Rh3AG299000

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002928
Physical Location & Seq
Reverse (-)
36615084 .. 36631128
16045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG299000.1

Sequence Viewer

Length: 912 bp
ATGGCGGCAGCGGTGATTTATGGATATATGGTGGAGGTGGTGAAGGTGATGGGGGTGGTGAAGACTTGTAGACATATGGAGGAGGAGGTAAAAGTGAGGGTGGTGGCGGTGATTTATACGGTGGCAGCAGCAATGGTGATAGAGGAGGAGGGGATTTGTAATGATATGGAGGATGTATCACATGCTTTGGTGGAGGTGGGGGAGGAGGTGACTTGTAATGGTGTCGAGGGTGCTTGGCATATTTTGGTGGTGGTGATTGATACTTGGGAGGAGGGTATCGAGTGGCATGGTGAGGACGTGGTGGTGAAGAATAAGTGTAATGATTGTGAAGATTGGCTTGATGATTTTGTCTGCTGCTGCATCCCTCTTTACAATCCAAAAATGGATCCTGTTATTCCTGTACAAAACTGGAGAAAAGGATCTCAGTGGGTTGTATTGACGAGGAAGCATGCAGAGGTTGTAGTGAAAGACGATACAGTGTTTCCTATGTTTCAGTTGTATTGTAAGAGGAAGTCGTTACCTGAGTTTTGGCGGGATCATCCTGTTCCGGCCGATAACTCCAAGGAGCACAATTGCATTCCAGACGAGCATTATGTTCAGACAATACTGGCTCAAGAAGGCCTTGAAGGAGAAATCACACGAAGATCACTGACACACTCATCATGGGATTTATCATCCACTGCAGACCGTGAACGCCGTGGATGGCACCCTATGACATACAAATATTCAGATGCTACTCCCATGCTTATTAAATCTATAAAGGATGTAGATAATATCTACTATGAGACCGAGTATCGAAGAGAATGGTGTAGCAGCAAGGAGAAACCCTCTCCATGCTTTCTTTTTGCCAGAAAATTTACCCGGCCTGCTGCTCTCAGGCTTCTCAACACGACTACACTGGAGCTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

303

Amino Acids

35.02

Weight (kDa)

5.23

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 124 - 253 1.3e-28 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 705
AccI GTMKAC 1 cut(s) 70
AciI CCGC 4 cut(s) 5, 11, 107, 532
AclWI GGATC 4 cut(s) 380, 393, 427, 543
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 1 cut(s) 854
AfaI GTAC 1 cut(s) 402
AgsI TTSAA 1 cut(s) 626
AjiI CACGTC 1 cut(s) 298
AluBI AGCT 1 cut(s) 904
AluI AGCT 1 cut(s) 904
Alw21I GWGCWC 1 cut(s) 570
Alw26I GTCTC 1 cut(s) 779
AlwI GGATC 4 cut(s) 380, 393, 427, 543
AoxI GGCC 3 cut(s) 549, 619, 863
ApeKI GCWGC 7 cut(s) 8, 125, 128, 354, 357, 813, 869
ApoI RAATTY 1 cut(s) 854
AsuC2I CCSGG 1 cut(s) 862
BamHI GGATCC 1 cut(s) 385
BanI GGYRCC 1 cut(s) 705
BbsI GAAGAC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 570
BbvI GCAGC 7 cut(s) 20, 137, 140, 341, 344, 825, 856
BccI CCATC 2 cut(s) 43, 696
BceAI ACGGC 1 cut(s) 681
BcnI CCSGG 1 cut(s) 862
BcoDI GTCTC 1 cut(s) 779
BfmI CTRYAG 1 cut(s) 681
BisI GCNGC 8 cut(s) 6, 9, 126, 129, 355, 358, 814, 870
BlsI GCNGC 8 cut(s) 7, 10, 127, 130, 356, 359, 815, 871
Bme1390I CCNGG 1 cut(s) 862
BmgBI CACGTC 1 cut(s) 298
BmiI GGNNCC 2 cut(s) 387, 707
BmrFI CCNGG 1 cut(s) 862
BmsI GCATC 2 cut(s) 369, 721
BpiI GAAGAC 1 cut(s) 68
BplI GAGNNNNNCTC 2 cut(s) 812, 844
BpmI CTGGAG 1 cut(s) 430
BpuEI CTTGAG 1 cut(s) 597
BpuMI CCSGG 1 cut(s) 862
BsaI GGTCTC 1 cut(s) 779
BsaJI CCNNGG 2 cut(s) 561, 697
Bse1I ACTGG 3 cut(s) 413, 612, 903
Bse3DI GCAATG 1 cut(s) 138
BseDI CCNNGG 2 cut(s) 561, 697
BseGI GGATG 6 cut(s) 178, 360, 538, 674, 707, 769
BseMI GCAATG 1 cut(s) 138
BseMII CTCAG 3 cut(s) 437, 513, 889
BseNI ACTGG 3 cut(s) 413, 612, 903
BseRI GAGGAG 6 cut(s) 95, 98, 158, 161, 218, 284
BseX3I CGGCCG 1 cut(s) 549
BseXI GCAGC 7 cut(s) 20, 137, 140, 341, 344, 825, 856
Bsh1285I CGRYCG 1 cut(s) 552
BshFI GGCC 3 cut(s) 551, 621, 865
BshNI GGYRCC 1 cut(s) 705
BsiEI CGRYCG 1 cut(s) 552
BsiHKAI GWGCWC 1 cut(s) 570
BsiSI CCGG 2 cut(s) 548, 862
BsmAI GTCTC 1 cut(s) 779
BsmI GAATGC 1 cut(s) 576
BsnI GGCC 3 cut(s) 551, 621, 865
Bso31I GGTCTC 1 cut(s) 779
Bsp1286I GDGCHC 1 cut(s) 570
Bsp1407I TGTACA 1 cut(s) 400
Bsp143I GATC 4 cut(s) 385, 419, 535, 644
BspACI CCGC 4 cut(s) 5, 11, 107, 532
BspANI GGCC 3 cut(s) 551, 621, 865
BspCNI CTCAG 3 cut(s) 436, 514, 888
BspLI GGNNCC 2 cut(s) 387, 707
BspMAI CTGCAG 1 cut(s) 685
BspPI GGATC 4 cut(s) 380, 393, 427, 543
BspT107I GGYRCC 1 cut(s) 705
BspTNI GGTCTC 1 cut(s) 779
BsrDI GCAATG 1 cut(s) 138
BsrGI TGTACA 1 cut(s) 400
BsrI ACTGG 3 cut(s) 413, 612, 903
BssECI CCNNGG 2 cut(s) 561, 697
BssMI GATC 4 cut(s) 385, 419, 535, 644
BssT1I CCWWGG 1 cut(s) 561
Bst4CI ACNGT 3 cut(s) 121, 478, 689
Bst6I CTCTTC 1 cut(s) 793
BstAUI TGTACA 1 cut(s) 400
BstC8I GCNNGC 2 cut(s) 450, 867
BstDEI CTNAG 4 cut(s) 423, 522, 875, 905
BstDSI CCRYGG 1 cut(s) 697
BstF5I GGATG 6 cut(s) 178, 360, 538, 674, 707, 769
BstKTI GATC 4 cut(s) 388, 422, 538, 647
BstMAI GTCTC 1 cut(s) 779
BstMBI GATC 4 cut(s) 385, 419, 535, 644
BstMCI CGRYCG 1 cut(s) 552
BstNSI RCATGY 2 cut(s) 185, 452
BstSCI CCNGG 1 cut(s) 860
BstSFI CTRYAG 1 cut(s) 681
BstV1I GCAGC 7 cut(s) 20, 137, 140, 341, 344, 825, 856
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 2 cut(s) 385, 419
BstYI RGATCY 2 cut(s) 385, 419
BstZI CGGCCG 1 cut(s) 549
BsuRI GGCC 3 cut(s) 551, 621, 865
BtgI CCRYGG 1 cut(s) 697
BtrI CACGTC 1 cut(s) 298
BtsCI GGATG 6 cut(s) 178, 360, 538, 674, 707, 769
BtsI GCAGTG 1 cut(s) 678
BtsIMutI CAGTG 5 cut(s) 431, 483, 647, 678, 896
Cac8I GCNNGC 2 cut(s) 450, 867
Csp6I GTAC 1 cut(s) 401
CviAII CATG 6 cut(s) 182, 287, 449, 663, 742, 834
CviJI RGCY 7 cut(s) 337, 551, 611, 621, 865, 880, 904
CviKI_1 RGCY 7 cut(s) 337, 551, 611, 621, 865, 880, 904
CviQI GTAC 1 cut(s) 401
DdeI CTNAG 4 cut(s) 423, 522, 875, 905
DpnI GATC 4 cut(s) 387, 421, 537, 646
DpnII GATC 4 cut(s) 385, 419, 535, 644
EaeI YGGCCR 1 cut(s) 549
EagI CGGCCG 1 cut(s) 549
Eam1104I CTCTTC 1 cut(s) 793
EarI CTCTTC 1 cut(s) 793
EclXI CGGCCG 1 cut(s) 549
Eco130I CCWWGG 1 cut(s) 561
Eco147I AGGCCT 1 cut(s) 621
Eco31I GGTCTC 1 cut(s) 779
Eco52I CGGCCG 1 cut(s) 549
EcoT14I CCWWGG 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 6 cut(s) 185, 290, 452, 666, 745, 837
FalI AAGNNNNNCTT 4 cut(s) 321, 353, 606, 638
FatI CATG 6 cut(s) 181, 286, 448, 662, 741, 833
FauI CCCGC 1 cut(s) 525
FauNDI CATATG 1 cut(s) 75
FblI GTMKAC 1 cut(s) 70
Fnu4HI GCNGC 8 cut(s) 6, 9, 126, 129, 355, 358, 814, 870
FokI GGATG 6 cut(s) 185, 347, 525, 661, 714, 776
Fsp4HI GCNGC 8 cut(s) 6, 9, 126, 129, 355, 358, 814, 870
GluI GCNGC 8 cut(s) 6, 9, 126, 129, 355, 358, 814, 870
GsuI CTGGAG 1 cut(s) 430
HaeIII GGCC 3 cut(s) 551, 621, 865
HapII CCGG 2 cut(s) 548, 862
Hin1II CATG 6 cut(s) 185, 290, 452, 666, 745, 837
HpaII CCGG 2 cut(s) 548, 862
Hpy166II GTNNAC 2 cut(s) 71, 692
Hpy188I TCNGA 2 cut(s) 600, 730
Hpy188III TCNNGA 2 cut(s) 581, 614
Hpy8I GTNNAC 2 cut(s) 71, 692
HpyAV CCTTC 3 cut(s) 37, 611, 620
HpyCH4III ACNGT 3 cut(s) 121, 478, 689
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 4 cut(s) 360, 452, 576, 683
HpyF3I CTNAG 4 cut(s) 423, 522, 875, 905
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 6 cut(s) 185, 290, 452, 666, 745, 837
Kzo9I GATC 4 cut(s) 385, 419, 535, 644
LmnI GCTCC 2 cut(s) 565, 901
Lsp1109I GCAGC 7 cut(s) 20, 137, 140, 341, 344, 825, 856
LweI GCATC 2 cut(s) 369, 721
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 2 cut(s) 208, 516
MalI GATC 4 cut(s) 387, 421, 537, 646
MboI GATC 4 cut(s) 385, 419, 535, 644
MboII GAAGA 5 cut(s) 73, 319, 341, 654, 810
MfeI CAATTG 1 cut(s) 571
MflI RGATCY 2 cut(s) 385, 419
MhlI GDGCHC 1 cut(s) 570
MluCI AATT 2 cut(s) 571, 854
MseI TTAA 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 2 cut(s) 548, 862
MspR9I CCNGG 1 cut(s) 862
MunI CAATTG 1 cut(s) 571
Mva1269I GAATGC 1 cut(s) 576
NciI CCSGG 1 cut(s) 862
NdeI CATATG 1 cut(s) 75
NdeII GATC 4 cut(s) 385, 419, 535, 644
NlaIII CATG 6 cut(s) 185, 290, 452, 666, 745, 837
NlaIV GGNNCC 2 cut(s) 387, 707
NmuCI GTSAC 1 cut(s) 208
NspI RCATGY 2 cut(s) 185, 452
PaeI GCATGC 1 cut(s) 452
PceI AGGCCT 1 cut(s) 621
PctI GAATGC 1 cut(s) 576
PkrI GCNGC 8 cut(s) 7, 10, 127, 130, 356, 359, 815, 871
PspN4I GGNNCC 2 cut(s) 387, 707
PstI CTGCAG 1 cut(s) 685
PsuI RGATCY 2 cut(s) 385, 419
RsaI GTAC 1 cut(s) 402
RsaNI GTAC 1 cut(s) 401
SaqAI TTAA 1 cut(s) 750
SatI GCNGC 8 cut(s) 6, 9, 126, 129, 355, 358, 814, 870
Sau3AI GATC 4 cut(s) 385, 419, 535, 644
ScrFI CCNGG 1 cut(s) 862
SduI GDGCHC 1 cut(s) 570
SetI ASST 9 cut(s) 39, 48, 90, 198, 210, 300, 459, 523, 906
SfaNI GCATC 2 cut(s) 369, 721
SfcI CTRYAG 1 cut(s) 681
SmlI CTYRAG 1 cut(s) 612
SmoI CTYRAG 1 cut(s) 612
SphI GCATGC 1 cut(s) 452
Sse9I AATT 2 cut(s) 571, 854
SseBI AGGCCT 1 cut(s) 621
SsiI CCGC 4 cut(s) 5, 11, 107, 532
SspI AATATT 1 cut(s) 725
StuI AGGCCT 1 cut(s) 621
StyD4I CCNGG 1 cut(s) 860
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 3 cut(s) 121, 478, 689
TaiI ACGT 1 cut(s) 300
TaqI TCGA 3 cut(s) 225, 279, 796
TaqII GACCGA 1 cut(s) 803
TasI AATT 2 cut(s) 571, 854
TatI WGTACW 1 cut(s) 400
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 750
Tru9I TTAA 1 cut(s) 750
TscAI CASTG 5 cut(s) 431, 483, 654, 685, 903
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 7 cut(s) 8, 125, 128, 354, 357, 813, 869
Tsp45I GTSAC 1 cut(s) 208
TspRI CASTG 5 cut(s) 431, 483, 654, 685, 903
XapI RAATTY 1 cut(s) 854
XceI RCATGY 2 cut(s) 185, 452
XmiI GTMKAC 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.