Rh2CG620700

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002928
Physical Location & Seq
Reverse (-)
79540676 .. 79545647
4972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG620700.1

Sequence Viewer

Length: 789 bp
ATGAAGCGAAAGTCACAGTTCAAATGGAAGCGGAAGCTTTTCGCTTCGATTTTGATGTGCTTGTGCTTTGGGACCTTGGTCCTTCTTATGCATACTCACTACACTCGGATTATGACTCTGGCCTCAATTCGTCCTCAGCTGACAATCCAACGGCCCAAGATTGCCTTCTTGTTCATCGCCCGCAACCGGTTACCGTTGGATATGCTCTGGGATGTTTTCTTTCAGGGCGGGGAGGGTAAGTTTTCGATTTATGTGCACTCCAGGCCCGGGTTTCTGTTCAATAGGGTCACCACCAGATCAGTTTTTTTCCTGAATCGTCAAGTCAATGATAGCATACAGGTAGATTGGGGAGAAGCAACCATGATTGAAGCGGAGCGTATATTGCTCAAACATGCACTAGAGGATCCTCTTAATCAACGCTTTGCTTTTGTTTCTGATAGCTGCATCCCTCTTTACAATTTCAACTATATATATGACTATATCATGTCAACCAGAACCAGTTTTGTAGACAGCTTTGCTGATACAAAAGATGGCCGCTACAATCCAAAAATGGATCCTGTTATTCCTGTACAAAACTGGAGAAAAGGATCTCAGTGGGTTGTATTGACGAGGAAGCATGCAGAGGTTGTAGTGAAAGACGATACAGTGTTTCCTATGTTTCAGTTGTATTGTAAGAGGAAGTCGTTACCTGAGTTTTGGCGGGATCATCCTGTTCCGGCCGATAACTCCAAGGAGCACAATTGCATACCAGACGAGCATTATGTTCAGACATTACTGGCTGTAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

262

Amino Acids

30.97

Weight (kDa)

9.51

Isoelectric Point (pI)

35.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 54 - 261 7.2e-63 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 507
AciI CCGC 6 cut(s) 31, 181, 228, 371, 535, 700
AclWI GGATC 6 cut(s) 398, 411, 548, 561, 595, 711
AcoI YGGCCR 2 cut(s) 532, 717
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 2 cut(s) 186, 267
AgeI ACCGGT 1 cut(s) 186
AgsI TTSAA 4 cut(s) 22, 280, 368, 463
AjnI CCWGG 1 cut(s) 260
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 4 cut(s) 37, 139, 441, 513
AluI AGCT 4 cut(s) 37, 139, 441, 513
Alw21I GWGCWC 2 cut(s) 258, 738
Alw44I GTGCAC 1 cut(s) 254
AlwI GGATC 6 cut(s) 398, 411, 548, 561, 595, 711
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 5 cut(s) 120, 152, 263, 532, 717
ApaLI GTGCAC 1 cut(s) 254
ApeKI GCWGC 1 cut(s) 441
AsiGI ACCGGT 1 cut(s) 186
Asp700I GAANNNNTTC 1 cut(s) 38
AspS9I GGNCC 4 cut(s) 72, 79, 153, 264
AsuC2I CCSGG 2 cut(s) 267, 268
AsuHPI GGTGA 1 cut(s) 280
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 2 cut(s) 72, 79
BaeGI GKGCMC 1 cut(s) 258
BamHI GGATCC 2 cut(s) 403, 553
Bbv12I GWGCWC 2 cut(s) 258, 738
BbvCI CCTCAGC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 428
BccI CCATC 1 cut(s) 524
BceAI ACGGC 1 cut(s) 167
BcgI CGANNNNNNTGC 2 cut(s) 235, 269
BciT130I CCWGG 1 cut(s) 262
BcnI CCSGG 2 cut(s) 267, 268
BfaI CTAG 1 cut(s) 398
BisI GCNGC 2 cut(s) 442, 535
BlsI GCNGC 2 cut(s) 443, 536
Bme1390I CCNGG 3 cut(s) 262, 267, 268
Bme18I GGWCC 2 cut(s) 72, 79
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 4 cut(s) 72, 79, 153, 264
BmiI GGNNCC 3 cut(s) 73, 405, 555
BmrFI CCNGG 3 cut(s) 262, 267, 268
BmsI GCATC 1 cut(s) 453
BoxI GACNNNNGTC 1 cut(s) 77
BpmI CTGGAG 2 cut(s) 244, 598
Bpu10I CCTNAGC 1 cut(s) 135
BpuMI CCSGG 2 cut(s) 267, 268
BsaJI CCNNGG 3 cut(s) 75, 266, 729
BsaWI WCCGGW 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 267
Bse118I RCCGGY 1 cut(s) 186
Bse1I ACTGG 3 cut(s) 498, 581, 780
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 3 cut(s) 75, 266, 729
BseGI GGATG 3 cut(s) 217, 444, 706
BseLI CCNNNNNNNGG 2 cut(s) 186, 267
BseMII CTCAG 3 cut(s) 149, 605, 681
BseNI ACTGG 3 cut(s) 498, 581, 780
BseSI GKGCMC 1 cut(s) 258
BseX3I CGGCCG 1 cut(s) 717
BseXI GCAGC 1 cut(s) 428
Bsh1285I CGRYCG 1 cut(s) 720
BshFI GGCC 5 cut(s) 122, 154, 265, 534, 719
BshTI ACCGGT 1 cut(s) 186
BsiEI CGRYCG 1 cut(s) 720
BsiHKAI GWGCWC 2 cut(s) 258, 738
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 3 cut(s) 187, 267, 716
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 2 cut(s) 186, 267
BsmFI GGGAC 1 cut(s) 85
BsnI GGCC 5 cut(s) 122, 154, 265, 534, 719
BsoBI CYCGRG 1 cut(s) 266
Bsp1286I GDGCHC 2 cut(s) 258, 738
Bsp1407I TGTACA 1 cut(s) 568
Bsp143I GATC 5 cut(s) 296, 403, 553, 587, 703
BspACI CCGC 6 cut(s) 31, 181, 228, 371, 535, 700
BspANI GGCC 5 cut(s) 122, 154, 265, 534, 719
BspCNI CTCAG 3 cut(s) 148, 604, 682
BspLI GGNNCC 3 cut(s) 73, 405, 555
BspPI GGATC 6 cut(s) 398, 411, 548, 561, 595, 711
BsrFI RCCGGY 1 cut(s) 186
BsrGI TGTACA 1 cut(s) 568
BsrI ACTGG 3 cut(s) 498, 581, 780
BssAI RCCGGY 1 cut(s) 186
BssECI CCNNGG 3 cut(s) 75, 266, 729
BssMI GATC 5 cut(s) 296, 403, 553, 587, 703
BssT1I CCWWGG 2 cut(s) 75, 729
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 3 cut(s) 18, 195, 646
BstAUI TGTACA 1 cut(s) 568
BstC8I GCNNGC 2 cut(s) 181, 618
BstDEI CTNAG 3 cut(s) 135, 591, 690
BstEII GGTNACC 2 cut(s) 189, 286
BstF5I GGATG 3 cut(s) 217, 444, 706
BstKTI GATC 5 cut(s) 299, 406, 556, 590, 706
BstMBI GATC 5 cut(s) 296, 403, 553, 587, 703
BstMCI CGRYCG 1 cut(s) 720
BstMWI GCNNNNNNNGC 2 cut(s) 262, 382
BstNI CCWGG 1 cut(s) 262
BstNSI RCATGY 2 cut(s) 395, 620
BstPAI GACNNNNGTC 1 cut(s) 77
BstPI GGTNACC 2 cut(s) 189, 286
BstSCI CCNGG 3 cut(s) 260, 265, 266
BstSLI GKGCMC 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 428
BstX2I RGATCY 3 cut(s) 403, 553, 587
BstYI RGATCY 3 cut(s) 403, 553, 587
BstZI CGGCCG 1 cut(s) 717
BsuRI GGCC 5 cut(s) 122, 154, 265, 534, 719
BtgZI GCGATG 1 cut(s) 160
BtsCI GGATG 3 cut(s) 217, 444, 706
BtsIMutI CAGTG 2 cut(s) 599, 651
Cac8I GCNNGC 2 cut(s) 181, 618
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 4 cut(s) 72, 79, 153, 264
Cfr9I CCCGGG 1 cut(s) 266
Csp6I GTAC 1 cut(s) 569
CspAI ACCGGT 1 cut(s) 186
CviAII CATG 4 cut(s) 361, 392, 484, 617
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 3 cut(s) 135, 591, 690
DpnI GATC 5 cut(s) 298, 405, 555, 589, 705
DpnII GATC 5 cut(s) 296, 403, 553, 587, 703
EaeI YGGCCR 2 cut(s) 532, 717
EagI CGGCCG 1 cut(s) 717
EclXI CGGCCG 1 cut(s) 717
Eco130I CCWWGG 2 cut(s) 75, 729
Eco47I GGWCC 2 cut(s) 72, 79
Eco52I CGGCCG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 266
Eco91I GGTNACC 2 cut(s) 189, 286
EcoO109I RGGNCCY 1 cut(s) 72
EcoO65I GGTNACC 2 cut(s) 189, 286
EcoRII CCWGG 1 cut(s) 260
EcoT14I CCWWGG 2 cut(s) 75, 729
EcoT22I ATGCAT 1 cut(s) 93
ErhI CCWWGG 2 cut(s) 75, 729
FaeI CATG 4 cut(s) 364, 395, 487, 620
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FaqI GGGAC 1 cut(s) 85
FatI CATG 4 cut(s) 360, 391, 483, 616
FauI CCCGC 3 cut(s) 188, 221, 693
FblI GTMKAC 1 cut(s) 507
Fnu4HI GCNGC 2 cut(s) 442, 535
FokI GGATG 3 cut(s) 224, 431, 693
Fsp4HI GCNGC 2 cut(s) 442, 535
FspBI CTAG 1 cut(s) 398
GluI GCNGC 2 cut(s) 442, 535
GsuI CTGGAG 2 cut(s) 244, 598
HaeIII GGCC 5 cut(s) 122, 154, 265, 534, 719
HapII CCGG 3 cut(s) 187, 267, 716
Hin1II CATG 4 cut(s) 364, 395, 487, 620
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 2 cut(s) 115, 313
HpaII CCGG 3 cut(s) 187, 267, 716
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 3 cut(s) 256, 489, 508
Hpy188I TCNGA 3 cut(s) 108, 436, 768
Hpy188III TCNNGA 1 cut(s) 310
Hpy8I GTNNAC 3 cut(s) 256, 489, 508
HpyAV CCTTC 2 cut(s) 92, 175
HpyCH4III ACNGT 3 cut(s) 18, 195, 646
HpyCH4V TGCA 6 cut(s) 91, 256, 395, 444, 620, 744
HpyF10VI GCNNNNNNNGC 2 cut(s) 262, 382
HpyF3I CTNAG 3 cut(s) 135, 591, 690
Hsp92II CATG 4 cut(s) 364, 395, 487, 620
Kzo9I GATC 5 cut(s) 296, 403, 553, 587, 703
LmnI GCTCC 2 cut(s) 373, 733
Lsp1109I GCAGC 1 cut(s) 428
LweI GCATC 1 cut(s) 453
MaeI CTAG 1 cut(s) 398
MaeIII GTNAC 4 cut(s) 12, 189, 286, 684
MalI GATC 5 cut(s) 298, 405, 555, 589, 705
MboI GATC 5 cut(s) 296, 403, 553, 587, 703
MfeI CAATTG 1 cut(s) 739
MflI RGATCY 3 cut(s) 403, 553, 587
MhlI GDGCHC 2 cut(s) 258, 738
MluCI AATT 3 cut(s) 126, 457, 739
MlyI GAGTC 1 cut(s) 109
MmeI TCCRAC 2 cut(s) 172, 177
MnlI CCTC 9 cut(s) 133, 144, 226, 394, 417, 459, 603, 616, 669
Mph1103I ATGCAT 1 cut(s) 93
MroXI GAANNNNTTC 1 cut(s) 38
MseI TTAA 1 cut(s) 411
MspA1I CMGCKG 1 cut(s) 139
MspI CCGG 3 cut(s) 187, 267, 716
MspR9I CCNGG 3 cut(s) 262, 267, 268
MunI CAATTG 1 cut(s) 739
MvaI CCWGG 1 cut(s) 262
MwoI GCNNNNNNNGC 2 cut(s) 262, 382
NciI CCSGG 2 cut(s) 267, 268
NdeII GATC 5 cut(s) 296, 403, 553, 587, 703
NlaIII CATG 4 cut(s) 364, 395, 487, 620
NlaIV GGNNCC 3 cut(s) 73, 405, 555
NmuCI GTSAC 2 cut(s) 12, 286
NsiI ATGCAT 1 cut(s) 93
NspI RCATGY 2 cut(s) 395, 620
PaeI GCATGC 1 cut(s) 620
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 1 cut(s) 313
PinAI ACCGGT 1 cut(s) 186
PkrI GCNGC 2 cut(s) 443, 536
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PpuMI RGGWCCY 1 cut(s) 72
PshAI GACNNNNGTC 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 260
PspEI GGTNACC 2 cut(s) 189, 286
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 3 cut(s) 73, 405, 555
PspPI GGNCC 4 cut(s) 72, 79, 153, 264
PspPPI RGGWCCY 1 cut(s) 72
PsuI RGATCY 3 cut(s) 403, 553, 587
PvuII CAGCTG 1 cut(s) 139
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 2 cut(s) 442, 535
Sau3AI GATC 5 cut(s) 296, 403, 553, 587, 703
Sau96I GGNCC 4 cut(s) 72, 79, 153, 264
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 3 cut(s) 262, 267, 268
SduI GDGCHC 2 cut(s) 258, 738
SetI ASST 8 cut(s) 39, 77, 141, 342, 443, 515, 627, 691
SfaNI GCATC 1 cut(s) 453
SinI GGWCC 2 cut(s) 72, 79
SmaI CCCGGG 1 cut(s) 268
SphI GCATGC 1 cut(s) 620
Sse9I AATT 3 cut(s) 126, 457, 739
SsiI CCGC 6 cut(s) 31, 181, 228, 371, 535, 700
SspMI CTAG 1 cut(s) 398
StyD4I CCNGG 3 cut(s) 260, 265, 266
StyI CCWWGG 2 cut(s) 75, 729
TaaI ACNGT 3 cut(s) 18, 195, 646
TaqI TCGA 2 cut(s) 47, 245
TasI AATT 3 cut(s) 126, 457, 739
TatI WGTACW 1 cut(s) 568
TauI GCSGC 1 cut(s) 537
TfiI GAWTC 1 cut(s) 313
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 2 cut(s) 599, 651
TseFI GTSAC 2 cut(s) 12, 286
TseI GCWGC 1 cut(s) 441
Tsp45I GTSAC 2 cut(s) 12, 286
TspDTI ATGAA 2 cut(s) 17, 163
TspMI CCCGGG 1 cut(s) 266
TspRI CASTG 2 cut(s) 599, 651
VneI GTGCAC 1 cut(s) 254
VpaK11BI GGWCC 2 cut(s) 72, 79
XceI RCATGY 2 cut(s) 395, 620
XmaI CCCGGG 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 507
XmnI GAANNNNTTC 1 cut(s) 38
XspI CTAG 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.