Rroxscaffold_5G00387010

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004154
Physical Location & Seq
Forward (+)
65748799 .. 65765242
16444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00387010.1

Sequence Viewer

Length: 4248 bp
ATGGAATGTGCTGCTGCTCATTTGAAACATAAAAAGTTCACTTCTACTTATGGTTCTCGTTTGACATCTTCAAGCGGGAGAATACTGCTTCAGAGTGCTCCAGGCACTGAGCTTTACCGTGAAAGATTAGTTAGAGCTCTTGCGCGAGATCTTCAAGTTCCACTACTTGTGCTTGACAGCAGTGTTCTTGCTCCTTATGATTTTGGTGATGATTGTCAAACGGAGTCTGAATCAGAGGATGATGGTGTGGAGGAGTGTACTTCAGAGTCAGAGATTGATGATGAGAATGATGCGAGTAATGAAGAAGAATGGACTAGTAGTAATGAGGCAAAGTCAGACTGTAGTGATAAAGATGAAGTTGATGTGCATGCAAAGGCTGAAGCAGCCCTCAAGAAGCTTGTTCCTCTTGAACAATTTTCTAAGATGGTATCTGGGGAAATTAATGTTGAATCTGAGTCCTCAAAGTCAGAAGCTGCTGAGCCTACCGATAAATCCAAAGAATCCAAACGACCACTGAAGAAAGGTGATCGGGTGAAGTATATTGGGCCTACTTTACGTGTTGAAGCTGATAACAGGATCATATTGGGGAAAATATCAACATCTGATGGTCCAAGGAAAGCATATACCATTTTTCGTGGCAGGCCTTTACCGAATGGTCAACGTGGGGAGGTATATGAGGTGAGCGGAGATCGAATTGCTGTTATATTGGATATAAATGATGACACAGGAAGTGATGTGGATAAAGAGGAGAAAGGAGAAGACCAGCCTGCAAACCCACCAGTTTATTGGATACATGCAAATCATGTCGAGCATGTAGATGAGACTCAGACTGAGGATTGTTATATTGCAATGGAGGCCCTCCGTGAGGTATTGCATGCCAAGCAACCTCTTATTGTCTATTTTCCAGACTCTTCTCAATGGTTGTCTAGGGCAGTTCCCAAGTCAAACAGGAAGGACTTTGTCAATAAGGTGCAAGAAATTTTTGACCAGTTATCAGGTCCTGTTGTTTTAATCTGTGGACAGAACAAAACTGAATCCGAGTCAAAGGAGAAAGAGAAATTTACGATGATACTACCAAATTTTGGTCGTCTTGCAAAGCTGCCTGTTTCTCTTAAGCGTCTTACTGAGGGACTTAAGGCAACAAAGCGATCTGATGAGGATGAAATATATAAGCTCTTCTCAAATGTGTTCTGCATACATCCCCCAAAGGAAGAAGAGGTACTTCGGACATTCAATAAACAGATTGAAGAAGATGGGAGAATTGTGATGTCAAGGAGCAATTTGAATGAATTACATAAGGTTCTCGAGGAACATGAGCTATCATGCATGGACCTATTGCAAGTAGATACTGATGGTGTGATCTTGACAAAGCGAAAAGCTGAGAAAGTAGTTGGCTGGGCTAAAAGCCATTACTTATCATCTTGTCTTGTTCCTTCCGTTAAAGGTGACAGACTGCAGCTTCCTCGTGAAAGCCTTGAAAACCTTGCGAAGGATGAATATGAGAGTAACTTTGTTTCAGCAGTTGTACCTCCTGGTGAAATTGGTGTCAGGTTTGAGGATGTAGGTGCTCTGGAGGAGGTGAAGAAGGCTTTGAATGAACTAGTCATTCTTCCAATGAGAAGACCAGAGCTTTTCTCTCATGGAAACTTGTTGCGGCCTTGCAAAGGGATATTACTTTTTGGTCCTCCTGGAACTGGTAAGACCCTTCTTGCCAAGGCACTTGCGACAGAAGCTGGGGCAAATTTCATCAGCATTACTGGTTCAACTCTAACATCAAAGTGGTTTGGAGATGCTGAAAAGCTGACCAAAGCTCTCTTCTCTTTTGCCAGCAAGCTTGCTCCCGTCATTATATTTGTCGATGAGGTTGATAGTTTGCTTGGTGCTCGTGGTGGTTCTTTTGAGCATGAGGCAACTAGAAGAATGAGAAATGAGTTCATGGCAGCTTGGGATGGATTGCGATCCAAAGACAGTCAAAGAATTCTTATTCTCGGAGCCACAAATCGGCCATTTGACCTTGATGATGCTGTTATTCGTCGCTTACCAAGAAGGATATATGTTGATTTACCAGATGTTGAAAATCGCAAGAAGATTTTGAGTATCTTTCTTGCTCAAGAGAATCTAGAACCTGCTTTCCAATTTGATAAACTTTCAGAGGCAACCGAGGGATACTCTGGCAGTGATTTGAAGAACCTCTGTATTGCTGCTGCATATAGGCCTGTCCAGGAACTATTAGAAGAAGAAACCAAGGACAGCAAAAGTGATTTATCTCCACCTTTAAGGCCACTTAATTTAGATGACTTTATTCGATCAAAAGCCAAGGTTGAAGCAGCTCCTGCTGCTGTAGTTCCAGTCTCTAAGTCTGGCAAGAAAGGCAAGAGGGAAGCCGAGAATGAAATCGAGAAGGCAGTGAGTGCGAAGAAGCAGAAGAGAGACGAGGGTGTGGTGCAGGCCATCCAGAAGACGAAGGCCGAAGCGAAGGCGCTGCAAAAGAAGAAGAAAGAAGAGAGCAGTAGTGAGGAGGAGTCTGACACGTCTTCATCAGAGGATGAGAAGCCTGCTCCTAAAACCAAAACTCTTGTTCCTCCCAAGAAGCAGGCCATAGTTGCTAAGAATGGTACTGCCAAGAAGGAAGAGTCCAGCAGTTCTGAATCAGAGTCTGATTCTGATGAGGATGAAAAACCTGCTACCAAAACTGGAGTTGGTGCTAAAAACGGGACAGTTGCTAAGAAAGCCGAGTCTTCTGACAGTTCAGATTCTTCAGATGATGATTCCGATGAGGATGATGTTCCTAAATCCAAAGCCACAAAATTGCCTCAAGTTGTGGCTAAGAATGGCAAGAAGAAAGAGGATTCCAGCTCCTCAGATACAGATTCTAGTGATGATTCAGAGGATGATGATGAAACTCCTGCTAAGGTTACTGCACCTGTGAAGAAGGTTGTTGCTCCTGCTGCAAAGAAGCCTGATTCAAGCAGCTCTGAAGATGATTCTGATGATGAGGATACCAGTGACGACTCATCTGATGATTCAGAGGAAGAAAAGCCAAAGAATGCTAAACCTGCTGCTAAAAGAGCTCAACCATCAAAGAGTGAGAGTGAGAGTGAAAGTGAAAGTGACTCTGATGAAGATGATGATGATGATGAGCCACAAACTAAAAACCAAAGTCCGAGGTATGTCCCTGCTAAAGCTGCCCCAAAGGAAAGTAGTTCAGAGGAGGAGAGTGATGAGGATAGTTCAGATGAAGAATCAGAAGAAGAGGCAAAGACTCCAAAGAAAAAGGACTCTGATGTAGAGATGGTAGATGCTCAATCAAAATCCGAAAAGAAAGCTGTAATAGACTGTTTCCCCTTCTTCATTATCTGCATTATCTCATATCACATATTTTGCTCTGACCTTTTGAATTTTATTACGCAGCCTAAAACCCCTGCTACTCCTGAAACGTCGTCGTCTAAGACTTTGTTTGCCGGCAATTTGTCATTCAAGATTGATCAAACAGATGTGGTAGAATTCTTCAAGGATGTTGCCCAAGTTGTTGAGGTCCGTTTCTCCTCTGATCAAGATGGACAGTTCAGGGGATATGGACATGTAGAGTTTGCCAGTGCAGAAGATGCCCAAAAGGCTCTGGAGTTGAATGGTTCAGATTTGTTGGGTCGTGCGGTGAGACTTGATCTGGCTCGTGAAAGGGGCGAAAGGGGTGCTTATACTCCTCACAGCGGCAAAGAGAGCAACTCATATCAGAAGGGAGGACAAGGTCAGTCACAGACAATATTTGTACGAGGATTTGATACAAACCAAGGGGAGGACGAGATCCGAGGTGCCCTGCAAAGTCATTTTGGTTCGTGTGGAGAGATAACACGGGTATCCATCCCAAAAGATTATGAAACTGGCGCCTCCAAGGGGTTTGTGCTTCTTTTGATGGCCTACATGGACTTCTCATCTGCGGATGCTTTCAGCAAAGCTTTGGAGCTTGATCAAAGTGATCTTGGAGATGGCTGTTATTTGAATGTCCAACCGGCCAAACCTAAAGGTGATTTTGGAGAGAGGAATTCTGGTAGTCGTTTTGATAATGGTGGCCGTCGAGGTGGCCGTCGAGGTGGTCGTTTTGAGAGTGGTGGCCGCCGTGGTGGTGGTCGTTTTGATAGTGGTGGCCGTCGTGGTGGTAGGGATTCTGGTGGTAGGCGTGGAGGCAGAGGTCCTAGCCTAGCTACTCCTGGAACAGGGAAGAAGACCACCTTCAATGACGAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

1415

Amino Acids

155.33

Weight (kDa)

4.97

Isoelectric Point (pI)

53.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7751 PF24933 440 - 494 1.8e-06 Domain of unknown function (DUF7751)
AAA PF00004 557 - 687 1.4e-36 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 712 - 748 1.7e-10 AAA+ lid domain
RRM_1 PF00076 1152 - 1219 2.6e-14 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 2134, 2689, 3064
AccB1I GGYRCC 2 cut(s) 3812, 3884
AccB7I CCANNNNNTGG 1 cut(s) 1082
AccBSI CCGCTC 1 cut(s) 684
AccII CGCG 1 cut(s) 145
AciI CCGC 7 cut(s) 75, 684, 1654, 3653, 3711, 3938, 4114
AclWI GGATC 3 cut(s) 584, 1953, 3799
AcoI YGGCCR 6 cut(s) 2003, 4011, 4069, 4081, 4111, 4144
AcsI RAATTY 8 cut(s) 978, 1058, 1078, 1741, 1977, 3397, 3503, 4042
AcuI CTGAAG 6 cut(s) 74, 246, 399, 536, 2742, 2997
AcyI GRCGYC 1 cut(s) 3885
AfaI GTAC 5 cut(s) 259, 1221, 1527, 2617, 3771
AflII CTTAAG 2 cut(s) 1112, 1133
AflIII ACRYGT 3 cut(s) 556, 2529, 3580
AhlI ACTAGT 2 cut(s) 314, 1600
AjiI CACGTC 1 cut(s) 2532
AjnI CCWGG 5 cut(s) 100, 1531, 1687, 2220, 4207
AjuI GAANNNNNNNTTGG 2 cut(s) 1799, 1831
AloI GAACNNNNNNTCC 2 cut(s) 2115, 2147
Alw21I GWGCWC 5 cut(s) 100, 139, 1570, 1885, 3073
Alw26I GTCTC 4 cut(s) 815, 2356, 2424, 3652
AlwI GGATC 3 cut(s) 584, 1953, 3799
AlwNI CAGNNNCTG 6 cut(s) 107, 473, 1001, 1733, 2333, 2657
Ama87I CYCGRG 1 cut(s) 1304
ApoI RAATTY 8 cut(s) 978, 1058, 1078, 1741, 1977, 3397, 3503, 4042
AseI ATTAAT 1 cut(s) 441
AspLEI GCGC 3 cut(s) 145, 2482, 3887
AspS9I GGNCC 8 cut(s) 545, 608, 856, 998, 1330, 1682, 3535, 4190
AsuHPI GGTGA 9 cut(s) 218, 536, 544, 691, 1457, 1547, 1591, 3667, 4037
AvaI CYCGRG 1 cut(s) 1304
AvaII GGWCC 6 cut(s) 608, 998, 1330, 1682, 3535, 4190
BaeGI GKGCMC 1 cut(s) 3817
BaeI ACNNNNGTAYC 2 cut(s) 3840, 3873
BanI GGYRCC 2 cut(s) 3812, 3884
BanII GRGCYC 2 cut(s) 139, 3073
BarI GAAGNNNNNNTAC 2 cut(s) 1203, 1235
BauI CACGAG 3 cut(s) 1464, 1884, 3672
BbsI GAAGAC 6 cut(s) 765, 1627, 2465, 2526, 2730, 4229
Bbv12I GWGCWC 5 cut(s) 100, 139, 1570, 1885, 3073
BceAI ACGGC 4 cut(s) 4056, 4068, 4101, 4131
BcgI CGANNNNNNTGC 6 cut(s) 1445, 1479, 2464, 2498, 3674, 3708
BciT130I CCWGG 5 cut(s) 102, 1533, 1689, 2222, 4209
BciVI GTATCC 4 cut(s) 783, 2159, 2992, 3868
BclI TGATCA 3 cut(s) 3484, 3550, 3967
BcoDI GTCTC 4 cut(s) 815, 2356, 2424, 3652
BcuI ACTAGT 2 cut(s) 314, 1600
BfaI CTAG 8 cut(s) 315, 927, 1601, 1914, 2120, 2874, 4194, 4199
BfmI CTRYAG 3 cut(s) 340, 1454, 2340
BfoI RGCGCY 2 cut(s) 2483, 3888
BfrI CTTAAG 2 cut(s) 1112, 1133
BfuAI ACCTGC 3 cut(s) 2134, 2689, 3064
BfuI GTATCC 4 cut(s) 783, 2159, 2992, 3868
BglI GCCNNNNNGGC 1 cut(s) 3614
BglII AGATCT 1 cut(s) 148
BlpI GCTNAGC 1 cut(s) 477
Bme1390I CCNGG 5 cut(s) 102, 1533, 1689, 2222, 4209
Bme18I GGWCC 6 cut(s) 608, 998, 1330, 1682, 3535, 4190
BmeT110I CYCGRG 1 cut(s) 1304
BmgBI CACGTC 1 cut(s) 2532
BmgT120I GGNCC 8 cut(s) 545, 608, 856, 998, 1330, 1682, 3535, 4190
BmiI GGNNCC 3 cut(s) 1993, 3814, 3886
BmrFI CCNGG 5 cut(s) 102, 1533, 1689, 2222, 4209
BmsI GCATC 6 cut(s) 280, 1780, 2011, 3289, 3595, 3931
BpiI GAAGAC 6 cut(s) 765, 1627, 2465, 2526, 2730, 4229
BplI GAGNNNNNCTC 6 cut(s) 1619, 1651, 2153, 2185, 3710, 3742
BpmI CTGGAG 4 cut(s) 84, 1592, 2715, 3641
Bpu10I CCTNAGC 1 cut(s) 2910
Bpu1102I GCTNAGC 1 cut(s) 477
BpuEI CTTGAG 3 cut(s) 374, 2094, 2799
BsaAI YACGTR 1 cut(s) 557
BsaBI GATNNNNATC 1 cut(s) 1957
BsaHI GRCGYC 1 cut(s) 3885
Bse118I RCCGGY 2 cut(s) 3461, 4009
Bse1I ACTGG 9 cut(s) 779, 988, 1699, 1762, 2348, 2698, 3003, 3594, 3886
Bse3DI GCAATG 1 cut(s) 855
Bse8I GATNNNNATC 1 cut(s) 1957
BseBI CCWGG 5 cut(s) 102, 1533, 1689, 2222, 4209
BseJI GATNNNNATC 1 cut(s) 1957
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 8 cut(s) 99, 444, 468, 822, 839, 1116, 1371, 2874
BseNI ACTGG 9 cut(s) 779, 988, 1699, 1762, 2348, 2698, 3003, 3594, 3886
BseSI GKGCMC 1 cut(s) 3817
BseYI CCCAGC 2 cut(s) 1395, 1733
BsgI GTGCAG 3 cut(s) 2465, 2904, 3618
Bsh1236I CGCG 1 cut(s) 145
BshNI GGYRCC 2 cut(s) 3812, 3884
BsiHKAI GWGCWC 5 cut(s) 100, 139, 1570, 1885, 3073
BsiHKCI CYCGRG 1 cut(s) 1304
BsiSI CCGG 2 cut(s) 3462, 4010
BslFI GGGAC 3 cut(s) 1143, 2728, 3158
BsmAI GTCTC 4 cut(s) 815, 2356, 2424, 3652
BsmBI CGTCTC 1 cut(s) 2424
BsmFI GGGAC 3 cut(s) 1143, 2728, 3158
BsmI GAATGC 1 cut(s) 3052
BsoBI CYCGRG 1 cut(s) 1304
Bsp1286I GDGCHC 6 cut(s) 100, 139, 1570, 1885, 3073, 3817
Bsp1720I GCTNAGC 1 cut(s) 477
BspACI CCGC 7 cut(s) 75, 684, 1654, 3653, 3711, 3938, 4114
BspCNI CTCAG 8 cut(s) 100, 445, 469, 823, 838, 1117, 1372, 2873
BspFNI CGCG 1 cut(s) 145
BspLI GGNNCC 3 cut(s) 1993, 3814, 3886
BspMAI CTGCAG 1 cut(s) 1458
BspMI ACCTGC 3 cut(s) 2134, 2689, 3064
BspPI GGATC 3 cut(s) 584, 1953, 3799
BspQI GCTCTTC 1 cut(s) 1181
BspT107I GGYRCC 2 cut(s) 3812, 3884
BspTI CTTAAG 2 cut(s) 1112, 1133
BsrBI CCGCTC 1 cut(s) 684
BsrDI GCAATG 1 cut(s) 855
BsrFI RCCGGY 2 cut(s) 3461, 4009
BsrI ACTGG 9 cut(s) 779, 988, 1699, 1762, 2348, 2698, 3003, 3594, 3886
BssAI RCCGGY 2 cut(s) 3461, 4009
BssNI GRCGYC 1 cut(s) 3885
BssSI CACGAG 3 cut(s) 1464, 1884, 3672
BssT1I CCWWGG 6 cut(s) 611, 1713, 2244, 2316, 3790, 3891
Bst2BI CACGAG 3 cut(s) 1464, 1884, 3672
Bst2UI CCWGG 5 cut(s) 102, 1533, 1689, 2222, 4209
Bst4CI ACNGT 7 cut(s) 119, 341, 1970, 2719, 2747, 3338, 3564
Bst6I CTCTTC 8 cut(s) 916, 1181, 1209, 1820, 2420, 2496, 2625, 3246
BstACI GRCGYC 1 cut(s) 3885
BstAFI CTTAAG 2 cut(s) 1112, 1133
BstAPI GCANNNNNTGC 3 cut(s) 2333, 2411, 3605
BstBAI YACGTR 1 cut(s) 557
BstDSI CCRYGG 1 cut(s) 4117
BstENI CCTNNNNNAGG 4 cut(s) 863, 1487, 1662, 4212
BstFNI CGCG 1 cut(s) 145
BstH2I RGCGCY 2 cut(s) 2483, 3888
BstHHI GCGC 3 cut(s) 145, 2482, 3887
BstMAI GTCTC 4 cut(s) 815, 2356, 2424, 3652
BstNI CCWGG 5 cut(s) 102, 1533, 1689, 2222, 4209
BstNSI RCATGY 5 cut(s) 371, 797, 815, 878, 3584
BstSCI CCNGG 5 cut(s) 100, 1531, 1687, 2220, 4207
BstSFI CTRYAG 3 cut(s) 340, 1454, 2340
BstSLI GKGCMC 1 cut(s) 3817
BstUI CGCG 1 cut(s) 145
BstV2I GAAGAC 6 cut(s) 765, 1627, 2465, 2526, 2730, 4229
BstX2I RGATCY 2 cut(s) 148, 3804
BstXI CCANNNNNNTGG 1 cut(s) 786
BstYI RGATCY 2 cut(s) 148, 3804
BsuI GTATCC 4 cut(s) 783, 2159, 2992, 3868
BtgI CCRYGG 1 cut(s) 4117
BtrI CACGTC 1 cut(s) 2532
BtsI GCAGTG 3 cut(s) 187, 2182, 2412
BtsIMutI CAGTG 7 cut(s) 105, 187, 512, 2182, 2412, 3010, 3601
BveI ACCTGC 3 cut(s) 2134, 2689, 3064
CaiI CAGNNNCTG 6 cut(s) 107, 473, 1001, 1733, 2333, 2657
CfoI GCGC 3 cut(s) 145, 2482, 3887
Cfr10I RCCGGY 2 cut(s) 3461, 4009
Cfr13I GGNCC 8 cut(s) 545, 608, 856, 998, 1330, 1682, 3535, 4190
CseI GACGC 1 cut(s) 1106
Csp6I GTAC 5 cut(s) 258, 1220, 1526, 2616, 3770
CviQI GTAC 5 cut(s) 258, 1220, 1526, 2616, 3770
DinI GGCGCC 1 cut(s) 3886
EaeI YGGCCR 6 cut(s) 2003, 4011, 4069, 4081, 4111, 4144
Eam1104I CTCTTC 8 cut(s) 916, 1181, 1209, 1820, 2420, 2496, 2625, 3246
EarI CTCTTC 8 cut(s) 916, 1181, 1209, 1820, 2420, 2496, 2625, 3246
Ecl136II GAGCTC 2 cut(s) 137, 3071
Eco130I CCWWGG 6 cut(s) 611, 1713, 2244, 2316, 3790, 3891
Eco147I AGGCCT 2 cut(s) 643, 2215
Eco24I GRGCYC 2 cut(s) 139, 3073
Eco47I GGWCC 6 cut(s) 608, 998, 1330, 1682, 3535, 4190
Eco53kI GAGCTC 2 cut(s) 137, 3071
Eco57I CTGAAG 6 cut(s) 74, 246, 399, 536, 2742, 2997
Eco88I CYCGRG 1 cut(s) 1304
EcoICRI GAGCTC 2 cut(s) 137, 3071
EcoNI CCTNNNNNAGG 4 cut(s) 863, 1487, 1662, 4212
EcoO109I RGGNCCY 3 cut(s) 856, 998, 4190
EcoRI GAATTC 3 cut(s) 1977, 3503, 4042
EcoRII CCWGG 5 cut(s) 100, 1531, 1687, 2220, 4207
EcoT14I CCWWGG 6 cut(s) 611, 1713, 2244, 2316, 3790, 3891
EcoT22I ATGCAT 1 cut(s) 1328
EcoT38I GRGCYC 2 cut(s) 139, 3073
EgeI GGCGCC 1 cut(s) 3886
EheI GGCGCC 1 cut(s) 3886
ErhI CCWWGG 6 cut(s) 611, 1713, 2244, 2316, 3790, 3891
Esp3I CGTCTC 1 cut(s) 2424
FalI AAGNNNNNCTT 6 cut(s) 1206, 1238, 3679, 3711, 4214, 4246
FaqI GGGAC 3 cut(s) 1143, 2728, 3158
FauI CCCGC 1 cut(s) 68
FbaI TGATCA 3 cut(s) 3484, 3550, 3967
FriOI GRGCYC 2 cut(s) 139, 3073
FspBI CTAG 8 cut(s) 315, 927, 1601, 1914, 2120, 2874, 4194, 4199
GlaI GCGC 3 cut(s) 144, 2481, 3886
GsaI CCCAGC 2 cut(s) 1399, 1737
GsuI CTGGAG 4 cut(s) 84, 1592, 2715, 3641
HaeII RGCGCY 2 cut(s) 2483, 3888
HapII CCGG 2 cut(s) 3462, 4010
HgaI GACGC 1 cut(s) 1106
HhaI GCGC 3 cut(s) 145, 2482, 3887
Hin1I GRCGYC 1 cut(s) 3885
Hin6I GCGC 3 cut(s) 143, 2480, 3885
HinP1I GCGC 3 cut(s) 143, 2480, 3885
HincII GTYRAC 1 cut(s) 659
HindII GTYRAC 1 cut(s) 659
HindIII AAGCTT 3 cut(s) 395, 1832, 3954
HpaII CCGG 2 cut(s) 3462, 4010
HphI GGTGA 9 cut(s) 218, 536, 544, 691, 1457, 1547, 1591, 3667, 4037
Hpy166II GTNNAC 4 cut(s) 39, 258, 659, 1019
Hpy8I GTNNAC 4 cut(s) 39, 258, 659, 1019
Hpy99I CGWCG 6 cut(s) 2037, 3442, 3445, 4077, 4089, 4152
HpyCH4III ACNGT 7 cut(s) 119, 341, 1970, 2719, 2747, 3338, 3564
HpyCH4IV ACGT 4 cut(s) 556, 661, 2531, 3437
HpySE526I ACGT 4 cut(s) 556, 661, 2531, 3437
Hsp92I GRCGYC 1 cut(s) 3885
HspAI GCGC 3 cut(s) 143, 2480, 3885
KasI GGCGCC 1 cut(s) 3884
KroI GCCGGC 1 cut(s) 3461
KroNI GCCGGC 1 cut(s) 3463
Ksp22I TGATCA 3 cut(s) 3484, 3550, 3967
LguI GCTCTTC 1 cut(s) 1181
LweI GCATC 6 cut(s) 280, 1780, 2011, 3289, 3595, 3931
MaeI CTAG 8 cut(s) 315, 927, 1601, 1914, 2120, 2874, 4194, 4199
MaeII ACGT 4 cut(s) 556, 661, 2531, 3437
MaeIII GTNAC 6 cut(s) 1445, 1505, 2914, 3005, 3110, 3753
MbiI CCGCTC 1 cut(s) 684
MflI RGATCY 2 cut(s) 148, 3804
MhlI GDGCHC 6 cut(s) 100, 139, 1570, 1885, 3073, 3817
Mly113I GGCGCC 1 cut(s) 3885
MmeI TCCRAC 1 cut(s) 4030
Mph1103I ATGCAT 1 cut(s) 1328
MroNI GCCGGC 1 cut(s) 3461
MseI TTAA 7 cut(s) 441, 1010, 1113, 1134, 1440, 2276, 2286
MslI CAYNNNNRTG 2 cut(s) 816, 1777
MspA1I CMGCKG 1 cut(s) 3711
MspCI CTTAAG 2 cut(s) 1112, 1133
MspI CCGG 2 cut(s) 3462, 4010
MspR9I CCNGG 5 cut(s) 102, 1533, 1689, 2222, 4209
Mva1269I GAATGC 1 cut(s) 3052
MvaI CCWGG 5 cut(s) 102, 1533, 1689, 2222, 4209
MvnI CGCG 1 cut(s) 145
NaeI GCCGGC 1 cut(s) 3463
NarI GGCGCC 1 cut(s) 3885
NgoMIV GCCGGC 1 cut(s) 3461
NlaIV GGNNCC 3 cut(s) 1993, 3814, 3886
NmeAIII GCCGAG 2 cut(s) 2410, 2758
NmuCI GTSAC 4 cut(s) 1445, 3005, 3110, 3753
NsiI ATGCAT 1 cut(s) 1328
NspI RCATGY 5 cut(s) 371, 797, 815, 878, 3584
PaeI GCATGC 2 cut(s) 371, 878
PaeR7I CTCGAG 1 cut(s) 1304
PceI AGGCCT 2 cut(s) 643, 2215
PciI ACATGT 1 cut(s) 3580
PciSI GCTCTTC 1 cut(s) 1181
PcsI WCGNNNNNNNCGW 2 cut(s) 4081, 4093
PctI GAATGC 1 cut(s) 3052
PdiI GCCGGC 1 cut(s) 3463
PflFI GACNNNGTC 2 cut(s) 959, 3747
PflMI CCANNNNNTGG 1 cut(s) 1082
PfoI TCCNGGA 3 cut(s) 1687, 2220, 4207
PluTI GGCGCC 1 cut(s) 3888
Ppu21I YACGTR 1 cut(s) 557
PpuMI RGGWCCY 2 cut(s) 998, 4190
PscI ACATGT 1 cut(s) 3580
PshBI ATTAAT 1 cut(s) 441
Psp124BI GAGCTC 2 cut(s) 139, 3073
Psp5II RGGWCCY 2 cut(s) 998, 4190
Psp6I CCWGG 5 cut(s) 100, 1531, 1687, 2220, 4207
PspFI CCCAGC 2 cut(s) 1395, 1733
PspGI CCWGG 5 cut(s) 100, 1531, 1687, 2220, 4207
PspN4I GGNNCC 3 cut(s) 1993, 3814, 3886
PspPI GGNCC 8 cut(s) 545, 608, 856, 998, 1330, 1682, 3535, 4190
PspPPI RGGWCCY 2 cut(s) 998, 4190
PstI CTGCAG 1 cut(s) 1458
PstNI CAGNNNCTG 6 cut(s) 107, 473, 1001, 1733, 2333, 2657
PsuI RGATCY 2 cut(s) 148, 3804
PsyI GACNNNGTC 2 cut(s) 959, 3747
RsaI GTAC 5 cut(s) 259, 1221, 1527, 2617, 3771
RsaNI GTAC 5 cut(s) 258, 1220, 1526, 2616, 3770
RseI CAYNNNNRTG 2 cut(s) 816, 1777
SacI GAGCTC 2 cut(s) 139, 3073
SapI GCTCTTC 1 cut(s) 1181
SaqAI TTAA 7 cut(s) 441, 1010, 1113, 1134, 1440, 2276, 2286
Sau96I GGNCC 8 cut(s) 545, 608, 856, 998, 1330, 1682, 3535, 4190
ScrFI CCNGG 5 cut(s) 102, 1533, 1689, 2222, 4209
SduI GDGCHC 6 cut(s) 100, 139, 1570, 1885, 3073, 3817
SfaNI GCATC 6 cut(s) 280, 1780, 2011, 3289, 3595, 3931
SfcI CTRYAG 3 cut(s) 340, 1454, 2340
SfoI GGCGCC 1 cut(s) 3886
Sfr274I CTCGAG 1 cut(s) 1304
SinI GGWCC 6 cut(s) 608, 998, 1330, 1682, 3535, 4190
SlaI CTCGAG 1 cut(s) 1304
SmiMI CAYNNNNRTG 2 cut(s) 816, 1777
SmlI CTYRAG 6 cut(s) 389, 1112, 1133, 1304, 2109, 2814
SmoI CTYRAG 6 cut(s) 389, 1112, 1133, 1304, 2109, 2814
SpeI ACTAGT 2 cut(s) 314, 1600
SphI GCATGC 2 cut(s) 371, 878
SseBI AGGCCT 2 cut(s) 643, 2215
SsiI CCGC 7 cut(s) 75, 684, 1654, 3653, 3711, 3938, 4114
SspDI GGCGCC 1 cut(s) 3884
SspI AATATT 1 cut(s) 3765
SspMI CTAG 8 cut(s) 315, 927, 1601, 1914, 2120, 2874, 4194, 4199
SstI GAGCTC 2 cut(s) 139, 3073
StuI AGGCCT 2 cut(s) 643, 2215
StyD4I CCNGG 5 cut(s) 100, 1531, 1687, 2220, 4207
StyI CCWWGG 6 cut(s) 611, 1713, 2244, 2316, 3790, 3891
TaaI ACNGT 7 cut(s) 119, 341, 1970, 2719, 2747, 3338, 3564
TaiI ACGT 4 cut(s) 559, 664, 2534, 3440
TaqI TCGA 8 cut(s) 691, 807, 1305, 1857, 2305, 2397, 4075, 4087
TatI WGTACW 1 cut(s) 257
TauI GCSGC 3 cut(s) 1657, 3714, 4116
Tru1I TTAA 7 cut(s) 441, 1010, 1113, 1134, 1440, 2276, 2286
Tru9I TTAA 7 cut(s) 441, 1010, 1113, 1134, 1440, 2276, 2286
TscAI CASTG 7 cut(s) 112, 187, 519, 2182, 2412, 3010, 3601
TseFI GTSAC 4 cut(s) 1445, 3005, 3110, 3753
Tsp45I GTSAC 4 cut(s) 1445, 3005, 3110, 3753
TspGWI ACGGA 4 cut(s) 236, 851, 1426, 3527
TspRI CASTG 7 cut(s) 112, 187, 519, 2182, 2412, 3010, 3601
Tth111I GACNNNGTC 2 cut(s) 959, 3747
Van91I CCANNNNNTGG 1 cut(s) 1082
Vha464I CTTAAG 2 cut(s) 1112, 1133
VpaK11BI GGWCC 6 cut(s) 608, 998, 1330, 1682, 3535, 4190
VspI ATTAAT 1 cut(s) 441
XagI CCTNNNNNAGG 4 cut(s) 863, 1487, 1662, 4212
XapI RAATTY 8 cut(s) 978, 1058, 1078, 1741, 1977, 3397, 3503, 4042
XbaI TCTAGA 1 cut(s) 2119
XceI RCATGY 5 cut(s) 371, 797, 815, 878, 3584
XcmI CCANNNNNNNNNTGG 1 cut(s) 783
XhoI CTCGAG 1 cut(s) 1304
XspI CTAG 8 cut(s) 315, 927, 1601, 1914, 2120, 2874, 4194, 4199
Zsp2I ATGCAT 1 cut(s) 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.