RLG00000022155

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002928
Physical Location & Seq
Reverse (-)
81399780 .. 81403832
4053 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022155

Sequence Viewer

Length: 1080 bp
ATGAAGCGAAAGTCACAGTTCAAATGGAAGCGGAAGCTTTTCGCTTCGATTTTGATGTGCTTGTGCTTTGGGACCTTGGTCCTTCTTATGCATACTCACTACACTCGGATTATGACCCTGGCTTCAATTCGTCCTCAGATGACAATCCAACGGCCCAAGATTGCCTTCTTGTTCATCGCCCGCAACCGGTTACCGTTGGATATGCTCTGGGATGTTTTCTTTCAGGGCGGGGAGGGTAAGTTTTCTATTTATGTGCACTCCAGGCCCGGGTTTCTGTTCAATAAGGTCACCACCAGATCAGGTTTTTTCCTGAATCGTCAAGTCAATGATAGCATACAGGTAGATTGGGGAGAAGCAACCATGATTGAAGCGGAGCGTATATTGCTCAAACATGCACTAGAGGATCCTCTTAATCAACGCTTTGCTTTTGTTTCTGATAGTTGCATCCCTCTTTACAATTTCAACTATATATATGACTATATCATGTCAACCAGAACCAGTTTTGTAGACAGCTTTGCTGATACAAAAGATGGCCGCTACAATCCAAAAATGGATCCTGTTATTCCTGTACAAAACTGGAGAAAAGGATCTCAGTGGGTTGTATTGACGAGGAAGCATGCAGAGGTTGTAGTGAAAGATGATACAGTGTTTCCTATGTTTCAGTTGTATTGCAAGAGGAAGTCGTTACCTGAGTTTTGGCGGGATCGTCCTGTTCCAGCCGATAACTCCAAGGAGCACAATTGCATTCCGGACGAGCATTATGTTCAGACATTACTGGCTCAAGAGGGCCTTGAAGAAGAAATCACACGAAGATCACTGACACACTCATCATGGGATTTATCATCCACTGGAGACCGTGAACGCCGTGGATGGCACCCTATGACATACAAATATTCAGATGCTACTCCCATGCTTATTAAATCTATAAAGGATGTAGATAATATCTACTATGAGACCGAGTATCGAAGAGAATGGTGTAGCAGCAAGGAGAGACCCTCTCCATGCTTTCTTTTTGCCAGAAAATTTACCCGGCCTGCTGCTCTCAGGCTTCTCAACACGACTACACTGGAGCTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

360

Amino Acids

42.48

Weight (kDa)

9.38

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 54 - 309 4.8e-74 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 873
AccI GTMKAC 1 cut(s) 507
AccIII TCCGGA 1 cut(s) 748
AciI CCGC 6 cut(s) 31, 181, 228, 371, 535, 700
AclWI GGATC 6 cut(s) 398, 411, 548, 561, 595, 711
AcoI YGGCCR 1 cut(s) 532
AcsI RAATTY 1 cut(s) 1022
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 2 cut(s) 186, 267
AgeI ACCGGT 1 cut(s) 186
AgsI TTSAA 6 cut(s) 22, 126, 280, 368, 463, 794
AjnI CCWGG 2 cut(s) 117, 260
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 3 cut(s) 37, 513, 1072
AluI AGCT 3 cut(s) 37, 513, 1072
Alw21I GWGCWC 2 cut(s) 258, 738
Alw26I GTCTC 3 cut(s) 846, 947, 985
Alw44I GTGCAC 1 cut(s) 254
AlwI GGATC 6 cut(s) 398, 411, 548, 561, 595, 711
Ama87I CYCGRG 1 cut(s) 266
Aor13HI TCCGGA 1 cut(s) 748
AoxI GGCC 5 cut(s) 152, 263, 532, 787, 1031
ApaLI GTGCAC 1 cut(s) 254
ApeKI GCWGC 2 cut(s) 981, 1037
ApoI RAATTY 1 cut(s) 1022
AsiGI ACCGGT 1 cut(s) 186
Asp700I GAANNNNTTC 1 cut(s) 38
AspS9I GGNCC 5 cut(s) 72, 79, 153, 264, 787
AsuC2I CCSGG 3 cut(s) 267, 268, 1030
AsuHPI GGTGA 1 cut(s) 280
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 2 cut(s) 72, 79
BaeGI GKGCMC 1 cut(s) 258
BamHI GGATCC 2 cut(s) 403, 553
BanI GGYRCC 1 cut(s) 873
Bbv12I GWGCWC 2 cut(s) 258, 738
BbvI GCAGC 2 cut(s) 993, 1024
BccI CCATC 2 cut(s) 524, 864
BceAI ACGGC 2 cut(s) 167, 849
BciT130I CCWGG 2 cut(s) 119, 262
BcnI CCSGG 3 cut(s) 267, 268, 1030
BcoDI GTCTC 3 cut(s) 846, 947, 985
BfaI CTAG 1 cut(s) 398
BisI GCNGC 3 cut(s) 535, 982, 1038
BlsI GCNGC 3 cut(s) 536, 983, 1039
Bme1390I CCNGG 5 cut(s) 119, 262, 267, 268, 1030
Bme18I GGWCC 2 cut(s) 72, 79
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 5 cut(s) 72, 79, 153, 264, 787
BmiI GGNNCC 4 cut(s) 73, 405, 555, 875
BmrFI CCNGG 5 cut(s) 119, 262, 267, 268, 1030
BmsI GCATC 2 cut(s) 453, 889
BoxI GACNNNNGTC 1 cut(s) 77
BplI GAGNNNNNCTC 4 cut(s) 980, 1012, 982, 1014
BpmI CTGGAG 3 cut(s) 244, 598, 870
BpuEI CTTGAG 1 cut(s) 765
BpuMI CCSGG 3 cut(s) 267, 268, 1030
BsaBI GATNNNNATC 1 cut(s) 143
BsaI GGTCTC 3 cut(s) 846, 947, 985
BsaJI CCNNGG 5 cut(s) 75, 117, 266, 729, 865
BsaWI WCCGGW 2 cut(s) 186, 748
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 267
Bse118I RCCGGY 1 cut(s) 186
Bse1I ACTGG 5 cut(s) 498, 581, 780, 853, 1071
Bse8I GATNNNNATC 1 cut(s) 143
BseAI TCCGGA 1 cut(s) 748
BseBI CCWGG 2 cut(s) 119, 262
BseDI CCNNGG 5 cut(s) 75, 117, 266, 729, 865
BseGI GGATG 5 cut(s) 217, 444, 842, 875, 937
BseJI GATNNNNATC 1 cut(s) 143
BseLI CCNNNNNNNGG 2 cut(s) 186, 267
BseMII CTCAG 4 cut(s) 149, 605, 681, 1057
BseNI ACTGG 5 cut(s) 498, 581, 780, 853, 1071
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 2 cut(s) 993, 1024
BshFI GGCC 5 cut(s) 154, 265, 534, 789, 1033
BshNI GGYRCC 1 cut(s) 873
BshTI ACCGGT 1 cut(s) 186
BsiHKAI GWGCWC 2 cut(s) 258, 738
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 4 cut(s) 187, 267, 749, 1030
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 2 cut(s) 186, 267
BsmAI GTCTC 3 cut(s) 846, 947, 985
BsmFI GGGAC 1 cut(s) 85
BsmI GAATGC 1 cut(s) 744
BsnI GGCC 5 cut(s) 154, 265, 534, 789, 1033
Bso31I GGTCTC 3 cut(s) 846, 947, 985
BsoBI CYCGRG 1 cut(s) 266
Bsp1286I GDGCHC 2 cut(s) 258, 738
Bsp13I TCCGGA 1 cut(s) 748
Bsp1407I TGTACA 1 cut(s) 568
Bsp143I GATC 6 cut(s) 296, 403, 553, 587, 703, 812
BspACI CCGC 6 cut(s) 31, 181, 228, 371, 535, 700
BspANI GGCC 5 cut(s) 154, 265, 534, 789, 1033
BspCNI CTCAG 4 cut(s) 148, 604, 682, 1056
BspEI TCCGGA 1 cut(s) 748
BspLI GGNNCC 4 cut(s) 73, 405, 555, 875
BspPI GGATC 6 cut(s) 398, 411, 548, 561, 595, 711
BspT107I GGYRCC 1 cut(s) 873
BspTNI GGTCTC 3 cut(s) 846, 947, 985
BsrFI RCCGGY 1 cut(s) 186
BsrGI TGTACA 1 cut(s) 568
BsrI ACTGG 5 cut(s) 498, 581, 780, 853, 1071
BssAI RCCGGY 1 cut(s) 186
BssECI CCNNGG 5 cut(s) 75, 117, 266, 729, 865
BssMI GATC 6 cut(s) 296, 403, 553, 587, 703, 812
BssT1I CCWWGG 2 cut(s) 75, 729
Bst2UI CCWGG 2 cut(s) 119, 262
Bst4CI ACNGT 4 cut(s) 18, 195, 646, 857
Bst6I CTCTTC 1 cut(s) 961
BstAUI TGTACA 1 cut(s) 568
BstC8I GCNNGC 3 cut(s) 181, 618, 1035
BstDEI CTNAG 5 cut(s) 135, 591, 690, 1043, 1073
BstDSI CCRYGG 1 cut(s) 865
BstEII GGTNACC 2 cut(s) 189, 286
BstF5I GGATG 5 cut(s) 217, 444, 842, 875, 937
BstKTI GATC 6 cut(s) 299, 406, 556, 590, 706, 815
BstMAI GTCTC 3 cut(s) 846, 947, 985
BstMBI GATC 6 cut(s) 296, 403, 553, 587, 703, 812
BstMWI GCNNNNNNNGC 2 cut(s) 262, 382
BstNI CCWGG 2 cut(s) 119, 262
BstNSI RCATGY 2 cut(s) 395, 620
BstPAI GACNNNNGTC 1 cut(s) 77
BstPI GGTNACC 2 cut(s) 189, 286
BstSCI CCNGG 5 cut(s) 117, 260, 265, 266, 1028
BstSLI GKGCMC 1 cut(s) 258
BstV1I GCAGC 2 cut(s) 993, 1024
BstX2I RGATCY 3 cut(s) 403, 553, 587
BstYI RGATCY 3 cut(s) 403, 553, 587
BsuRI GGCC 5 cut(s) 154, 265, 534, 789, 1033
BtgI CCRYGG 1 cut(s) 865
BtgZI GCGATG 1 cut(s) 160
BtsCI GGATG 5 cut(s) 217, 444, 842, 875, 937
BtsIMutI CAGTG 5 cut(s) 599, 651, 815, 846, 1064
Cac8I GCNNGC 3 cut(s) 181, 618, 1035
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 5 cut(s) 72, 79, 153, 264, 787
Cfr9I CCCGGG 1 cut(s) 266
Csp6I GTAC 1 cut(s) 569
CspAI ACCGGT 1 cut(s) 186
CviAII CATG 7 cut(s) 361, 392, 484, 617, 831, 910, 1002
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 5 cut(s) 135, 591, 690, 1043, 1073
DpnI GATC 6 cut(s) 298, 405, 555, 589, 705, 814
DpnII GATC 6 cut(s) 296, 403, 553, 587, 703, 812
EaeI YGGCCR 1 cut(s) 532
Eam1104I CTCTTC 1 cut(s) 961
EarI CTCTTC 1 cut(s) 961
Eco130I CCWWGG 2 cut(s) 75, 729
Eco31I GGTCTC 3 cut(s) 846, 947, 985
Eco47I GGWCC 2 cut(s) 72, 79
Eco88I CYCGRG 1 cut(s) 266
Eco91I GGTNACC 2 cut(s) 189, 286
EcoO109I RGGNCCY 2 cut(s) 72, 787
EcoO65I GGTNACC 2 cut(s) 189, 286
EcoRII CCWGG 2 cut(s) 117, 260
EcoT14I CCWWGG 2 cut(s) 75, 729
EcoT22I ATGCAT 1 cut(s) 93
ErhI CCWWGG 2 cut(s) 75, 729
FaeI CATG 7 cut(s) 364, 395, 487, 620, 834, 913, 1005
FalI AAGNNNNNCTT 4 cut(s) 149, 181, 774, 806
FaqI GGGAC 1 cut(s) 85
FatI CATG 7 cut(s) 360, 391, 483, 616, 830, 909, 1001
FauI CCCGC 3 cut(s) 188, 221, 693
FblI GTMKAC 1 cut(s) 507
Fnu4HI GCNGC 3 cut(s) 535, 982, 1038
FokI GGATG 5 cut(s) 224, 431, 829, 882, 944
Fsp4HI GCNGC 3 cut(s) 535, 982, 1038
FspBI CTAG 1 cut(s) 398
GluI GCNGC 3 cut(s) 535, 982, 1038
GsuI CTGGAG 3 cut(s) 244, 598, 870
HaeIII GGCC 5 cut(s) 154, 265, 534, 789, 1033
HapII CCGG 4 cut(s) 187, 267, 749, 1030
Hin1II CATG 7 cut(s) 364, 395, 487, 620, 834, 913, 1005
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 1 cut(s) 313
HpaII CCGG 4 cut(s) 187, 267, 749, 1030
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 4 cut(s) 256, 489, 508, 860
Hpy188I TCNGA 5 cut(s) 108, 138, 436, 768, 898
Hpy188III TCNNGA 3 cut(s) 310, 749, 782
Hpy8I GTNNAC 4 cut(s) 256, 489, 508, 860
HpyAV CCTTC 2 cut(s) 92, 175
HpyCH4III ACNGT 4 cut(s) 18, 195, 646, 857
HpyCH4V TGCA 7 cut(s) 91, 256, 395, 444, 620, 672, 744
HpyF10VI GCNNNNNNNGC 2 cut(s) 262, 382
HpyF3I CTNAG 5 cut(s) 135, 591, 690, 1043, 1073
Hsp92II CATG 7 cut(s) 364, 395, 487, 620, 834, 913, 1005
Kpn2I TCCGGA 1 cut(s) 748
Kzo9I GATC 6 cut(s) 296, 403, 553, 587, 703, 812
LmnI GCTCC 3 cut(s) 373, 733, 1069
Lsp1109I GCAGC 2 cut(s) 993, 1024
LweI GCATC 2 cut(s) 453, 889
MaeI CTAG 1 cut(s) 398
MaeIII GTNAC 4 cut(s) 12, 189, 286, 684
MalI GATC 6 cut(s) 298, 405, 555, 589, 705, 814
MboI GATC 6 cut(s) 296, 403, 553, 587, 703, 812
MboII GAAGA 4 cut(s) 806, 809, 822, 978
MfeI CAATTG 1 cut(s) 739
MflI RGATCY 3 cut(s) 403, 553, 587
MhlI GDGCHC 2 cut(s) 258, 738
MluCI AATT 4 cut(s) 126, 457, 739, 1022
MmeI TCCRAC 2 cut(s) 172, 177
Mph1103I ATGCAT 1 cut(s) 93
MroI TCCGGA 1 cut(s) 748
MroXI GAANNNNTTC 1 cut(s) 38
MseI TTAA 2 cut(s) 411, 918
MspI CCGG 4 cut(s) 187, 267, 749, 1030
MspR9I CCNGG 5 cut(s) 119, 262, 267, 268, 1030
MunI CAATTG 1 cut(s) 739
Mva1269I GAATGC 1 cut(s) 744
MvaI CCWGG 2 cut(s) 119, 262
MwoI GCNNNNNNNGC 2 cut(s) 262, 382
NciI CCSGG 3 cut(s) 267, 268, 1030
NdeII GATC 6 cut(s) 296, 403, 553, 587, 703, 812
NlaIII CATG 7 cut(s) 364, 395, 487, 620, 834, 913, 1005
NlaIV GGNNCC 4 cut(s) 73, 405, 555, 875
NmuCI GTSAC 2 cut(s) 12, 286
NsiI ATGCAT 1 cut(s) 93
NspI RCATGY 2 cut(s) 395, 620
PaeI GCATGC 1 cut(s) 620
PctI GAATGC 1 cut(s) 744
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 1 cut(s) 313
PinAI ACCGGT 1 cut(s) 186
PkrI GCNGC 3 cut(s) 536, 983, 1039
PpuMI RGGWCCY 1 cut(s) 72
PshAI GACNNNNGTC 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 72
Psp6I CCWGG 2 cut(s) 117, 260
PspEI GGTNACC 2 cut(s) 189, 286
PspGI CCWGG 2 cut(s) 117, 260
PspN4I GGNNCC 4 cut(s) 73, 405, 555, 875
PspPI GGNCC 5 cut(s) 72, 79, 153, 264, 787
PspPPI RGGWCCY 1 cut(s) 72
PsuI RGATCY 3 cut(s) 403, 553, 587
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
SaqAI TTAA 2 cut(s) 411, 918
SatI GCNGC 3 cut(s) 535, 982, 1038
Sau3AI GATC 6 cut(s) 296, 403, 553, 587, 703, 812
Sau96I GGNCC 5 cut(s) 72, 79, 153, 264, 787
ScrFI CCNGG 5 cut(s) 119, 262, 267, 268, 1030
SduI GDGCHC 2 cut(s) 258, 738
SetI ASST 9 cut(s) 39, 77, 288, 304, 342, 515, 627, 691, 1074
SfaNI GCATC 2 cut(s) 453, 889
SinI GGWCC 2 cut(s) 72, 79
SmaI CCCGGG 1 cut(s) 268
SmlI CTYRAG 1 cut(s) 780
SmoI CTYRAG 1 cut(s) 780
SphI GCATGC 1 cut(s) 620
Sse9I AATT 4 cut(s) 126, 457, 739, 1022
SsiI CCGC 6 cut(s) 31, 181, 228, 371, 535, 700
SspI AATATT 1 cut(s) 893
SspMI CTAG 1 cut(s) 398
StyD4I CCNGG 5 cut(s) 117, 260, 265, 266, 1028
StyI CCWWGG 2 cut(s) 75, 729
TaaI ACNGT 4 cut(s) 18, 195, 646, 857
TaqI TCGA 2 cut(s) 47, 964
TaqII GACCGA 1 cut(s) 971
TasI AATT 4 cut(s) 126, 457, 739, 1022
TatI WGTACW 1 cut(s) 568
TauI GCSGC 1 cut(s) 537
TfiI GAWTC 1 cut(s) 313
Tru1I TTAA 2 cut(s) 411, 918
Tru9I TTAA 2 cut(s) 411, 918
TscAI CASTG 5 cut(s) 599, 651, 822, 853, 1071
TseFI GTSAC 2 cut(s) 12, 286
TseI GCWGC 2 cut(s) 981, 1037
Tsp45I GTSAC 2 cut(s) 12, 286
TspDTI ATGAA 2 cut(s) 17, 163
TspMI CCCGGG 1 cut(s) 266
TspRI CASTG 5 cut(s) 599, 651, 822, 853, 1071
VneI GTGCAC 1 cut(s) 254
VpaK11BI GGWCC 2 cut(s) 72, 79
XapI RAATTY 1 cut(s) 1022
XceI RCATGY 2 cut(s) 395, 620
XmaI CCCGGG 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 507
XmnI GAANNNNTTC 1 cut(s) 38
XspI CTAG 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.