Rorug02G0565000

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002928
Physical Location & Seq
Reverse (-)
69014904 .. 69016813
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0565000.1

Sequence Viewer

Length: 663 bp
ATGGCTGCAAATCATCCCTTCCGAGTTCCGAAAAAGCCTTGCACAGTCGACATCGTTTCGGATCCGGTGTGCCCTTGGTGCTTTATAGGCAAGAAAAATCTTGACAAAGCTCTAGAGGAGGCTGACCCTCTGTACGTGTTTGAGCTCCGGTGGCATCCATTTCAAACTTATCCTGATATCCCTAAAGAAGGCATTGATAAGAAAAAGCATTCTGAGGATAAGCATGGCAGCACTCATATATCAGAAGTTGTGGAAATGCGTATAGGAGATATTTTGAGAACCACCTACGATGCGCTTGAATACAAACTTTCTGGAATCATGGGAGATCCTCTAGATTATCACAGGCTTGTATATTTTGCTGGGGAACAGGATCATGATATGCAACATGATCTTGTGGATGAGATACTGCTTGGGTACTTCACAGAGGAAAAGGACATTGCAGACAGGGAATATCTTGTGGAATGTGCTGGTAAGATTGGCATAGAAGGGGCAGCAGAGTTTCTTGAAGACCCTAACAATGGGCTCAATGAGGTCACTGAAGATCTTAAGAAGTACTCGGGAACAAAACAAGTCCCATATTACGTGTTTAATGGTAAGGTGGACTGCGTCGGTGCTCAGCCGTCTGAGGTGTTCATGAGAGCTTTCGAAGCAGCCACAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

220

Amino Acids

25.05

Weight (kDa)

4.86

Isoelectric Point (pI)

31.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSBA PF01323 15 - 214 2.6e-21 DSBA-like thioredoxin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 48
AclWI GGATC 4 cut(s) 56, 69, 320, 378
AcuI CTGAAG 1 cut(s) 558
AfaI GTAC 3 cut(s) 134, 416, 554
AfiI CCNNNNNNNGG 2 cut(s) 188, 518
AflII CTTAAG 1 cut(s) 545
AflIII ACRYGT 2 cut(s) 135, 582
AgsI TTSAA 3 cut(s) 164, 299, 506
AluBI AGCT 3 cut(s) 110, 145, 641
AluI AGCT 3 cut(s) 110, 145, 641
Alw21I GWGCWC 2 cut(s) 147, 616
AlwI GGATC 4 cut(s) 56, 69, 320, 378
Ama87I CYCGRG 1 cut(s) 556
ApeKI GCWGC 4 cut(s) 5, 228, 491, 650
AspLEI GCGC 1 cut(s) 295
AsuII TTCGAA 1 cut(s) 645
AvaI CYCGRG 1 cut(s) 556
BaeGI GKGCMC 1 cut(s) 74
BamHI GGATCC 1 cut(s) 61
BanII GRGCYC 2 cut(s) 147, 525
BbsI GAAGAC 1 cut(s) 513
Bbv12I GWGCWC 2 cut(s) 147, 616
BbvI GCAGC 2 cut(s) 240, 503
BceAI ACGGC 1 cut(s) 604
BfaI CTAG 2 cut(s) 113, 332
BfrI CTTAAG 1 cut(s) 545
BglII AGATCT 1 cut(s) 541
BisI GCNGC 4 cut(s) 6, 229, 492, 651
BlpI GCTNAGC 1 cut(s) 615
BlsI GCNGC 4 cut(s) 7, 230, 493, 652
BmcAI AGTACT 1 cut(s) 554
BmeT110I CYCGRG 1 cut(s) 556
BmiI GGNNCC 1 cut(s) 63
BmsI GCATC 2 cut(s) 163, 280
BpiI GAAGAC 1 cut(s) 513
Bpu1102I GCTNAGC 1 cut(s) 615
Bpu14I TTCGAA 1 cut(s) 645
BsaAI YACGTR 2 cut(s) 136, 583
BsaJI CCNNGG 1 cut(s) 74
BsaWI WCCGGW 2 cut(s) 64, 147
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 518
Bse3DI GCAATG 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 74
BseGI GGATG 3 cut(s) 13, 154, 403
BseLI CCNNNNNNNGG 2 cut(s) 188, 518
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 3 cut(s) 204, 615, 629
BseRI GAGGAG 1 cut(s) 131
BseSI GKGCMC 1 cut(s) 74
BseXI GCAGC 2 cut(s) 240, 503
BseYI CCCAGC 1 cut(s) 359
BsiHKAI GWGCWC 2 cut(s) 147, 616
BsiHKCI CYCGRG 1 cut(s) 556
BsiSI CCGG 2 cut(s) 65, 148
BslFI GGGAC 1 cut(s) 557
BslI CCNNNNNNNGG 2 cut(s) 188, 518
BsmFI GGGAC 1 cut(s) 557
BsmI GAATGC 1 cut(s) 208
BsoBI CYCGRG 1 cut(s) 556
Bsp119I TTCGAA 1 cut(s) 645
Bsp1286I GDGCHC 4 cut(s) 74, 147, 525, 616
Bsp143I GATC 5 cut(s) 61, 325, 370, 388, 541
Bsp1720I GCTNAGC 1 cut(s) 615
BspCNI CTCAG 3 cut(s) 205, 616, 628
BspHI TCATGA 2 cut(s) 373, 633
BspLI GGNNCC 1 cut(s) 63
BspPI GGATC 4 cut(s) 56, 69, 320, 378
BspT104I TTCGAA 1 cut(s) 645
BspTI CTTAAG 1 cut(s) 545
BsrDI GCAATG 1 cut(s) 435
BssECI CCNNGG 1 cut(s) 74
BssMI GATC 5 cut(s) 61, 325, 370, 388, 541
BssT1I CCWWGG 1 cut(s) 74
Bst4CI ACNGT 1 cut(s) 46
BstAFI CTTAAG 1 cut(s) 545
BstBAI YACGTR 2 cut(s) 136, 583
BstBI TTCGAA 1 cut(s) 645
BstDEI CTNAG 3 cut(s) 213, 615, 624
BstENI CCTNNNNNAGG 1 cut(s) 186
BstF5I GGATG 3 cut(s) 13, 154, 403
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 5 cut(s) 64, 328, 373, 391, 544
BstMBI GATC 5 cut(s) 61, 325, 370, 388, 541
BstMWI GCNNNNNNNGC 4 cut(s) 78, 87, 151, 647
BstSLI GKGCMC 1 cut(s) 74
BstV1I GCAGC 2 cut(s) 240, 503
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 3 cut(s) 61, 325, 541
BstYI RGATCY 3 cut(s) 61, 325, 541
BtsCI GGATG 3 cut(s) 13, 154, 403
BtsIMutI CAGTG 1 cut(s) 534
CciI TCATGA 2 cut(s) 373, 633
CfoI GCGC 1 cut(s) 295
CseI GACGC 1 cut(s) 595
Csp6I GTAC 3 cut(s) 133, 415, 553
CviAII CATG 5 cut(s) 224, 319, 374, 386, 634
CviQI GTAC 3 cut(s) 133, 415, 553
DdeI CTNAG 3 cut(s) 213, 615, 624
DpnI GATC 5 cut(s) 63, 327, 372, 390, 543
DpnII GATC 5 cut(s) 61, 325, 370, 388, 541
Ecl136II GAGCTC 1 cut(s) 145
Eco130I CCWWGG 1 cut(s) 74
Eco24I GRGCYC 2 cut(s) 147, 525
Eco32I GATATC 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 558
Eco88I CYCGRG 1 cut(s) 556
EcoICRI GAGCTC 1 cut(s) 145
EcoNI CCTNNNNNAGG 1 cut(s) 186
EcoRV GATATC 1 cut(s) 178
EcoT14I CCWWGG 1 cut(s) 74
EcoT38I GRGCYC 2 cut(s) 147, 525
ErhI CCWWGG 1 cut(s) 74
FaeI CATG 5 cut(s) 227, 322, 377, 389, 637
FaqI GGGAC 1 cut(s) 557
FatI CATG 5 cut(s) 223, 318, 373, 385, 633
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 4 cut(s) 6, 229, 492, 651
FokI GGATG 2 cut(s) 141, 410
FriOI GRGCYC 2 cut(s) 147, 525
Fsp4HI GCNGC 4 cut(s) 6, 229, 492, 651
FspBI CTAG 2 cut(s) 113, 332
GlaI GCGC 1 cut(s) 294
GluI GCNGC 4 cut(s) 6, 229, 492, 651
GsaI CCCAGC 1 cut(s) 363
HapII CCGG 2 cut(s) 65, 148
HgaI GACGC 1 cut(s) 595
HhaI GCGC 1 cut(s) 295
Hin1II CATG 5 cut(s) 227, 322, 377, 389, 637
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 1 cut(s) 315
HpaII CCGG 2 cut(s) 65, 148
Hpy166II GTNNAC 2 cut(s) 49, 601
Hpy188I TCNGA 6 cut(s) 23, 30, 61, 214, 244, 625
Hpy188III TCNNGA 9 cut(s) 101, 113, 173, 312, 332, 374, 503, 558, 634
Hpy8I GTNNAC 2 cut(s) 49, 601
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 3 cut(s) 28, 182, 479
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 2 cut(s) 135, 582
HpyCH4V TGCA 4 cut(s) 8, 42, 382, 440
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 87, 151, 647
HpyF3I CTNAG 3 cut(s) 213, 615, 624
HpySE526I ACGT 2 cut(s) 135, 582
Hsp92II CATG 5 cut(s) 227, 322, 377, 389, 637
HspAI GCGC 1 cut(s) 293
Kzo9I GATC 5 cut(s) 61, 325, 370, 388, 541
LmnI GCTCC 1 cut(s) 150
LpnPI CCDG 9 cut(s) 78, 161, 186, 297, 328, 345, 353, 430, 453
Lsp1109I GCAGC 2 cut(s) 240, 503
LweI GCATC 2 cut(s) 163, 280
MaeI CTAG 2 cut(s) 113, 332
MaeII ACGT 2 cut(s) 135, 582
MaeIII GTNAC 1 cut(s) 532
MalI GATC 5 cut(s) 63, 327, 372, 390, 543
MboI GATC 5 cut(s) 61, 325, 370, 388, 541
MboII GAAGA 2 cut(s) 518, 551
MflI RGATCY 3 cut(s) 61, 325, 541
MhlI GDGCHC 4 cut(s) 74, 147, 525, 616
MnlI CCTC 8 cut(s) 109, 112, 138, 208, 339, 418, 523, 619
MseI TTAA 2 cut(s) 546, 588
MspCI CTTAAG 1 cut(s) 545
MspI CCGG 2 cut(s) 65, 148
Mva1269I GAATGC 1 cut(s) 208
MwoI GCNNNNNNNGC 4 cut(s) 78, 87, 151, 647
NdeII GATC 5 cut(s) 61, 325, 370, 388, 541
NlaIII CATG 5 cut(s) 227, 322, 377, 389, 637
NlaIV GGNNCC 1 cut(s) 63
NmuCI GTSAC 1 cut(s) 532
NspV TTCGAA 1 cut(s) 645
PagI TCATGA 2 cut(s) 373, 633
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 315
PflFI GACNNNGTC 1 cut(s) 605
PkrI GCNGC 4 cut(s) 7, 230, 493, 652
Ppu21I YACGTR 2 cut(s) 136, 583
Psp124BI GAGCTC 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 359
PspN4I GGNNCC 1 cut(s) 63
PsuI RGATCY 3 cut(s) 61, 325, 541
PsyI GACNNNGTC 1 cut(s) 605
RsaI GTAC 3 cut(s) 134, 416, 554
RsaNI GTAC 3 cut(s) 133, 415, 553
SacI GAGCTC 1 cut(s) 147
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 2 cut(s) 546, 588
SatI GCNGC 4 cut(s) 6, 229, 492, 651
Sau3AI GATC 5 cut(s) 61, 325, 370, 388, 541
ScaI AGTACT 1 cut(s) 554
SduI GDGCHC 4 cut(s) 74, 147, 525, 616
SetI ASST 9 cut(s) 112, 138, 147, 287, 534, 585, 600, 630, 643
SfaNI GCATC 2 cut(s) 163, 280
SfuI TTCGAA 1 cut(s) 645
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
SspMI CTAG 2 cut(s) 113, 332
SstI GAGCTC 1 cut(s) 147
StyI CCWWGG 1 cut(s) 74
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 2 cut(s) 138, 585
TaqI TCGA 2 cut(s) 48, 645
TatI WGTACW 1 cut(s) 552
TfiI GAWTC 1 cut(s) 315
Tru1I TTAA 2 cut(s) 546, 588
Tru9I TTAA 2 cut(s) 546, 588
TscAI CASTG 1 cut(s) 541
TseFI GTSAC 1 cut(s) 532
TseI GCWGC 4 cut(s) 5, 228, 491, 650
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 1 cut(s) 622
TspRI CASTG 1 cut(s) 541
Tth111I GACNNNGTC 1 cut(s) 605
Vha464I CTTAAG 1 cut(s) 545
XagI CCTNNNNNAGG 1 cut(s) 186
XbaI TCTAGA 2 cut(s) 112, 331
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 2 cut(s) 113, 332
ZrmI AGTACT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.