Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004154

Physical Location & Seq

Genomic Coordinates Reverse (-)

18569686 .. 18579182

Gene Structure 9497 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G50140.5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3012 bp

ATGTATACAAGGACAATAAGAAGGAACCAGAGATGGGGTTTTGTATTGCAGCAAGACAAATGCTTAGTTAGGCCAGTGATTCGTGACCACACCGGTTCCCAATCTTATAGATCCACCACAAGTATACTAACAGATCATTTGAGAAGAAACCATCATTCTCATGGTGCTATTGCCTCTAGAGATTATTTAAGTTATTCTTGGAATAGCCAATTGCGTCGGTTTAGTTCTGAAGGTGATGGAAATAATGCTAGTGAGGACAGCCGTTTCCCTCTGAATAAAGACAAGATGGGAAAAGAGAAGAGCACGGTCGGTGTTGAGCATTTTGATTCTCACGCTCAACTTGGAGTACAAGACCAAATAGAATGGCTCAACAATGAGAAGCGTGCAAGTGAAAGCAGAAAGGAGTCACCTTTTCTTAATAAACGAGAAAGATTGAAGAATGAGTTCTTGCGGAGAATTCAACCTTGGGAAACTATACAGCTCTCTTGGGAGTCTTTCCCTTACTACGTCCACGAACACACAAAAGATACTCTGGTGGAATGTGTTAGCTCACATATTAAGCAAAAAAGTATGACTTCCAAATATGGTGCTCGTTTAGATTCTTCAAGTGGGAGAATATTGCTCCAGAGTGTTCCAGGCACTGAGCTCTACCGAGAGAGGTTGGTGCGAGCACTTGCTCGAGATACACAAGTTCCCCTGTTGGTTCTTGACAGCAGCGTTCTTGCACCTTACGATTTTGCTGATGATTACAATGAAGAGTCTGAATCAGATGACGACATTGCAGAATCAGACCAGTGCACGTCACAGTCAGAAGCTGAAGAAGAAACCGATGGGGACAATGATGATACTAGTAGTAGTGAAACAAAAATAGAAGGAACTGACGACGAAGAAAGATATTTGGAGATTTCTAAGGAAGTCCTCAAGAAACTTGGGGCCGACATAGAGGATATCGAGAAGAGAATGTCAGAACAACTATATGGCTCTAGTGAGGTGTCAGAAGCTGCAGCTGTTGATCAAGATGATAAAGCGAAGCGGCCTCTTAAGAAAGGAGACCAAGTAAAGTATGTTGGGTCTCCTAAGAAGGACGAAGCAAAACATAGGGTCGTATTGGGGAAGATTTCCACATCTGATGGTCAAAAGAGTGCTTTTACCGTTATTCCTGGCAGGCCATTGTCTAGTGGACAACGTGGAGAGGTATATGAGGTGATTGGGAACCGTGTTGCTGTCATATTCGAATACGGTGATGATAAAACATCAGAGGGAAGTGAGAAAAAGCCAGCAGAGCAGCCCCAGATGTTACCCATCCACTGGTTAGATGTGAAAGACCTGAAGTACGATTTGGATATGCAGGCAGTTGATGGCTACATTGCTATGGAAGCTTTAAATGAGGTTTTGCAATCCATTCAACCACTTATAGTCTATTTTCCAGACTCGACGCAGTGGTTGTCTAGAGCTGTGCCTAAGACAAGACGAAAGGAATTTGTAGACAAAGTTAAGGAAATGTTTGATAAACTATCAGGTCCGATTGTTATGATCTGTGGGCAGAACAAGATAGAAACAGGCTCAAAAGAAAGAGAGAAATTCACGATGGTACTCCCAAACCTTAGCCGAGTTGTGAAGCTGCCTCTTCCCTTGAAGGGCCTAACTGAGGGATTCACGGGAAGGGGAAAATCAGAGGAGAATGAGATATACAAGCTTTTCACTAATGTTATGCGCCTACATCCTCCGAAGGAGGAGGATACCCTCAGATTATTCAAGAAACAACTAGGAGAAGATAGGAGAATTGTGATATCGCGGAGCAATATAAATGAGTTGCTAAAGGCACTTGAAGAACATGAGTTATTGTGCACAGATTTATATCAAGTGAACACCGATGGGGTTATATTGACAAAGCAAAAAGCAGAGAAAGCTATTGGCTGGGCCAAAAATCACTATTTAGCTTCTTGTCCAGTCCCATTGGTTAAAGGAGGTCGCTTGTCTCTGCCTCGTGAAAGCCTTGAGATATCAATTGCGAGGTTAAGAAAATTAGAAGATAACTCCCTAAAGCCTTCGCAAAACTTGAAGAATATTGCAAAGGATGAGTACGAAAGAAATTTTGTCTCGGCTGTGGTTGCTCCTGGAGAAATTGGCGTCAAATTTGAAGATATTGGGGCTCTCGAAGACGTGAAAAAAGCATTAAATGAGCTGGTTATTCTTCCCATGAGAAGGCCAGAGCTTTTTGCCCGTGGAAATCTCTTGCGGCCCTGTAAAGGAATATTGCTTTTTGGTCCTCCTGGAACGGGTAAGACGCTCCTAGCTAAGGCGCTTGCAACAGAAGCTGGGGCAAACTTCATTAGCATAACTGGCTCAACACTTACATCAAAGTGGTTCGGAGATGCAGAGAAGCTCACTAAGGCTCTCTTTTCCTTTGCCACCAAGCTAGCACCTGTAATTATTTTTGTTGATGAGATCGACAGTCTGCTAGGTGCTCGTGGTGGCTCTTCCGAGCACGAAGCTACCAGAAGAATGAGAAATGAGTTCATGGCAGCTTGGGACGGGCTCAGGTCAAAAGACAGCCAAAGGATCCTCATTCTTGGTGCCACCAACCGTCCCTTTGATCTTGATGATGCTGTCATTCGTCGTCTACCAAGGCGGATATATGTCGACTTACCAGATGCCGAAAACCGGTTAAAGATACTGAAAATATTTCTAACTCCTGAAAATCTGGAATCTGATTTCCAGTTCGAGAAACTTGCAAAGGAAACTGAAGGTTATTCCGGTAGCGATCTGAAGAATCTATGCATTGCTGCTGCGTACAGGCCTGTTCAAGAACTGTTACAAGAAGAACAAAAGGGTGCAAGAGCAGAAGCATCTCCTGGTCTACGGTCGCTAAGTTTGGATGACTTCATTCAGTCTAAAGCTAAGGTGAGCCCATCTGTGGCGTACGATGCGACTACGATGAATGAGCTGAGAAAATGGAACGAGCAATATGGTGAAGGAGGCAGCAGGACAAAGTCTCCTTTTGGCTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA

Protein Analysis

1003

Amino Acids

113.28

Weight (kDa)

6.12

Isoelectric Point (pI)

51.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	730 - 860	1.8e-37	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	883 - 919	1.7e-11	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004154)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50140 AT1G50140 AT1G50140 AT1G50140 AT1G50140 AT3G19740
fragaria_vesca	FvH4_4g36860 FvH4_4g36860 FvH4_4g36860
malus_domestica	MD13G1006500.v1.1 MD16G1001500.v1.1
prunus_persica	Prupe.1G346700_v2.0.a1 Prupe.1G346700_v2.0.a1 Prupe.1G346700_v2.0.a1 Prupe.1G346700_v2.0.a1 Prupe.1G346700_v2.0.a1 Prupe.1G346700_v2.0.a1
pyrus_communis	pycom13g00540 pycom16g00410
rosa_chinensis	RchiOBHm_Chr4g0446611
rosa_laevigata	RLG00000005645
rosa_multiflora	Rmu_sc0036387.1_g000001 Rmu_ssc0000144.1_g000003
rosa_roxburghii	Rroxscaffold_5G00387010
rosa_rugosa	Rorug04G0372800 Rorug04G0372900 Rorug04G0373000 Rorug04G0373100
rosa_samantha	Rh4AG434400 Rh4BG443600 Rh4CG460900 Rh4DG442400
rosa_wichuraiana	Rw4G037140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2576
AccB7I	CCANNNNNTGG	1 cut(s)	1308
AccI	GTMKAC	6 cut(s)	5, 124, 1485, 2623, 2643, 2860
AccII	CGCG	1 cut(s)	1795
AciI	CCGC	5 cut(s)	451, 1033, 1795, 2239, 2632
AclWI	GGATC	3 cut(s)	105, 2557, 2570
AcsI	RAATTY	5 cut(s)	456, 1478, 1580, 2092, 2135
AcuI	CTGAAG	5 cut(s)	249, 837, 1349, 2766, 2789
AcyI	GRCGYC	1 cut(s)	2130
AfaI	GTAC	6 cut(s)	348, 1334, 1593, 2084, 2795, 2924
AflII	CTTAAG	1 cut(s)	1040
AgeI	ACCGGT	2 cut(s)	92, 2664
AhlI	ACTAGT	1 cut(s)	848
AjiI	CACGTC	2 cut(s)	801, 2164
AjnI	CCWGG	5 cut(s)	634, 1159, 2116, 2272, 2854
AjuI	GAANNNNNNNTTGG	4 cut(s)	448, 480, 1322, 1354
Alw21I	GWGCWC	8 cut(s)	305, 592, 648, 673, 800, 1850, 2470, 2490
Alw26I	GTCTC	5 cut(s)	1044, 1077, 1983, 2104, 3000
Alw44I	GTGCAC	2 cut(s)	796, 1846
AlwI	GGATC	3 cut(s)	105, 2557, 2570
AlwNI	CAGNNNCTG	4 cut(s)	641, 815, 1001, 2318
Ama87I	CYCGRG	1 cut(s)	678
AoxI	GGCC	9 cut(s)	71, 933, 1034, 1166, 1639, 1920, 2207, 2240, 2798
ApaLI	GTGCAC	2 cut(s)	796, 1846
ApoI	RAATTY	5 cut(s)	456, 1478, 1580, 2092, 2135
AsiGI	ACCGGT	2 cut(s)	92, 2664
Asp700I	GAANNNNTTC	1 cut(s)	443
AspLEI	GCGC	2 cut(s)	1716, 2305
AspS9I	GGNCC	6 cut(s)	933, 1520, 1639, 1920, 2241, 2267
AsuHPI	GGTGA	6 cut(s)	245, 399, 1216, 1253, 2917, 2984
AsuII	TTCGAA	1 cut(s)	1233
AsuNHI	GCTAGC	1 cut(s)	2419
AvaI	CYCGRG	1 cut(s)	678
AvaII	GGWCC	2 cut(s)	1520, 2267
BaeGI	GKGCMC	2 cut(s)	800, 1850
BaeI	ACNNNNGTAYC	4 cut(s)	519, 552, 675, 708
BamHI	GGATCC	1 cut(s)	2562
BanI	GGYRCC	1 cut(s)	2576
BanII	GRGCYC	4 cut(s)	648, 2155, 2541, 2912
BauI	CACGAG	2 cut(s)	1986, 2469
BbsI	GAAGAC	1 cut(s)	2166
Bbv12I	GWGCWC	8 cut(s)	305, 592, 648, 673, 800, 1850, 2470, 2490
BceAI	ACGGC	1 cut(s)	246
BcgI	CGANNNNNNTGC	2 cut(s)	2714, 2748
BciT130I	CCWGG	5 cut(s)	636, 1161, 2118, 2274, 2856
BciVI	GTATCC	1 cut(s)	1732
BclI	TGATCA	1 cut(s)	1012
BcoDI	GTCTC	5 cut(s)	1044, 1077, 1983, 2104, 3000
BcuI	ACTAGT	1 cut(s)	848
BfmI	CTRYAG	1 cut(s)	1002
BfoI	RGCGCY	1 cut(s)	2306
BfrI	CTTAAG	1 cut(s)	1040
BfuI	GTATCC	1 cut(s)	1732
Bme1390I	CCNGG	5 cut(s)	636, 1161, 2118, 2274, 2856
Bme18I	GGWCC	2 cut(s)	1520, 2267
BmeT110I	CYCGRG	1 cut(s)	678
BmgBI	CACGTC	2 cut(s)	801, 2164
BmgT120I	GGNCC	6 cut(s)	933, 1520, 1639, 1920, 2241, 2267
BmiI	GGNNCC	6 cut(s)	26, 97, 934, 1214, 2564, 2578
BmrFI	CCNGG	5 cut(s)	636, 1161, 2118, 2274, 2856
BmsI	GCATC	5 cut(s)	2365, 2596, 2644, 2858, 2917
BmtI	GCTAGC	1 cut(s)	2423
BpiI	GAAGAC	1 cut(s)	2166
BpmI	CTGGAG	2 cut(s)	608, 2139
Bpu10I	CCTNAGC	4 cut(s)	1604, 2298, 2540, 2901
Bpu14I	TTCGAA	1 cut(s)	1233
BpuEI	CTTGAG	2 cut(s)	905, 2018
BsaBI	GATNNNNATC	1 cut(s)	1857
BsaHI	GRCGYC	1 cut(s)	2130
BsaI	GGTCTC	2 cut(s)	1044, 1077
BsaJI	CCNNGG	3 cut(s)	464, 2224, 2627
BsaWI	WCCGGW	3 cut(s)	92, 2664, 2756
BsaXI	ACNNNNNCTCC	4 cut(s)	1041, 1071, 2980, 3010
Bse118I	RCCGGY	2 cut(s)	92, 2664
Bse1I	ACTGG	6 cut(s)	74, 793, 1313, 1949, 2347, 2719
Bse3DI	GCAATG	3 cut(s)	777, 1365, 2781
Bse8I	GATNNNNATC	1 cut(s)	1857
BseBI	CCWGG	5 cut(s)	636, 1161, 2118, 2274, 2856
BseDI	CCNNGG	3 cut(s)	464, 2224, 2627
BseGI	GGATG	4 cut(s)	1302, 1720, 2083, 2884
BseJI	GATNNNNATC	1 cut(s)	1857
BseMI	GCAATG	3 cut(s)	777, 1365, 2781
BseMII	CTCAG	5 cut(s)	633, 1638, 1759, 2554, 2939
BseNI	ACTGG	6 cut(s)	74, 793, 1313, 1949, 2347, 2719
BseRI	GAGGAG	2 cut(s)	1691, 1748
BseSI	GKGCMC	2 cut(s)	800, 1850
BseYI	CCCAGC	2 cut(s)	1917, 2318
Bsh1236I	CGCG	1 cut(s)	1795
Bsh1285I	CGRYCG	2 cut(s)	309, 2867
BshFI	GGCC	9 cut(s)	73, 935, 1036, 1168, 1641, 1922, 2209, 2242, 2800
BshNI	GGYRCC	1 cut(s)	2576
BshTI	ACCGGT	2 cut(s)	92, 2664
BsiEI	CGRYCG	2 cut(s)	309, 2867
BsiHKAI	GWGCWC	8 cut(s)	305, 592, 648, 673, 800, 1850, 2470, 2490
BsiHKCI	CYCGRG	1 cut(s)	678
BsiSI	CCGG	3 cut(s)	93, 2665, 2757
BsiWI	CGTACG	1 cut(s)	2922
BslFI	GGGAC	4 cut(s)	848, 1937, 2546, 2574
BsmAI	GTCTC	5 cut(s)	1044, 1077, 1983, 2104, 3000
BsmFI	GGGAC	4 cut(s)	848, 1937, 2546, 2574
BsnI	GGCC	9 cut(s)	73, 935, 1036, 1168, 1641, 1922, 2209, 2242, 2800
Bso31I	GGTCTC	2 cut(s)	1044, 1077
BsoBI	CYCGRG	1 cut(s)	678
Bsp119I	TTCGAA	1 cut(s)	1233
Bsp143I	GATC	8 cut(s)	110, 133, 1012, 1533, 2448, 2562, 2596, 2764
BspACI	CCGC	5 cut(s)	451, 1033, 1795, 2239, 2632
BspANI	GGCC	9 cut(s)	73, 935, 1036, 1168, 1641, 1922, 2209, 2242, 2800
BspCNI	CTCAG	5 cut(s)	634, 1639, 1758, 2553, 2940
BspFNI	CGCG	1 cut(s)	1795
BspLI	GGNNCC	6 cut(s)	26, 97, 934, 1214, 2564, 2578
BspMAI	CTGCAG	1 cut(s)	1006
BspOI	GCTAGC	1 cut(s)	2423
BspPI	GGATC	3 cut(s)	105, 2557, 2570
BspQI	GCTCTTC	2 cut(s)	293, 2485
BspT104I	TTCGAA	1 cut(s)	1233
BspT107I	GGYRCC	1 cut(s)	2576
BspTI	CTTAAG	1 cut(s)	1040
BspTNI	GGTCTC	2 cut(s)	1044, 1077
BsrDI	GCAATG	3 cut(s)	777, 1365, 2781
BsrFI	RCCGGY	2 cut(s)	92, 2664
BsrI	ACTGG	6 cut(s)	74, 793, 1313, 1949, 2347, 2719
BssAI	RCCGGY	2 cut(s)	92, 2664
BssECI	CCNNGG	3 cut(s)	464, 2224, 2627
BssMI	GATC	8 cut(s)	110, 133, 1012, 1533, 2448, 2562, 2596, 2764
BssNAI	GTATAC	2 cut(s)	6, 125
BssNI	GRCGYC	1 cut(s)	2130
BssSI	CACGAG	2 cut(s)	1986, 2469
BssT1I	CCWWGG	2 cut(s)	464, 2627
Bst1107I	GTATAC	2 cut(s)	6, 125
Bst2BI	CACGAG	2 cut(s)	1986, 2469
Bst2UI	CCWGG	5 cut(s)	636, 1161, 2118, 2274, 2856
Bst4CI	ACNGT	9 cut(s)	307, 807, 1153, 1217, 1241, 2456, 2588, 2814, 2865
Bst6I	CTCTTC	5 cut(s)	293, 750, 950, 1632, 2485
BstACI	GRCGYC	1 cut(s)	2130
BstAFI	CTTAAG	1 cut(s)	1040
BstBI	TTCGAA	1 cut(s)	1233
BstC8I	GCNNGC	7 cut(s)	384, 669, 1166, 1278, 1350, 2307, 2421
BstDSI	CCRYGG	1 cut(s)	2224
BstENI	CCTNNNNNAGG	2 cut(s)	1646, 2297
BstF5I	GGATG	4 cut(s)	1302, 1720, 2083, 2884
BstFNI	CGCG	1 cut(s)	1795
BstH2I	RGCGCY	1 cut(s)	2306
BstHHI	GCGC	2 cut(s)	1716, 2305
BstKTI	GATC	8 cut(s)	113, 136, 1015, 1536, 2451, 2565, 2599, 2767
BstMAI	GTCTC	5 cut(s)	1044, 1077, 1983, 2104, 3000
BstMBI	GATC	8 cut(s)	110, 133, 1012, 1533, 2448, 2562, 2596, 2764
BstMCI	CGRYCG	2 cut(s)	309, 2867
BstMWI	GCNNNNNNNGC	7 cut(s)	1282, 1376, 1907, 2111, 2315, 2343, 2927
BstNI	CCWGG	5 cut(s)	636, 1161, 2118, 2274, 2856
BstSCI	CCNGG	5 cut(s)	634, 1159, 2116, 2272, 2854
BstSFI	CTRYAG	1 cut(s)	1002
BstSLI	GKGCMC	2 cut(s)	800, 1850
BstUI	CGCG	1 cut(s)	1795
BstV2I	GAAGAC	1 cut(s)	2166
BstX2I	RGATCY	2 cut(s)	110, 2562
BstYI	RGATCY	2 cut(s)	110, 2562
BstZ17I	GTATAC	2 cut(s)	6, 125
BsuI	GTATCC	1 cut(s)	1732
BsuRI	GGCC	9 cut(s)	73, 935, 1036, 1168, 1641, 1922, 2209, 2242, 2800
BtgI	CCRYGG	1 cut(s)	2224
BtrI	CACGTC	2 cut(s)	801, 2164
BtsCI	GGATG	4 cut(s)	1302, 1720, 2083, 2884
BtsI	GCAGTG	1 cut(s)	1445
BtsIMutI	CAGTG	5 cut(s)	81, 639, 800, 1306, 1445
Cac8I	GCNNGC	7 cut(s)	384, 669, 1166, 1278, 1350, 2307, 2421
CaiI	CAGNNNCTG	4 cut(s)	641, 815, 1001, 2318
CfoI	GCGC	2 cut(s)	1716, 2305
Cfr10I	RCCGGY	2 cut(s)	92, 2664
Cfr13I	GGNCC	6 cut(s)	933, 1520, 1639, 1920, 2241, 2267
CseI	GACGC	4 cut(s)	203, 1444, 2119, 2296
Csp6I	GTAC	6 cut(s)	347, 1333, 1592, 2083, 2794, 2923
CspAI	ACCGGT	2 cut(s)	92, 2664
CviAII	CATG	4 cut(s)	161, 1835, 2200, 2521
CviQI	GTAC	6 cut(s)	347, 1333, 1592, 2083, 2794, 2923
DpnI	GATC	8 cut(s)	112, 135, 1014, 1535, 2450, 2564, 2598, 2766
DpnII	GATC	8 cut(s)	110, 133, 1012, 1533, 2448, 2562, 2596, 2764
DraI	TTTAAA	1 cut(s)	1383
Eam1104I	CTCTTC	5 cut(s)	293, 750, 950, 1632, 2485
EarI	CTCTTC	5 cut(s)	293, 750, 950, 1632, 2485
EciI	GGCGGA	1 cut(s)	2647
Ecl136II	GAGCTC	1 cut(s)	646
Eco130I	CCWWGG	2 cut(s)	464, 2627
Eco147I	AGGCCT	1 cut(s)	2800
Eco24I	GRGCYC	4 cut(s)	648, 2155, 2541, 2912
Eco31I	GGTCTC	2 cut(s)	1044, 1077
Eco32I	GATATC	3 cut(s)	949, 1791, 2004
Eco47I	GGWCC	2 cut(s)	1520, 2267
Eco53kI	GAGCTC	1 cut(s)	646
Eco57I	CTGAAG	5 cut(s)	249, 837, 1349, 2766, 2789
Eco88I	CYCGRG	1 cut(s)	678
EcoICRI	GAGCTC	1 cut(s)	646
EcoNI	CCTNNNNNAGG	2 cut(s)	1646, 2297
EcoO109I	RGGNCCY	1 cut(s)	1639
EcoRI	GAATTC	1 cut(s)	456
EcoRII	CCWGG	5 cut(s)	634, 1159, 2116, 2272, 2854
EcoRV	GATATC	3 cut(s)	949, 1791, 2004
EcoT14I	CCWWGG	2 cut(s)	464, 2627
EcoT22I	ATGCAT	1 cut(s)	2783
EcoT38I	GRGCYC	4 cut(s)	648, 2155, 2541, 2912
ErhI	CCWWGG	2 cut(s)	464, 2627
FaeI	CATG	4 cut(s)	164, 1838, 2203, 2524
FalI	AAGNNNNNCTT	6 cut(s)	181, 213, 559, 591, 2384, 2416
FaqI	GGGAC	4 cut(s)	848, 1937, 2546, 2574
FatI	CATG	4 cut(s)	160, 1834, 2199, 2520
FbaI	TGATCA	1 cut(s)	1012
FblI	GTMKAC	6 cut(s)	5, 124, 1485, 2623, 2643, 2860
FokI	GGATG	4 cut(s)	1289, 1707, 2090, 2891
FriOI	GRGCYC	4 cut(s)	648, 2155, 2541, 2912
GlaI	GCGC	2 cut(s)	1715, 2304
GsaI	CCCAGC	2 cut(s)	1921, 2322
GsuI	CTGGAG	2 cut(s)	608, 2139
HaeII	RGCGCY	1 cut(s)	2306
HaeIII	GGCC	9 cut(s)	73, 935, 1036, 1168, 1641, 1922, 2209, 2242, 2800
HapII	CCGG	3 cut(s)	93, 2665, 2757
HgaI	GACGC	4 cut(s)	203, 1444, 2119, 2296
HhaI	GCGC	2 cut(s)	1716, 2305
Hin1I	GRCGYC	1 cut(s)	2130
Hin1II	CATG	4 cut(s)	164, 1838, 2203, 2524
Hin6I	GCGC	2 cut(s)	1714, 2303
HinP1I	GCGC	2 cut(s)	1714, 2303
HincII	GTYRAC	1 cut(s)	2644
HindII	GTYRAC	1 cut(s)	2644
HindIII	AAGCTT	2 cut(s)	1377, 1694
HpaII	CCGG	3 cut(s)	93, 2665, 2757
HphI	GGTGA	6 cut(s)	245, 399, 1216, 1253, 2917, 2984
Hpy99I	CGWCG	4 cut(s)	219, 887, 1438, 2622
HpyCH4III	ACNGT	9 cut(s)	307, 807, 1153, 1217, 1241, 2456, 2588, 2814, 2865
HpyCH4IV	ACGT	4 cut(s)	507, 800, 1186, 2163
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1282, 1376, 1907, 2111, 2315, 2343, 2927
HpySE526I	ACGT	4 cut(s)	507, 800, 1186, 2163
Hsp92I	GRCGYC	1 cut(s)	2130
Hsp92II	CATG	4 cut(s)	164, 1838, 2203, 2524
HspAI	GCGC	2 cut(s)	1714, 2303
Ksp22I	TGATCA	1 cut(s)	1012
Kzo9I	GATC	8 cut(s)	110, 133, 1012, 1533, 2448, 2562, 2596, 2764
LguI	GCTCTTC	2 cut(s)	293, 2485
LmnI	GCTCC	4 cut(s)	627, 1797, 2119, 2295
LweI	GCATC	5 cut(s)	2365, 2596, 2644, 2858, 2917
MaeII	ACGT	4 cut(s)	507, 800, 1186, 2163
MaeIII	GTNAC	5 cut(s)	83, 405, 801, 1296, 2814
MalI	GATC	8 cut(s)	112, 135, 1014, 1535, 2450, 2564, 2598, 2766
MboI	GATC	8 cut(s)	110, 133, 1012, 1533, 2448, 2562, 2596, 2764
MfeI	CAATTG	2 cut(s)	209, 2007
MflI	RGATCY	2 cut(s)	110, 2562
MlyI	GAGTC	4 cut(s)	413, 500, 767, 1424
Mph1103I	ATGCAT	1 cut(s)	2783
MroXI	GAANNNNTTC	1 cut(s)	443
MslI	CAYNNNNRTG	3 cut(s)	159, 1370, 2362
MspA1I	CMGCKG	1 cut(s)	1007
MspCI	CTTAAG	1 cut(s)	1040
MspI	CCGG	3 cut(s)	93, 2665, 2757
MspR9I	CCNGG	5 cut(s)	636, 1161, 2118, 2274, 2856
MunI	CAATTG	2 cut(s)	209, 2007
MvaI	CCWGG	5 cut(s)	636, 1161, 2118, 2274, 2856
MvnI	CGCG	1 cut(s)	1795
MwoI	GCNNNNNNNGC	7 cut(s)	1282, 1376, 1907, 2111, 2315, 2343, 2927
NdeII	GATC	8 cut(s)	110, 133, 1012, 1533, 2448, 2562, 2596, 2764
NheI	GCTAGC	1 cut(s)	2419
NlaIII	CATG	4 cut(s)	164, 1838, 2203, 2524
NlaIV	GGNNCC	6 cut(s)	26, 97, 934, 1214, 2564, 2578
NmeAIII	GCCGAG	2 cut(s)	1634, 2081
NmuCI	GTSAC	3 cut(s)	83, 405, 801
NsiI	ATGCAT	1 cut(s)	2783
NspV	TTCGAA	1 cut(s)	1233
PaeR7I	CTCGAG	1 cut(s)	678
PceI	AGGCCT	1 cut(s)	2800
PciSI	GCTCTTC	2 cut(s)	293, 2485
PdmI	GAANNNNTTC	1 cut(s)	443
PfeI	GAWTC	8 cut(s)	79, 326, 599, 764, 785, 1653, 2708, 2773
Pfl23II	CGTACG	1 cut(s)	2922
PflFI	GACNNNGTC	1 cut(s)	2992
PflMI	CCANNNNNTGG	1 cut(s)	1308
PfoI	TCCNGGA	2 cut(s)	2116, 2272
PinAI	ACCGGT	2 cut(s)	92, 2664
PleI	GAGTC	4 cut(s)	412, 499, 766, 1424
PpsI	GAGTC	4 cut(s)	412, 499, 766, 1424
Psp124BI	GAGCTC	1 cut(s)	648
Psp6I	CCWGG	5 cut(s)	634, 1159, 2116, 2272, 2854
PspFI	CCCAGC	2 cut(s)	1917, 2318
PspGI	CCWGG	5 cut(s)	634, 1159, 2116, 2272, 2854
PspLI	CGTACG	1 cut(s)	2922
PspN4I	GGNNCC	6 cut(s)	26, 97, 934, 1214, 2564, 2578
PspPI	GGNCC	6 cut(s)	933, 1520, 1639, 1920, 2241, 2267
PstI	CTGCAG	1 cut(s)	1006
PstNI	CAGNNNCTG	4 cut(s)	641, 815, 1001, 2318
PsuI	RGATCY	2 cut(s)	110, 2562
PsyI	GACNNNGTC	1 cut(s)	2992
PvuII	CAGCTG	1 cut(s)	1007
RsaI	GTAC	6 cut(s)	348, 1334, 1593, 2084, 2795, 2924
RsaNI	GTAC	6 cut(s)	347, 1333, 1592, 2083, 2794, 2923
RseI	CAYNNNNRTG	3 cut(s)	159, 1370, 2362
SacI	GAGCTC	1 cut(s)	648
SalI	GTCGAC	1 cut(s)	2642
SapI	GCTCTTC	2 cut(s)	293, 2485
Sau3AI	GATC	8 cut(s)	110, 133, 1012, 1533, 2448, 2562, 2596, 2764
Sau96I	GGNCC	6 cut(s)	933, 1520, 1639, 1920, 2241, 2267
SchI	GAGTC	4 cut(s)	413, 500, 767, 1424
ScrFI	CCNGG	5 cut(s)	636, 1161, 2118, 2274, 2856
SfaNI	GCATC	5 cut(s)	2365, 2596, 2644, 2858, 2917
SfcI	CTRYAG	1 cut(s)	1002
Sfr274I	CTCGAG	1 cut(s)	678
SfuI	TTCGAA	1 cut(s)	1233
SinI	GGWCC	2 cut(s)	1520, 2267
SlaI	CTCGAG	1 cut(s)	678
SmiMI	CAYNNNNRTG	3 cut(s)	159, 1370, 2362
SmlI	CTYRAG	4 cut(s)	678, 920, 1040, 1997
SmoI	CTYRAG	4 cut(s)	678, 920, 1040, 1997
SpeI	ACTAGT	1 cut(s)	848
SseBI	AGGCCT	1 cut(s)	2800
SsiI	CCGC	5 cut(s)	451, 1033, 1795, 2239, 2632
SspI	AATATT	4 cut(s)	618, 2068, 2256, 2685
SstI	GAGCTC	1 cut(s)	648
StuI	AGGCCT	1 cut(s)	2800
StyD4I	CCNGG	5 cut(s)	634, 1159, 2116, 2272, 2854
StyI	CCWWGG	2 cut(s)	464, 2627
TaaI	ACNGT	9 cut(s)	307, 807, 1153, 1217, 1241, 2456, 2588, 2814, 2865
TaiI	ACGT	4 cut(s)	510, 803, 1189, 2166
TaqI	TCGA	8 cut(s)	679, 951, 1233, 1433, 2157, 2451, 2643, 2724
TatI	WGTACW	1 cut(s)	346
TauI	GCSGC	2 cut(s)	1036, 2242
TfiI	GAWTC	8 cut(s)	79, 326, 599, 764, 785, 1653, 2708, 2773
TscAI	CASTG	5 cut(s)	81, 646, 800, 1313, 1445
TseFI	GTSAC	3 cut(s)	83, 405, 801
Tsp45I	GTSAC	3 cut(s)	83, 405, 801
TspDTI	ATGAA	5 cut(s)	768, 2320, 2509, 2875, 2954
TspRI	CASTG	5 cut(s)	81, 646, 800, 1313, 1445
Tth111I	GACNNNGTC	1 cut(s)	2992
Van91I	CCANNNNNTGG	1 cut(s)	1308
Vha464I	CTTAAG	1 cut(s)	1040
VneI	GTGCAC	2 cut(s)	796, 1846
VpaK11BI	GGWCC	2 cut(s)	1520, 2267
XagI	CCTNNNNNAGG	2 cut(s)	1646, 2297
XapI	RAATTY	5 cut(s)	456, 1478, 1580, 2092, 2135
XbaI	TCTAGA	2 cut(s)	176, 1448
XcmI	CCANNNNNNNNNTGG	1 cut(s)	158
XhoI	CTCGAG	1 cut(s)	678
XmiI	GTMKAC	6 cut(s)	5, 124, 1485, 2623, 2643, 2860
XmnI	GAANNNNTTC	1 cut(s)	443
Zsp2I	ATGCAT	1 cut(s)	2783

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.