FvH4_6g51390

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002928
Physical Location & Seq
Reverse (-)
38093019 .. 38097496
4478 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g51390.t1

Sequence Viewer

Length: 1077 bp
ATGAAGCGAGCAACAAAGTCACAGTTCAAATGGAAGCGCAAGCTTTTGATGTGCTTGTGCTTTGGGACCTTGGTCCTTCTTATGCACACTCACTACTCTCGGATTATGACTCTCGCTTCAATTCGTCCCCAGATGAATACAATCCAACGGCCCAAGATTGCCTTCTTGTTCATCGCACGCAATCGGTTGCCGTTGGATATGCTCTGGGATGTGTTCTTTCAGGGTGGAGAGAGCAAGTTTTCGATTTATGTGCACTCCAGGCCCGGGTTTCTGTTCAATAAGGTCACCACCAGATCGGATTTTTTCCTGAATCGTCAAGTCAATGATAGCATACAGGTAGATTGGGGAGAGGCGACCATGATTGAAGCAGAGAGGATATTGCTCAAACATGCGCTCGAGGATCCTCTTAATCAACGGTTTGCGTTTGTCTCTGACAGCTGCATCCCGCTTTACAGTTTCAAGTATATATATGACTATGTCATGTCAACAAGAACCAGTTTTGTAGACAGCTTTGCTGATACAAAAGATGGCCGTTACAATCCAAAAATGGATCCTATTATTCCTGTACAGAACTGGAGAAAAGGATCTCAGTGGGCTGTGTTGACCAGGAAGCATGCAGAGGTTGTAGTGAAAGACAATACAGTGTTTCCTATGTTTCAGTTGTATTGCAAGAGGAAGTCTTTACCTGAATTTTGGCGGGATCGCCCTGTTCCGGCTGATACCTCCAAGGAGCACAATTGCATTCCTGATGAGCATTATGTTCAGACATTACTTGCTCAAGAAGGCCTTGAAGGAGAAATCACGCGAAGATCACTGACACACTCATCATGGGATCTAACATCCACAAGAGATCGTGAACGCCGTGGATGGCACCCTATGACCTACAAATATTCAGATGCTACTCCAATGTTGATTAAATCTATAAAGGATATCGATAATATCTACTATGAAACTGAGTATCGAAGAGAATGGTGTAGCAGCAAGGGGAAACCCTCTCCGTGCTTTCTCTTTGCGAGAAAATTTACCCGTCCTGCTGCTCTTAGGCTTCTCAACACAACTACACTGGAGCTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

359

Amino Acids

42.33

Weight (kDa)

9.57

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 53 - 308 6.6e-73 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 870
AccI GTMKAC 1 cut(s) 504
AccII CGCG 1 cut(s) 805
AciI CCGC 2 cut(s) 446, 697
AclWI GGATC 7 cut(s) 395, 408, 545, 558, 592, 708, 840
AcoI YGGCCR 1 cut(s) 529
AcsI RAATTY 2 cut(s) 689, 1019
AfaI GTAC 1 cut(s) 567
AfiI CCNNNNNNNGG 2 cut(s) 264, 712
AgsI TTSAA 6 cut(s) 28, 120, 277, 365, 460, 791
AjnI CCWGG 2 cut(s) 257, 605
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 4 cut(s) 43, 438, 510, 1069
AluI AGCT 4 cut(s) 43, 438, 510, 1069
Alw21I GWGCWC 2 cut(s) 255, 735
Alw26I GTCTC 1 cut(s) 433
Alw44I GTGCAC 1 cut(s) 251
AlwI GGATC 7 cut(s) 395, 408, 545, 558, 592, 708, 840
Ama87I CYCGRG 2 cut(s) 263, 395
AoxI GGCC 4 cut(s) 149, 260, 529, 784
ApaLI GTGCAC 1 cut(s) 251
ApeKI GCWGC 3 cut(s) 438, 978, 1034
ApoI RAATTY 2 cut(s) 689, 1019
AspLEI GCGC 2 cut(s) 39, 394
AspS9I GGNCC 4 cut(s) 66, 73, 150, 261
AsuC2I CCSGG 2 cut(s) 264, 265
AsuHPI GGTGA 1 cut(s) 277
AvaI CYCGRG 2 cut(s) 263, 395
AvaII GGWCC 2 cut(s) 66, 73
BaeGI GKGCMC 1 cut(s) 255
BamHI GGATCC 2 cut(s) 400, 550
BanI GGYRCC 1 cut(s) 870
Bbv12I GWGCWC 2 cut(s) 255, 735
BbvI GCAGC 3 cut(s) 425, 990, 1021
BccI CCATC 2 cut(s) 521, 861
BceAI ACGGC 4 cut(s) 164, 175, 516, 846
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BciT130I CCWGG 2 cut(s) 259, 607
BcnI CCSGG 2 cut(s) 264, 265
BcoDI GTCTC 1 cut(s) 433
BisI GCNGC 3 cut(s) 439, 979, 1035
BlsI GCNGC 3 cut(s) 440, 980, 1036
Bme1390I CCNGG 4 cut(s) 259, 264, 265, 607
Bme18I GGWCC 2 cut(s) 66, 73
BmeT110I CYCGRG 2 cut(s) 263, 395
BmgT120I GGNCC 4 cut(s) 66, 73, 150, 261
BmiI GGNNCC 4 cut(s) 67, 402, 552, 872
BmrFI CCNGG 4 cut(s) 259, 264, 265, 607
BmsI GCATC 2 cut(s) 450, 886
BoxI GACNNNNGTC 1 cut(s) 71
BpmI CTGGAG 2 cut(s) 241, 595
BpuEI CTTGAG 1 cut(s) 762
BpuMI CCSGG 2 cut(s) 264, 265
Bsa29I ATCGAT 1 cut(s) 933
BsaJI CCNNGG 4 cut(s) 69, 263, 726, 862
Bsc4I CCNNNNNNNGG 2 cut(s) 264, 712
Bse1I ACTGG 3 cut(s) 495, 578, 1068
BseBI CCWGG 2 cut(s) 259, 607
BseCI ATCGAT 1 cut(s) 933
BseDI CCNNGG 4 cut(s) 69, 263, 726, 862
BseGI GGATG 4 cut(s) 214, 441, 839, 872
BseLI CCNNNNNNNGG 2 cut(s) 264, 712
BseMII CTCAG 2 cut(s) 602, 945
BseNI ACTGG 3 cut(s) 495, 578, 1068
BseSI GKGCMC 1 cut(s) 255
BseXI GCAGC 3 cut(s) 425, 990, 1021
Bsh1236I CGCG 1 cut(s) 805
BshFI GGCC 4 cut(s) 151, 262, 531, 786
BshNI GGYRCC 1 cut(s) 870
BshVI ATCGAT 1 cut(s) 933
BsiHKAI GWGCWC 2 cut(s) 255, 735
BsiHKCI CYCGRG 2 cut(s) 263, 395
BsiSI CCGG 2 cut(s) 264, 713
BslFI GGGAC 2 cut(s) 79, 111
BslI CCNNNNNNNGG 2 cut(s) 264, 712
BsmAI GTCTC 1 cut(s) 433
BsmFI GGGAC 2 cut(s) 79, 111
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 4 cut(s) 151, 262, 531, 786
BsoBI CYCGRG 2 cut(s) 263, 395
Bsp1286I GDGCHC 2 cut(s) 255, 735
Bsp1407I TGTACA 1 cut(s) 565
Bsp143I GATC 8 cut(s) 293, 400, 550, 584, 700, 809, 832, 850
BspACI CCGC 2 cut(s) 446, 697
BspANI GGCC 4 cut(s) 151, 262, 531, 786
BspCNI CTCAG 2 cut(s) 601, 946
BspDI ATCGAT 1 cut(s) 933
BspFNI CGCG 1 cut(s) 805
BspLI GGNNCC 4 cut(s) 67, 402, 552, 872
BspPI GGATC 7 cut(s) 395, 408, 545, 558, 592, 708, 840
BspT107I GGYRCC 1 cut(s) 870
BsrGI TGTACA 1 cut(s) 565
BsrI ACTGG 3 cut(s) 495, 578, 1068
BssECI CCNNGG 4 cut(s) 69, 263, 726, 862
BssMI GATC 8 cut(s) 293, 400, 550, 584, 700, 809, 832, 850
BssT1I CCWWGG 2 cut(s) 69, 726
Bst2UI CCWGG 2 cut(s) 259, 607
Bst4CI ACNGT 4 cut(s) 24, 417, 455, 643
Bst6I CTCTTC 1 cut(s) 958
BstAUI TGTACA 1 cut(s) 565
BstC8I GCNNGC 4 cut(s) 9, 41, 178, 615
BstDEI CTNAG 4 cut(s) 588, 954, 1040, 1070
BstDSI CCRYGG 1 cut(s) 862
BstEII GGTNACC 1 cut(s) 283
BstF5I GGATG 4 cut(s) 214, 441, 839, 872
BstFNI CGCG 1 cut(s) 805
BstHHI GCGC 2 cut(s) 39, 394
BstKTI GATC 8 cut(s) 296, 403, 553, 587, 703, 812, 835, 853
BstMAI GTCTC 1 cut(s) 433
BstMBI GATC 8 cut(s) 293, 400, 550, 584, 700, 809, 832, 850
BstMWI GCNNNNNNNGC 1 cut(s) 259
BstNI CCWGG 2 cut(s) 259, 607
BstNSI RCATGY 2 cut(s) 392, 617
BstPAI GACNNNNGTC 1 cut(s) 71
BstPI GGTNACC 1 cut(s) 283
BstSCI CCNGG 4 cut(s) 257, 262, 263, 605
BstSLI GKGCMC 1 cut(s) 255
BstUI CGCG 1 cut(s) 805
BstV1I GCAGC 3 cut(s) 425, 990, 1021
BstX2I RGATCY 4 cut(s) 400, 550, 584, 832
BstYI RGATCY 4 cut(s) 400, 550, 584, 832
Bsu15I ATCGAT 1 cut(s) 933
BsuRI GGCC 4 cut(s) 151, 262, 531, 786
BsuTUI ATCGAT 1 cut(s) 933
BtgI CCRYGG 1 cut(s) 862
BtgZI GCGATG 1 cut(s) 157
BtsCI GGATG 4 cut(s) 214, 441, 839, 872
BtsIMutI CAGTG 4 cut(s) 596, 648, 812, 1061
Cac8I GCNNGC 4 cut(s) 9, 41, 178, 615
CfoI GCGC 2 cut(s) 39, 394
Cfr13I GGNCC 4 cut(s) 66, 73, 150, 261
Cfr9I CCCGGG 1 cut(s) 263
ClaI ATCGAT 1 cut(s) 933
Csp6I GTAC 1 cut(s) 566
CviAII CATG 5 cut(s) 358, 389, 481, 614, 828
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 4 cut(s) 588, 954, 1040, 1070
DpnI GATC 8 cut(s) 295, 402, 552, 586, 702, 811, 834, 852
DpnII GATC 8 cut(s) 293, 400, 550, 584, 700, 809, 832, 850
EaeI YGGCCR 1 cut(s) 529
Eam1104I CTCTTC 1 cut(s) 958
EarI CTCTTC 1 cut(s) 958
Eco130I CCWWGG 2 cut(s) 69, 726
Eco147I AGGCCT 1 cut(s) 786
Eco32I GATATC 1 cut(s) 931
Eco47I GGWCC 2 cut(s) 66, 73
Eco88I CYCGRG 2 cut(s) 263, 395
Eco91I GGTNACC 1 cut(s) 283
EcoO109I RGGNCCY 1 cut(s) 66
EcoO65I GGTNACC 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 257, 605
EcoRV GATATC 1 cut(s) 931
EcoT14I CCWWGG 2 cut(s) 69, 726
ErhI CCWWGG 2 cut(s) 69, 726
FaeI CATG 5 cut(s) 361, 392, 484, 617, 831
FalI AAGNNNNNCTT 4 cut(s) 146, 178, 771, 803
FaqI GGGAC 2 cut(s) 79, 111
FatI CATG 5 cut(s) 357, 388, 480, 613, 827
FauI CCCGC 2 cut(s) 453, 690
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 3 cut(s) 439, 979, 1035
FokI GGATG 4 cut(s) 221, 428, 826, 879
Fsp4HI GCNGC 3 cut(s) 439, 979, 1035
GlaI GCGC 2 cut(s) 38, 393
GluI GCNGC 3 cut(s) 439, 979, 1035
GsuI CTGGAG 2 cut(s) 241, 595
HaeIII GGCC 4 cut(s) 151, 262, 531, 786
HapII CCGG 2 cut(s) 264, 713
HhaI GCGC 2 cut(s) 39, 394
Hin1II CATG 5 cut(s) 361, 392, 484, 617, 831
Hin6I GCGC 2 cut(s) 37, 392
HinP1I GCGC 2 cut(s) 37, 392
HincII GTYRAC 2 cut(s) 486, 603
HindII GTYRAC 2 cut(s) 486, 603
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 2 cut(s) 109, 310
HpaII CCGG 2 cut(s) 264, 713
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 5 cut(s) 253, 486, 505, 603, 857
Hpy188I TCNGA 5 cut(s) 102, 298, 433, 765, 895
Hpy188III TCNNGA 4 cut(s) 307, 746, 779, 854
Hpy8I GTNNAC 5 cut(s) 253, 486, 505, 603, 857
HpyAV CCTTC 4 cut(s) 86, 172, 776, 785
HpyCH4III ACNGT 4 cut(s) 24, 417, 455, 643
HpyCH4V TGCA 6 cut(s) 85, 253, 441, 617, 669, 741
HpyF10VI GCNNNNNNNGC 1 cut(s) 259
HpyF3I CTNAG 4 cut(s) 588, 954, 1040, 1070
Hsp92II CATG 5 cut(s) 361, 392, 484, 617, 831
HspAI GCGC 2 cut(s) 37, 392
Kzo9I GATC 8 cut(s) 293, 400, 550, 584, 700, 809, 832, 850
LmnI GCTCC 2 cut(s) 730, 1066
Lsp1109I GCAGC 3 cut(s) 425, 990, 1021
LweI GCATC 2 cut(s) 450, 886
MaeIII GTNAC 3 cut(s) 18, 283, 533
MalI GATC 8 cut(s) 295, 402, 552, 586, 702, 811, 834, 852
MboI GATC 8 cut(s) 293, 400, 550, 584, 700, 809, 832, 850
MboII GAAGA 2 cut(s) 819, 975
MfeI CAATTG 1 cut(s) 736
MflI RGATCY 4 cut(s) 400, 550, 584, 832
MhlI GDGCHC 2 cut(s) 255, 735
MluCI AATT 4 cut(s) 120, 689, 736, 1019
MlyI GAGTC 1 cut(s) 103
MmeI TCCRAC 2 cut(s) 169, 174
MnlI CCTC 8 cut(s) 343, 366, 391, 414, 613, 666, 733, 1003
MseI TTAA 2 cut(s) 408, 915
MspA1I CMGCKG 1 cut(s) 438
MspI CCGG 2 cut(s) 264, 713
MspR9I CCNGG 4 cut(s) 259, 264, 265, 607
MunI CAATTG 1 cut(s) 736
Mva1269I GAATGC 1 cut(s) 741
MvaI CCWGG 2 cut(s) 259, 607
MvnI CGCG 1 cut(s) 805
MwoI GCNNNNNNNGC 1 cut(s) 259
NciI CCSGG 2 cut(s) 264, 265
NdeII GATC 8 cut(s) 293, 400, 550, 584, 700, 809, 832, 850
NlaIII CATG 5 cut(s) 361, 392, 484, 617, 831
NlaIV GGNNCC 4 cut(s) 67, 402, 552, 872
NmuCI GTSAC 2 cut(s) 18, 283
NspI RCATGY 2 cut(s) 392, 617
PaeI GCATGC 1 cut(s) 617
PaeR7I CTCGAG 1 cut(s) 395
PceI AGGCCT 1 cut(s) 786
PcsI WCGNNNNNNNCGW 1 cut(s) 859
PctI GAATGC 1 cut(s) 741
PfeI GAWTC 1 cut(s) 310
PflFI GACNNNGTC 1 cut(s) 476
PkrI GCNGC 3 cut(s) 440, 980, 1036
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
PpuMI RGGWCCY 1 cut(s) 66
PshAI GACNNNNGTC 1 cut(s) 71
Psp5II RGGWCCY 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 257, 605
PspEI GGTNACC 1 cut(s) 283
PspGI CCWGG 2 cut(s) 257, 605
PspN4I GGNNCC 4 cut(s) 67, 402, 552, 872
PspPI GGNCC 4 cut(s) 66, 73, 150, 261
PspPPI RGGWCCY 1 cut(s) 66
PspXI VCTCGAGB 1 cut(s) 395
PsuI RGATCY 4 cut(s) 400, 550, 584, 832
PsyI GACNNNGTC 1 cut(s) 476
PvuII CAGCTG 1 cut(s) 438
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
SaqAI TTAA 2 cut(s) 408, 915
SatI GCNGC 3 cut(s) 439, 979, 1035
Sau3AI GATC 8 cut(s) 293, 400, 550, 584, 700, 809, 832, 850
Sau96I GGNCC 4 cut(s) 66, 73, 150, 261
SchI GAGTC 1 cut(s) 103
ScrFI CCNGG 4 cut(s) 259, 264, 265, 607
SduI GDGCHC 2 cut(s) 255, 735
SfaNI GCATC 2 cut(s) 450, 886
Sfr274I CTCGAG 1 cut(s) 395
SinI GGWCC 2 cut(s) 66, 73
SlaI CTCGAG 1 cut(s) 395
SmaI CCCGGG 1 cut(s) 265
SmlI CTYRAG 2 cut(s) 395, 777
SmoI CTYRAG 2 cut(s) 395, 777
SphI GCATGC 1 cut(s) 617
Sse9I AATT 4 cut(s) 120, 689, 736, 1019
SseBI AGGCCT 1 cut(s) 786
SsiI CCGC 2 cut(s) 446, 697
SspI AATATT 1 cut(s) 890
StuI AGGCCT 1 cut(s) 786
StyD4I CCNGG 4 cut(s) 257, 262, 263, 605
StyI CCWWGG 2 cut(s) 69, 726
TaaI ACNGT 4 cut(s) 24, 417, 455, 643
TaqI TCGA 4 cut(s) 242, 396, 933, 961
TasI AATT 4 cut(s) 120, 689, 736, 1019
TatI WGTACW 1 cut(s) 565
TfiI GAWTC 1 cut(s) 310
Tru1I TTAA 2 cut(s) 408, 915
Tru9I TTAA 2 cut(s) 408, 915
TscAI CASTG 4 cut(s) 596, 648, 819, 1068
TseFI GTSAC 2 cut(s) 18, 283
TseI GCWGC 3 cut(s) 438, 978, 1034
Tsp45I GTSAC 2 cut(s) 18, 283
TspDTI ATGAA 4 cut(s) 17, 149, 160, 963
TspGWI ACGGA 1 cut(s) 987
TspMI CCCGGG 1 cut(s) 263
TspRI CASTG 4 cut(s) 596, 648, 819, 1068
Tth111I GACNNNGTC 1 cut(s) 476
VneI GTGCAC 1 cut(s) 251
VpaK11BI GGWCC 2 cut(s) 66, 73
XapI RAATTY 2 cut(s) 689, 1019
XceI RCATGY 2 cut(s) 392, 617
XhoI CTCGAG 1 cut(s) 395
XmaI CCCGGG 1 cut(s) 263
XmiI GTMKAC 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.