 cut(s) 284, 608
Bst6I CTCTTC 2 cut(s) 29, 602
BstC8I GCNNGC 2 cut(s) 47, 471
BstDEI CTNAG 1 cut(s) 527
BstF5I GGATG 1 cut(s) 325
BstKTI GATC 3 cut(s) 99, 334, 399
BstMAI GTCTC 3 cut(s) 290, 575, 580
BstMBI GATC 3 cut(s) 96, 331, 396
BstMWI GCNNNNNNNGC 2 cut(s) 203, 564
BstNSI RCATGY 1 cut(s) 473
BstV1I GCAGC 2 cut(s) 373, 492
BstX2I RGATCY 1 cut(s) 396
BstYI RGATCY 1 cut(s) 396
BtsCI GGATG 1 cut(s) 325
Cac8I GCNNGC 2 cut(s) 47, 471
Cfr13I GGNCC 2 cut(s) 137, 167
CviAII CATG 3 cut(s) 340, 470, 542
CviJI RGCY 7 cut(s) 24, 49, 62, 271, 365, 505, 531
CviKI_1 RGCY 7 cut(s) 24, 49, 62, 271, 365, 505, 531
DdeI CTNAG 1 cut(s) 527
DpnI GATC 3 cut(s) 98, 333, 398
DpnII GATC 3 cut(s) 96, 331, 396
DraI TTTAAA 1 cut(s) 451
Eam1104I CTCTTC 2 cut(s) 29, 602
EarI CTCTTC 2 cut(s) 29, 602
Eco130I CCWWGG 1 cut(s) 188
Eco47I GGWCC 2 cut(s) 137, 167
Eco57I CTGAAG 2 cut(s) 183, 345
Eco88I CYCGRG 2 cut(s) 16, 63
EcoO109I RGGNCCY 1 cut(s) 167
EcoT14I CCWWGG 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 188
Esp3I CGTCTC 1 cut(s) 575
FaeI CATG 3 cut(s) 343, 473, 545
FaiI YATR 4 cut(s) 341, 435, 471, 543
FalI AAGNNNNNCTT 2 cut(s) 349, 381
FaqI GGGAC 3 cut(s) 150, 180, 549
FatI CATG 3 cut(s) 339, 469, 541
Fnu4HI GCNGC 2 cut(s) 387, 506
FokI GGATG 1 cut(s) 332
Fsp4HI GCNGC 2 cut(s) 387, 506
FspBI CTAG 4 cut(s) 171, 189, 246, 297
GluI GCNGC 2 cut(s) 387, 506
GsaI CCCAGC 1 cut(s) 535
Hin1II CATG 3 cut(s) 343, 473, 545
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 1 cut(s) 460
HphI GGTGA 3 cut(s) 16, 170, 316
Hpy166II GTNNAC 2 cut(s) 185, 307
Hpy188I TCNGA 2 cut(s) 497, 629
Hpy188III TCNNGA 2 cut(s) 65, 515
Hpy8I GTNNAC 2 cut(s) 185, 307
HpyAV CCTTC 2 cut(s) 320, 340
HpyCH4III ACNGT 2 cut(s) 284, 608
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 6 cut(s) 45, 81, 206, 389, 567, 617
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 564
HpyF3I CTNAG 1 cut(s) 527
HpySE526I ACGT 1 cut(s) 392
Hsp92II CATG 3 cut(s) 343, 473, 545
Kzo9I GATC 3 cut(s) 96, 331, 396
LmnI GCTCC 2 cut(s) 21, 249
LpnPI CCDG 9 cut(s) 10, 141, 165, 238, 423, 442, 487, 517, 603
Lsp1109I GCAGC 2 cut(s) 373, 492
LweI GCATC 3 cut(s) 199, 310, 601
MaeI CTAG 4 cut(s) 171, 189, 246, 297
MaeII ACGT 1 cut(s) 392
MaeIII GTNAC 2 cut(s) 158, 515
MalI GATC 3 cut(s) 98, 333, 398
MboI GATC 3 cut(s) 96, 331, 396
MboII GAAGA 3 cut(s) 46, 338, 589
MflI RGATCY 1 cut(s) 396
MluCI AATT 5 cut(s) 106, 121, 130, 261, 441
MnlI CCTC 4 cut(s) 300, 355, 391, 569
MseI TTAA 6 cut(s) 12, 152, 215, 275, 404, 450
MspA1I CMGCKG 1 cut(s) 271
Mva1269I GAATGC 1 cut(s) 199
MwoI GCNNNNNNNGC 2 cut(s) 203, 564
NdeII GATC 3 cut(s) 96, 331, 396
NlaIII CATG 3 cut(s) 343, 473, 545
NlaIV GGNNCC 4 cut(s) 138, 168, 257, 398
NmuCI GTSAC 2 cut(s) 158, 515
NspI RCATGY 1 cut(s) 473
PaeI GCATGC 1 cut(s) 473
PctI GAATGC 1 cut(s) 199
PfeI GAWTC 1 cut(s) 460
PkrI GCNGC 2 cut(s) 388, 507
PpuMI RGGWCCY 1 cut(s) 167
Psp5II RGGWCCY 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 531
PspN4I GGNNCC 4 cut(s) 138, 168, 257, 398
PspPI GGNCC 2 cut(s) 137, 167
PspPPI RGGWCCY 1 cut(s) 167
PsuI RGATCY 1 cut(s) 396
PvuII CAGCTG 1 cut(s) 271
SaqAI TTAA 6 cut(s) 12, 152, 215, 275, 404, 450
SatI GCNGC 2 cut(s) 387, 506
Sau3AI GATC 3 cut(s) 96, 331, 396
Sau96I GGNCC 2 cut(s) 137, 167
SfaNI GCATC 3 cut(s) 199, 310, 601
SinI GGWCC 2 cut(s) 137, 167
SphI GCATGC 1 cut(s) 473
Sse9I AATT 5 cut(s) 106, 121, 130, 261, 441
SsiI CCGC 1 cut(s) 140
SspI AATATT 1 cut(s) 10
SspMI CTAG 4 cut(s) 171, 189, 246, 297
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 2 cut(s) 284, 608
TaiI ACGT 1 cut(s) 395
TasI AATT 5 cut(s) 106, 121, 130, 261, 441
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 6 cut(s) 12, 152, 215, 275, 404, 450
Tru9I TTAA 6 cut(s) 12, 152, 215, 275, 404, 450
TseFI GTSAC 2 cut(s) 158, 515
TseI GCWGC 2 cut(s) 386, 505
Tsp45I GTSAC 2 cut(s) 158, 515
TspDTI ATGAA 4 cut(s) 137, 328, 466, 530
VpaK11BI GGWCC 2 cut(s) 137, 167
XceI RCATGY 1 cut(s) 473
XmaJI CCTAGG 1 cut(s) 188
XspI CTAG 4 cut(s) 171, 189, 246, 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.