Rroxscaffold_7G00191080

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002928
Physical Location & Seq
Forward (+)
31572615 .. 31583668
11054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00191080.1

Sequence Viewer

Length: 246 bp
ATGTCAACCAGAACCAGTTTTGTAGACAGCTTTGCTGATACAAAAGATGGCCGCTACAATCCAAAAATGGATCCTGTTATTCCTGTACAAAACTGGAGAAAAGGATCTCAGTGGGTTGTATTGACGAGGAAGCATGCAGAGGTTGTAGTGAAAGACGATACAGTGTTTCCTATGTTTCAGTTGTATTGTAAGAGGAAGTCGTTACCTGAGCTTTGGCGGGATCATCCTGTTATAAATTTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

81

Amino Acids

9.58

Weight (kDa)

9.66

Isoelectric Point (pI)

30.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 3 - 70 3.1e-17 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 233
AccI GTMKAC 1 cut(s) 24
AciI CCGC 2 cut(s) 52, 217
AclWI GGATC 4 cut(s) 65, 78, 112, 228
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 1 cut(s) 235
AfaI GTAC 1 cut(s) 87
AgsI TTSAA 1 cut(s) 241
AluBI AGCT 2 cut(s) 30, 211
AluI AGCT 2 cut(s) 30, 211
AlwI GGATC 4 cut(s) 65, 78, 112, 228
AoxI GGCC 1 cut(s) 49
ApoI RAATTY 1 cut(s) 235
BamHI GGATCC 1 cut(s) 70
BccI CCATC 1 cut(s) 41
BisI GCNGC 1 cut(s) 52
BlsI GCNGC 1 cut(s) 53
BmiI GGNNCC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 115
Bpu10I CCTNAGC 1 cut(s) 207
Bse1I ACTGG 2 cut(s) 15, 98
BseGI GGATG 1 cut(s) 223
BseMII CTCAG 2 cut(s) 122, 198
BseNI ACTGG 2 cut(s) 15, 98
BshFI GGCC 1 cut(s) 51
BsnI GGCC 1 cut(s) 51
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 3 cut(s) 70, 104, 220
BspACI CCGC 2 cut(s) 52, 217
BspANI GGCC 1 cut(s) 51
BspCNI CTCAG 2 cut(s) 121, 199
BspLI GGNNCC 1 cut(s) 72
BspPI GGATC 4 cut(s) 65, 78, 112, 228
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 2 cut(s) 15, 98
BssMI GATC 3 cut(s) 70, 104, 220
Bst4CI ACNGT 1 cut(s) 163
BstAUI TGTACA 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 2 cut(s) 108, 207
BstF5I GGATG 1 cut(s) 223
BstKTI GATC 3 cut(s) 73, 107, 223
BstMBI GATC 3 cut(s) 70, 104, 220
BstNSI RCATGY 1 cut(s) 137
BstX2I RGATCY 2 cut(s) 70, 104
BstYI RGATCY 2 cut(s) 70, 104
BsuRI GGCC 1 cut(s) 51
BtsCI GGATG 1 cut(s) 223
BtsIMutI CAGTG 2 cut(s) 116, 168
Cac8I GCNNGC 1 cut(s) 135
Csp6I GTAC 1 cut(s) 86
CviAII CATG 1 cut(s) 134
CviJI RGCY 3 cut(s) 30, 51, 211
CviKI_1 RGCY 3 cut(s) 30, 51, 211
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 2 cut(s) 108, 207
DpnI GATC 3 cut(s) 72, 106, 222
DpnII GATC 3 cut(s) 70, 104, 220
EaeI YGGCCR 1 cut(s) 49
FaeI CATG 1 cut(s) 137
FaiI YATR 3 cut(s) 135, 173, 233
FatI CATG 1 cut(s) 133
FauI CCCGC 1 cut(s) 210
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 1 cut(s) 52
FokI GGATG 1 cut(s) 210
Fsp4HI GCNGC 1 cut(s) 52
GluI GCNGC 1 cut(s) 52
GsuI CTGGAG 1 cut(s) 115
HaeIII GGCC 1 cut(s) 51
Hin1II CATG 1 cut(s) 137
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
Hpy166II GTNNAC 2 cut(s) 6, 25
Hpy8I GTNNAC 2 cut(s) 6, 25
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4V TGCA 1 cut(s) 137
HpyF3I CTNAG 2 cut(s) 108, 207
Hsp92II CATG 1 cut(s) 137
Kzo9I GATC 3 cut(s) 70, 104, 220
LpnPI CCDG 7 cut(s) 22, 28, 79, 87, 96, 219, 240
MaeIII GTNAC 1 cut(s) 201
MalI GATC 3 cut(s) 72, 106, 222
MboI GATC 3 cut(s) 70, 104, 220
MflI RGATCY 2 cut(s) 70, 104
MluCI AATT 1 cut(s) 235
MnlI CCTC 3 cut(s) 120, 133, 186
NdeII GATC 3 cut(s) 70, 104, 220
NlaIII CATG 1 cut(s) 137
NlaIV GGNNCC 1 cut(s) 72
NspI RCATGY 1 cut(s) 137
PaeI GCATGC 1 cut(s) 137
PkrI GCNGC 1 cut(s) 53
PsiI TTATAA 1 cut(s) 233
PspN4I GGNNCC 1 cut(s) 72
PsuI RGATCY 2 cut(s) 70, 104
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
SatI GCNGC 1 cut(s) 52
Sau3AI GATC 3 cut(s) 70, 104, 220
SetI ASST 4 cut(s) 32, 144, 208, 213
SphI GCATGC 1 cut(s) 137
Sse9I AATT 1 cut(s) 235
SsiI CCGC 2 cut(s) 52, 217
TaaI ACNGT 1 cut(s) 163
TasI AATT 1 cut(s) 235
TatI WGTACW 1 cut(s) 85
TauI GCSGC 1 cut(s) 54
TscAI CASTG 2 cut(s) 116, 168
TspRI CASTG 2 cut(s) 116, 168
XapI RAATTY 1 cut(s) 235
XceI RCATGY 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.