Rroxscaffold_2G00079270

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002928
Physical Location & Seq
Forward (+)
2294640 .. 2299181
4542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00079270.1

Sequence Viewer

Length: 597 bp
ATGTCAACCAGAACCAGTTTTGTAGACAGCTTTGCTGATACAAAAGATGGCCGCTACAATCCAAAAATGGATCCTGTTATTCCTGTACAAAACTGGAGAAAAGGATCTCAGTGGGTTGTATTGACGAGGAAGCATGCAGAGGTTGTAGTGAAAGACGATACAGTGTTTCCTATGTTTCAGTGGTATTGCCAGAGGAAGTCGTTACCTGAGTTTTGGCGGGATCGTCCTGTTCCAGCCGATAACTCCAAGGAGCACAATTGCATTCCGGACGAGCATTATGTTCAGACATTATTGGCTCAAGAAGGCCTTGAAGGAGAAATCACACGAAGATCACTGACACACTCATCATGGGATTTATCATCCACTGGAGACCGTGAACGCCGTGGATGGCACCCTATGACATACAAATATTCAGATGCTACTCCCATGCTTATTAAATCTATAAAGGATGTAGATAATATCTACTATGAGACCGAGTATCGAAGAGAATGGTGTAGCAGCAAGGGGAAACCCTCTCCATGCTTTCTTTTTGCCAGAAAATTTACCCGGCCTGCTGCTCTCAGGCTTCTCAACACGACTACACTGGAGCTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

198

Amino Acids

23.34

Weight (kDa)

9.01

Isoelectric Point (pI)

40.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 3 - 148 1.6e-34 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 390
AccI GTMKAC 1 cut(s) 24
AccIII TCCGGA 1 cut(s) 265
AciI CCGC 2 cut(s) 52, 217
AclWI GGATC 4 cut(s) 65, 78, 112, 228
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 1 cut(s) 539
AfaI GTAC 1 cut(s) 87
AgsI TTSAA 1 cut(s) 311
AluBI AGCT 2 cut(s) 30, 589
AluI AGCT 2 cut(s) 30, 589
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 2 cut(s) 363, 464
AlwI GGATC 4 cut(s) 65, 78, 112, 228
Aor13HI TCCGGA 1 cut(s) 265
AoxI GGCC 3 cut(s) 49, 304, 548
ApeKI GCWGC 2 cut(s) 498, 554
ApoI RAATTY 1 cut(s) 539
AsuC2I CCSGG 1 cut(s) 547
BamHI GGATCC 1 cut(s) 70
BanI GGYRCC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 2 cut(s) 510, 541
BccI CCATC 2 cut(s) 41, 381
BceAI ACGGC 1 cut(s) 366
BcnI CCSGG 1 cut(s) 547
BcoDI GTCTC 2 cut(s) 363, 464
BisI GCNGC 3 cut(s) 52, 499, 555
BlsI GCNGC 3 cut(s) 53, 500, 556
Bme1390I CCNGG 1 cut(s) 547
BmiI GGNNCC 2 cut(s) 72, 392
BmrFI CCNGG 1 cut(s) 547
BmsI GCATC 1 cut(s) 406
BpmI CTGGAG 2 cut(s) 115, 387
BpuEI CTTGAG 1 cut(s) 282
BpuMI CCSGG 1 cut(s) 547
BsaI GGTCTC 2 cut(s) 363, 464
BsaJI CCNNGG 2 cut(s) 246, 382
BsaWI WCCGGW 1 cut(s) 265
Bse1I ACTGG 4 cut(s) 15, 98, 370, 588
BseAI TCCGGA 1 cut(s) 265
BseDI CCNNGG 2 cut(s) 246, 382
BseGI GGATG 3 cut(s) 359, 392, 454
BseMII CTCAG 3 cut(s) 122, 198, 574
BseNI ACTGG 4 cut(s) 15, 98, 370, 588
BseXI GCAGC 2 cut(s) 510, 541
BshFI GGCC 3 cut(s) 51, 306, 550
BshNI GGYRCC 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 2 cut(s) 266, 547
BsmAI GTCTC 2 cut(s) 363, 464
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 3 cut(s) 51, 306, 550
Bso31I GGTCTC 2 cut(s) 363, 464
Bsp1286I GDGCHC 1 cut(s) 255
Bsp13I TCCGGA 1 cut(s) 265
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 4 cut(s) 70, 104, 220, 329
BspACI CCGC 2 cut(s) 52, 217
BspANI GGCC 3 cut(s) 51, 306, 550
BspCNI CTCAG 3 cut(s) 121, 199, 573
BspEI TCCGGA 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 72, 392
BspPI GGATC 4 cut(s) 65, 78, 112, 228
BspT107I GGYRCC 1 cut(s) 390
BspTNI GGTCTC 2 cut(s) 363, 464
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 4 cut(s) 15, 98, 370, 588
BssECI CCNNGG 2 cut(s) 246, 382
BssMI GATC 4 cut(s) 70, 104, 220, 329
BssT1I CCWWGG 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 163, 374
Bst6I CTCTTC 1 cut(s) 478
BstAUI TGTACA 1 cut(s) 85
BstC8I GCNNGC 2 cut(s) 135, 552
BstDEI CTNAG 4 cut(s) 108, 207, 560, 590
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 3 cut(s) 359, 392, 454
BstKTI GATC 4 cut(s) 73, 107, 223, 332
BstMAI GTCTC 2 cut(s) 363, 464
BstMBI GATC 4 cut(s) 70, 104, 220, 329
BstNSI RCATGY 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 545
BstV1I GCAGC 2 cut(s) 510, 541
BstX2I RGATCY 2 cut(s) 70, 104
BstYI RGATCY 2 cut(s) 70, 104
BsuRI GGCC 3 cut(s) 51, 306, 550
BtgI CCRYGG 1 cut(s) 382
BtsCI GGATG 3 cut(s) 359, 392, 454
BtsIMutI CAGTG 6 cut(s) 116, 168, 185, 332, 363, 581
Cac8I GCNNGC 2 cut(s) 135, 552
Csp6I GTAC 1 cut(s) 86
CviAII CATG 4 cut(s) 134, 348, 427, 519
CviJI RGCY 8 cut(s) 30, 51, 236, 296, 306, 550, 565, 589
CviKI_1 RGCY 8 cut(s) 30, 51, 236, 296, 306, 550, 565, 589
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 4 cut(s) 108, 207, 560, 590
DpnI GATC 4 cut(s) 72, 106, 222, 331
DpnII GATC 4 cut(s) 70, 104, 220, 329
EaeI YGGCCR 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 478
EarI CTCTTC 1 cut(s) 478
Eco130I CCWWGG 1 cut(s) 246
Eco147I AGGCCT 1 cut(s) 306
Eco31I GGTCTC 2 cut(s) 363, 464
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 4 cut(s) 137, 351, 430, 522
FalI AAGNNNNNCTT 2 cut(s) 291, 323
FatI CATG 4 cut(s) 133, 347, 426, 518
FauI CCCGC 1 cut(s) 210
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 3 cut(s) 52, 499, 555
FokI GGATG 3 cut(s) 346, 399, 461
Fsp4HI GCNGC 3 cut(s) 52, 499, 555
GluI GCNGC 3 cut(s) 52, 499, 555
GsuI CTGGAG 2 cut(s) 115, 387
HaeIII GGCC 3 cut(s) 51, 306, 550
HapII CCGG 2 cut(s) 266, 547
Hin1II CATG 4 cut(s) 137, 351, 430, 522
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HpaII CCGG 2 cut(s) 266, 547
Hpy166II GTNNAC 3 cut(s) 6, 25, 377
Hpy188I TCNGA 2 cut(s) 285, 415
Hpy188III TCNNGA 2 cut(s) 266, 299
Hpy8I GTNNAC 3 cut(s) 6, 25, 377
HpyAV CCTTC 2 cut(s) 296, 305
HpyCH4III ACNGT 2 cut(s) 163, 374
HpyCH4V TGCA 2 cut(s) 137, 261
HpyF3I CTNAG 4 cut(s) 108, 207, 560, 590
Hsp92II CATG 4 cut(s) 137, 351, 430, 522
Kpn2I TCCGGA 1 cut(s) 265
Kzo9I GATC 4 cut(s) 70, 104, 220, 329
LmnI GCTCC 2 cut(s) 250, 586
Lsp1109I GCAGC 2 cut(s) 510, 541
LweI GCATC 1 cut(s) 406
MaeIII GTNAC 1 cut(s) 201
MalI GATC 4 cut(s) 72, 106, 222, 331
MboI GATC 4 cut(s) 70, 104, 220, 329
MboII GAAGA 2 cut(s) 339, 495
MfeI CAATTG 1 cut(s) 256
MflI RGATCY 2 cut(s) 70, 104
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 2 cut(s) 256, 539
MnlI CCTC 4 cut(s) 120, 133, 186, 523
MroI TCCGGA 1 cut(s) 265
MseI TTAA 1 cut(s) 435
MspI CCGG 2 cut(s) 266, 547
MspR9I CCNGG 1 cut(s) 547
MunI CAATTG 1 cut(s) 256
Mva1269I GAATGC 1 cut(s) 261
NciI CCSGG 1 cut(s) 547
NdeII GATC 4 cut(s) 70, 104, 220, 329
NlaIII CATG 4 cut(s) 137, 351, 430, 522
NlaIV GGNNCC 2 cut(s) 72, 392
NspI RCATGY 1 cut(s) 137
PaeI GCATGC 1 cut(s) 137
PceI AGGCCT 1 cut(s) 306
PctI GAATGC 1 cut(s) 261
PkrI GCNGC 3 cut(s) 53, 500, 556
PspN4I GGNNCC 2 cut(s) 72, 392
PsuI RGATCY 2 cut(s) 70, 104
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 3 cut(s) 52, 499, 555
Sau3AI GATC 4 cut(s) 70, 104, 220, 329
ScrFI CCNGG 1 cut(s) 547
SduI GDGCHC 1 cut(s) 255
SetI ASST 4 cut(s) 32, 144, 208, 591
SfaNI GCATC 1 cut(s) 406
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
SphI GCATGC 1 cut(s) 137
Sse9I AATT 2 cut(s) 256, 539
SseBI AGGCCT 1 cut(s) 306
SsiI CCGC 2 cut(s) 52, 217
SspI AATATT 1 cut(s) 410
StuI AGGCCT 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 545
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 2 cut(s) 163, 374
TaqI TCGA 1 cut(s) 481
TaqII GACCGA 1 cut(s) 488
TasI AATT 2 cut(s) 256, 539
TatI WGTACW 1 cut(s) 85
TauI GCSGC 1 cut(s) 54
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 6 cut(s) 116, 168, 185, 339, 370, 588
TseI GCWGC 2 cut(s) 498, 554
TspRI CASTG 6 cut(s) 116, 168, 185, 339, 370, 588
XapI RAATTY 1 cut(s) 539
XceI RCATGY 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.