Rh5AG303700

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002928
Physical Location & Seq
Reverse (-)
43061049 .. 43065198
4150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG303700.1

Sequence Viewer

Length: 585 bp
ATGTCAACCATAACCAGTTTTGTAGACAGCTTTGCTGATACAAAAGATGGCCGCTACAATCCAAAAATGGATCCTGTTATTCCTGTACAAAACTGGAGAAAAGGATCTCAGTGGGTTGTATTGACGAGGAAGCATGCAGAGGTTGTAGTGAAAGACGATACAGTGTTTCCTATGTTTCAGTTGTATTGTAAGAGGAAGTCGTTACCTGAGTTTTGGCGGGATCATCCTGTTCCGGCCGATAACTCTAAGGAGCACAATTGCATTCCAGACGAGCATTATGTTCAGACATTACTGGTTCAAGAAGGCCTTGAAGGAGAAATCACACGAAGATCACTGACACACTCATCATGGGATTTATCATCCACTGCAGACCGTGAACGCCGTGGATGGCACCCTATGACATACAAATATTCAGATGCTACTCCCATGCTTATTAAATCTATAAAGGATGTAGATAATATCTACTATGAGACCGAGGGAGATTGTGCAAATTTGGTCTCTTTCTTTGAGGGGTCAGGTCACACAAACAAGAAGAAGAAGAAGAAGAAGCACAAGAAAGTTCTCCTGAGGCATGGCTGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

194

Amino Acids

22.57

Weight (kDa)

8.94

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 5 - 148 6.8e-33 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 390
AccI GTMKAC 1 cut(s) 24
AciI CCGC 2 cut(s) 52, 217
AclWI GGATC 4 cut(s) 65, 78, 112, 228
AcoI YGGCCR 2 cut(s) 49, 234
AcsI RAATTY 1 cut(s) 490
AfaI GTAC 1 cut(s) 87
AgsI TTSAA 2 cut(s) 299, 311
AluBI AGCT 1 cut(s) 30
AluI AGCT 1 cut(s) 30
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 2 cut(s) 464, 502
AlwI GGATC 4 cut(s) 65, 78, 112, 228
AoxI GGCC 3 cut(s) 49, 234, 304
ApeKI GCWGC 1 cut(s) 576
ApoI RAATTY 1 cut(s) 490
AxyI CCTNAGG 1 cut(s) 566
BamHI GGATCC 1 cut(s) 70
BanI GGYRCC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 563
BccI CCATC 2 cut(s) 41, 381
BceAI ACGGC 1 cut(s) 366
BcoDI GTCTC 2 cut(s) 464, 502
BfmI CTRYAG 1 cut(s) 366
BisI GCNGC 2 cut(s) 52, 577
BlsI GCNGC 2 cut(s) 53, 578
BmiI GGNNCC 2 cut(s) 72, 392
BmsI GCATC 1 cut(s) 406
BpmI CTGGAG 1 cut(s) 115
BsaI GGTCTC 2 cut(s) 464, 502
BsaJI CCNNGG 2 cut(s) 382, 474
Bse1I ACTGG 3 cut(s) 15, 98, 297
Bse21I CCTNAGG 1 cut(s) 566
BseDI CCNNGG 2 cut(s) 382, 474
BseGI GGATG 4 cut(s) 223, 359, 392, 454
BseMII CTCAG 3 cut(s) 122, 198, 557
BseNI ACTGG 3 cut(s) 15, 98, 297
BseX3I CGGCCG 1 cut(s) 234
BseXI GCAGC 1 cut(s) 563
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 3 cut(s) 51, 236, 306
BshNI GGYRCC 1 cut(s) 390
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 1 cut(s) 233
BsmAI GTCTC 2 cut(s) 464, 502
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 3 cut(s) 51, 236, 306
Bso31I GGTCTC 2 cut(s) 464, 502
Bsp1286I GDGCHC 1 cut(s) 255
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 4 cut(s) 70, 104, 220, 329
BspACI CCGC 2 cut(s) 52, 217
BspANI GGCC 3 cut(s) 51, 236, 306
BspCNI CTCAG 3 cut(s) 121, 199, 558
BspLI GGNNCC 2 cut(s) 72, 392
BspMAI CTGCAG 1 cut(s) 370
BspPI GGATC 4 cut(s) 65, 78, 112, 228
BspT107I GGYRCC 1 cut(s) 390
BspTNI GGTCTC 2 cut(s) 464, 502
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 3 cut(s) 15, 98, 297
BssECI CCNNGG 2 cut(s) 382, 474
BssMI GATC 4 cut(s) 70, 104, 220, 329
Bst4CI ACNGT 2 cut(s) 163, 374
BstAUI TGTACA 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 4 cut(s) 108, 207, 246, 566
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 4 cut(s) 223, 359, 392, 454
BstKTI GATC 4 cut(s) 73, 107, 223, 332
BstMAI GTCTC 2 cut(s) 464, 502
BstMBI GATC 4 cut(s) 70, 104, 220, 329
BstMCI CGRYCG 1 cut(s) 237
BstNSI RCATGY 1 cut(s) 137
BstSFI CTRYAG 1 cut(s) 366
BstV1I GCAGC 1 cut(s) 563
BstX2I RGATCY 2 cut(s) 70, 104
BstYI RGATCY 2 cut(s) 70, 104
BstZI CGGCCG 1 cut(s) 234
Bsu36I CCTNAGG 1 cut(s) 566
BsuRI GGCC 3 cut(s) 51, 236, 306
BtgI CCRYGG 1 cut(s) 382
BtsCI GGATG 4 cut(s) 223, 359, 392, 454
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 4 cut(s) 116, 168, 332, 363
Cac8I GCNNGC 1 cut(s) 135
Csp6I GTAC 1 cut(s) 86
CviAII CATG 4 cut(s) 134, 348, 427, 572
CviJI RGCY 5 cut(s) 30, 51, 236, 306, 576
CviKI_1 RGCY 5 cut(s) 30, 51, 236, 306, 576
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 4 cut(s) 108, 207, 246, 566
DpnI GATC 4 cut(s) 72, 106, 222, 331
DpnII GATC 4 cut(s) 70, 104, 220, 329
EaeI YGGCCR 2 cut(s) 49, 234
EagI CGGCCG 1 cut(s) 234
EclXI CGGCCG 1 cut(s) 234
Eco147I AGGCCT 1 cut(s) 306
Eco31I GGTCTC 2 cut(s) 464, 502
Eco52I CGGCCG 1 cut(s) 234
Eco81I CCTNAGG 1 cut(s) 566
FaeI CATG 4 cut(s) 137, 351, 430, 575
FalI AAGNNNNNCTT 2 cut(s) 291, 323
FatI CATG 4 cut(s) 133, 347, 426, 571
FauI CCCGC 1 cut(s) 210
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 2 cut(s) 52, 577
FokI GGATG 4 cut(s) 210, 346, 399, 461
Fsp4HI GCNGC 2 cut(s) 52, 577
GluI GCNGC 2 cut(s) 52, 577
GsuI CTGGAG 1 cut(s) 115
HaeIII GGCC 3 cut(s) 51, 236, 306
HapII CCGG 1 cut(s) 233
Hin1II CATG 4 cut(s) 137, 351, 430, 575
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HpaII CCGG 1 cut(s) 233
Hpy166II GTNNAC 3 cut(s) 6, 25, 377
Hpy188I TCNGA 2 cut(s) 285, 415
Hpy188III TCNNGA 3 cut(s) 266, 299, 565
Hpy8I GTNNAC 3 cut(s) 6, 25, 377
HpyAV CCTTC 2 cut(s) 296, 305
HpyCH4III ACNGT 2 cut(s) 163, 374
HpyCH4V TGCA 5 cut(s) 137, 261, 368, 488, 579
HpyF3I CTNAG 4 cut(s) 108, 207, 246, 566
Hsp92II CATG 4 cut(s) 137, 351, 430, 575
Kzo9I GATC 4 cut(s) 70, 104, 220, 329
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 1 cut(s) 563
LweI GCATC 1 cut(s) 406
MaeIII GTNAC 2 cut(s) 201, 518
MalI GATC 4 cut(s) 72, 106, 222, 331
MboI GATC 4 cut(s) 70, 104, 220, 329
MboII GAAGA 6 cut(s) 339, 544, 547, 550, 553, 556
MfeI CAATTG 2 cut(s) 256, 580
MflI RGATCY 2 cut(s) 70, 104
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 3 cut(s) 256, 490, 580
MnlI CCTC 6 cut(s) 120, 133, 186, 469, 502, 561
MseI TTAA 1 cut(s) 435
MspI CCGG 1 cut(s) 233
MunI CAATTG 2 cut(s) 256, 580
Mva1269I GAATGC 1 cut(s) 261
NdeII GATC 4 cut(s) 70, 104, 220, 329
NlaIII CATG 4 cut(s) 137, 351, 430, 575
NlaIV GGNNCC 2 cut(s) 72, 392
NmuCI GTSAC 1 cut(s) 518
NspI RCATGY 1 cut(s) 137
PaeI GCATGC 1 cut(s) 137
PceI AGGCCT 1 cut(s) 306
PctI GAATGC 1 cut(s) 261
PkrI GCNGC 2 cut(s) 53, 578
PspN4I GGNNCC 2 cut(s) 72, 392
PstI CTGCAG 1 cut(s) 370
PsuI RGATCY 2 cut(s) 70, 104
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 2 cut(s) 52, 577
Sau3AI GATC 4 cut(s) 70, 104, 220, 329
SduI GDGCHC 1 cut(s) 255
SetI ASST 4 cut(s) 32, 144, 208, 520
SfaNI GCATC 1 cut(s) 406
SfcI CTRYAG 1 cut(s) 366
SphI GCATGC 1 cut(s) 137
Sse9I AATT 3 cut(s) 256, 490, 580
SseBI AGGCCT 1 cut(s) 306
SsiI CCGC 2 cut(s) 52, 217
SspI AATATT 1 cut(s) 410
StuI AGGCCT 1 cut(s) 306
TaaI ACNGT 2 cut(s) 163, 374
TaqII GACCGA 1 cut(s) 488
TasI AATT 3 cut(s) 256, 490, 580
TatI WGTACW 1 cut(s) 85
TauI GCSGC 1 cut(s) 54
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 4 cut(s) 116, 168, 339, 370
TseFI GTSAC 1 cut(s) 518
TseI GCWGC 1 cut(s) 576
Tsp45I GTSAC 1 cut(s) 518
TspRI CASTG 4 cut(s) 116, 168, 339, 370
XapI RAATTY 1 cut(s) 490
XceI RCATGY 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.