Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004154

Physical Location & Seq

Genomic Coordinates Forward (+)

88756 .. 98190

Gene Structure 9435 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1001500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3027 bp

ATGATGCATGCAAGGAGAATAAAGTGTAAAAATCAGAGATGGGTACTTCAAATGTCCAAATATTCAACAACGCCTACCCACACTGTTCATGGTTCTTCGCAATCTTTAGGTCATAGTACAGTTTCGGGAAACCACTGTTTTCATGGTAGTTTTATTAGAAGCCGTCTCGTTGATTCATTCACGTTGCGTAGTGTTGCTCCTGGTTATAGCTGCACTGGTTTGCATGTAAGGTCTAGTCCATGTTTGAAGGGTAGCCAGTTACGTGCCTACAGTTCTGAAGGTGATGGAAGTAATGCAAGTGAGGATAATCAAACACCAGTGAAAGGTGATGCTGATCTTGATAAAGGGAGGACTTCTCAAGAAAAGGCTAAGGAAGATGGTAGGAATTTTGATGCACACGCTCTACTTGGGGAACAAGATCAAAAGGAGTGGCTTAATAGTCAAAAGATTACCATTGAGAGTAAAAAGAAAGAGTCACCTTTTTCAACTAAACGAGAGAAGTTCAAAAATGAGTTTTTGAGGAGGATTGTTCCGTGGGAGAAATTAACTGTGTCATGGGATACATTTCCCTACTACATTCATGAACCTACCAAAAACCTTTTGGTGGAATGTGCTGCTTCCCATTTGAAGCATAAAAAGTTTACTTCAACTTATGGTTCTCGTTTGACATCTTCAAATGGAAGGATACTGCTTCAGAGTTCACCAGGCACTGAACTTTATCGTGAAAGATTAGTTAGAGCACTCACACAAGATCTCAAAGTTCCCCTCTTGGTGCTAGACAGCAGTGTACTTGCTCCTTATGATTTTGGTGACGACTGTGAATCTGAATCGGATGATGATGGTGTGGAGGAGAATACTTCTGATTCAGAGATTGAGGATGAAAATGCTGCGAGTAACGAAGAAGATTGGACTAGTAGTAACGAGGCAAAATCAGATTCTAGTGATAAAGATGAAGATGATGTGCATGCCAGAGCTGAAGCAGCCCTCAAGAAGCTTGTTCCTATTGAAGACTTTGCAAAGATGGTATCTGGGGAAACTGAGAGTTCATCCGAATCCTCAAAATCAGAAACTGCCGAGTCTTCTGATAAATGCAAACGACCGCTAAAGAAAGGAGATCGAGTGAAGTATATCGGGCCTTCTATACATGTTGAAGCTGATAACAGGCCTTTACCAAAGGGTCAACGTGGGGAGGTATATGAGGTGAGTGGAGATCGAGTTGCTGTTATTTTGGATGTTAAACAGAAGGCAGGAACTGAAGTGGATAAAGAGGAAAAAGATGCAGAGCAACCTGCTGATCCACCAGTTTATTGGATACATGCTAAGGAAATTGAACATATTCCTGACCCGCAGACTGAGGATTGTTATTTTGCACTGGAGGCCTTATGTGAGGTCTTACATGCCAAGCAACCTCTTATTGTATATTTTCCAGACTCTTCTCAATGGATGTCTAGGGCAGTTCCTAAGTCAGATCGCAAGGAGTTTGTCGCTAAGGTGCAAGAAGTTTTTGATGAGTTATCAGGTCCTGTTGTTTTGATATGCGGGCAGAACAAGGTTGAATCAGGATCAAAGGAGAAAGAGAAATTTACAATGATACTTCCAAATTTGGGGCGTCTTGCAAAGCTGCCTCTCTCTCTTAAGCGTCTTACAGAGGGACTTAAAGGAACAAAGAGATCAGATGACAATGAAATATATAAGCTATTCAAAAATGTTTTCTGCGTATATCCGCCAAAGGAGGAAGAGGAGCTTCGAATATTCAATAAACAGATTGAAGAAGATGGAAGAATTGTGATAGCAAGAAGCAATTTAAATGAATTACATGAGGTTCTTGAGGAAAATGAGCTCTCATGCATGGACTTATTGCTAGTAGATACCAATGGTGTAATCTTGACAAAGAGAACTGAGAAAGTAGTTGGCTGGGCAAAAAACCATTACTTGTCATCATGCCTTCTTCCTTACGTGAAAGGTGAAAGATTGCATCTGCCACGTGAAAGCCTTGAAATAGCAATTTCACGCTTGAAGGAGCAAGAAACTTTGTCTCGGAAGCCTTCTCAAAATTTGAAGAACATGGCAAAGGATGAATATGAGAGCAACTTTGTTTCAGCTGTAGTACCTCCTGGTGAAATTGGTGTTAGGTTTGATGATGTAGGTGCTCTGGAGGACGTGAAGAGGGCGCTGAATGAACTTGTTATTCTTCCAATGAGGAGACCGGAGCTTTTCTCTCATGGAAACTTATTGCGGCCTTGCAAAGGAATATTACTTTTTGGGCCTCCTGGAACTGGGAAGACCCTTCTTGCCAAGGCACTTGCAACAGAAGCTGGGGCAAACTTTATCAGCATAACTGGTTCTACTCTAACATCAAAGTGGTTTGGAGATGCTGAAAAGCTGACCAAAGCTTTGTTCTCCTTTGCTAGCAAGCTTGCTCCCGTCATAATATTTGTTGACGAGGTTGATAGTTTGCTTGGTGCTCGTGGTGGTTCTTTTGAGCATGAGGCAACTAGAAGAATGAGAAATGAGTTCATGGCAGCTTGGGATGGATTGAGGTCCAAAGACAGTCAAAGAATCCTTATTCTTGGGGCCACAAATCGGCCATTTGATCTTGATGATGCTGTCATTCGTCGGTTACCAAGAAGATTGGTTACCGTGGGTCTGATATACGTTGACTTACCTGATGTTGAAAATCGTAAGAAGATATTGAGTATATTTCTTGCTCAAGAGAATCTAGCACCTGGTTTCCAATTTGACAAACTTTCAGAAGCAACAGAGGGATATTCTGGCAGTGATTTGAAGAACCTCTGTATTGCTGCTGCATATAGACCTGTCCAGGAGCTTTTAGAAGAAGAAAAGAAGGACATCAAAAGTGACGCAGCTACAGTGTTGAGGCCTCTTAATTTAGATGATTTTATTCAATCAAAAGCCAAGGTTGGGCCATCAGTTTCCTATGATGCTTCAAGCATGAACGAACTTAGGAAATGGAACGAACAATATGGAGAAGGTGGAACCAGAAGAAAATCACCATTTGGTTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA

Protein Analysis

1009

Amino Acids

112.93

Weight (kDa)

5.83

Isoelectric Point (pI)

52.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	751 - 878	1.6e-35	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	911 - 951	1.2e-10	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004154)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50140 AT1G50140 AT1G50140 AT1G50140 AT1G50140 AT3G19740
fragaria_vesca	FvH4_4g36860 FvH4_4g36860 FvH4_4g36860
malus_domestica	MD13G1006500.v1.1 MD16G1001500.v1.1
prunus_persica	Prupe.1G346700_v2.0.a1 Prupe.1G346700_v2.0.a1 Prupe.1G346700_v2.0.a1 Prupe.1G346700_v2.0.a1 Prupe.1G346700_v2.0.a1 Prupe.1G346700_v2.0.a1
pyrus_communis	pycom13g00540 pycom16g00410
rosa_chinensis	RchiOBHm_Chr4g0446611
rosa_laevigata	RLG00000005645
rosa_multiflora	Rmu_sc0036387.1_g000001 Rmu_ssc0000144.1_g000003
rosa_roxburghii	Rroxscaffold_5G00387010
rosa_rugosa	Rorug04G0372800 Rorug04G0372900 Rorug04G0373000 Rorug04G0373100
rosa_samantha	Rh4AG434400 Rh4BG443600 Rh4CG460900 Rh4DG442400
rosa_wichuraiana	Rw4G037140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1297
AciI	CCGC	5 cut(s)	1100, 1346, 1539, 1724, 2236
AclWI	GGATC	2 cut(s)	1289, 1570
AcoI	YGGCCR	1 cut(s)	2585
AcsI	RAATTY	4 cut(s)	385, 1580, 1600, 2053
AcuI	CTGAAG	4 cut(s)	297, 677, 996, 1275
AcvI	CACGTG	1 cut(s)	1985
AcyI	GRCGYC	1 cut(s)	1609
AfaI	GTAC	4 cut(s)	45, 118, 789, 2109
AfiI	CCNNNNNNNGG	7 cut(s)	323, 604, 1604, 2246, 2276, 2583, 2922
AflII	CTTAAG	1 cut(s)	1634
AflIII	ACRYGT	1 cut(s)	1144
AhlI	ACTAGT	1 cut(s)	911
AjiI	CACGTC	1 cut(s)	2161
AjnI	CCWGG	6 cut(s)	199, 703, 2113, 2269, 2725, 2820
AjuI	GAANNNNNNNTTGG	2 cut(s)	2381, 2413
Alw21I	GWGCWC	4 cut(s)	742, 1842, 2152, 2467
Alw26I	GTCTC	3 cut(s)	170, 2040, 2197
AlwI	GGATC	2 cut(s)	1289, 1570
AlwNI	CAGNNNCTG	5 cut(s)	710, 1070, 1253, 1523, 2315
AoxI	GGCC	9 cut(s)	1133, 1163, 1377, 2237, 2264, 2574, 2585, 2879, 2924
ApeKI	GCWGC	9 cut(s)	210, 614, 887, 980, 1621, 2522, 2801, 2804, 2864
ApoI	RAATTY	4 cut(s)	385, 1580, 1600, 2053
ArsI	GACNNNNNNTTYG	2 cut(s)	2377, 2409
Asp700I	GAANNNNTTC	1 cut(s)	1335
AspLEI	GCGC	1 cut(s)	2173
AspS9I	GGNCC	6 cut(s)	1133, 1520, 2264, 2541, 2574, 2924
AsuHPI	GGTGA	9 cut(s)	293, 338, 468, 693, 821, 1213, 1976, 2129, 3003
AsuII	TTCGAA	1 cut(s)	1747
AsuNHI	GCTAGC	1 cut(s)	2408
AvaII	GGWCC	2 cut(s)	1520, 2541
BanII	GRGCYC	1 cut(s)	1842
BarI	GAAGNNNNNNTAC	2 cut(s)	2620, 2652
BauI	CACGAG	1 cut(s)	2466
BbrPI	CACGTG	1 cut(s)	1985
BbsI	GAAGAC	3 cut(s)	1014, 1071, 2288
Bbv12I	GWGCWC	4 cut(s)	742, 1842, 2152, 2467
BbvI	GCAGC	9 cut(s)	197, 601, 874, 992, 1608, 2534, 2788, 2791, 2876
BccI	CCATC	8 cut(s)	33, 278, 371, 833, 1015, 1769, 2525, 2935
BceAI	ACGGC	1 cut(s)	147
BciT130I	CCWGG	6 cut(s)	201, 705, 2115, 2271, 2727, 2822
BciVI	GTATCC	3 cut(s)	553, 678, 1305
BcoDI	GTCTC	3 cut(s)	170, 2040, 2197
BcuI	ACTAGT	1 cut(s)	911
BfaI	CTAG	9 cut(s)	234, 776, 912, 939, 1449, 1862, 2409, 2496, 2720
BfmI	CTRYAG	3 cut(s)	268, 2103, 2868
BfoI	RGCGCY	1 cut(s)	2174
BfrI	CTTAAG	1 cut(s)	1634
BfuAI	ACCTGC	1 cut(s)	1297
BfuI	GTATCC	3 cut(s)	553, 678, 1305
BglII	AGATCT	1 cut(s)	751
Bme1390I	CCNGG	6 cut(s)	201, 705, 2115, 2271, 2727, 2822
Bme18I	GGWCC	2 cut(s)	1520, 2541
BmgBI	CACGTC	1 cut(s)	2161
BmgT120I	GGNCC	6 cut(s)	1133, 1520, 2264, 2541, 2574, 2924
BmiI	GGNNCC	2 cut(s)	2575, 2998
BmrFI	CCNGG	6 cut(s)	201, 705, 2115, 2271, 2727, 2822
BmrI	ACTGGG	1 cut(s)	2286
BmsI	GCATC	7 cut(s)	319, 382, 1267, 1984, 2362, 2593, 2932
BmtI	GCTAGC	1 cut(s)	2412
BmuI	ACTGGG	1 cut(s)	2286
BpiI	GAAGAC	3 cut(s)	1014, 1071, 2288
BplI	GAGNNNNNCTC	4 cut(s)	340, 372, 2201, 2233
BpmI	CTGGAG	2 cut(s)	1394, 2174
Bpu10I	CCTNAGC	3 cut(s)	369, 1320, 1488
Bpu14I	TTCGAA	1 cut(s)	1747
BpuEI	CTTGAG	4 cut(s)	342, 971, 1847, 2694
BsaAI	YACGTR	3 cut(s)	263, 1957, 1985
BsaBI	GATNNNNATC	1 cut(s)	333
BsaHI	GRCGYC	1 cut(s)	1609
BsaI	GGTCTC	1 cut(s)	2197
BsaJI	CCNNGG	4 cut(s)	533, 2295, 2640, 2916
BsaWI	WCCGGW	1 cut(s)	2206
BsaXI	ACNNNNNCTCC	4 cut(s)	1104, 1134, 1200, 1230
Bsc4I	CCNNNNNNNGG	7 cut(s)	323, 604, 1604, 2246, 2276, 2583, 2922
Bse1I	ACTGG	7 cut(s)	220, 256, 317, 1301, 1377, 2281, 2344
Bse8I	GATNNNNATC	1 cut(s)	333
BseBI	CCWGG	6 cut(s)	201, 705, 2115, 2271, 2727, 2822
BseDI	CCNNGG	4 cut(s)	533, 2295, 2640, 2916
BseGI	GGATG	7 cut(s)	838, 883, 1046, 1237, 1449, 2080, 2536
BseJI	GATNNNNATC	1 cut(s)	333
BseLI	CCNNNNNNNGG	7 cut(s)	323, 604, 1604, 2246, 2276, 2583, 2922
BseMII	CTCAG	3 cut(s)	1029, 1344, 1890
BseNI	ACTGG	7 cut(s)	220, 256, 317, 1301, 1377, 2281, 2344
BseRI	GAGGAG	4 cut(s)	535, 863, 1754, 2215
BseXI	GCAGC	9 cut(s)	197, 601, 874, 992, 1608, 2534, 2788, 2791, 2876
BseYI	CCCAGC	2 cut(s)	1914, 2315
BsgI	GTGCAG	1 cut(s)	196
Bsh1285I	CGRYCG	1 cut(s)	1100
BshFI	GGCC	9 cut(s)	1135, 1165, 1379, 2239, 2266, 2576, 2587, 2881, 2926
BsiEI	CGRYCG	1 cut(s)	1100
BsiHKAI	GWGCWC	4 cut(s)	742, 1842, 2152, 2467
BsiSI	CCGG	1 cut(s)	2207
BslFI	GGGAC	1 cut(s)	1665
BslI	CCNNNNNNNGG	7 cut(s)	323, 604, 1604, 2246, 2276, 2583, 2922
BsmAI	GTCTC	3 cut(s)	170, 2040, 2197
BsmBI	CGTCTC	1 cut(s)	170
BsmFI	GGGAC	1 cut(s)	1665
BsnI	GGCC	9 cut(s)	1135, 1165, 1379, 2239, 2266, 2576, 2587, 2881, 2926
Bso31I	GGTCTC	1 cut(s)	2197
Bsp119I	TTCGAA	1 cut(s)	1747
Bsp1286I	GDGCHC	4 cut(s)	742, 1842, 2152, 2467
BspACI	CCGC	5 cut(s)	1100, 1346, 1539, 1724, 2236
BspANI	GGCC	9 cut(s)	1135, 1165, 1379, 2239, 2266, 2576, 2587, 2881, 2926
BspCNI	CTCAG	3 cut(s)	1030, 1345, 1891
BspHI	TCATGA	1 cut(s)	580
BspLI	GGNNCC	2 cut(s)	2575, 2998
BspMI	ACCTGC	1 cut(s)	1297
BspOI	GCTAGC	1 cut(s)	2412
BspPI	GGATC	2 cut(s)	1289, 1570
BspT104I	TTCGAA	1 cut(s)	1747
BspTI	CTTAAG	1 cut(s)	1634
BspTNI	GGTCTC	1 cut(s)	2197
BsrI	ACTGG	7 cut(s)	220, 256, 317, 1301, 1377, 2281, 2344
BssECI	CCNNGG	4 cut(s)	533, 2295, 2640, 2916
BssNI	GRCGYC	1 cut(s)	1609
BssSI	CACGAG	1 cut(s)	2466
BssT1I	CCWWGG	2 cut(s)	2295, 2916
Bst2BI	CACGAG	1 cut(s)	2466
Bst2UI	CCWGG	6 cut(s)	201, 705, 2115, 2271, 2727, 2822
Bst4CI	ACNGT	9 cut(s)	85, 121, 137, 272, 550, 818, 2552, 2641, 2872
Bst6I	CTCTTC	3 cut(s)	1438, 1731, 2159
BstACI	GRCGYC	1 cut(s)	1609
BstAFI	CTTAAG	1 cut(s)	1634
BstBAI	YACGTR	3 cut(s)	263, 1957, 1985
BstBI	TTCGAA	1 cut(s)	1747
BstC8I	GCNNGC	6 cut(s)	9, 966, 1541, 2410, 2414, 2418
BstDEI	CTNAG	8 cut(s)	369, 1038, 1320, 1353, 1461, 1488, 1899, 2963
BstDSI	CCRYGG	2 cut(s)	533, 2640
BstEII	GGTNACC	2 cut(s)	2619, 2635
BstENI	CCTNNNNNAGG	1 cut(s)	2244
BstF5I	GGATG	7 cut(s)	838, 883, 1046, 1237, 1449, 2080, 2536
BstH2I	RGCGCY	1 cut(s)	2174
BstHHI	GCGC	1 cut(s)	2173
BstMAI	GTCTC	3 cut(s)	170, 2040, 2197
BstMCI	CGRYCG	1 cut(s)	1100
BstMWI	GCNNNNNNNGC	3 cut(s)	980, 1376, 2312
BstNI	CCWGG	6 cut(s)	201, 705, 2115, 2271, 2727, 2822
BstNSI	RCATGY	6 cut(s)	11, 227, 968, 1148, 1319, 1400
BstPI	GGTNACC	2 cut(s)	2619, 2635
BstSCI	CCNGG	6 cut(s)	199, 703, 2113, 2269, 2725, 2820
BstSFI	CTRYAG	3 cut(s)	268, 2103, 2868
BstV1I	GCAGC	9 cut(s)	197, 601, 874, 992, 1608, 2534, 2788, 2791, 2876
BstV2I	GAAGAC	3 cut(s)	1014, 1071, 2288
BstX2I	RGATCY	1 cut(s)	751
BstXI	CCANNNNNNTGG	1 cut(s)	1308
BstYI	RGATCY	1 cut(s)	751
BsuI	GTATCC	3 cut(s)	553, 678, 1305
BsuRI	GGCC	9 cut(s)	1135, 1165, 1379, 2239, 2266, 2576, 2587, 2881, 2926
BtgI	CCRYGG	2 cut(s)	533, 2640
BtrI	CACGTC	1 cut(s)	2161
BtsCI	GGATG	7 cut(s)	838, 883, 1046, 1237, 1449, 2080, 2536
BtsI	GCAGTG	2 cut(s)	790, 2782
BtsIMutI	CAGTG	9 cut(s)	81, 133, 213, 324, 708, 790, 1370, 2782, 2877
BveI	ACCTGC	1 cut(s)	1297
Cac8I	GCNNGC	6 cut(s)	9, 966, 1541, 2410, 2414, 2418
CaiI	CAGNNNCTG	5 cut(s)	710, 1070, 1253, 1523, 2315
CciI	TCATGA	1 cut(s)	580
CfoI	GCGC	1 cut(s)	2173
Cfr13I	GGNCC	6 cut(s)	1133, 1520, 2264, 2541, 2574, 2924
CseI	GACGC	3 cut(s)	1598, 1628, 2870
CsiI	ACCWGGT	1 cut(s)	2725
Csp6I	GTAC	4 cut(s)	44, 117, 788, 2108
CspCI	CAANNNNNGTGG	2 cut(s)	410, 445
CviQI	GTAC	4 cut(s)	44, 117, 788, 2108
DdeI	CTNAG	8 cut(s)	369, 1038, 1320, 1353, 1461, 1488, 1899, 2963
DraI	TTTAAA	1 cut(s)	1806
EaeI	YGGCCR	1 cut(s)	2585
Eam1104I	CTCTTC	3 cut(s)	1438, 1731, 2159
EarI	CTCTTC	3 cut(s)	1438, 1731, 2159
EciI	GGCGGA	1 cut(s)	1713
Ecl136II	GAGCTC	1 cut(s)	1840
Eco130I	CCWWGG	2 cut(s)	2295, 2916
Eco147I	AGGCCT	3 cut(s)	1165, 1379, 2881
Eco24I	GRGCYC	1 cut(s)	1842
Eco31I	GGTCTC	1 cut(s)	2197
Eco47I	GGWCC	2 cut(s)	1520, 2541
Eco53kI	GAGCTC	1 cut(s)	1840
Eco57I	CTGAAG	4 cut(s)	297, 677, 996, 1275
Eco72I	CACGTG	1 cut(s)	1985
Eco91I	GGTNACC	2 cut(s)	2619, 2635
EcoICRI	GAGCTC	1 cut(s)	1840
EcoNI	CCTNNNNNAGG	1 cut(s)	2244
EcoO109I	RGGNCCY	1 cut(s)	1520
EcoO65I	GGTNACC	2 cut(s)	2619, 2635
EcoRII	CCWGG	6 cut(s)	199, 703, 2113, 2269, 2725, 2820
EcoT14I	CCWWGG	2 cut(s)	2295, 2916
EcoT22I	ATGCAT	2 cut(s)	9, 1850
EcoT38I	GRGCYC	1 cut(s)	1842
ErhI	CCWWGG	2 cut(s)	2295, 2916
Esp3I	CGTCTC	1 cut(s)	170
FalI	AAGNNNNNCTT	2 cut(s)	1728, 1760
FaqI	GGGAC	1 cut(s)	1665
FauI	CCCGC	2 cut(s)	1353, 1532
FokI	GGATG	7 cut(s)	845, 890, 1033, 1244, 1456, 2087, 2543
FriOI	GRGCYC	1 cut(s)	1842
FspBI	CTAG	9 cut(s)	234, 776, 912, 939, 1449, 1862, 2409, 2496, 2720
GlaI	GCGC	1 cut(s)	2172
GsaI	CCCAGC	2 cut(s)	1918, 2319
GsuI	CTGGAG	2 cut(s)	1394, 2174
HaeII	RGCGCY	1 cut(s)	2174
HaeIII	GGCC	9 cut(s)	1135, 1165, 1379, 2239, 2266, 2576, 2587, 2881, 2926
HapII	CCGG	1 cut(s)	2207
HgaI	GACGC	3 cut(s)	1598, 1628, 2870
HhaI	GCGC	1 cut(s)	2173
Hin1I	GRCGYC	1 cut(s)	1609
Hin6I	GCGC	1 cut(s)	2171
HinP1I	GCGC	1 cut(s)	2171
HincII	GTYRAC	3 cut(s)	1181, 2440, 2659
HindII	GTYRAC	3 cut(s)	1181, 2440, 2659
HindIII	AAGCTT	3 cut(s)	992, 2391, 2414
HpaII	CCGG	1 cut(s)	2207
HphI	GGTGA	9 cut(s)	293, 338, 468, 693, 821, 1213, 1976, 2129, 3003
Hpy166II	GTNNAC	6 cut(s)	642, 701, 788, 1181, 2440, 2659
Hpy8I	GTNNAC	6 cut(s)	642, 701, 788, 1181, 2440, 2659
Hpy99I	CGWCG	1 cut(s)	2619
HpyCH4III	ACNGT	9 cut(s)	85, 121, 137, 272, 550, 818, 2552, 2641, 2872
HpyCH4IV	ACGT	7 cut(s)	182, 262, 1183, 1956, 1984, 2160, 2655
HpyF10VI	GCNNNNNNNGC	3 cut(s)	980, 1376, 2312
HpyF3I	CTNAG	8 cut(s)	369, 1038, 1320, 1353, 1461, 1488, 1899, 2963
HpySE526I	ACGT	7 cut(s)	182, 262, 1183, 1956, 1984, 2160, 2655
Hsp92I	GRCGYC	1 cut(s)	1609
HspAI	GCGC	1 cut(s)	2171
LmnI	GCTCC	7 cut(s)	202, 799, 1741, 2020, 2209, 2425, 2824
Lsp1109I	GCAGC	9 cut(s)	197, 601, 874, 992, 1608, 2534, 2788, 2791, 2876
LweI	GCATC	7 cut(s)	319, 382, 1267, 1984, 2362, 2593, 2932
MabI	ACCWGGT	1 cut(s)	2725
MaeI	CTAG	9 cut(s)	234, 776, 912, 939, 1449, 1862, 2409, 2496, 2720
MaeII	ACGT	7 cut(s)	182, 262, 1183, 1956, 1984, 2160, 2655
MaeIII	GTNAC	8 cut(s)	258, 474, 809, 893, 917, 2619, 2635, 2858
MflI	RGATCY	1 cut(s)	751
MhlI	GDGCHC	4 cut(s)	742, 1842, 2152, 2467
MlyI	GAGTC	3 cut(s)	482, 1085, 1424
Mph1103I	ATGCAT	2 cut(s)	9, 1850
MroXI	GAANNNNTTC	1 cut(s)	1335
MseI	TTAA	7 cut(s)	435, 545, 1236, 1635, 1656, 1805, 2886
MslI	CAYNNNNRTG	1 cut(s)	2359
MspA1I	CMGCKG	1 cut(s)	2102
MspCI	CTTAAG	1 cut(s)	1634
MspI	CCGG	1 cut(s)	2207
MspR9I	CCNGG	6 cut(s)	201, 705, 2115, 2271, 2727, 2822
MvaI	CCWGG	6 cut(s)	201, 705, 2115, 2271, 2727, 2822
MwoI	GCNNNNNNNGC	3 cut(s)	980, 1376, 2312
NheI	GCTAGC	1 cut(s)	2408
NlaIV	GGNNCC	2 cut(s)	2575, 2998
NmeAIII	GCCGAG	1 cut(s)	1099
NmuCI	GTSAC	3 cut(s)	474, 809, 2858
NsiI	ATGCAT	2 cut(s)	9, 1850
NspI	RCATGY	6 cut(s)	11, 227, 968, 1148, 1319, 1400
NspV	TTCGAA	1 cut(s)	1747
PaeI	GCATGC	2 cut(s)	11, 968
PagI	TCATGA	1 cut(s)	580
PceI	AGGCCT	3 cut(s)	1165, 1379, 2881
PciI	ACATGT	1 cut(s)	1144
PdmI	GAANNNNTTC	1 cut(s)	1335
PfeI	GAWTC	9 cut(s)	173, 821, 827, 863, 935, 1052, 1556, 2559, 2716
PfoI	TCCNGGA	2 cut(s)	2269, 2820
PleI	GAGTC	3 cut(s)	481, 1084, 1424
PmaCI	CACGTG	1 cut(s)	1985
PmlI	CACGTG	1 cut(s)	1985
PpsI	GAGTC	3 cut(s)	481, 1084, 1424
Ppu21I	YACGTR	3 cut(s)	263, 1957, 1985
PpuMI	RGGWCCY	1 cut(s)	1520
PscI	ACATGT	1 cut(s)	1144
Psp124BI	GAGCTC	1 cut(s)	1842
Psp5II	RGGWCCY	1 cut(s)	1520
Psp6I	CCWGG	6 cut(s)	199, 703, 2113, 2269, 2725, 2820
PspCI	CACGTG	1 cut(s)	1985
PspEI	GGTNACC	2 cut(s)	2619, 2635
PspFI	CCCAGC	2 cut(s)	1914, 2315
PspGI	CCWGG	6 cut(s)	199, 703, 2113, 2269, 2725, 2820
PspN4I	GGNNCC	2 cut(s)	2575, 2998
PspPI	GGNCC	6 cut(s)	1133, 1520, 2264, 2541, 2574, 2924
PspPPI	RGGWCCY	1 cut(s)	1520
PstNI	CAGNNNCTG	5 cut(s)	710, 1070, 1253, 1523, 2315
PsuI	RGATCY	1 cut(s)	751
PvuII	CAGCTG	1 cut(s)	2102
RsaI	GTAC	4 cut(s)	45, 118, 789, 2109
RsaNI	GTAC	4 cut(s)	44, 117, 788, 2108
RseI	CAYNNNNRTG	1 cut(s)	2359
SacI	GAGCTC	1 cut(s)	1842
SaqAI	TTAA	7 cut(s)	435, 545, 1236, 1635, 1656, 1805, 2886
Sau96I	GGNCC	6 cut(s)	1133, 1520, 2264, 2541, 2574, 2924
SchI	GAGTC	3 cut(s)	482, 1085, 1424
ScrFI	CCNGG	6 cut(s)	201, 705, 2115, 2271, 2727, 2822
SduI	GDGCHC	4 cut(s)	742, 1842, 2152, 2467
SexAI	ACCWGGT	1 cut(s)	2725
SfaNI	GCATC	7 cut(s)	319, 382, 1267, 1984, 2362, 2593, 2932
SfcI	CTRYAG	3 cut(s)	268, 2103, 2868
SfuI	TTCGAA	1 cut(s)	1747
SinI	GGWCC	2 cut(s)	1520, 2541
SmiI	ATTTAAAT	1 cut(s)	1806
SmiMI	CAYNNNNRTG	1 cut(s)	2359
SmlI	CTYRAG	5 cut(s)	357, 986, 1634, 1826, 2709
SmoI	CTYRAG	5 cut(s)	357, 986, 1634, 1826, 2709
SpeI	ACTAGT	1 cut(s)	911
SphI	GCATGC	2 cut(s)	11, 968
SseBI	AGGCCT	3 cut(s)	1165, 1379, 2881
SsiI	CCGC	5 cut(s)	1100, 1346, 1539, 1724, 2236
SspI	AATATT	4 cut(s)	62, 1752, 2253, 2433
SspMI	CTAG	9 cut(s)	234, 776, 912, 939, 1449, 1862, 2409, 2496, 2720
SstI	GAGCTC	1 cut(s)	1842
StuI	AGGCCT	3 cut(s)	1165, 1379, 2881
StyD4I	CCNGG	6 cut(s)	199, 703, 2113, 2269, 2725, 2820
StyI	CCWWGG	2 cut(s)	2295, 2916
SwaI	ATTTAAAT	1 cut(s)	1806
TaaI	ACNGT	9 cut(s)	85, 121, 137, 272, 550, 818, 2552, 2641, 2872
TaiI	ACGT	7 cut(s)	185, 265, 1186, 1959, 1987, 2163, 2658
TaqI	TCGA	3 cut(s)	1117, 1213, 1747
TatI	WGTACW	2 cut(s)	116, 787
TauI	GCSGC	1 cut(s)	2239
TfiI	GAWTC	9 cut(s)	173, 821, 827, 863, 935, 1052, 1556, 2559, 2716
Tru1I	TTAA	7 cut(s)	435, 545, 1236, 1635, 1656, 1805, 2886
Tru9I	TTAA	7 cut(s)	435, 545, 1236, 1635, 1656, 1805, 2886
TscAI	CASTG	9 cut(s)	88, 140, 220, 324, 715, 790, 1377, 2782, 2877
TseFI	GTSAC	3 cut(s)	474, 809, 2858
TseI	GCWGC	9 cut(s)	210, 614, 887, 980, 1621, 2522, 2801, 2804, 2864
Tsp45I	GTSAC	3 cut(s)	474, 809, 2858
TspGWI	ACGGA	1 cut(s)	522
TspRI	CASTG	9 cut(s)	88, 140, 220, 324, 715, 790, 1377, 2782, 2877
Vha464I	CTTAAG	1 cut(s)	1634
VpaK11BI	GGWCC	2 cut(s)	1520, 2541
XagI	CCTNNNNNAGG	1 cut(s)	2244
XapI	RAATTY	4 cut(s)	385, 1580, 1600, 2053
XceI	RCATGY	6 cut(s)	11, 227, 968, 1148, 1319, 1400
XcmI	CCANNNNNNNNNTGG	4 cut(s)	86, 140, 598, 1305
XmnI	GAANNNNTTC	1 cut(s)	1335
XspI	CTAG	9 cut(s)	234, 776, 912, 939, 1449, 1862, 2409, 2496, 2720
Zsp2I	ATGCAT	2 cut(s)	9, 1850

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.