MD09G1028600.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002928
Physical Location & Seq
Reverse (-)
1774031 .. 1778074
4044 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1028600.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGAAGCGGAAGGCGGGTCCGCAGAAAGCGCAGCACAAATGGAAGAGGAAGCTTTTCACTTTGATTCTGGTGGGGCTCTGCTTCGGAGCGTTGGTGCTGCTGATGCACACTCGCTACAGTAGGATTATGACGCTCGCTTCGTTGCAAAATTCTCAGCAAAATCAACTGCCTAAGATCGCCTTCTTGTTCATCGCTCGCAATCGCATTCCGTTAGAAGTTCTTTGGGATGTTTTCTTTCAGGGAGGGGAGAGTAGGTTTTCGATTTATGTTCACTCGAGGCCGGGGTTTCTGTTCAACAAGGCCACCACAAGATCGGTTTTTTTCTTGAATCGTCAAGTCAATGATAGTATACAGGTAGATTGGGGAGAAGCAAGCATGATTGAAGCAGAGCGTATATTACTCAGACACGCACTCGAGGATCCTCTTAATCAACGCTTTGCCTTTGTTTCTGATAGCTGCATTCCCCTTTACAATTTCAGCTATATATATGACTACATCATGTCAGCGAGAACCAGTTTCGTAGACAGCTTTGCTGATACGAAAGAGGGCCGCTATAATCCAAAAATGCATCCTGCAATTCCTGTTTATAACTGGAGAAAAGGATCACAGTGGGCTGTTTTGACCAGGAAGCATGCTGAGGTTGTAGTGAAAGACGATACAGTCTTTCCTATGTTTCAATTGCACTGCAAGAGAAAATCGCTACCTGAGTTTTGGCGGGATCGTCCCATTCCCGCTGATACATCCAAGGAGCACAATTGTATTCCGGACGAGCATTATGTTCAGACATTACTGGCTCAAGAAGGCCTTGAAGGAGAACTCACACGTAGATCACTGACACACACGTCATGGGATTTATCATCCTCTAGAGACCGTGAACGCCGTGGATGGCACCCTTTGACTTACAAATATTCAGATGCTACTCCCATGCTTATTGAATCTATAAAGGAGATAGATAATGTCTACTACGAGACTGAATACCGGAGAGAATGGTGTAGCAGCAAGGGAAAACCATCTCGGTGCTTTCTTTTTGCTCGAAAATTTACACGGCCTGCTGCTCTCAGGCTTCTTAACACGACTGCACTGGAACTCAACCGGAGCAACGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

369

Amino Acids

43.21

Weight (kDa)

9.55

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 59 - 314 1.1e-69 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 588
AasI GACNNNNNNGTC 2 cut(s) 659, 841
AccB1I GGYRCC 1 cut(s) 888
AccI GTMKAC 3 cut(s) 349, 522, 960
AccIII TCCGGA 1 cut(s) 763
AciI CCGC 6 cut(s) 7, 14, 20, 550, 715, 732
AclWI GGATC 4 cut(s) 413, 426, 610, 726
AcsI RAATTY 2 cut(s) 148, 1037
AflIII ACRYGT 2 cut(s) 821, 840
AgsI TTSAA 6 cut(s) 295, 328, 383, 677, 809, 935
AjiI CACGTC 1 cut(s) 843
AjnI CCWGG 1 cut(s) 623
AluBI AGCT 4 cut(s) 52, 456, 480, 528
AluI AGCT 4 cut(s) 52, 456, 480, 528
Alw21I GWGCWC 1 cut(s) 753
Alw26I GTCTC 2 cut(s) 861, 962
AlwI GGATC 4 cut(s) 413, 426, 610, 726
Ama87I CYCGRG 2 cut(s) 274, 413
Aor13HI TCCGGA 1 cut(s) 763
AoxI GGCC 5 cut(s) 278, 300, 547, 802, 1046
ApeKI GCWGC 5 cut(s) 31, 97, 456, 996, 1052
ApoI RAATTY 2 cut(s) 148, 1037
ArsI GACNNNNNNTTYG 2 cut(s) 121, 153
Asp700I GAANNNNTTC 1 cut(s) 53
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 2 cut(s) 17, 547
AsuC2I CCSGG 1 cut(s) 282
AvaI CYCGRG 2 cut(s) 274, 413
AvaII GGWCC 1 cut(s) 17
BamHI GGATCC 1 cut(s) 418
BanI GGYRCC 1 cut(s) 888
BanII GRGCYC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 753
BbvCI CCTCAGC 1 cut(s) 636
BbvI GCAGC 5 cut(s) 43, 84, 443, 1008, 1039
BccI CCATC 2 cut(s) 879, 1018
BceAI ACGGC 2 cut(s) 864, 1061
BciT130I CCWGG 1 cut(s) 625
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 2 cut(s) 861, 962
BfaI CTAG 1 cut(s) 864
BfmI CTRYAG 1 cut(s) 115
BisI GCNGC 6 cut(s) 32, 98, 457, 550, 997, 1053
BlsI GCNGC 6 cut(s) 33, 99, 458, 551, 998, 1054
Bme1390I CCNGG 2 cut(s) 282, 625
Bme18I GGWCC 1 cut(s) 17
BmeT110I CYCGRG 2 cut(s) 274, 413
BmgBI CACGTC 1 cut(s) 843
BmgT120I GGNCC 2 cut(s) 17, 547
BmiI GGNNCC 3 cut(s) 18, 420, 890
BmrFI CCNGG 2 cut(s) 282, 625
BmsI GCATC 3 cut(s) 93, 577, 904
BpmI CTGGAG 1 cut(s) 613
Bpu10I CCTNAGC 1 cut(s) 636
BpuEI CTTGAG 1 cut(s) 780
BpuMI CCSGG 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 824
BsaI GGTCTC 1 cut(s) 861
BsaJI CCNNGG 3 cut(s) 281, 744, 880
BsaWI WCCGGW 3 cut(s) 763, 978, 1092
BsaXI ACNNNNNCTCC 4 cut(s) 78, 108, 239, 269
Bse1I ACTGG 4 cut(s) 513, 596, 795, 1086
BseAI TCCGGA 1 cut(s) 763
BseBI CCWGG 1 cut(s) 625
BseDI CCNNGG 3 cut(s) 281, 744, 880
BseGI GGATG 5 cut(s) 232, 568, 740, 857, 890
BseMII CTCAG 5 cut(s) 167, 415, 627, 696, 1072
BseNI ACTGG 4 cut(s) 513, 596, 795, 1086
BseXI GCAGC 5 cut(s) 43, 84, 443, 1008, 1039
BsgI GTGCAG 1 cut(s) 1062
BshFI GGCC 5 cut(s) 280, 302, 549, 804, 1048
BshNI GGYRCC 1 cut(s) 888
BsiHKAI GWGCWC 1 cut(s) 753
BsiHKCI CYCGRG 2 cut(s) 274, 413
BsiSI CCGG 4 cut(s) 281, 764, 979, 1093
BslFI GGGAC 1 cut(s) 708
BsmAI GTCTC 2 cut(s) 861, 962
BsmFI GGGAC 1 cut(s) 708
BsmI GAATGC 2 cut(s) 204, 459
BsnI GGCC 5 cut(s) 280, 302, 549, 804, 1048
Bso31I GGTCTC 1 cut(s) 861
BsoBI CYCGRG 2 cut(s) 274, 413
Bsp1286I GDGCHC 2 cut(s) 78, 753
Bsp13I TCCGGA 1 cut(s) 763
Bsp143I GATC 6 cut(s) 174, 311, 418, 602, 718, 827
BspACI CCGC 6 cut(s) 7, 14, 20, 550, 715, 732
BspANI GGCC 5 cut(s) 280, 302, 549, 804, 1048
BspCNI CTCAG 5 cut(s) 166, 414, 628, 697, 1071
BspEI TCCGGA 1 cut(s) 763
BspLI GGNNCC 3 cut(s) 18, 420, 890
BspPI GGATC 4 cut(s) 413, 426, 610, 726
BspT107I GGYRCC 1 cut(s) 888
BspTNI GGTCTC 1 cut(s) 861
BsrI ACTGG 4 cut(s) 513, 596, 795, 1086
BssECI CCNNGG 3 cut(s) 281, 744, 880
BssMI GATC 6 cut(s) 174, 311, 418, 602, 718, 827
BssNAI GTATAC 1 cut(s) 350
BssT1I CCWWGG 1 cut(s) 744
Bst1107I GTATAC 1 cut(s) 350
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 4 cut(s) 119, 609, 661, 872
Bst6I CTCTTC 1 cut(s) 38
BstBAI YACGTR 1 cut(s) 824
BstC8I GCNNGC 5 cut(s) 135, 196, 373, 633, 1050
BstDEI CTNAG 6 cut(s) 153, 171, 401, 636, 705, 1058
BstDSI CCRYGG 1 cut(s) 880
BstF5I GGATG 5 cut(s) 232, 568, 740, 857, 890
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 6 cut(s) 177, 314, 421, 605, 721, 830
BstMAI GTCTC 2 cut(s) 861, 962
BstMBI GATC 6 cut(s) 174, 311, 418, 602, 718, 827
BstMWI GCNNNNNNNGC 2 cut(s) 28, 103
BstNI CCWGG 1 cut(s) 625
BstNSI RCATGY 1 cut(s) 635
BstSCI CCNGG 2 cut(s) 280, 623
BstSFI CTRYAG 1 cut(s) 115
BstV1I GCAGC 5 cut(s) 43, 84, 443, 1008, 1039
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BstZ17I GTATAC 1 cut(s) 350
BsuRI GGCC 5 cut(s) 280, 302, 549, 804, 1048
BtgI CCRYGG 1 cut(s) 880
BtgZI GCGATG 1 cut(s) 175
BtrI CACGTC 1 cut(s) 843
BtsCI GGATG 5 cut(s) 232, 568, 740, 857, 890
BtsI GCAGTG 1 cut(s) 682
BtsIMutI CAGTG 4 cut(s) 614, 682, 830, 1079
Cac8I GCNNGC 5 cut(s) 135, 196, 373, 633, 1050
CfoI GCGC 1 cut(s) 31
Cfr13I GGNCC 2 cut(s) 17, 547
CseI GACGC 1 cut(s) 139
CviAII CATG 5 cut(s) 376, 499, 632, 846, 925
DdeI CTNAG 6 cut(s) 153, 171, 401, 636, 705, 1058
DpnI GATC 6 cut(s) 176, 313, 420, 604, 720, 829
DpnII GATC 6 cut(s) 174, 311, 418, 602, 718, 827
DrdI GACNNNNNNGTC 2 cut(s) 659, 841
DseDI GACNNNNNNGTC 2 cut(s) 659, 841
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 744
Eco147I AGGCCT 1 cut(s) 804
Eco24I GRGCYC 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 861
Eco47I GGWCC 1 cut(s) 17
Eco88I CYCGRG 2 cut(s) 274, 413
EcoRII CCWGG 1 cut(s) 623
EcoT14I CCWWGG 1 cut(s) 744
EcoT22I ATGCAT 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 744
FaeI CATG 5 cut(s) 379, 502, 635, 849, 928
FalI AAGNNNNNCTT 4 cut(s) 164, 196, 789, 821
FaqI GGGAC 1 cut(s) 708
FatI CATG 5 cut(s) 375, 498, 631, 845, 924
FauI CCCGC 3 cut(s) 7, 708, 739
FblI GTMKAC 3 cut(s) 349, 522, 960
Fnu4HI GCNGC 6 cut(s) 32, 98, 457, 550, 997, 1053
FokI GGATG 5 cut(s) 239, 555, 727, 844, 897
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 6 cut(s) 32, 98, 457, 550, 997, 1053
FspBI CTAG 1 cut(s) 864
GlaI GCGC 1 cut(s) 30
GluI GCNGC 6 cut(s) 32, 98, 457, 550, 997, 1053
GsuI CTGGAG 1 cut(s) 613
HaeIII GGCC 5 cut(s) 280, 302, 549, 804, 1048
HapII CCGG 4 cut(s) 281, 764, 979, 1093
HgaI GACGC 1 cut(s) 139
HhaI GCGC 1 cut(s) 31
Hin1II CATG 5 cut(s) 379, 502, 635, 849, 928
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 3 cut(s) 64, 328, 935
HpaII CCGG 4 cut(s) 281, 764, 979, 1093
Hpy166II GTNNAC 5 cut(s) 271, 350, 523, 875, 961
Hpy188I TCNGA 5 cut(s) 86, 404, 451, 783, 913
Hpy188III TCNNGA 4 cut(s) 325, 764, 797, 864
Hpy8I GTNNAC 5 cut(s) 271, 350, 523, 875, 961
HpyAV CCTTC 4 cut(s) 4, 190, 794, 803
HpyCH4III ACNGT 4 cut(s) 119, 609, 661, 872
HpyCH4IV ACGT 2 cut(s) 823, 842
HpyCH4V TGCA 8 cut(s) 106, 145, 459, 568, 575, 682, 687, 1079
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 103
HpyF3I CTNAG 6 cut(s) 153, 171, 401, 636, 705, 1058
HpySE526I ACGT 2 cut(s) 823, 842
Hsp92II CATG 5 cut(s) 379, 502, 635, 849, 928
HspAI GCGC 1 cut(s) 29
Kpn2I TCCGGA 1 cut(s) 763
Kzo9I GATC 6 cut(s) 174, 311, 418, 602, 718, 827
LmnI GCTCC 3 cut(s) 86, 748, 1095
Lsp1109I GCAGC 5 cut(s) 43, 84, 443, 1008, 1039
LweI GCATC 3 cut(s) 93, 577, 904
MaeI CTAG 1 cut(s) 864
MaeII ACGT 2 cut(s) 823, 842
MalI GATC 6 cut(s) 176, 313, 420, 604, 720, 829
MboI GATC 6 cut(s) 174, 311, 418, 602, 718, 827
MboII GAAGA 1 cut(s) 55
MfeI CAATTG 2 cut(s) 677, 754
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 2 cut(s) 78, 753
MluCI AATT 6 cut(s) 148, 472, 576, 677, 754, 1037
MnlI CCTC 8 cut(s) 39, 236, 270, 409, 432, 538, 631, 871
Mph1103I ATGCAT 1 cut(s) 570
MroI TCCGGA 1 cut(s) 763
MroXI GAANNNNTTC 1 cut(s) 53
MseI TTAA 3 cut(s) 426, 1068, 1105
MslI CAYNNNNRTG 1 cut(s) 1015
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 4 cut(s) 281, 764, 979, 1093
MspR9I CCNGG 2 cut(s) 282, 625
MunI CAATTG 2 cut(s) 677, 754
Mva1269I GAATGC 2 cut(s) 204, 459
MvaI CCWGG 1 cut(s) 625
MwoI GCNNNNNNNGC 2 cut(s) 28, 103
NciI CCSGG 1 cut(s) 282
NdeII GATC 6 cut(s) 174, 311, 418, 602, 718, 827
NlaIII CATG 5 cut(s) 379, 502, 635, 849, 928
NlaIV GGNNCC 3 cut(s) 18, 420, 890
NsiI ATGCAT 1 cut(s) 570
NspI RCATGY 1 cut(s) 635
PaeI GCATGC 1 cut(s) 635
PaeR7I CTCGAG 2 cut(s) 274, 413
PceI AGGCCT 1 cut(s) 804
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PctI GAATGC 2 cut(s) 204, 459
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 3 cut(s) 64, 328, 935
PkrI GCNGC 6 cut(s) 33, 99, 458, 551, 998, 1054
Ppu21I YACGTR 1 cut(s) 824
PsiI TTATAA 1 cut(s) 588
Psp6I CCWGG 1 cut(s) 623
PspGI CCWGG 1 cut(s) 623
PspN4I GGNNCC 3 cut(s) 18, 420, 890
PspPI GGNCC 2 cut(s) 17, 547
PspXI VCTCGAGB 2 cut(s) 274, 413
PsuI RGATCY 1 cut(s) 418
RseI CAYNNNNRTG 1 cut(s) 1015
SaqAI TTAA 3 cut(s) 426, 1068, 1105
SatI GCNGC 6 cut(s) 32, 98, 457, 550, 997, 1053
Sau3AI GATC 6 cut(s) 174, 311, 418, 602, 718, 827
Sau96I GGNCC 2 cut(s) 17, 547
ScrFI CCNGG 2 cut(s) 282, 625
SduI GDGCHC 2 cut(s) 78, 753
SfaNI GCATC 3 cut(s) 93, 577, 904
SfcI CTRYAG 1 cut(s) 115
Sfr274I CTCGAG 2 cut(s) 274, 413
SinI GGWCC 1 cut(s) 17
SlaI CTCGAG 2 cut(s) 274, 413
SmiMI CAYNNNNRTG 1 cut(s) 1015
SmlI CTYRAG 3 cut(s) 274, 413, 795
SmoI CTYRAG 3 cut(s) 274, 413, 795
SphI GCATGC 1 cut(s) 635
Sse9I AATT 6 cut(s) 148, 472, 576, 677, 754, 1037
SseBI AGGCCT 1 cut(s) 804
SsiI CCGC 6 cut(s) 7, 14, 20, 550, 715, 732
SspI AATATT 1 cut(s) 908
SspMI CTAG 1 cut(s) 864
StuI AGGCCT 1 cut(s) 804
StyD4I CCNGG 2 cut(s) 280, 623
StyI CCWWGG 1 cut(s) 744
TaaI ACNGT 4 cut(s) 119, 609, 661, 872
TaiI ACGT 2 cut(s) 826, 845
TaqI TCGA 4 cut(s) 260, 275, 414, 1033
TasI AATT 6 cut(s) 148, 472, 576, 677, 754, 1037
TauI GCSGC 1 cut(s) 552
TfiI GAWTC 3 cut(s) 64, 328, 935
Tru1I TTAA 3 cut(s) 426, 1068, 1105
Tru9I TTAA 3 cut(s) 426, 1068, 1105
TscAI CASTG 4 cut(s) 614, 689, 837, 1086
TseI GCWGC 5 cut(s) 31, 97, 456, 996, 1052
TspDTI ATGAA 2 cut(s) 17, 178
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 4 cut(s) 614, 689, 837, 1086
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 2 cut(s) 148, 1037
XbaI TCTAGA 1 cut(s) 863
XceI RCATGY 1 cut(s) 635
XhoI CTCGAG 2 cut(s) 274, 413
XmiI GTMKAC 3 cut(s) 349, 522, 960
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 1 cut(s) 864
Zsp2I ATGCAT 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.