AT4G10613

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012717
Physical Location & Seq
Forward (+)
6560363 .. 6561726
1364 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10613.1

Sequence Viewer

Length: 441 bp
ATGTGGATTTCAAATCTCGATAGGCTGCCTACTCGTGCGAGGTTGGTTTCTTGGGGTCTGCAGATCTCACCTCTCTGTTGTTTATGCTCTCAGTCTGTTGAGACCCGGGACCATCTTATTCTCACTTGTGGATTCAGTTCTTCAATCTGGAACATGGTGCAGGCTAGGCTTAGGTTGCAGCCTGTTCAGTTCAGAGTTTGGGAAAGCTTACTATCTTGGATCAAGTTAAGTACGGCCTCCTCTCCTTCCATCATTAGGAAGCTTGTTGCTCAAGCCACTGTTTGTGCTATTTGGAAGCAGAGAAACAACCTTTTGCATAACTTACAAGACATTCTACCGTCTATCATCTTCAAGAATATAGACCGTGAGATCATCAATTCCATCAACGCAAGGTGTCATCGGAGAAAGTTTAGATCGCTTATGAGATTGTGGATCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RVT

Protein Analysis

146

Amino Acids

17.11

Weight (kDa)

11.23

Isoelectric Point (pI)

61.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 1 - 50 3.9e-13 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 227, 440
AfaI GTAC 1 cut(s) 232
AfiI CCNNNNNNNGG 1 cut(s) 255
AgsI TTSAA 3 cut(s) 12, 144, 352
AluBI AGCT 2 cut(s) 207, 262
AluI AGCT 2 cut(s) 207, 262
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 2 cut(s) 227, 440
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 2 cut(s) 25, 178
AspS9I GGNCC 1 cut(s) 109
AsuC2I CCSGG 2 cut(s) 106, 107
AsuHPI GGTGA 1 cut(s) 60
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 1 cut(s) 109
BauI CACGAG 1 cut(s) 33
BbvI GCAGC 2 cut(s) 12, 190
BccI CCATC 3 cut(s) 120, 257, 389
BceAI ACGGC 1 cut(s) 249
BcnI CCSGG 2 cut(s) 106, 107
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 1 cut(s) 165
BfmI CTRYAG 1 cut(s) 59
BglII AGATCT 1 cut(s) 63
BisI GCNGC 2 cut(s) 26, 179
BlsI GCNGC 2 cut(s) 27, 180
Bme1390I CCNGG 2 cut(s) 106, 107
Bme18I GGWCC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 110
BmrFI CCNGG 2 cut(s) 106, 107
Bpu10I CCTNAGC 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 255
BpuMI CCSGG 2 cut(s) 106, 107
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 105
Bsc4I CCNNNNNNNGG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 105
BseLI CCNNNNNNNGG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 104
BseRI GAGGAG 1 cut(s) 229
BseXI GCAGC 2 cut(s) 12, 190
BsgI GTGCAG 1 cut(s) 179
BshFI GGCC 1 cut(s) 236
BsiHKCI CYCGRG 1 cut(s) 105
BsiSI CCGG 1 cut(s) 106
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 1 cut(s) 255
BsmAI GTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 1 cut(s) 236
Bso31I GGTCTC 1 cut(s) 95
BsoBI CYCGRG 1 cut(s) 105
Bsp143I GATC 5 cut(s) 63, 219, 369, 413, 432
BspANI GGCC 1 cut(s) 236
BspCNI CTCAG 1 cut(s) 103
BspLI GGNNCC 1 cut(s) 110
BspMAI CTGCAG 1 cut(s) 63
BspPI GGATC 2 cut(s) 227, 440
BspTNI GGTCTC 1 cut(s) 95
BssECI CCNNGG 1 cut(s) 105
BssMI GATC 5 cut(s) 63, 219, 369, 413, 432
BssSI CACGAG 1 cut(s) 33
Bst2BI CACGAG 1 cut(s) 33
Bst4CI ACNGT 3 cut(s) 280, 339, 365
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 2 cut(s) 90, 170
BstKTI GATC 5 cut(s) 66, 222, 372, 416, 435
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 5 cut(s) 63, 219, 369, 413, 432
BstMWI GCNNNNNNNGC 2 cut(s) 166, 175
BstSCI CCNGG 2 cut(s) 104, 105
BstSFI CTRYAG 1 cut(s) 59
BstV1I GCAGC 2 cut(s) 12, 190
BstX2I RGATCY 1 cut(s) 63
BstYI RGATCY 1 cut(s) 63
BsuRI GGCC 1 cut(s) 236
BtsIMutI CAGTG 1 cut(s) 276
Cac8I GCNNGC 1 cut(s) 162
Cfr13I GGNCC 1 cut(s) 109
Cfr9I CCCGGG 1 cut(s) 105
Csp6I GTAC 1 cut(s) 231
CviAII CATG 1 cut(s) 154
CviJI RGCY 8 cut(s) 25, 164, 169, 181, 207, 236, 262, 275
CviKI_1 RGCY 8 cut(s) 25, 164, 169, 181, 207, 236, 262, 275
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 2 cut(s) 90, 170
DpnI GATC 5 cut(s) 65, 221, 371, 415, 434
DpnII GATC 5 cut(s) 63, 219, 369, 413, 432
Eco31I GGTCTC 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 105
FaeI CATG 1 cut(s) 157
FaiI YATR 5 cut(s) 85, 155, 318, 359, 422
FaqI GGGAC 1 cut(s) 122
FatI CATG 1 cut(s) 153
Fnu4HI GCNGC 2 cut(s) 26, 179
Fsp4HI GCNGC 2 cut(s) 26, 179
FspBI CTAG 1 cut(s) 165
GluI GCNGC 2 cut(s) 26, 179
HaeIII GGCC 1 cut(s) 236
HapII CCGG 1 cut(s) 106
Hin1II CATG 1 cut(s) 157
HindIII AAGCTT 2 cut(s) 205, 260
HinfI GANTC 1 cut(s) 132
HpaII CCGG 1 cut(s) 106
HphI GGTGA 1 cut(s) 60
Hpy188I TCNGA 3 cut(s) 194, 402, 437
Hpy188III TCNNGA 3 cut(s) 17, 148, 352
HpyAV CCTTC 1 cut(s) 255
HpyCH4III ACNGT 3 cut(s) 280, 339, 365
HpyCH4V TGCA 4 cut(s) 61, 160, 178, 316
HpyF10VI GCNNNNNNNGC 2 cut(s) 166, 175
HpyF3I CTNAG 2 cut(s) 90, 170
Hsp92II CATG 1 cut(s) 157
Kzo9I GATC 5 cut(s) 63, 219, 369, 413, 432
LpnPI CCDG 4 cut(s) 119, 133, 146, 195
Lsp1109I GCAGC 2 cut(s) 12, 190
MaeI CTAG 1 cut(s) 165
MalI GATC 5 cut(s) 65, 221, 371, 415, 434
MboI GATC 5 cut(s) 63, 219, 369, 413, 432
MboII GAAGA 2 cut(s) 132, 340
MflI RGATCY 1 cut(s) 63
MluCI AATT 1 cut(s) 376
MnlI CCTC 4 cut(s) 33, 81, 247, 250
MseI TTAA 1 cut(s) 227
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 2 cut(s) 106, 107
MwoI GCNNNNNNNGC 2 cut(s) 166, 175
NciI CCSGG 2 cut(s) 106, 107
NdeII GATC 5 cut(s) 63, 219, 369, 413, 432
NlaIII CATG 1 cut(s) 157
NlaIV GGNNCC 1 cut(s) 110
PfeI GAWTC 1 cut(s) 132
PkrI GCNGC 2 cut(s) 27, 180
PspN4I GGNNCC 1 cut(s) 110
PspPI GGNCC 1 cut(s) 109
PstI CTGCAG 1 cut(s) 63
PsuI RGATCY 1 cut(s) 63
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 227
SatI GCNGC 2 cut(s) 26, 179
Sau3AI GATC 5 cut(s) 63, 219, 369, 413, 432
Sau96I GGNCC 1 cut(s) 109
ScrFI CCNGG 2 cut(s) 106, 107
SetI ASST 7 cut(s) 44, 73, 176, 209, 264, 312, 395
SfcI CTRYAG 1 cut(s) 59
SinI GGWCC 1 cut(s) 109
SmaI CCCGGG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 270
SmoI CTYRAG 1 cut(s) 270
Sse9I AATT 1 cut(s) 376
SspMI CTAG 1 cut(s) 165
StyD4I CCNGG 2 cut(s) 104, 105
TaaI ACNGT 3 cut(s) 280, 339, 365
TaqI TCGA 1 cut(s) 18
TasI AATT 1 cut(s) 376
TfiI GAWTC 1 cut(s) 132
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TscAI CASTG 1 cut(s) 283
TseI GCWGC 2 cut(s) 25, 178
TspMI CCCGGG 1 cut(s) 105
TspRI CASTG 1 cut(s) 283
VpaK11BI GGWCC 1 cut(s) 109
XmaI CCCGGG 1 cut(s) 105
XspI CTAG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.