AT1G50170

CbiX

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003862
Physical Location & Seq
Forward (+)
18581998 .. 18583832
1835 bp
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UTR
Exon/CDS
Intron
AT1G50170.2

Sequence Viewer

Length: 678 bp
ATGACGACTCAGTCGCAGTTCTTAGTGAACCTCTCTTACGGAGGCCTCGCCTCCCAAAGTAATCTCAGAGCGAATAATCGTGTTTCACCTTCTTCTTGTCAAATTACACGAACAAATCGCTCATGGGCATTACCAGTTTCCTTTAAAGTTGAGAAATTTCAACTCCAACGAGGAAGGAGGAGAAGAGGATCACCATGTTTTGGGGAGAGCGAAGGGTTGGTCAAAAATGGGATTGGTGATGCTGATGGGATTATAATTGTTGATCACGGTTCTCGTCGCCGTGAATCCAATCTTATGCTTGAGGAGTTTGTGAAAATGTTCAAAGAAAAAACTGGTTACCCAATTGTAGAGCCTGCTCATATGGAATTGGCTGAGCCATCTATAAAAGATGCATTCAGTTTGTGTGTACAACAAGGGGCGAAACGTGTAGTTGTGAGCCCGTTTTTTCTATTTCCTGGAAGACATTGGCATACGGACATTCCCTCGTTGACGGCTGATGCTGCAAAGGAGTTCTCTGGCATCTCATACCTCATAACTGCACCTCTCGGCCCCCATAATCTCCTCCTGGACGTTGTAAATGACAGAATCCAACATTGCTTGAGCCATGTTGAAGGTGATGCTGACGAGTGTCTTGTTTGTGCTGGAACAAATAAGTGCAAGCTCTACAATAGTTCTTAG

Protein Analysis

225

Amino Acids

24.93

Weight (kDa)

8.48

Isoelectric Point (pI)

52.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CbiX PF01903 89 - 189 8.5e-31 CbiX
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
AasI GACNNNNNNGTC 1 cut(s) 10
AccB7I CCANNNNNTGG 1 cut(s) 200
AclWI GGATC 1 cut(s) 196
AcsI RAATTY 1 cut(s) 155
AfaI GTAC 1 cut(s) 408
AfiI CCNNNNNNNGG 1 cut(s) 200
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 3 cut(s) 161, 322, 611
AjnI CCWGG 2 cut(s) 454, 564
AluBI AGCT 1 cut(s) 661
AluI AGCT 1 cut(s) 661
AlwI GGATC 1 cut(s) 196
AoxI GGCC 2 cut(s) 43, 547
ApeKI GCWGC 1 cut(s) 500
ApoI RAATTY 1 cut(s) 155
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
Asp700I GAANNNNTTC 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 548
AsuHPI GGTGA 4 cut(s) 78, 183, 248, 626
BanII GRGCYC 1 cut(s) 440
BbsI GAAGAC 1 cut(s) 466
BbvI GCAGC 1 cut(s) 487
BccI CCATC 2 cut(s) 239, 385
BceAI ACGGC 2 cut(s) 264, 507
BciT130I CCWGG 2 cut(s) 456, 566
BclI TGATCA 1 cut(s) 262
BisI GCNGC 1 cut(s) 501
BlpI GCTNAGC 1 cut(s) 372
BlsI GCNGC 1 cut(s) 502
Bme1390I CCNGG 2 cut(s) 456, 566
BmgT120I GGNCC 1 cut(s) 548
BmiI GGNNCC 1 cut(s) 550
BmrFI CCNGG 2 cut(s) 456, 566
BmsI GCATC 5 cut(s) 229, 379, 487, 528, 607
BoxI GACNNNNGTC 1 cut(s) 627
BpiI GAAGAC 1 cut(s) 466
Bpu1102I GCTNAGC 1 cut(s) 372
BpuEI CTTGAG 2 cut(s) 320, 619
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse1I ACTGG 2 cut(s) 134, 337
Bse3DI GCAATG 1 cut(s) 592
BseBI CCWGG 2 cut(s) 456, 566
BseLI CCNNNNNNNGG 1 cut(s) 200
BseMI GCAATG 1 cut(s) 592
BseMII CTCAG 3 cut(s) 23, 79, 363
BseNI ACTGG 2 cut(s) 134, 337
BseRI GAGGAG 3 cut(s) 193, 317, 551
BseXI GCAGC 1 cut(s) 487
BsgI GTGCAG 1 cut(s) 522
BshFI GGCC 2 cut(s) 45, 549
BslI CCNNNNNNNGG 1 cut(s) 200
BsmI GAATGC 1 cut(s) 392
BsnI GGCC 2 cut(s) 45, 549
Bsp1286I GDGCHC 1 cut(s) 440
Bsp1407I TGTACA 1 cut(s) 406
Bsp143I GATC 2 cut(s) 188, 262
Bsp1720I GCTNAGC 1 cut(s) 372
BspANI GGCC 2 cut(s) 45, 549
BspCNI CTCAG 3 cut(s) 22, 78, 364
BspLI GGNNCC 1 cut(s) 550
BspPI GGATC 1 cut(s) 196
BsrDI GCAATG 1 cut(s) 592
BsrGI TGTACA 1 cut(s) 406
BsrI ACTGG 2 cut(s) 134, 337
BssMI GATC 2 cut(s) 188, 262
Bst2UI CCWGG 2 cut(s) 456, 566
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 1 cut(s) 178
BstAUI TGTACA 1 cut(s) 406
BstC8I GCNNGC 2 cut(s) 354, 659
BstDEI CTNAG 5 cut(s) 9, 22, 65, 372, 675
BstEII GGTNACC 1 cut(s) 335
BstKTI GATC 2 cut(s) 191, 265
BstMBI GATC 2 cut(s) 188, 262
BstMWI GCNNNNNNNGC 1 cut(s) 500
BstNI CCWGG 2 cut(s) 456, 566
BstPAI GACNNNNGTC 1 cut(s) 627
BstPI GGTNACC 1 cut(s) 335
BstSCI CCNGG 2 cut(s) 454, 564
BstV1I GCAGC 1 cut(s) 487
BstV2I GAAGAC 1 cut(s) 466
BsuRI GGCC 2 cut(s) 45, 549
Cac8I GCNNGC 2 cut(s) 354, 659
Cfr13I GGNCC 1 cut(s) 548
Csp6I GTAC 1 cut(s) 407
CviAII CATG 3 cut(s) 123, 195, 605
CviJI RGCY 9 cut(s) 45, 352, 371, 376, 438, 494, 549, 603, 661
CviKI_1 RGCY 9 cut(s) 45, 352, 371, 376, 438, 494, 549, 603, 661
CviQI GTAC 1 cut(s) 407
DdeI CTNAG 5 cut(s) 9, 22, 65, 372, 675
DpnI GATC 2 cut(s) 190, 264
DpnII GATC 2 cut(s) 188, 262
DraI TTTAAA 1 cut(s) 145
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
Eco147I AGGCCT 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 440
Eco91I GGTNACC 1 cut(s) 335
EcoO65I GGTNACC 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 454, 564
EcoT22I ATGCAT 1 cut(s) 394
EcoT38I GRGCYC 1 cut(s) 440
FaeI CATG 3 cut(s) 126, 198, 608
FatI CATG 3 cut(s) 122, 194, 604
FauNDI CATATG 1 cut(s) 360
FbaI TGATCA 1 cut(s) 262
Fnu4HI GCNGC 1 cut(s) 501
FriOI GRGCYC 1 cut(s) 440
Fsp4HI GCNGC 1 cut(s) 501
GluI GCNGC 1 cut(s) 501
HaeIII GGCC 2 cut(s) 45, 549
Hin1II CATG 3 cut(s) 126, 198, 608
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HinfI GANTC 3 cut(s) 7, 284, 585
HphI GGTGA 4 cut(s) 78, 183, 248, 626
Hpy166II GTNNAC 3 cut(s) 28, 407, 489
Hpy188I TCNGA 1 cut(s) 68
Hpy8I GTNNAC 3 cut(s) 28, 407, 489
Hpy99I CGWCG 1 cut(s) 279
HpyAV CCTTC 4 cut(s) 99, 168, 206, 605
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 2 cut(s) 424, 570
HpyCH4V TGCA 4 cut(s) 392, 503, 539, 657
HpyF10VI GCNNNNNNNGC 1 cut(s) 500
HpyF3I CTNAG 5 cut(s) 9, 22, 65, 372, 675
HpySE526I ACGT 2 cut(s) 424, 570
Hsp92II CATG 3 cut(s) 126, 198, 608
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 2 cut(s) 188, 262
LpnPI CCDG 9 cut(s) 147, 318, 366, 441, 468, 501, 551, 578, 627
Lsp1109I GCAGC 1 cut(s) 487
LweI GCATC 5 cut(s) 229, 379, 487, 528, 607
MaeII ACGT 2 cut(s) 424, 570
MaeIII GTNAC 1 cut(s) 335
MalI GATC 2 cut(s) 190, 264
MboI GATC 2 cut(s) 188, 262
MboII GAAGA 3 cut(s) 84, 195, 471
MfeI CAATTG 1 cut(s) 342
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 5 cut(s) 102, 155, 255, 342, 365
MmeI TCCRAC 2 cut(s) 190, 613
Mph1103I ATGCAT 1 cut(s) 394
MroXI GAANNNNTTC 1 cut(s) 317
MseI TTAA 1 cut(s) 144
MspR9I CCNGG 2 cut(s) 456, 566
MunI CAATTG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 392
MvaI CCWGG 2 cut(s) 456, 566
MwoI GCNNNNNNNGC 1 cut(s) 500
NdeI CATATG 1 cut(s) 360
NdeII GATC 2 cut(s) 188, 262
NlaIII CATG 3 cut(s) 126, 198, 608
NlaIV GGNNCC 1 cut(s) 550
NmeAIII GCCGAG 1 cut(s) 525
NsiI ATGCAT 1 cut(s) 394
PceI AGGCCT 1 cut(s) 45
PctI GAATGC 1 cut(s) 392
PdmI GAANNNNTTC 1 cut(s) 317
PfeI GAWTC 2 cut(s) 284, 585
PflFI GACNNNGTC 1 cut(s) 10
PflMI CCANNNNNTGG 1 cut(s) 200
PfoI TCCNGGA 2 cut(s) 454, 564
PkrI GCNGC 1 cut(s) 502
PshAI GACNNNNGTC 1 cut(s) 627
PsiI TTATAA 1 cut(s) 254
Psp6I CCWGG 2 cut(s) 454, 564
PspEI GGTNACC 1 cut(s) 335
PspGI CCWGG 2 cut(s) 454, 564
PspN4I GGNNCC 1 cut(s) 550
PspPI GGNCC 1 cut(s) 548
PsrI GAACNNNNNNTAC 2 cut(s) 20, 52
PsyI GACNNNGTC 1 cut(s) 10
RsaI GTAC 1 cut(s) 408
RsaNI GTAC 1 cut(s) 407
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 2 cut(s) 188, 262
Sau96I GGNCC 1 cut(s) 548
ScrFI CCNGG 2 cut(s) 456, 566
SduI GDGCHC 1 cut(s) 440
SetI ASST 8 cut(s) 33, 91, 427, 531, 544, 573, 616, 663
SfaNI GCATC 5 cut(s) 229, 379, 487, 528, 607
SmlI CTYRAG 2 cut(s) 299, 598
SmoI CTYRAG 2 cut(s) 299, 598
Sse9I AATT 5 cut(s) 102, 155, 255, 342, 365
SseBI AGGCCT 1 cut(s) 45
StuI AGGCCT 1 cut(s) 45
StyD4I CCNGG 2 cut(s) 454, 564
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 2 cut(s) 427, 573
TasI AATT 5 cut(s) 102, 155, 255, 342, 365
TatI WGTACW 1 cut(s) 406
TfiI GAWTC 2 cut(s) 284, 585
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseI GCWGC 1 cut(s) 500
TspGWI ACGGA 2 cut(s) 54, 488
Tth111I GACNNNGTC 1 cut(s) 10
Van91I CCANNNNNTGG 1 cut(s) 200
XapI RAATTY 1 cut(s) 155
XmnI GAANNNNTTC 1 cut(s) 317
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.