RchiOBHm_Chr2g0105161

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002928
Physical Location & Seq
Reverse (-)
16471742 .. 16474403
2662 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47938

Sequence Viewer

Length: 594 bp
ATGGATCCTGTTATTCCTGTACAAAACTGGAGAAAAGGATCTCAGTGGGTTGTATTGACGAGGAAGCATGCAGAGGTTGTAGTGAAAGACAATACAGTGTTTCCTATGTTTCAGTTGTATTGTAAGAGGAAGTCGTTACCTGAGTTTTGGCGGGATCATCCTGTTCCGGCCGATAACTCCAAGGAGCACAATTGCATTCCAGACGAGCATTATGTTCAGACATTACTGGCTCAAGAAGGCCTTGAAGGAGAAATCACACGAAGATCACTGACACACTCATCATGGGATTTATCATCCACTGCAGACCGTGAACGCCGTGGATGGCACCCTATGACATACAAATATTCAGATGCTACTCCCATGCTTATTAAATCTATAAAGGATGTAGATAATATCTACTATGAGACCGAGTATCGAAGAGAATGGTGTAGCAGCAAGGAGAAACCCTCTCCATGCTTTCTTTTTGCCAGAAAATTTACCCGGCCTGCTGCTCTCAGGCTTCTCAACACGGTAACATTCAAAATTTTTGTCAATGTTCTAGCTTTTGCTAGGATTTTTTCTAATGTATTTTTGTCAAGGAGTCCCGTAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

197

Amino Acids

23.13

Weight (kDa)

9.3

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 1 - 126 6e-29 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 324
AciI CCGC 1 cut(s) 151
AclWI GGATC 3 cut(s) 12, 46, 162
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 2 cut(s) 473, 522
AfaI GTAC 1 cut(s) 21
AgsI TTSAA 2 cut(s) 245, 520
AluBI AGCT 1 cut(s) 542
AluI AGCT 1 cut(s) 542
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 3 cut(s) 12, 46, 162
AoxI GGCC 3 cut(s) 168, 238, 482
ApeKI GCWGC 2 cut(s) 432, 488
ApoI RAATTY 2 cut(s) 473, 522
ArsI GACNNNNNNTTYG 2 cut(s) 513, 545
AsuC2I CCSGG 1 cut(s) 481
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 444, 475
BccI CCATC 1 cut(s) 315
BceAI ACGGC 1 cut(s) 300
BcnI CCSGG 1 cut(s) 481
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 2 cut(s) 539, 549
BfmI CTRYAG 1 cut(s) 300
BisI GCNGC 2 cut(s) 433, 489
BlsI GCNGC 2 cut(s) 434, 490
Bme1390I CCNGG 1 cut(s) 481
BmiI GGNNCC 2 cut(s) 6, 326
BmrFI CCNGG 1 cut(s) 481
BmsI GCATC 1 cut(s) 340
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpmI CTGGAG 1 cut(s) 49
BpuEI CTTGAG 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 481
BsaI GGTCTC 1 cut(s) 398
BsaJI CCNNGG 2 cut(s) 180, 316
Bse1I ACTGG 2 cut(s) 32, 231
BseDI CCNNGG 2 cut(s) 180, 316
BseGI GGATG 4 cut(s) 157, 293, 326, 388
BseMII CTCAG 3 cut(s) 56, 132, 508
BseNI ACTGG 2 cut(s) 32, 231
BseX3I CGGCCG 1 cut(s) 168
BseXI GCAGC 2 cut(s) 444, 475
Bsh1285I CGRYCG 1 cut(s) 171
BshFI GGCC 3 cut(s) 170, 240, 484
BshNI GGYRCC 1 cut(s) 324
BsiEI CGRYCG 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 2 cut(s) 167, 481
BslFI GGGAC 1 cut(s) 567
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 567
BsmI GAATGC 1 cut(s) 195
BsnI GGCC 3 cut(s) 170, 240, 484
Bso31I GGTCTC 1 cut(s) 398
Bsp1286I GDGCHC 1 cut(s) 189
Bsp1407I TGTACA 1 cut(s) 19
Bsp143I GATC 4 cut(s) 4, 38, 154, 263
BspACI CCGC 1 cut(s) 151
BspANI GGCC 3 cut(s) 170, 240, 484
BspCNI CTCAG 3 cut(s) 55, 133, 507
BspLI GGNNCC 2 cut(s) 6, 326
BspMAI CTGCAG 1 cut(s) 304
BspPI GGATC 3 cut(s) 12, 46, 162
BspT107I GGYRCC 1 cut(s) 324
BspTNI GGTCTC 1 cut(s) 398
BsrGI TGTACA 1 cut(s) 19
BsrI ACTGG 2 cut(s) 32, 231
BssECI CCNNGG 2 cut(s) 180, 316
BssMI GATC 4 cut(s) 4, 38, 154, 263
BssT1I CCWWGG 1 cut(s) 180
Bst4CI ACNGT 3 cut(s) 97, 308, 511
Bst6I CTCTTC 1 cut(s) 412
BstAUI TGTACA 1 cut(s) 19
BstC8I GCNNGC 2 cut(s) 69, 486
BstDEI CTNAG 3 cut(s) 42, 141, 494
BstDSI CCRYGG 1 cut(s) 316
BstF5I GGATG 4 cut(s) 157, 293, 326, 388
BstKTI GATC 4 cut(s) 7, 41, 157, 266
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 4 cut(s) 4, 38, 154, 263
BstMCI CGRYCG 1 cut(s) 171
BstNSI RCATGY 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 479
BstSFI CTRYAG 1 cut(s) 300
BstV1I GCAGC 2 cut(s) 444, 475
BstX2I RGATCY 2 cut(s) 4, 38
BstYI RGATCY 2 cut(s) 4, 38
BstZI CGGCCG 1 cut(s) 168
BsuRI GGCC 3 cut(s) 170, 240, 484
BtgI CCRYGG 1 cut(s) 316
BtsCI GGATG 4 cut(s) 157, 293, 326, 388
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 4 cut(s) 50, 102, 266, 297
Cac8I GCNNGC 2 cut(s) 69, 486
Csp6I GTAC 1 cut(s) 20
CviAII CATG 4 cut(s) 68, 282, 361, 453
CviJI RGCY 6 cut(s) 170, 230, 240, 484, 499, 542
CviKI_1 RGCY 6 cut(s) 170, 230, 240, 484, 499, 542
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 3 cut(s) 42, 141, 494
DpnI GATC 4 cut(s) 6, 40, 156, 265
DpnII GATC 4 cut(s) 4, 38, 154, 263
EaeI YGGCCR 1 cut(s) 168
EagI CGGCCG 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 412
EarI CTCTTC 1 cut(s) 412
EclXI CGGCCG 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 180
Eco147I AGGCCT 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 398
Eco52I CGGCCG 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 4 cut(s) 71, 285, 364, 456
FalI AAGNNNNNCTT 2 cut(s) 225, 257
FaqI GGGAC 1 cut(s) 567
FatI CATG 4 cut(s) 67, 281, 360, 452
FauI CCCGC 1 cut(s) 144
Fnu4HI GCNGC 2 cut(s) 433, 489
FokI GGATG 4 cut(s) 144, 280, 333, 395
Fsp4HI GCNGC 2 cut(s) 433, 489
FspBI CTAG 2 cut(s) 539, 549
GluI GCNGC 2 cut(s) 433, 489
GsuI CTGGAG 1 cut(s) 49
HaeIII GGCC 3 cut(s) 170, 240, 484
HapII CCGG 2 cut(s) 167, 481
Hin1II CATG 4 cut(s) 71, 285, 364, 456
HinfI GANTC 1 cut(s) 580
HpaII CCGG 2 cut(s) 167, 481
Hpy166II GTNNAC 1 cut(s) 311
Hpy188I TCNGA 2 cut(s) 219, 349
Hpy188III TCNNGA 2 cut(s) 200, 233
Hpy8I GTNNAC 1 cut(s) 311
HpyAV CCTTC 2 cut(s) 230, 239
HpyCH4III ACNGT 3 cut(s) 97, 308, 511
HpyCH4V TGCA 3 cut(s) 71, 195, 302
HpyF3I CTNAG 3 cut(s) 42, 141, 494
Hsp92II CATG 4 cut(s) 71, 285, 364, 456
Kzo9I GATC 4 cut(s) 4, 38, 154, 263
LmnI GCTCC 1 cut(s) 184
Lsp1109I GCAGC 2 cut(s) 444, 475
LweI GCATC 1 cut(s) 340
MaeI CTAG 2 cut(s) 539, 549
MaeIII GTNAC 2 cut(s) 135, 511
MalI GATC 4 cut(s) 6, 40, 156, 265
MboI GATC 4 cut(s) 4, 38, 154, 263
MboII GAAGA 2 cut(s) 273, 429
MfeI CAATTG 1 cut(s) 190
MflI RGATCY 2 cut(s) 4, 38
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 3 cut(s) 190, 473, 522
MlyI GAGTC 1 cut(s) 589
MnlI CCTC 4 cut(s) 54, 67, 120, 457
MseI TTAA 1 cut(s) 369
MspI CCGG 2 cut(s) 167, 481
MspR9I CCNGG 1 cut(s) 481
MunI CAATTG 1 cut(s) 190
Mva1269I GAATGC 1 cut(s) 195
NciI CCSGG 1 cut(s) 481
NdeII GATC 4 cut(s) 4, 38, 154, 263
NlaIII CATG 4 cut(s) 71, 285, 364, 456
NlaIV GGNNCC 2 cut(s) 6, 326
NspI RCATGY 1 cut(s) 71
PaeI GCATGC 1 cut(s) 71
PceI AGGCCT 1 cut(s) 240
PctI GAATGC 1 cut(s) 195
PkrI GCNGC 2 cut(s) 434, 490
PleI GAGTC 1 cut(s) 588
PpsI GAGTC 1 cut(s) 588
PspN4I GGNNCC 2 cut(s) 6, 326
PstI CTGCAG 1 cut(s) 304
PsuI RGATCY 2 cut(s) 4, 38
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 2 cut(s) 433, 489
Sau3AI GATC 4 cut(s) 4, 38, 154, 263
SchI GAGTC 1 cut(s) 589
ScrFI CCNGG 1 cut(s) 481
SduI GDGCHC 1 cut(s) 189
SetI ASST 3 cut(s) 78, 142, 544
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 300
SmlI CTYRAG 1 cut(s) 231
SmoI CTYRAG 1 cut(s) 231
SphI GCATGC 1 cut(s) 71
Sse9I AATT 3 cut(s) 190, 473, 522
SseBI AGGCCT 1 cut(s) 240
SsiI CCGC 1 cut(s) 151
SspI AATATT 1 cut(s) 344
SspMI CTAG 2 cut(s) 539, 549
StuI AGGCCT 1 cut(s) 240
StyD4I CCNGG 1 cut(s) 479
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 3 cut(s) 97, 308, 511
TaqI TCGA 1 cut(s) 415
TaqII GACCGA 1 cut(s) 422
TasI AATT 3 cut(s) 190, 473, 522
TatI WGTACW 1 cut(s) 19
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 4 cut(s) 50, 102, 273, 304
TseI GCWGC 2 cut(s) 432, 488
TspRI CASTG 4 cut(s) 50, 102, 273, 304
XapI RAATTY 2 cut(s) 473, 522
XceI RCATGY 1 cut(s) 71
XspI CTAG 2 cut(s) 539, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.