Rh5AG168600

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002928
Physical Location & Seq
Reverse (-)
18512476 .. 18515361
2886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG168600.1

Sequence Viewer

Length: 663 bp
ATGGGCCTCCCCTTCTCAGCCCCTCTCTTCGACCAGGATCCTCTTAATCAACGCTTTGCTTTTGTTTCTGATAGCTGCATCCCTCTTTACAATTTCAACTATATATATGACTATATCATGTCAGCCAGAACCAGTTTTGTAGACAGCTTTGCTGATACAAAAGATGGCCGCTACAATCCAAAAATGGATCCTGTTATTCCTGTACAAAACTGGAGAAAAGGATCTCAGTGGGTTGTATTGACGAGGAAGCATGCAGAGGTTGTAGTGAAAGACGATACAGTGTTTCCTATGTTTCAGTTGTATTGTAAGAGGAAGTCGTTACCTGAGTTTTGGCGGGATCATCCTGTTCCGGCCGATAACTCCAAGGAGCACAATTGCATTCCAGATGAGCATTATGTTCAGGCATTACTGGCTGATGTAGATAATATCTACTATGAGACTGAGTATCGAAGAGAATGGTGTAGCAGCAAGGAGAAACCCTCTCCATGCTTTCTTTTTGCCAGAAAATTTACCCGGCCTGCTGCTCTCAGGCTTCTCAACACGCACGACACAGAGACACCGCGACACCCGTCCAGGAACCGACGTCCTCTTTCGCTGTTCCGACCTTTTGCAGGGGGCCTTACACGCTTACTCAGCACGAGGAACTGGCCAACAGTACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

220

Amino Acids

25.89

Weight (kDa)

8.94

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 9 - 138 3.3e-35 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 586
AccI GTMKAC 1 cut(s) 141
AccII CGCG 1 cut(s) 562
AciI CCGC 3 cut(s) 169, 334, 560
AclWI GGATC 6 cut(s) 32, 45, 182, 195, 229, 345
AcoI YGGCCR 3 cut(s) 166, 351, 647
AcsI RAATTY 1 cut(s) 506
AcyI GRCGYC 1 cut(s) 583
AfaI GTAC 2 cut(s) 204, 657
AfiI CCNNNNNNNGG 1 cut(s) 611
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 2 cut(s) 33, 572
AluBI AGCT 2 cut(s) 75, 147
AluI AGCT 2 cut(s) 75, 147
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 2 cut(s) 431, 548
AlwI GGATC 6 cut(s) 32, 45, 182, 195, 229, 345
AoxI GGCC 6 cut(s) 4, 166, 351, 515, 616, 647
ApeKI GCWGC 3 cut(s) 75, 465, 521
ApoI RAATTY 1 cut(s) 506
AspS9I GGNCC 2 cut(s) 4, 616
AsuC2I CCSGG 1 cut(s) 514
BalI TGGCCA 1 cut(s) 649
BamHI GGATCC 2 cut(s) 37, 187
BauI CACGAG 1 cut(s) 637
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 3 cut(s) 62, 477, 508
BccI CCATC 1 cut(s) 158
BciT130I CCWGG 2 cut(s) 35, 574
BcnI CCSGG 1 cut(s) 514
BcoDI GTCTC 2 cut(s) 431, 548
BisI GCNGC 4 cut(s) 76, 169, 466, 522
BlsI GCNGC 4 cut(s) 77, 170, 467, 523
Bme1390I CCNGG 3 cut(s) 35, 514, 574
BmgT120I GGNCC 2 cut(s) 4, 616
BmiI GGNNCC 4 cut(s) 39, 189, 578, 617
BmrFI CCNGG 3 cut(s) 35, 514, 574
BmsI GCATC 1 cut(s) 87
BoxI GACNNNNGTC 1 cut(s) 568
BplI GAGNNNNNCTC 2 cut(s) 464, 496
BpmI CTGGAG 1 cut(s) 232
BpuMI CCSGG 1 cut(s) 514
BsaHI GRCGYC 1 cut(s) 583
BsaJI CCNNGG 1 cut(s) 363
Bsc4I CCNNNNNNNGG 1 cut(s) 611
Bse1I ACTGG 4 cut(s) 132, 215, 414, 650
BseBI CCWGG 2 cut(s) 35, 574
BseDI CCNNGG 1 cut(s) 363
BseGI GGATG 2 cut(s) 78, 340
BseLI CCNNNNNNNGG 1 cut(s) 611
BseMII CTCAG 6 cut(s) 30, 239, 315, 432, 541, 646
BseNI ACTGG 4 cut(s) 132, 215, 414, 650
BseX3I CGGCCG 1 cut(s) 351
BseXI GCAGC 3 cut(s) 62, 477, 508
Bsh1236I CGCG 1 cut(s) 562
Bsh1285I CGRYCG 1 cut(s) 354
BshFI GGCC 6 cut(s) 6, 168, 353, 517, 618, 649
BsiEI CGRYCG 1 cut(s) 354
BsiHKAI GWGCWC 1 cut(s) 372
BsiSI CCGG 2 cut(s) 350, 514
BslI CCNNNNNNNGG 1 cut(s) 611
BsmAI GTCTC 2 cut(s) 431, 548
BsmI GAATGC 1 cut(s) 378
BsnI GGCC 6 cut(s) 6, 168, 353, 517, 618, 649
Bsp1286I GDGCHC 1 cut(s) 372
Bsp1407I TGTACA 1 cut(s) 202
Bsp143I GATC 4 cut(s) 37, 187, 221, 337
BspACI CCGC 3 cut(s) 169, 334, 560
BspANI GGCC 6 cut(s) 6, 168, 353, 517, 618, 649
BspCNI CTCAG 6 cut(s) 29, 238, 316, 433, 540, 645
BspFNI CGCG 1 cut(s) 562
BspLI GGNNCC 4 cut(s) 39, 189, 578, 617
BspPI GGATC 6 cut(s) 32, 45, 182, 195, 229, 345
BsrGI TGTACA 1 cut(s) 202
BsrI ACTGG 4 cut(s) 132, 215, 414, 650
BssECI CCNNGG 1 cut(s) 363
BssMI GATC 4 cut(s) 37, 187, 221, 337
BssNI GRCGYC 1 cut(s) 583
BssSI CACGAG 1 cut(s) 637
BssT1I CCWWGG 1 cut(s) 363
Bst2BI CACGAG 1 cut(s) 637
Bst2UI CCWGG 2 cut(s) 35, 574
Bst4CI ACNGT 2 cut(s) 280, 655
Bst6I CTCTTC 2 cut(s) 32, 445
BstACI GRCGYC 1 cut(s) 583
BstAUI TGTACA 1 cut(s) 202
BstC8I GCNNGC 2 cut(s) 252, 519
BstDEI CTNAG 6 cut(s) 16, 225, 324, 441, 527, 632
BstENI CCTNNNNNAGG 1 cut(s) 609
BstF5I GGATG 2 cut(s) 78, 340
BstFNI CGCG 1 cut(s) 562
BstKTI GATC 4 cut(s) 40, 190, 224, 340
BstMAI GTCTC 2 cut(s) 431, 548
BstMBI GATC 4 cut(s) 37, 187, 221, 337
BstMCI CGRYCG 1 cut(s) 354
BstMWI GCNNNNNNNGC 3 cut(s) 410, 624, 633
BstNI CCWGG 2 cut(s) 35, 574
BstNSI RCATGY 1 cut(s) 254
BstPAI GACNNNNGTC 1 cut(s) 568
BstSCI CCNGG 3 cut(s) 33, 512, 572
BstUI CGCG 1 cut(s) 562
BstV1I GCAGC 3 cut(s) 62, 477, 508
BstX2I RGATCY 3 cut(s) 37, 187, 221
BstYI RGATCY 3 cut(s) 37, 187, 221
BstZI CGGCCG 1 cut(s) 351
BsuRI GGCC 6 cut(s) 6, 168, 353, 517, 618, 649
BtsCI GGATG 2 cut(s) 78, 340
BtsIMutI CAGTG 2 cut(s) 233, 285
Cac8I GCNNGC 2 cut(s) 252, 519
Cfr13I GGNCC 2 cut(s) 4, 616
Csp6I GTAC 2 cut(s) 203, 656
CviAII CATG 3 cut(s) 118, 251, 486
CviQI GTAC 2 cut(s) 203, 656
DdeI CTNAG 6 cut(s) 16, 225, 324, 441, 527, 632
DpnI GATC 4 cut(s) 39, 189, 223, 339
DpnII GATC 4 cut(s) 37, 187, 221, 337
EaeI YGGCCR 3 cut(s) 166, 351, 647
EagI CGGCCG 1 cut(s) 351
Eam1104I CTCTTC 2 cut(s) 32, 445
EarI CTCTTC 2 cut(s) 32, 445
EclXI CGGCCG 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 363
Eco52I CGGCCG 1 cut(s) 351
EcoNI CCTNNNNNAGG 1 cut(s) 609
EcoO109I RGGNCCY 1 cut(s) 616
EcoRII CCWGG 2 cut(s) 33, 572
EcoT14I CCWWGG 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 3 cut(s) 121, 254, 489
FatI CATG 3 cut(s) 117, 250, 485
FauI CCCGC 1 cut(s) 327
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 4 cut(s) 76, 169, 466, 522
FokI GGATG 2 cut(s) 65, 327
Fsp4HI GCNGC 4 cut(s) 76, 169, 466, 522
GluI GCNGC 4 cut(s) 76, 169, 466, 522
GsuI CTGGAG 1 cut(s) 232
HaeIII GGCC 6 cut(s) 6, 168, 353, 517, 618, 649
HapII CCGG 2 cut(s) 350, 514
Hin1I GRCGYC 1 cut(s) 583
Hin1II CATG 3 cut(s) 121, 254, 489
HpaII CCGG 2 cut(s) 350, 514
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 2 cut(s) 70, 602
Hpy188III TCNNGA 1 cut(s) 383
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 1 cut(s) 585
HpyAV CCTTC 1 cut(s) 22
HpyCH4III ACNGT 2 cut(s) 280, 655
HpyCH4IV ACGT 1 cut(s) 583
HpyCH4V TGCA 4 cut(s) 78, 254, 378, 611
HpyF10VI GCNNNNNNNGC 3 cut(s) 410, 624, 633
HpyF3I CTNAG 6 cut(s) 16, 225, 324, 441, 527, 632
HpySE526I ACGT 1 cut(s) 583
Hsp92I GRCGYC 1 cut(s) 583
Hsp92II CATG 3 cut(s) 121, 254, 489
Kzo9I GATC 4 cut(s) 37, 187, 221, 337
LmnI GCTCC 1 cut(s) 367
Lsp1109I GCAGC 3 cut(s) 62, 477, 508
LweI GCATC 1 cut(s) 87
MaeII ACGT 1 cut(s) 583
MaeIII GTNAC 1 cut(s) 318
MalI GATC 4 cut(s) 39, 189, 223, 339
MboI GATC 4 cut(s) 37, 187, 221, 337
MboII GAAGA 2 cut(s) 19, 462
MfeI CAATTG 1 cut(s) 373
MflI RGATCY 3 cut(s) 37, 187, 221
MhlI GDGCHC 1 cut(s) 372
MlsI TGGCCA 1 cut(s) 649
MluCI AATT 3 cut(s) 91, 373, 506
MluNI TGGCCA 1 cut(s) 649
MmeI TCCRAC 1 cut(s) 625
Mox20I TGGCCA 1 cut(s) 649
MscI TGGCCA 1 cut(s) 649
MseI TTAA 1 cut(s) 45
Msp20I TGGCCA 1 cut(s) 649
MspI CCGG 2 cut(s) 350, 514
MspR9I CCNGG 3 cut(s) 35, 514, 574
MunI CAATTG 1 cut(s) 373
Mva1269I GAATGC 1 cut(s) 378
MvaI CCWGG 2 cut(s) 35, 574
MvnI CGCG 1 cut(s) 562
MwoI GCNNNNNNNGC 3 cut(s) 410, 624, 633
NciI CCSGG 1 cut(s) 514
NdeII GATC 4 cut(s) 37, 187, 221, 337
NlaIII CATG 3 cut(s) 121, 254, 489
NlaIV GGNNCC 4 cut(s) 39, 189, 578, 617
NspI RCATGY 1 cut(s) 254
PaeI GCATGC 1 cut(s) 254
PctI GAATGC 1 cut(s) 378
PfoI TCCNGGA 1 cut(s) 572
PkrI GCNGC 4 cut(s) 77, 170, 467, 523
PshAI GACNNNNGTC 1 cut(s) 568
Psp6I CCWGG 2 cut(s) 33, 572
PspGI CCWGG 2 cut(s) 33, 572
PspN4I GGNNCC 4 cut(s) 39, 189, 578, 617
PspPI GGNCC 2 cut(s) 4, 616
PsuI RGATCY 3 cut(s) 37, 187, 221
RsaI GTAC 2 cut(s) 204, 657
RsaNI GTAC 2 cut(s) 203, 656
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 4 cut(s) 76, 169, 466, 522
Sau3AI GATC 4 cut(s) 37, 187, 221, 337
Sau96I GGNCC 2 cut(s) 4, 616
ScrFI CCNGG 3 cut(s) 35, 514, 574
SduI GDGCHC 1 cut(s) 372
SetI ASST 6 cut(s) 77, 149, 261, 325, 586, 607
SfaNI GCATC 1 cut(s) 87
SphI GCATGC 1 cut(s) 254
Sse9I AATT 3 cut(s) 91, 373, 506
SsiI CCGC 3 cut(s) 169, 334, 560
StyD4I CCNGG 3 cut(s) 33, 512, 572
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 2 cut(s) 280, 655
TaiI ACGT 1 cut(s) 586
TaqI TCGA 2 cut(s) 30, 448
TasI AATT 3 cut(s) 91, 373, 506
TatI WGTACW 2 cut(s) 202, 655
TauI GCSGC 1 cut(s) 171
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 2 cut(s) 233, 285
TseI GCWGC 3 cut(s) 75, 465, 521
TspRI CASTG 2 cut(s) 233, 285
XagI CCTNNNNNAGG 1 cut(s) 609
XapI RAATTY 1 cut(s) 506
XceI RCATGY 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 141
ZraI GACGTC 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.